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A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells

Microsystems & Nanoengineering 2019年第1期5卷 593-605页

作者： Yue Yu Richard P.Sde Campos Seolim Hong Dimitar L.Krastev siddharth sadanand Yen Leung Aaron R.Wheeler Institute for Biomaterials and Biomedical Engineering University of Toronto164 College StTorontoON M5s 3G9Canada Donnelly Centre for Cellular and Biomolecular Research 160 College St.TorontoON M5S 3E1Canada Department of Chemistry University of Toronto80 St George St.TorontoON M5S 3H6Canada Department of Human Biology University of Toronto300 Huron StreetTorontoON M5S 3J6Canada

Homeostasis of dopamine,a classical neurotransmitter,is a key indicator of neuronal *** in the regulation of dopamine is implicated in a long list of neurological disorders,including addiction,depression,and *** existing methods used to evaluate dopamine homeostasis in vitro are inconvenient and do not allow for continuous non-destructive *** response to this challenge,we introduce an integrated microfluidic system that combines dopaminergic cell culture and differentiation with electroanalytical measurements of extracellular dopamine in real-time at any point during an *** used the system to examine the behavior of differentiated SH-SY5Y cells upon exposure to four dopamine transporter ant/agonists(cocaine,ketamine,epigallocatechin gallate,and amphetamine)and study their *** IC_(50)values of cocaine,ketamine,and epigallocatechin gallate were determined to be(average±standard deviation)3.7±1.1μM,51.4±17.9μM,and 2.6±0.8μM,***,we used the new system to study amphetamine-mediated dopamine release to probe the related phenomena of dopamine transporter-mediated reverse-transport and dopamine release from *** propose that this platform,which is the first platform to simultaneously evaluate uptake and release,could be useful to screen for drugs and other agents that target dopaminergic neurons and the function of the dopamine *** broadly,this platform should be adaptable for any application that could benefit from high-temporal resolution electroanalysis combined with multi-day cell culture using small numbers of cells.

关键词： dopamine homeostasis release

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Correction to:A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells

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Microsystems & Nanoengineering 2019年第1期5卷 514-514页

Correction to:Microsystems&Nanoengineering(2019)5:10 https://***/10.1038/s41378-019-0049-2 published online:11 March 2019 The legend in Fig.4a in the previously published version of this article contained erroneous *** correct units are given in the caption–that is,1μM(black),500 nM(red),100 nM(blue),50 nM(cyan),10 nM(pink),and 0 nM(1×PBS solution,brown).

关键词： homeostasis brown monitoring

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Comparison of Ground Water Bacterial Cell Sizes from the Agricultural, Domestic and Industrial Areas of Mysore District, Karnataka State, India

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应用与环境生物学报 2008年第4期14卷 503-509页

作者： Wadie Ahmed Mokbel sadanand M Yamakanamardi Aquatic Microbial Ecology Research Laboratory Department of Studies in Zoology University of Mysore Manasagangotri Mysore-570006 Karnataka State India

A two-year study on temporal variations in the ground water heterotrophic bacterial cell sizes of free living bacteria (FLB) and particle bound bacteria (PBB) from the agricultural, domestic and industrial areas was carried out from February 2005 to January 2007. The overall mean cell length of FLB and PBB was similar in all the ground water studied. However, the season wise grouped data revealed significant seasonal changes in cell length of FLB and PBB, as smaller bacteria were noticed during rainy season in the ground water in agricultural area in both the years, and only in the second year of study in domestic and industrial areas. Generally, it was noticed that there were summer maximum and rainy minimum values of the cell length of PBB in the ground water in agricultural, domestic and industrial areas in the second year of study. The Pearson's correlations showed the presence of 8 (in agricultural area), 5 (in domestic) and 3 (in industrial) significant correlations with environmental (Physico-chemical) parameters, respectively. The regression analysis revealed that as much as 12% of variation in the mean length of FLB was due to NO3 (+) in agricultural area and 9% due to total solids (+) in domestic area. However, the 8 % variation in bacterial cell size of FLB was due to Mg (+) in industrial area. Whereas, 13 % variation in mean length of PBB was due to SO4 (+) in agricultural area and 10% due to total anions of strong acid (TASA) (+) in domestic area. Furthermore, 10 % of variation was due to PO4 (+) in industrial area. Thus, the statistical analysis revealed that several environmental variables were potentially responsible for some of the temporal variations in aquatic heterotrophic bacterial cell size, suggesting probably the stressed environment in these ecosystems.

