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Molecular Mechanisms of Intracellular Delivery of Nanoparticles Monitored by an Enzyme‑Induced Proximity Labeling

Nano-Micro Letters 2024年第6期16卷 14-37页

作者： Junji Ren Zibin Zhang shuo geng Yuxi Cheng Huize Han Zhipu Fan Wenbing Dai Hua Zhang Xueqing Wang Qiang Zhang Bing He Department of Pharmaceutics School of Pharmaceutical Sciences Peking University38 Xueyuan RdHaidian DistrictBeijing 100191People’s Republic of China

Achieving increasingly finely targeted drug delivery to organs,tissues,cells,and even to intracellular biomacromolecules is one of the core goals of *** the delivery destination is refined to cellular and subcellular targets,it is essential to explore the delivery of nanomedicines at the molecular ***,due to the lack of technical methods,the molecular mechanism of the intracellular delivery of nanomedicines remains unclear to ***,we develop an enzyme-induced proximity labeling technology in nanoparticles(nano-EPL)for the real-time monitoring of proteins that interact with intracellular ***(lactic-co-glycolic acid)nanoparticles coupled with horseradish peroxidase(HRP)were fabricated as a model(HRP(+)-PNPs)to evaluate the molecular mechanism of nano delivery in *** adding the labeling probe biotin-phenol and the catalytic substrate H_(2)O_(2)at different time points in cellular delivery,nano-EPL technology was validated for the real-time in situ labeling of proteins interacting with ***-EPL achieves the dynamic molecular profiling of 740 proteins to map the intracellular delivery of HRP(+)-PNPs in macrophages over *** on dynamic clustering analysis of these proteins,we further discovered that different organelles,including endosomes,lysosomes,the endoplasmic reticulum,and the Golgi apparatus,are involved in delivery with distinct participation *** importantly,the engagement of these organelles differentially affects the drug delivery efficiency,reflecting the spatial–temporal heterogeneity of nano delivery in *** summary,these findings highlight a significant methodological advance toward understanding the molecular mechanisms involved in the intracellular delivery of nanomedicines.

关键词： Enzyme-induced proximity labeling Intracellular delivery Nano-protein interaction Dynamic molecule profiling Macrophages

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Neutrophil programming dynamics and its disease relevance

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Science China(Life Sciences) 2017年第11期60卷 1168-1177页

作者： Taojing Ran shuo geng Liwu Li Department of Biological Sciences Virginia Polytechnic Institute and State UniversityBlacksburg VA24061USA Guangdong Provincial Key Laboratory of Gastroenterotogy Department of GastroenterologyNanfang HospitalSouthern Medicat UniversityGuangzhou 510515China

Neutrophils are traditionally considered as first responders to infection and provide antimicrobial host defense. However, recent advances indicate that neutrophils are also critically involved in the modulation of host immune environments by dynamically adopting distinct functional states. Functionally diverse neutrophil subsets are increasingly recognized as critical components mediating host pathophysiology. Despite its emerging significance, molecular mechanisms as well as functional relevance of dynamically programmed neutrophils remain to be better defined. The increasing complexity of neutrophil functions may require integrative studies that address programming dynamics of neutrophils and their pathophysiological relevance. This review aims to provide an update on the emerging topics of neutrophil programming dynamics as well as their functional relevance in diseases.

关键词： programming dynamics immune priming inflammatory diseases

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Degradation and mineralization of aniline by O_3/Fenton process enhanced using high-gravity technology

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Chinese Journal of Chemical Engineering 2018年第7期26卷 1444-1450页

作者： Yuejiao Qin Shuai Luo shuo geng Weizhou Jiao Youzhi Liu Shanxi Province Key Laboratory of Higee-Oriented Chemical Engineering North University of China Department of Civil and Environmental Engineering Virginia Polytechnic Institute and State University

