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Genetic diversity for mycorrhizal symbiosis and phosphate transporters in rice

Journal of Integrative Plant Biology 2015年第11期57卷 969-979页

作者： Kwanho Jeong Nicolas Mattes Sheryl Catausan Joong Hyoun Chin Uta Paszkowski sigrid heuer Southern Cross Plant Science Southern Cross University International Rice Research Institute(IRRI) Crop and Environmental Sciences Division Seoul National University College of Agriculture and Life SciencesPlant Genomics and Breeding Institute University of Cambridge Department of Plant Sciences Australian Center For Plant Functional Genomics(ACPFG)

Phosphorus （P） is a major plant nutrient and developing crops with higher P-use efficiency is an important breeding goal. In this context we have conducted a comparative study of irrigated and rainfed rice varieties to assess genotypic differences in colonization with arbuscular mycorrhizal （AM） fungi and expression of different P trans- porter genes. Plants were grown in three different soil samples from a rice farm in the Philippines. The data show that AM symbiosis in all varieties was established after 4 weeks of growth under aerobic conditions and that, in soil derived from a rice paddy, natural AM populations recovered within 6 weeks. The analysis of AM marker genes （AM1, AM3, AM14） and P transporter genes for the direct Pi uptake （PT2, PT6） and AM-mediated pathway （PTll, PT13） were largely in agreement with the observed root AM colonization providing a useful tool for diversity studies. Interestingly, delayed AM colonization was observed in the aus-type rice varieties which might be due to their different root structure and might confer an advantage for weed competition in the field. The data further showed that P-starvation induced root growth and expression of the high-affinity P transporter PT6 was highest in the irrigated variety IR66 which a]so maintained grain yield under P-deficient field conditions.

关键词： Mycorrhiza P deficiency tolerance phosphatetransporters rice roots

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Transition from a maternal to external nitrogen source in maize seedlings

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Journal of Integrative Plant Biology 2017年第4期59卷 261-274页

作者： Kasra Sabermanesh Luke R. Holtham Jessey George Ute Roessner Berin A. Boughton sigrid heuer Mark Tester Darren C. Plett Trevor P. Garnett Australian Centre for Plant Functional Genomics Waite Research Institute University of Adelaide Adelaide SA 5064 Australia School of Agriculture Food and Wine Waite Research Institute University of Adelaide Adelaide SA 5064 Australia Australian Centre for Plant Functional Genomics School of BioSciences University of Melbourne Parkville Vic. 3010 Australia Metabolomics Australia School of BioSciences University of Melbourne Vic. 3010 Australia King Abdullah University of Science and Technology Center for Desert Agriculture Thuwal 23955-6900 Kingdom of Saudi Arabia6. The Australian Plant Phenomics Facility The Plant Accelerator Waite Campus The University of Adelaide Adelaide SA 5064 Australia The Australian Plant Phenomics Facility The Plant Accelerator Waite Campus The University of Adelaide Adelaide SA 5064 Australia

Maximizing NO3 uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 uptake and metabolism, to gain an understanding of how the NO3 uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seedderived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition （DAI）. Similarly, shoot N% decreases, but does not stabilize until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3- uptake capacity increases until shoot N% stabilizes. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3- uptake capacity to meet N demand provide an insight into the processes controlling N uptake.

关键词： NRT Transition from a maternal to external nitrogen source in maize seedlings NO

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The bumpy road of purinergic inhibitors to clinical application in immune-mediated diseases

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Neural Regeneration Research 2024年第6期19卷 1206-1211页

作者： Matthias T.Wyss Christine heuer Marina Herwerth Institute of Pharmacology and Toxicology University of ZurichZürichSwitzerland Neuroscience Center Zurich(ZNZ) University of Zurich and ETH ZurichZürichSwitzerland Neurology Department University Hospital of ZurichZürichSwitzerland

Purinergic signaling plays important roles throughout the body in the regulation of organ functions during and following the disruption of *** is also reflected by the widespread expression of two families of purinergic receptors(P1 and P2)with numerous *** the last few decades,there has been increasing evidence that purinergic signaling plays an important role in the regulation of immune ***,signals mediated by P2 receptors have been shown to contribute to immune system-mediated ***,interference with P2 receptors may be a promising strategy for the modulation of immune *** only a few clinical studies have been conducted in isolated entities with limited success,preclinical work suggests that the use of P2 receptor inhibitors may bear some promise in various autoimmune *** the association of P2 receptors with several disorders from this field,the use of P2 receptor antagonists in clinical therapy is still very *** this narrative review,we briefly review the involvement of the purinergic system in immunological responses and clinical studies on the effect of purinergic inhibition on autoimmune *** then open the aperture a bit and show some preclinical studies demonstrating a potential effect of purinergic blockade on autoimmune *** suramin,a non-specific purinergic inhibitor,as an example,we further show that off-target effects could be responsible for observed effects in immunological settings,which may have interesting ***,we believe that it is worthwhile to further investigate this hitherto underexplored area.

关键词： autoimmune diseases neurological disorders purinergic system P2 receptor inhibitors suramin

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Small scale adeno-associated virusvector production for preclinical gene delivery based on chloroform precipitation

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Neural Regeneration Research 2022年第1期17卷 99-100页

作者： Marcus Davidsson Andreas heuer Molecular Neuromodulation Department of Experimental Medical SciencesLund UniversityLundSweden Behavioural Neuroscience Laboratory Department of Experimental Medical SciencesLund UniversityLundSweden

Gene therapy aims to introduce genetic information into a cell-type of interest to replace,correct,silence,or modify defective *** therapy in its broadest sense can theoretically prevent,halt,or cure any condition that affects *** addition to that,the introduction and/or manipulation of genes is one of the major research areas in biological sciences,aimed to deepen our knowledge on how biological systems *** advances have made it possible to induce changes ranging from manipulations of large stretches of the genome to the change of single *** gold-standard vehicles to bring this genetic information into the target cells are viral vectors.