关键词：地下水微生物细胞环境保护

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Preparation and properties of cellulose nanocomposite fabrics with in situ generated silver nanoparticles by bioreduction method

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Journal of Bioresources and Bioproducts 2021年第1期6卷 75-81页

作者： Battu Deeksha Vajja sadanand NHariram Anumakonda Varada Rajulu Department of Chemical Engineering Osmania UniversityHyderabad500007India Department of Chemistry Osmania UniversityHyderabad500007India Department of Biochemistry Kalasalingam UniversityAnand NagarKrishnankovil626126India Centre for Composite Materials International Research CentreKalasalingam UniversityAnand NagarKrishnankovil 626126India

The aim of the present study was to develop antibacterial cellulose(cotton)nanocomposite fab-rics(CNCFs)with in situ generated silver nanoparticles using medicinal plant Vitex leaf *** developed CNCFs were characterized by scanning electron microscope(SEM),Fourier transform infrared(FT-IR)spectroscopy,X-ray diffraction(XRD)and antibacterial ***,these CNCFs possessed good antibacterial *** CNCFs prepared using simple and environmentally friendly method can be considered for medical applications in,such as,surgical aprons,wound cleaning,wound dressing,and hospital bed materials.

关键词： Cotton fabrics Silver nanoparticles In situ generation Antibacterial activity

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Temporal and spatial control of gene expression inhorticultural crops

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Horticulture Research 2014年第1期1卷 69-85页

作者： Manjul Dutt sadanand A Dhekney Leonardo Soriano Raju Kandel Jude W Grosser Citrus Research and Education Center University of Florida700 Experiment Station RoadLake AlfredFL 33850USA Department of Plant Sciences Sheridan Research and Extension CenterUniversity of WyomingSheridanWY 82801USA Universidade de Sao Paulo Centro de Energia Nuclear na AgriculturaPiracicabaBrazil

Biotechnology provides plant breeders an additional tool to improve various traits desired by growers and consumers of horticultural *** also provides genetic solutions to major problems affecting horticultural crops and can be a means for rapid improvement of a *** the availability of a number of horticultural genome sequences,it has become relatively easier to utilize these resources to identify DNA sequences for both basic and applied *** play a key role in plant gene expression and the regulation of gene *** recent years,rapid progress has been made on the isolation and evaluation of plant-derived promoters and their use in horticultural crops,as more and more species become amenable to genetic *** understanding of the tools and techniques of horticultural plant biotechnology has now evolved from a discovery phase to an implementation *** availability of a large number of promoters derived from horticultural plants opens up the field for utilization of native sequences and improving crops using precision *** this review,we look at the temporal and spatial control of gene expression in horticultural crops and the usage of a variety of promoters either isolated from horticultural crops or used in horticultural crop improvement.

关键词： expression. crops utilize

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Synthesis of Novel Nanocomposites of Silica and Guar Gum:Efficient Zinc Ion Binder

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复旦学报（自然科学版） 2007年第5期46卷 721-722页

作者： Vandana Singh sadanand Pandey Somit kumar Singh Rashmi Sanghi Department of Chemistry University of Allahabad 302 Southern Laboratories Facility for Ecological and Analytical TestingIndian Institute of Technology

1 Results Adsorption using commercial activated carbon (CAC)[1] can remove heavy metals from wastewater[2-4].However,CAC remains an expensive material for heavy metal *** exchange techniques are also used but it generates volumetric sludge and increases the cost[5].Using naturally occurring seed polysaccharides can solve these problems but their solubility in water limits its application as an *** gum is a commercially available,industrially important[6-7] polysaccharide material havi...

关键词： nanocomposite silica guar gum zinc ion binder

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New Formulas and Results for 3-Dimensional Vector Fields

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Applied Mathematics 2021年第11期12卷 1058-1096页

作者： sadanand D. Agashe 2-B Anjaneya Society Mumbai India

New formulas are derived for once-differentiable 3-dimensional fields, using the operator . This new operator has a property similar to that of the Laplacian operator;however, unlike the Laplacian operator, the new operator requires only once-differentiability. A simpler formula is derived for the classical Helmholtz decomposition. Orthogonality of the solenoidal and irrotational parts of a vector field, the uniqueness of the familiar inverse-square laws, and the existence of solution of a system of first-order PDEs in 3 dimensions are proved. New proofs are given for the Helmholtz Decomposition Theorem and the Divergence theorem. The proofs use the relations between the rectangular-Cartesian and spherical-polar coordinate systems. Finally, an application is made to the study of Maxwell’s equations.

关键词： Spherical-Polar Coordinates Helmholtz Decomposition Divergence Theorem Orthogonality Maxwell’s Equations

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Profiles of HIV-infected anti-retroviral therapy naïve children from Mumbai,India

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World Journal of Pediatrics 2016年第4期12卷 430-435页

作者： Supriya Mayur Paranjpe Purva Pankaj Sarkate Nayana Avinash Ingole Shweta sadanand Raut Preeti Rajeev Mehta Department of Microbiology 5th floorMulti-Storey BuildingSeth G.S.Medical College and K.E.M.HospitalParelMumbaiIndia