The degradation and mineralization of aniline （AN） using ozone combined with Fenton reagent （O3/Fenton） in a rotating packed bed （RPB） was proposed in this study, and the process （RPB-O3/Fenton） was compared with conventional O3/Fenton in a stirred tank reactor （STR-O3/Fenton） or single ozonation in an RPB （RPB-O3）, Effects of high gravity factor, H2O2 dosage, H2O2 dosing method and initial pH on the AN mineralization efficiency were investigated in the RPB-O3/Fenton process, In addition, the behavior of Fe（Ⅱ） was monitored at different H2O2 dosing methods and pH values. Finally, the optimal operation conditions were determined with high gravity factor of 100, initial pH of 5, Fe（Ⅱ） concentration of 0.8 mmol·L-1 and H2O2 dosage of 2.5 ml. Under these conditions, for aniline wastewater at the volume of I L and concentration of 200 mg· L- 1 ,a fast and thorough decay of AN was conducted in 10 min, and the TOC removal efficiency reached 89% in 60 min. The main intermediates of p-benzoquinone, nitrobenzene, maleic acid and oxalic acid were identified by liquid chromatography/mass spectroscopy （LC/MS）, and the degradation pathways of AN in RPB-O3/Fenton system were proposed based on experimental evidence. It could be envisioned that high-gravity technology combined with O3/Fenton processes would be promising in the rapid and efficient mineralization ofwastewater.

关键词： O3/Fenton process High-gravity technology Aniline Degradation mechanisms

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圈养马麝的攻击性与粪样类固醇激素水平及麝香分泌的关系

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兽类学报 2024年第3期44卷 344-350页

作者：王晓龙吴佳忆周鑫吕青昕申立泉耿硕孟秀祥中国人民大学生态环境学院北京100872 西藏大学生态环境学院拉萨850000

动物行为的发育和表达受激素调控,动物的攻击性等个性特征与其激素水平关系紧密,并影响其生长和繁殖。本研究于2021年10月2日至12月15日采用焦点动物取样法和全事件记录法对兴隆山马麝繁育中心53头圈养雄性马麝(Moschus chrysogaster)... 详细信息

动物行为的发育和表达受激素调控,动物的攻击性等个性特征与其激素水平关系紧密,并影响其生长和繁殖。本研究于2021年10月2日至12月15日采用焦点动物取样法和全事件记录法对兴隆山马麝繁育中心53头圈养雄性马麝(Moschus chrysogaster)进行行为取样,结合粪样类固醇激素水平检测,分析雄麝的攻击性与粪样皮质醇水平、睾酮水平及麝香分泌的关系。结果表明:马麝的攻击性与粪样皮质醇水平呈显著正相关(r=0.311,P=0.023),攻击性强的马麝个体皮质醇水平(60.41±2.82)ng/g显著高于攻击性弱的个体(49.35±4.32)ng/g;攻击性与粪样睾酮水平呈显著正相关(r=0.326,P=0.017),攻击性强的马麝个体睾酮水平(101.27±4.94)ng/g显著高于攻击性弱的个体(84.48±4.10)ng/g;攻击性与麝香分泌量之间呈显著负相关(r=−0.282,P=0.041),攻击性弱的马麝个体麝香分泌量(10.62±0.58)g显著高于攻击性强的个体(8.28±0.91)g;攻击性与麝香酮含量之间呈显著负相关(r=−0.285,P=0.039),攻击性弱的马麝个体麝香酮含量(1.98±0.18)%显著高于攻击性强的个体(1.31±0.23)%。基于本研究结果,在麝类驯养实践中,建群时要避免较强攻击性个体同处一群,并基于攻击性的快速评估及粪样激素的实时监测等,即时优化圈养麝的社群结构,减少麝群的社群胁迫,提升个体的麝香分泌。本研究结果可为麝类迁地保育的优化及药用麝香的可持续生产提供参考。

关键词：马麝攻击性粪样类固醇激素麝香分泌麝香酮

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Engineering defects and adjusting electronic structure on S doped MoO2 nanosheets toward highly active hydrogen evolution reaction

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Nano Research 2020年第1期13卷 121-126页

作者： shuo geng Yequn Liu Yong Sheng Yu Weiwei Yang Haibo Li Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education Jilin Normal UniversityChangchun130103China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin150001China Analytical Instrumentation Center State Key Laboratory of Coal ConversionInstitute of Coal ChemistryChinese Academy of SciencesTaiyuan030001China

The electrocatalytic hydrogen evolution reaction(HER)is one of the most promising ways for low-cost hydrogen production in the *** this work,hetero S atoms were introduced into the MoO2 to enhance the catalytic activity by simultaneously adjusting electron structure,engineering lattice defect,and increasing oxygen *** the S doped MoO2 nanosheets with proper S doping amount show the enhanced performance for *** optimized catalyst shows a small onset overpotential as low as 120 mV,a low overpotential of 176 mV at the current density of 10 mA/cm^2 which is decreased 166 mV compared to that of the pristine MoO2 nanosheets,a low Tafel slope of 57 mV/decade,and a high turnover frequency of 0.13 H2/s per active site at 150 *** finding proposes an effective strategy to prepare nonprecious metal oxide catalyst for enhancing HER performance by rationally doping hetero atoms.

