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小檗碱联合姜黄素调控M1巨噬细胞极化对动脉粥样硬化的作用机制

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实用医学杂志 2024年第14期40卷 1915-1921页

作者：陈玉善王婷婷韩心怡华成俊靳博远尚莎莎宗永华梁亚州河南中医药大学第一附属医院心脏中心郑州450000 河南中医药大学第一临床医学院郑州450000

目的探究小檗碱联合姜黄素通过介导巨噬细胞向M1极化对动脉粥样硬化(AS)的作用及机制。方法常规培养小鼠单核巨噬细胞(RAW264.7),通过脂多糖(LPS,100 ng/mL)和γ干扰素(IFN-γ)(20 ng/mL)诱导巨噬细胞RAW264.7获得M1型巨噬细胞,建立细... 详细信息

目的探究小檗碱联合姜黄素通过介导巨噬细胞向M1极化对动脉粥样硬化(AS)的作用及机制。方法常规培养小鼠单核巨噬细胞(RAW264.7),通过脂多糖(LPS,100 ng/mL)和γ干扰素(IFN-γ)(20 ng/mL)诱导巨噬细胞RAW264.7获得M1型巨噬细胞,建立细胞模型。将细胞分为对照组、模型组、小檗碱组、姜黄素组、小檗碱+姜黄素联合治疗组。通过CCK-8方法检测小檗碱和姜黄素的处理浓度。ELISA检测各组巨噬细胞上清液M1型巨噬细胞标记物iNOS、TNF-α、CXCL9和p-STAT6/STAT6的表达水平;RT-PCR方法检测iNOS、TNF-α和CXCL9 mRNA水平;Western blot检测各组iNOS、STAT6和p-STAT6蛋白水平。此外,通过siRNA技术下调STAT6水平后,通过Western blot检测p-STAT6蛋白水平的表达;ELISA检测iNOS、TNF-α、CXCL9和p-STAT6表达水平。结果LPS和IFN-γ联合诱导M1巨噬细胞极化中,相较于其他药物浓度组比,小檗碱(25μmol/L)、姜黄素(20μmol/L)为最佳药物浓度,两者单用和联用均不能显著抑制RAW264.7细胞活力(P<0.05)。与正常组比较,模型组iNOS、TNF-α和CXCL9 mRNA和蛋白水平均升高,p-STAT6/STAT6水平降低,差异有统计学意义(P<0.05);与模型组相比,小檗碱组、姜黄素组、小檗碱+姜黄素联合治疗组中iNOS、TNF-α和CXCL9 mRNA和蛋白水平均降低,p-STAT6/STAT6水平升高,且小檗碱+姜黄素联合治疗组中变化的更加明显,差异有统计学意义(P<0.05)。在小檗碱+姜黄素联合治疗组的M1型巨噬细胞中转染STAT6 siRNA后可下调p-STAT6水平,上调iNOS、TNF-α和CXCL9表达,差异有统计学意义(P<0.05)。结论小檗碱和姜黄素对M1型巨噬细胞的活性均有抑制作用,能够减轻炎症反应。此外,小檗碱与姜黄素联合作用较单药使用更有优势,可能是通过活化STAT6发挥其主要作用机制。

关键词：动脉粥样硬化 M1型巨噬细胞小檗碱姜黄素 STAT6

Rapamycin combined with allogenic immature dendritic cells selectively expands CD4^+CD25^+Foxp3^+ regulatory T cells in rats

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Hepatobiliary & Pancreatic Diseases International 2012年第2期11卷 203-208页

作者： Guo-Ying Wang Qi Zhang Yang Yang Wen-Jie chen Wei Liu Nan Jiang Gui-hua chen chgh1955@*** Liver Transplantation Center Third Affiliated Hospital Transplantation Research Institute Sun Yat-Sen University Organ Transplantation Research Center of Guangdong Province Guangzhou 510630 China