Background:This study aimed to investigate the demographic profiles of human immunodifficiency virus(HIV)infected anti-retroviral therapy(ART)naïve children in our hospital and their relations to the clinical,immunological and nutritional ***:A cross-sectional study was conducted in an Integrated Counselling and Testing Center(ICTC)at a tertiary care hospital in *** naïve HIV positive children were enrolled in the *** demographic profiles,clinical features,immunological(CD4%/CD4 count)and nutritional status of these children were *** agreement between clinical,immunological and nutritional staging was determined using Cohen’s kappa ***:In 192 HIV-infected ART naive children enrolled with a median age of 9 years(range 3 months-14 years),97.4%acquired infection through vertical *** most common clinical presentation was fever(39.6%),followed by generalized lymphadenopathy(32.3%),cough(22.4%)and diarrhoea(9.9%).Tuberculosis was seen in 22.9%of the *** agreement was fair between clinical and immunological staging,and slight between nutritional,immunological and clinical ***:Perinatal transmission is the most common mode of acquiring HIV infection in *** Prevention of Parent to Child Transmission(PPTCT)program should be strengthened for lowering the transmission rate by providing extended ART to mothers during pregnancy and *** remains a major concern in HIV-infected *** poor correlation between WHO clinical and immunological staging emphasizes the importance of making CD4 facilities available in HIV prevalent *** cannot be used as a surrogate marker for predicting stage or severity as it is common at all stages of HIV disease.

关键词： anti-retroviral therapy CD4 count clinical stage HIV nutritional status

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An optimized procedure for plant recovery from somatic embryos significantly facilitates the genetic improvement of Vitis

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Horticulture Research 2014年第1期1卷 183-189页

作者： Zhijian T Li Kyung-Hee Kim sadanand A Dhekney Jonathan R Jasinski Matthew R Creech Dennis J Gray Grape Biotechnology Core Laboratory Mid-Florida Research and Education CenterUniversity of Florida/IFASApopkaFL 32703-8504USA Department of Plant Sciences Sheridan Research and Extension CenterSheridanWY 82801USA

Plant regeneration from grapevine(Vitis spp.)via somatic embryogenesis typically is *** of plants from Vitis rotundifolia Michx.(muscadine grape)is particularly problematic due to extremely low efficiency,including extended culture durations required for embryo–plant *** plant recovery is an obstacle to the selection of improved genetically modified *** embryos(SEs)of *** cultivar Delicious(Del-HS)and Vitis vinifera L cultivar Thompson Seedless(TS)were used to identify culture media and conditions that promoted embryo differentiation and plant conversion;this resulted in a two-step culture *** comparative culture experiments,C2D medium containing 6%sucrose was the most effective,among four distinct formulae tested,for inducing precocious SE germination and cell *** medium,further supplemented with 4 mM 6-benzylaminopurine(C2D4B),was subsequently determined to enhance post-germinative growth of *** medium supplemented with 0.5 mM 1-naphthaleneacetic acid(MSN)was then utilized to stimulate root and shoot growth of germinated *** average of 35%and 80%‘Del-HS’and‘TS’SE,respectively,developed into *** plants developed robust root and shoot systems and exhibited excellent survival following transfer to *** 150 plants of‘Del-HS’were regenerated and established within 2.5 months,which is a dramatic reduction from the 6-to 12-month time period previously ***,88‘TS’plant lines were obtained within the same time ***,seven out of eight Vitis cultivars exhibited significantly increased plant conversion percentages,demonstrating broad application of the two-step culture system to produce the large numbers of independent plant lines needed for selection of desired traits.

关键词： cultivar Vitis culture

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Clonal Fidelity of Micropropagated Psidium guajava L. Plants Using Microsatellite Markers

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American Journal of Plant Sciences 2015年第14期6卷 2385-2392页

作者： Bernessa Rawls Karen Harris-Shultz sadanand Dhekney Ivis Forrester Viji Sitther Feagin Mill Middle School Warner Robins GA USA USDA Crop Genetics and Breeding Research Unit Tifton GA USA Sheridan Research and Extension Center University of Wyoming Sheridan WY USA School of Community Health and Policy Morgan State University Baltimore MD USA Department of Biology Morgan State University Baltimore MD USA

Micropropagation of Psidium guajava L. (guava) is a viable alternative to currently adopted techniques for large-scale plant propagation of commercial cultivars. Assessment of clonal fidelity in micropropagated plants is the first step towards ensuring genetic uniformity in mass production of planting material. In the present study, 31 plants of guava cultivar “Lucknow 49” regenerated by micropropagation were tested for genetic fidelity by comparing them to the mother plant from which explant material was obtained. Efficient rooting of in vitro proliferated shoots was obtained by culture on 1/2 strength MS medium supplemented with either 9.8 μM indole butyric acid (IBA) or 11.4 μM indole acetic acid (IAA). Leaf samples of 31 regenerated plants were compared to the mother plant using 17 simple sequence repeat (SSR) markers. While 16 SSRs detected the same allele, locus mPgCIR07 detected slight differences, where six micropropagated plants were 1 bp smaller (152 bp) than the parental genotype (153 bp). Differences in leaf tissues for anthocyanin pigmentation were also noted among micropropagated plants. Results of the study indicated efficient rooting of “Lucknow-49” cultivar for rapid propagation of planting material, and revealed that micropropagated plants were identical for 16 of the 17 loci examined. Although most mutations induced by tissue culture may not have an effect on phenotype, the possibility that novel phenotypes can be generated in a commercial setting exists.

关键词： Genetic Stability Guava Simple Sequence Repeats Tissue Culture

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