关键词： S doping MoO2 defect electron structure charge transfer

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均匀化处理对激光选区熔化GH3536和GH4169合金组织和显微硬度的影响

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航空材料学报 2024年第1期44卷 72-83页

作者：耿硕张冬云李健民仪登豪池煜璟黄帅张学军北京工业大学物理与光电工程学院北京100124 北京市数字化医疗3D打印工程技术研究中心北京100124 中国航发北京航空材料研究院北京100095

GH3536和GH4169镍基高温合金常用来制造航空发动机等热端部件。采用激光选区熔化(selective laser melting,SLM)最优工艺参数制备GH3536和GH4169合金试样,研究不同均匀化温度和保温时间对两种合金组织演变、平均晶粒尺寸和性能的影响,利... 详细信息

GH3536和GH4169镍基高温合金常用来制造航空发动机等热端部件。采用激光选区熔化(selective laser melting,SLM)最优工艺参数制备GH3536和GH4169合金试样,研究不同均匀化温度和保温时间对两种合金组织演变、平均晶粒尺寸和性能的影响,利用OM、SEM、EDS等方法表征其缺陷特征和微观组织,利用维氏硬度仪测试合金的显微硬度。结果表明:成形态GH3536合金中存在更多缺陷,包括气孔、裂纹和未熔合;成形态GH4169合金中只存在气孔。均匀化处理使合金熔池消失,使晶粒长大成为等轴晶。GH3536合金晶界和晶内有M_(23)C_(6)析出,GH4169合金晶界和晶内有NbC析出,随着均匀化温度升高,析出物明显减少。GH3536合金的平均晶粒尺寸从1130℃,1 h的48.5μm增大到1250℃,4 h的100.9μm,增大了106.8%;GH4169的平均晶粒尺寸从1080℃,1 h时的57μm增大到1200℃,4 h时的87.4μm,增加了53.3%。GH3536合金经过均匀化处理后显微硬度下降明显,由原来262HV下降到180~190HV;与之相反,GH4169合金经过均匀化处理后硬度提高,显微硬度均保持在430~450HV之间,明显高于成形态试样的313HV。

关键词：激光选区熔化 GH3536 GH4169 微观组织均匀化热处理平均晶粒尺寸显微硬度

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Activating interfacial S sites of MoS_(2) boosts hydrogen evolution electrocatalysis

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Nano Research 2022年第3期15卷 1809-1816页

作者： shuo geng Fenyang Tian Menggang Li Yequn Liu Jie Sheng Weiwei Yang Yongsheng Yu Yanglong Hou Laboratory for Space Environment and Physical Science Research Center of Basic Space ScienceHarbin Institute of TechnologyHarbin 150001China MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001China Analytical Instrumentation Center State Key Laboratory of Coal ConversionInstitute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China Beijing Key Laboratory for Magnetoelectric Materials and Devices(BKL-MMD) Beijing Innovation Center for Engineering Science and Advanced Technology(BIC-ESAT)School of Materials Science and EngineeringPeking UniversityBeijing 100871China

The hydrogen evolution reaction(HER)of molybdenum disulfide(MoS_(2))is limited in alkaline and acid solution because the active sites are on the finite edge with extended basal plane remaining ***,we activated the interfacial S sites by coupling with Ru nanoparticles on the inert basal plane of MoS_(2)*** density functional theory(DFT)calculation and experimental results show that the interfacial S electronic structure was *** the results of∆G H*demonstrate that the adsorption of H on the MoS_(2)was also *** the advantage of interfacial S sites activation,the Ru-MoS_(2)needs only overpotential of 110 and 98 mV to achieve 10 mA·cm^(–2)in both 0.5 M H_(2)SO_(4)and ^(1) M KOH solution,*** strategy paves a new way for activating the basal plane of other transition metal sulfide electrocatalysts for improving the HER performance.

