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Infection-responsive long-term antibacterial bone plates for open fracture therapy

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Bioactive Materials 2023年第7期25卷 1-12页

作者： Lujiao Zhang Yurun Yang Yan-Hua Xiong Yu-Qing Zhao Zongpeng Xiu Hui-Min Ren Kai Zhang shun duan Ying Chen Fu-Jian Xu State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology)Ministry of EducationBeijing Laboratory of Biomedical MaterialsBeijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical TechnologyBeijing100029China Department of Orthopaedic Surgery China-Japan Friendship HospitalBeijing100029China

The infections in open fracture induce high morbidity worldwide.Thus,developing efficient anti-infective orthopedic devices is of great significance.In this work,we designed a kind of infection-responsive long-term antibacterial bone plates.Through a facile and flexible volatilization method,a multi-aldehyde polysaccharide derivative,oxidized sodium alginate,was crosslinked with multi-amino compounds,gentamycin and gelatin,to fabricate a uniform coating on Ti bone plates via Schiff base reaction,which was followed by a secondary crosslinking process by glutaraldehyde.The double-crosslinked coating was stable under normal condition,and could responsively release gentamycin by the triggering of the acidic microenvironment caused by bacterial metabolism,owning to the pH-responsiveness of imine structure.The thickness of the coating was ranging from 22.0μm to 63.6μm.The coated bone plates(Ti-GOGs)showed infection-triggered antibacterial properties(>99%)and high biocompatibility.After being soaked for five months,it still possessed efficient antibacterial ability,showing its sustainable antibacterial performance.The in vivo anti-infection ability was demonstrated by an animal model of infection after fracture fixation(IAFF).At the early stage of IAFF,Ti-GOGs could inhibit the bacterial infection(>99%).Subsequently,Ti-GOGs could promote recovery of fracture of IAFF.This work provides a convenient and universal strategy for fabrication of various antibacterial orthopedic devices,which is promising to prevent and treat IAFF.

关键词： Antibacterial Open fracture Infection-responsive Coating Orthopedic medical device

Reduction-responsive nucleic acid nanocarrier-mediated miR-22 inhibition of PI3K/AKT pathway for the treatment of patient-derived tumor xenograft osteosarcoma

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Bioactive Materials 2023年第10期28卷 376-385页

作者： Dafu Chen Chengyue Lei Weifeng Liu Meiyu Shao Meizhou Sun Jianxun Guo Jingjing Cao Jing-Jun Nie Peng Luo Yuwen Luo Bingran Yu Renxian Wang shun duan Fu-Jian Xu Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsNational Center for OrthopaedicsBeijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology)Ministry of EducationBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China Department of Orthopaedic Oncology Surgery Beijing Jishuitan HospitalPeking UniversityBeijing100035China JST Sarcopenia Research Centre Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan HospitalBeijing100035China

miRNAs are important regulators of gene expression and play key roles in the development of cancer, including osteosarcoma. During the development of osteosarcoma, the expression of miR-22 is significantly downregulated, making miR-22 as a promising therapeutic target against osteosarcoma. To design and fabricate efficient delivery carriers of miR-22 into osteosarcoma cells, a hydroxyl-rich reduction-responsive cationic polymeric nanoparticle, TGIC-CA (TC), was developed in this work, which also enhanced the therapeutic effects of Volasertib on osteosarcoma. TC was prepared by the ring-opening reaction between amino and epoxy groups by one-pot method, which had the good complexing ability with nucleic acids, reduction-responsive degradability and gene transfection performance. TC/miR-22 combined with volasertib could inhibit proliferation, migration and promote apoptosis of osteosarcoma cells in vitro. The anti-tumor mechanisms were revealed as TC/ miR-22 and volasertib could inhibit the PI3K/Akt signaling pathway synergistically. Furthermore, this strategy showed outstanding tumor suppression performance in animal models of orthotopic osteosarcoma, especially in patient-derived chemo-resistant and chemo-intolerant patient-derived xenograft (PDX) models, which reduced the risk of tumor lung metastasis and overcame drug resistance. Therefore, it has great potential for efficient treatment of metastasis and drug resistance of osteosarcoma by the strategy of localized, sustained delivery of miR-22 using the cationic nanocarriers combined with non-traditional chemotherapy drugs.