ReferencesReferencesBackgroundDendritic cells (DCs) can initiate the expansion of regulatory T cells (Tregs), which play an indispensable role in inducing transplantation tolerance. Some studies have investigated the effect of the immunosuppressant rapamycin (Rapa) on Tregs in vitro. However, the in vivo effect of Rapa combined with immature DCs (iDCs) on Tregs is unknown. This study was undertaken to determine whether allogenic iDCs combined with a short course of Rapa have the ability to selectively expand the CD4+CD25+Foxp3+ Tregs in a rat *** Norway rats were injected intravenously with 2×106 Lewis iDCs followed by 1 mg/kg per day Rapa intraperitoneally for 7 consecutive days. On day 8, the levels of CD4+CD25+Foxp3+ Treg cells in peripheral blood and spleen cells were analyzed by flow cytometry. IL-2, IL-4, TGF-β1, and IFN-γ levels in serum were assessed by ELISA. The experimental animals were divided into four groups: control, Rapa-treated, iDC-treated, and ***4+CD25+Foxp3+ Tregs comprised 7%-8%of CD4+ T cells in control rats. Rapa combined with iDCs enhanced this percentage in the peripheral blood and spleen. However, the levels of Tregs did not significantly change after treatment with Rapa or iDCs alone. The levels of CD4+CD25?/sup>Foxp3+ T cells and CD4+ CD25+Foxp3?/sup> T cells in CD4+ T cells did not significantly change in the combined group. The TGF-β1 level in serum from the combined group increased significantly compared with the other *** significantly higher percentage of CD4+ CD25+ Foxp3+ Tregs was found in rats treated with allogenic iDCs and a short course of Rapa, along with an increase in the TGF-β1 level in serum. This improved protocol may be a promising therapeutic strategy to increase Tregs, which are beneficial to the induction of peritransplant tolerance.

关键词： dendritic cells regulatory T cells rapamycin

Tailoring grain morphology in Ti-6Al-3Mo through heterogeneous nucleation in directed energy deposition

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Journal of Materials Science & Technology 2021年第29期88卷 132-142页

作者： Fengying Zhang Panpan Gao hua Tan Yao Li Yongnan chen Min Mei Adam T.Clare Lai-Chang Zhang School of Material Science and Engineering Chang’an UniversityXi’an710064China State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an710072China Advanced Component Engineering Laboratory Faculty of EngineeringUniversity of NottinghamNG72RDUnited Kingdom School of Engineering Edith Cowan University270 Joondalup DriveJoondalupPerthWA6027Australia

A common challenge in direct energy deposition(DED)is eliminating the anisotropy in mechanical performance associated with microstructure and the formation of coarse columnar *** this work,a heterogeneous nucleation mechanism was introduced into the melt pool,and,from this mechanism,an almost fully equiaxed grain morphology was obtained in the DED of Ti-6 Al-3 *** types of grain morphologies in DED Ti-6 Al-3 Mo,including full columnar grains,near-equiaxed grains and almost fully equiaxed grains were obtained from premixed and satellite powder blends from Ti,6 wt.%Al and 3 wt.%Mo,*** with the analysis of the interactions between powder particles and the melt pool in DED,the formation mechanism of the equiaxed grains caused by the incomplete melting of high melting point Mo particles was *** the prior-βgrains transformed from coarse columnar grains to fine-equiaxed grains,the strongfiber texture along the deposition direction was weakened,while the size of theα-laths in the prior-βgrains slightly decreased,and the selection ofα-variants was *** to the transformation of the prior-βgrains from coarse columnar grains to fine-equiaxed grains,the tensile strength of the deposited samples increased from 982 MPa to 1082 MPa,while the yield strength increased from 840 MPa to 922 MPa,and the elongation of the as-deposited alloy also increased from 9.0%to 9.8%,which confirmed that the presence of fine-equiaxed grains is beneficial to the strength and plasticity of the DED *** work further demonstrates the role that satelliting powders can play in terms of enhancing the columnar to equiaxed transition(CET)behavior associated with DED.