关键词： electronic structure interfacial S sites d-band center hydrogen evolution reaction

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Ti-6Al-4V-5Cu synthesized for antibacterial effect in vitro and in vivo via contact sterilization

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Journal of Materials Science & Technology 2021年第31期90卷 133-142页

作者： Duo Xu Tianyu Wang Zhiyuan Lu Yuanqi Wang Bin Sun Shudan Wang Qiang Fu Zhenggang Bi shuo geng Department of Orthopedics First Affliated Hospital of Harbin Medical UniversityHarbin 150001Chinal Department of Cardiology First Affliated Hospital of Harbin Medical UniversityHarbin 150001China Center of Testing and Analysis College of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China Department of Ophthalmology First Affliated Hospital of Harbin Medical UniversityHarbin 150001China Department of General Surgery Beihai People’s HospitalBeihai 536000China

Implant-related infection is one of the most catastrophic complications after surgery,since it may trigger osteomyelitis and necessitate reoperation,increasing the pain suffered by and the burden placed on the *** a new alloy with an antibacterial effect for use as a biomedical material is an urgent *** this article,Ti-6 Al-4 V-5 Cu was synthesized under two different heat treatment schemes(solution temperatures of 850℃and 950℃).Through comprehensive observation of the microstructure,phase,copper ion release,biocompatibility,corrosion resistance and antibacterial effect in vitro and in vivo,it was determined that cell viability and corrosion resistance of Ti-6 Al-4 V-5 Cu was better than Ti-6 Al-4 *** importantly,the obtained alloy,especially samples treated at 850℃,showed prominent antibacterial ***,we also found that the main antibacterial mechanism of the alloy was contact sterilization via the extensive precipitation of Ti_(2)Cu instead of Cu ion release,and the content,surface area of Ti_(2)Cu could affect the antibacterial *** findings provide a very promising scheme by which to balance the biocompatibility and antibacterial effect of Ti-based,Cu-bearing alloys,which could improve their performance in orthopedic and dental applications.

关键词： Antibacterial effect Ti-based alloy Copper Ti_(2)Cu In vivo

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基于脑肠轴探究缺血性脑卒中与肠道菌群免疫的研究进展

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医学研究与教育 2024年第1期41卷 9-17页

作者：徐玉洁高娟王井辉任涵王子文耿硕承德医学院研究生学院河北承德067000 保定市第一中心医院神经内科河北保定071000 河北医科大学研究生学院河北石家庄050000 保定市第一中心医院内分泌实验室河北保定071000

人体肠道内定居着大量的微生物群,他们参与机体免疫稳态的调节,并与缺血性脑卒中的发生发展密切相关。脑肠轴的发现将肠道与大脑联系在一起,在人类的健康与疾病过程中发挥重要作用。现综述脑肠轴的调节机制、卒中后肠道菌群及其代谢物... 详细信息

人体肠道内定居着大量的微生物群,他们参与机体免疫稳态的调节,并与缺血性脑卒中的发生发展密切相关。脑肠轴的发现将肠道与大脑联系在一起,在人类的健康与疾病过程中发挥重要作用。现综述脑肠轴的调节机制、卒中后肠道菌群及其代谢物的调节以及基于脑肠轴对缺血性脑卒中后的大脑和肠道免疫反应的可能机制。这些研究为探索卒中后肠道和大脑免疫治疗的新方向和改善脑卒中预后提供了重要意义。

关键词：缺血性脑卒中肠道菌群脑肠轴免疫

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呼伦贝尔市典型黑土区土壤有机质与全氮含量关系研究

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中国资源综合利用 2024年第7期42卷 45-47页

作者：王璐孙秀琳姜英君吴耀义崔军冯丹耿朔呼伦贝尔市农牧技术推广中心内蒙古呼伦贝尔021008 呼伦贝尔农垦上库力农牧场有限公司内蒙古呼伦贝尔022257 内蒙古自治区海拉尔农牧场管理局内蒙古呼伦贝尔021008 莫力达瓦达斡尔族自治旗农村经营指导与产业服务中心内蒙古自治区莫力达瓦达斡尔族自治旗农业技术推广中心内蒙古莫力达瓦达斡尔族自治旗162891

为更好地保护黑土地,对呼伦贝尔市典型黑土区2238个土壤样品的有机质和全氮含量进行统计与线性回归分析。研究表明,土壤总氮与有机碳含量呈正相关,土壤有机质与土壤中总氮的线性回归关系存在较大差异。

关键词：土壤有机质全氮相关性黑土

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