关键词： Nucleic acid delivery Nanoparticle Gene therapy Osteosarcoma Responsive Patient-Derived Xenograft(PDX)

不排水循环荷载下纤维加固砂土的超孔压及流动液化特性研究

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岩土力学 2024年第2期45卷 465-476页

作者：张希栋董晓强段伟胡顺磊张豪儒太原理工大学土木工程学院山西太原030024 太原理工大学山西省土木工程防灾与控制重点实验室山西太原030024

通过对纤维加固超疏松和疏松饱和砂土开展不排水动三轴试验,分析了其超孔压发展规律和流动液化特性。结果表明,未加固超疏松和疏松饱和砂土具有较高的液化势,其在不排水循环荷载下均发生流动液化。离散纤维向砂土骨架提供加密效应和约... 详细信息

通过对纤维加固超疏松和疏松饱和砂土开展不排水动三轴试验,分析了其超孔压发展规律和流动液化特性。结果表明,未加固超疏松和疏松饱和砂土具有较高的液化势,其在不排水循环荷载下均发生流动液化。离散纤维向砂土骨架提供加密效应和约束效应,从而提高其抗流动液化特性,然而,纤维的约束效应受到加载路径和试样应变发展模式的显著影响。纤维加固改变了饱和砂土残余孔压的发展规律,当纤维向砂土骨架施加较强的约束效应时,试样的残余孔压发展呈倒L型,明显不同于未加固砂土的S型发展。在双向非对称和单向循环荷载下,纤维应力贡献较大,砂土骨架的有效应力在超孔压上升100%后远大于0,加固试样的强度损失低于11%,纤维阻止了液化的发生。

关键词：纤维加固砂土流动液化超孔压强度损失有效应力

Polycaprolactone/polysaccharide functional composites for lowtemperature fused deposition modelling

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Bioactive Materials 2020年第2期5卷 185-191页

作者： Yu-Qing Zhao Ji-Hao Yang Xiaokang Ding Xuejia Ding shun duan Fu-Jian Xu State Key Laboratory of Chemical Resource Engineering Key Lab of Biomedical Materials of Natural Macromolecules(Beijing University of Chemical Technology)Ministry of EducationBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China

Fused deposition modelling(FDM)is a commonly used 3D printing technology.The development of FDM materials was essential for the product quality of FDM.In this work,a series of polycaprolactone(PCL)-based composites for low-temperature FDM were developed.By melt blending technique,different ratios of starch were added into PCL to improve the performances of FDM,and the printability,tensile strength,rheological properties,crystallization behaviors and biological performances of the composites were studied.The PCL/starch composite had the best performance in FDM process with the starch ratio of 9 ph at 80–90°C.The melting strength and solidification rate of PCL/starch composites were improved.The starch also increased the crystallization temperature,degree of crystallinity and crystallization rate of PCL/starch composites,while had no negative effects on the tensile strength of PCL.Due to the low printing temperature,various kinds of bioactive components were added into PCL/starch composites for preparation of antibacterial and biocompatible materials for FDM.The present work provides a new method to develop novel low-temperature FDM materials with various functions.

关键词： Fused deposition modelling Melt blending Polycaprolactone Composite Antibacterial

福建长汀汀江国家湿地公园维管植物的组成和地理成分

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湿地科学 2024年第1期22卷 88-97页

作者：黄磊张秀华郭华段萍郭舜闽西职业技术学院福建龙岩364021

2022年6月至11月期间,在福建长汀汀江国家湿地公园中,采用调查样线方法和重点普查方法,开展了维管植物野外调查,建立了维管植物名录,分析了维管植物的组成和地理成分。研究结果表明,调查期间,在该湿地公园中记录了114科299属424种维管植... 详细信息

2022年6月至11月期间,在福建长汀汀江国家湿地公园中,采用调查样线方法和重点普查方法,开展了维管植物野外调查,建立了维管植物名录,分析了维管植物的组成和地理成分。研究结果表明,调查期间,在该湿地公园中记录了114科299属424种维管植物,其中,有蕨类植物15科24属32种,有裸子植物5科5属5种,有被子植物94科270属387种;禾本科(Poaceae)、菊科(Asteraceae)、莎草科(Cyperaceae)和豆科(Fabaceae)为优势科,分别包含34属48种、22属33种、11属22种和15属20种维管植物,优势属不明显;维管植物的科和属的区系都既有明显的热带属性,又有一些温带成分;维管植物的区系丰富程度和种系分化程度都较低,维管植物的生存环境相对简单。