关键词： Direct energy deposition Titanium alloy Grain morphology Microstructure Mechanical properties

Chromosome-level genome assembly of Cylas formicarius provides insights into its adaptation and invasion mechanisms

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Journal of Integrative Agriculture 2023年第3期22卷 825-843页

作者： hua Jin-feng ZHANG Lei HAN Yong-hua GOU Xiao-wan chen Tian-yuan huaNG Yong-mei LI Yan-qing MA Dai-fu LI Zong-yun Institute of Integrative Plant Biology/Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics/School of Life Sciences Jiangsu Normal UniversityXuzhou 221116P.R.China Sweet Potato Laboratory Institute of Maize ResearchGuangxi Academy of Agricultural SciencesNanning 530007P.R.China Xuzhou Academy of Agricultural Sciences/Institute of Sweet Potato Research Chinese Academy of Agricultural SciencesXuzhou 221131P.R.China

Cylasformicarius is one of the most important pests of sweet potato worldwide, causing considerable ecological and economic *** study improved the effect of comprehensive management and understanding of genetic mechanisms by examining the functional genomics of C. *** Illumina and PacBio sequencing, this study obtained a chromosome-level genome assembly of adult weevils from lines inbred for 15 *** high-quality assembly obtained was 338.84 Mb, with contig and scaffold N50 values of 14.97 and 34.23 Mb, *** total, 157.51 Mb of repeat sequences and 11 907 protein-coding genes were predicted.A total of 337.06 Mb of genomic sequences was located on the 11 chromosomes, accounting for 99.03%of the total length of the associated *** genomic analysis showed that C. formicarius was sister to Dendroctonus ponderosae, and C. formicarius diverged from D. ponderosae approximately 138.89 million years ago (Mya).Many important gene families expanded in the C. formicarius genome were involved in the detoxification of pesticides, tolerance to cold stress and chemosensory *** further study the role of odorant-binding proteins (OBPs) in olfactory recognition of C. formicarius, the binding assay results indicated that Cfor OBP4–6 had strong binding affinities for sex pheromones and other *** high-quality C. formicarius genome provides a valuable resource to reveal the molecular ecological basis, genetic mechanism, and evolutionary process of major agricultural pests;it also offers new ideas and new technologies for ecologically sustainable pest control.

关键词： Cylas formicarius PacBio sequencing high-through chromosome conformation capture chromosome-level genome chemosensory genes fluorescence competitive binding

污废水中污染物去除的功能材料研究进展:元素物质-合成改性-工艺工程的尺度效应

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应用化学 2024年第2期41卷 190-216页

作者：庞子君覃智陈啊聪关翔鸿韦庚锐李泽敏黄华韦朝海华南理工大学环境与能源学院广州510006

污废水中的有机污染物、重金属离子和病毒性微生物等造成了严重的水环境污染,对人体健康和生态系统也造成严重威胁,有效控制污废水的风险成为保护环境和可持续发展的重要研究方向。环境功能材料作为一类具有污染物净化效果的辅助性手段... 详细信息

污废水中的有机污染物、重金属离子和病毒性微生物等造成了严重的水环境污染,对人体健康和生态系统也造成严重威胁,有效控制污废水的风险成为保护环境和可持续发展的重要研究方向。环境功能材料作为一类具有污染物净化效果的辅助性手段,在降低水污染风险中展现出巨大的科技潜力。本研究探讨元素物质的选择、合成改性策略和工艺工程的优化3个尺度层面的现状与发展;以碳元素、硅元素和金属元素为例,介绍新型功能材料的特征;从溶剂热法、溶胶-凝胶法、共沉淀法、化学气相沉积法、绿色合成法以及表面功能化改性讨论了合成技术策略;关于工艺工程应用方面,固定床-吸附床-流化床、电场-光场-磁场、物理-化学-生物、循环-再生-处置和协同放大效应等的产业生产/连续运行方面值得关注;在性能、经济性、环境影响和可持续性方面进行评价,强调了安全-稳定-长效-满负荷-优化的工艺工程技术应用目标。未来的环境功能材料发展应聚焦3D打印材料在污废水处理中的应用、智能工艺和智慧管理、群体感知和信息诊断以及场域效应和量子探针等技术方向的结合,在水质改善-材料功能挖掘-可持续循环发展方面提供新的技术领域。