关键词： replace Small mankind

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IN-LINE PARTICLE SIZING FOR PROCESS CONTROL IN NEW DIMENSIONS

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China Particuology 2004年第4期2卷 185-188页

作者： Wolfgang Witt Michael heuer Markus Schaller Sympatec GmbH System-Partikel-Technik Burgstter StraBe6 D-38678 Clausthal-Zellerfeld GermanySympatec GmbH System-Partikel-Technik Burgstter StraBe6 D-38678 Clausthal-Zellerfeld GermanySympatec GmbH System-Partikel-Technik Burgstter StraBe6 D-38678 Clausthal-Zellerfeld Germany

The combination of laser diffraction with upstream sampling realized a break-through for the in- and on-line particles size analysis in industrial applications. Today, the combination of representative sampling, dry dispersion, particle size analysis by laser diffraction and integrated feedback of the sample is well accepted in many industrial applications. No more interactions of the user are required, and for standard applications the on-line monitoring of particle sizes became nearly as simple as the monitoring of any other process parameter. The increase of inspection interval from 24-hour operation to months has increased user confidence in this technology, and industries with more demanding measurement requirements are seeking to benefit from this performance. This challenge could not be solved with simple scale-ups or scale-downs. New solutions had to be found for the sampling system, the measuring sensor, the adaptation to the environmental conditions and the processing of fast growing volume of data.

关键词： in-line particle size analysis

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A platform for high-repetition-rate laser experiments on the Large Plasma Device

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High Power Laser Science and Engineering 2018年第2期6卷 32-37页

作者： D.B.Schaeffer L.R.Hofer E.N.Knall E V.heuer C.G.Constantin C.Niemann Department of Physics and Astronomy University of California-Los AngelesLos AngelesCA 90095USA

We present a new experimental platform for studying laboratory astrophysics that combines a high-intensity, highrepetition-rate laser with the Large Plasma Device at the University of California, Los Angeles. To demonstrate the utility of this platform, we show the first results of volumetric, highly repeatable magnetic field and electrostatic potential measurements, along with derived quantities of electric field, charge density and current density, of the interaction between a super-Alfv′enic laser-produced plasma and an ambient, magnetized plasma.

关键词： high energy density physics plasmas astrophysics

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Earth System Governance in South Asia： Designing Cross-Sector Collaboration

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Journal of Modern Accounting and Auditing 2012年第1期8卷 128-135页

作者： Mark heuer Amresh Kumar Susquehanna University Selinsgrove USA

The example of the U.S. and India is provided to define developing countries, with cross-sector collaboration sustainability in South Asia. Earth system governance sustainability and the overarching goal of providing generations the current geopolitical situation between developed and identified as a strategy for addressing environmental is proposed as a structure for addressing environmental an environment in South Asia that can sustain future

关键词： earth system governance cross-sector collaboration environmental sustainability

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A simple guideline to apply excitation-emission matrix spectroscopy(EEMs)for the characterization of dissolved organic matter(DOM)in anoxic marine sediments

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Acta Oceanologica Sinica 2023年第1期42卷 109-119页

作者： Shuchai Gan Verena Bheuer Frauke Schmidt Lars Wörmer Kai-Uwe Hinrichs MARUM—Center for Marine Environmental Sciences University of BremenBremenD-28359Germany

Marine sediments represent a major carbon reservoir on *** organic matter(DOM)in pore waters accumulates products and intermediates of carbon cycling in *** application of excitation-emission matrix spectroscopy(EEMs)in the analysis of subseafloor DOM samples is largely unexplored due to the redox-sensitive matrix of anoxic pore ***,this study aims to investigate the interference caused by the matrix on EEMs and propose a guideline to prepare pore water samples from anoxic marine *** parameters determined by fluorescence spectra include 3D-index derived from EEMs after parallel factor analysis(PARAFAC),fluorescence index(FI)(contribution of terrigenous DOM),biological index(BIX)and humification index(HIX)derived from 2D emission ***,we investigated the impacts of extensively-presented ions as typical electron acceptors,which are utilized by anaerobic microbes and stratified in marine sediments:Fe(II),Fe(III),Mn(II)and sulfide in anoxic pore water resulted in biases of fluorescent *** proposed threshold concentrations of these ions when the interference on EEMs *** removal of sulfide from sulfide-rich samples could be achieved by flushing with N_(2)for 2 ***,the tests based on DOM standard were further verified using pristine samples from marine *** was a significant change in the fluorescence spectra of DOM in anoxic sediments from the Rhône *** study demonstrated that the change was caused by oxidation of the matrix rather than the intrinsic alteration of *** was confirmed by extracted DOM via both EEMs analysis and Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR-MS).Slight oxidation of sulfur-containing compounds(e.g.,sulfhydryl)and polyphenol-like compounds ***,a sample preparation sequence is proposed for pore water from anoxic *** method enables measurement with small volumes of the sample(e.g.,50µL in this study)an

关键词： marine subsurface sediment EEMs PARAFAC FT-ICR-MS anaerobic pore water

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