关键词：环境功能材料污废水处理元素物质的选择合成改性策略工艺工程应用可持续评价

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吴川市暴雨强度公式的推导及评估

广东气象 2024年第4期46卷 20-24页

作者：吴宗树陈志权罗舒萌郑华斌陈晓珠吴川市气象局广东吴川524500

为进一步做好城市排水系统的科学性和实用性,采用吴川市国家气象观测站1980—2021年逐分钟雨量数据资料,利用年最大值法、数值逼近和最小二乘法等,推导吴川市暴雨强度公式,结果表明:湛江市暴雨强度公式理论值与吴川市实际值误差太大,不... 详细信息

为进一步做好城市排水系统的科学性和实用性,采用吴川市国家气象观测站1980—2021年逐分钟雨量数据资料,利用年最大值法、数值逼近和最小二乘法等,推导吴川市暴雨强度公式,结果表明:湛江市暴雨强度公式理论值与吴川市实际值误差太大,不能达到室外排水规范的要求,而推导的暴雨强度区间参数公式和暴雨强度综合公式精度均符合精度要求,可作为相关部门设计吴川市排水基础设施的相关参考依据,且暴雨强度区间参数公式精度明显优于暴雨强度综合公式。

关键词：气候学暴雨强度公式年最大值法皮尔逊-Ⅲ型分布曲线吴川市

3’-Methoxydaidzein exerts analgesic activity by inhibiting voltage-gated sodium channels

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Chinese Journal of Natural Medicines 2019年第6期17卷 413-423页

作者： XU Run-Jia FEI shuo-Han chen Lin-Yan WANG Gan LIU Ming ZHANG Wen-Sheng YAN Xiu-Wen LAI Ren SHEN Chuan-Bin Life Sciences College of Nanjing Agricultural University Nanjing 210095China Key Laboratory of bioactive peptides of Yunnan Province/Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences Kunming Institute of ZoologyKunming 650223China Department of Molecular and Cell Biology School of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China Laboratory of Anesthesia and Critical Care Medicine Translational Neuroscience CenterWest China HospitalSichuan UniversityChengdu 610041China Sino-African Joint Research Center CASKunming Institute of ZoologyKunming 650223China

Isoflavones are widely consumed by people around the world in the form of soy products, dietary supplements and drugs. Many isoflavones or related crude extracts have been reported to exert pain-relief activities, but the mechanism remains unclear. Voltage-gated sodium channels(VGSCs) play important roles in excitability of pain sensing neurons and many of them are important nociceptors. Here, we report that several isoflavones including 3’-methoxydaidzein(3 MOD), genistein(GEN) and daidzein(DAI) show abilities to block VGSCs and thus to attenuate chemicals and heat induced acute pain or chronic constriction injury(CCI) induced pain hypersensitivity in mice. Especially, 3 MOD shows strong analgesic potential without inducing addiction through inhibiting subtypes NaV1.7, NaV1.8 and NaV1.3 with the IC50 of 181 ± 14, 397 ± 26, and 505 ± 46 nmol·L–1, respectively, providing a promising compound or parent structure for the treatment of pain pathologies. This study reveals a pain-alleviating mechanism of dietary isoflavones and may provide a convenient avenue to alleviate pain.

关键词： 3’-Methoxydaidzein Analgesic Isoflavone Chronic pain Sodium channel

Distribution of fatty acids in the alpine grassland soils of the Qinghai-Tibetan Plateau

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Science China Earth Sciences 2016年第7期59卷 1329-1338页

作者： DAI Guo hua ZHU Shan Shan LIU Zong Guang chen Li Tong HE Jin Sheng FENG Xiao Juan State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau Biology Chinese Academy of Sciences Department of Ecology College of Urban and Environmental Sciences Peking University

As an important biomarker, fatty acids(FAs) have been extensively used to trace the origin of organic matter in sediments and soils. However, studies of the distribution and abundance of FAs in alpine grassland soils are still rare, especially on the Qinghai-Tibetan Plateau(QTP), the highest plateau in the world, which contributes sediments to many large rivers in Asia. This study investigates the composition, distribution and source of FAs with increasing soil depths from 17 typical alpine grassland sites in the QTP. The most abundant FAs included the ubiquitous C16 FA and even-numbered long-chain FAs(C20–C30), indicating mixed inputs from microbial and higher plant sources. Source apportionment showed that higher plants were the dominant contributor of FAs(approximately 40%) in QTP soils. The abundance of FAs decreased with soil depth, with the highest value(1.08±0.09 mg/g C) at a 0–10 cm depth and the lowest value(0.46±0.12 mg/g C) at a 50–70 cm depth, due to much lower plant inputs into the deeper horizons. The total concentration of FAs was negatively correlated to the mean annual temperature(MAT; P<0.05) and soil p H(P<0.01), suggesting that the preservation of FAs was favored in low-MAT and low-p H soils on the QTP. The abundance of fresh C source FAs increased significantly with the mean annual precipitation(MAP; P<0.05), indicating that high MAP facilitates the accumulation of fresh FAs in QTP soils. Other environmental parameters, such as the soil mineral content(aluminum and iron oxide), microbial community composition as well as litter quality and quantity, may also exert a strong control on the preservation of FAs in QTP soils and warrant further research to better understand the mechanisms responsible for the preservation of FAs in QTP soils.

关键词： Qinghai-Tibetan Plateau Soil organic matter Biomarker Fatty acids Distribution sources

Autotaxin:An Early Warning Biomarker for Acute-on-chronic Liver Failure

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Journal of Clinical and Translational Hepatology 2020年第3期8卷 240-245页

作者： Caiyun Nie Lei Zhang Xiaobing chen Ying Li Fushuang Ha hua Liu Tao Han Department of Oncology The Affiliated Cancer Hospital of Zhengzhou UniversityZhengzhouHenanChina Henan Cancer Hospital ZhengzhouHenanChina Department of Clinical Laboratory Tianjin Third Central HospitalTianjinChina The Third Central Clinical College of Tianjin Medical University TianjinChina The Affiliated Hospital of Nankai University TianjinChina Department of Hepatology and Gastroenterology Tianjin Third Central HospitalTianjinChina Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases TianjinChina Artificial Cell Engineering Technology Research Center TianjinChina Tianjin Institute of Hepatobiliary Disease TianjinChina

Background and Aims:Recent accumulating evidence indicates the biological actions of autotaxin(ATX)in liver ***,the relationship between ATX and liver failure has not been *** present study aimed to examine alterations of serum ATX in acute-on-chronic liver failure(ACLF)and evaluate whether serum ATX could be useful as an early warning biomarker of ***:Serum ATX was measured in 50 patients with hepatitis B-related ACLF,14 patients with alcohol-related ACLF,11 patients with hepatitis B-related pre-ACLF,11 patients with alcohol-related Child-Pugh A cirrhosis,39 patients with hepatitis B-related Child-Pugh A cirrhosis,26 patients with chronic hepatitis B,and 38 healthy volunteers by enzyme-linked immunosorbent ***:Serum ATX level was significantly higher in the pre-ACLF group than in the Child-Pugh A cirrhosis and chronic hepatitis B groups but lower than in the ACLF group;furthermore,patients with pre-ACLF deteriorated to ACLF had significantly higher serum ATX levels than pre-ACLF patients that did not progress to *** ATX levels were significantly higher among male ACLF patients with preclinical infection,spontaneous bacterial peritonitis or pneumonia,as compared to patients with ACLF but no spontaneous bacterial peritonitis or *** ATX levels were well correlated with serum biochemical parameters of liver function and model for end-stage liver disease *** ATX≥584.1 ng/mL was a poor prognostic factor for ACLF(hazard ratio of 4.750,95%confidence interval of 1.106-20.392,p=0.036).Conclusions:Serum ATX level may be a useful early warning biomarker for ACLF.

关键词： Autotaxin(ATX) Liver failure Serum Biomarker