关键词：维管植物组成地理成分福建长汀汀江国家湿地公园

长白山区发酵酱菜中高产胞外多糖乳酸菌的筛选及多糖抗氧化性分析

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食品工业科技 2024年第2期45卷 110-117页

作者：尉洁张玲芳胡顺安秦孟春马琳李丹段翠翠长春大学农产品加工吉林省普通高等学校重点实验室吉林长春130022

本研究旨在从发酵酱菜中筛选高产胞外多糖的乳酸菌,并探究其多糖的抗氧化性,以期寻找一种天然来源的、安全的抗氧化剂。从吉林省延吉市采集的300余份特色发酵酱菜中分离出160株乳酸菌(lactic acid bacteria,LAB),根据菌落产粘法筛选出1... 详细信息

本研究旨在从发酵酱菜中筛选高产胞外多糖的乳酸菌,并探究其多糖的抗氧化性,以期寻找一种天然来源的、安全的抗氧化剂。从吉林省延吉市采集的300余份特色发酵酱菜中分离出160株乳酸菌(lactic acid bacteria,LAB),根据菌落产粘法筛选出10株产胞外多糖(Exopolysaccharides,EPS)的乳酸菌,进一步以胞外多糖产量较高的菌株A63、A120、A128作为目的菌株提取粗多糖,分离提取出的EPS产量分别为0.806、0.663和0.630 g/L,经16S rDNA和pheS基因序列比对分析鉴定三株乳酸菌均为植物乳植杆菌。利用DPPH自由基、ABTS+自由基和OH自由基清除率为指标评估胞外多糖的体外抗氧化活性。结果表明,三株植物乳植杆菌的胞外多糖都具有一定的自由基清除能力,其中菌株A63所产胞外多糖浓度为10 mg/mL时,对DPPH、ABTS+和OH三种自由基的清除率分别为90.47%、51.67%和35.08%,显著高于同浓度下其他菌株胞外多糖的抗氧化能力(P<0.05),可以作为天然抗氧化剂的潜在来源。

关键词：发酵酱菜乳酸菌胞外多糖分离提取抗氧化性

肺腺癌合并血管内大B细胞淋巴瘤1例并文献复习

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中国肺癌杂志 2024年第2期27卷 152-156页

作者：王彤彤陈小悦段国辰张霄鹏赵庆涛许顺赵焕芬河北医科大学研究生学院石家庄050000 河北省人民医院病理科河北省儿童医院胸外科河北省人民医院胸外科中国医科大学附属第一医院胸外科沈阳110001

血管内大B细胞淋巴瘤(intravascular large B-cell lymphoma,IVLBCL)是一种侵袭性结外大B细胞淋巴瘤,在同一器官与其他恶性肿瘤同时发生非常罕见,尤其是肺。本文报道1例肺腺癌合并IVLBCL的罕见病例。患者因腹泻伴发热、咳嗽入院。胸部... 详细信息

血管内大B细胞淋巴瘤(intravascular large B-cell lymphoma,IVLBCL)是一种侵袭性结外大B细胞淋巴瘤,在同一器官与其他恶性肿瘤同时发生非常罕见,尤其是肺。本文报道1例肺腺癌合并IVLBCL的罕见病例。患者因腹泻伴发热、咳嗽入院。胸部计算机断层扫描(computed tomography,CT)示右肺上叶不规则斑片状高密度影,边缘有磨玻璃影。入院后给予患者抗感染等治疗,但仍有间断低热(最高37.5 ℃)。经皮肺穿刺活检(percutaneous lung biopsy,PLB)病理诊断为贴壁生长为主的腺癌,局部考虑浸润。手术后病理诊断为肺浸润性非黏液性腺癌合并IVLBCL。本文通过分析其临床病理特征,并复习相关文献,以提高临床和病理医师对该肿瘤的认识,避免漏诊或误诊。

关键词：肺肿瘤肺腺癌血管内大B细胞淋巴瘤

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基于双流特征互补的嵌套命名实体识别

计算机工程与设计 2024年第3期45卷 799-805页

作者：黄荣梅廖涛张顺香段松松安徽理工大学计算机科学与工程学院安徽淮南232001

针对以往句子在文本编码后不能获得高效的特征信息,提出一种基于双流特征互补的嵌套命名实体识别模型。句子在嵌入时以单词的字级别和字符级别两种方式嵌入,分别通过神经网络Bi-LSTM获取句子上下文信息,两个向量进入低层级与高层级的特... 详细信息

针对以往句子在文本编码后不能获得高效的特征信息,提出一种基于双流特征互补的嵌套命名实体识别模型。句子在嵌入时以单词的字级别和字符级别两种方式嵌入,分别通过神经网络Bi-LSTM获取句子上下文信息,两个向量进入低层级与高层级的特征互补模块,实体词识别模块和细粒度划分模块对实体词区间进行细粒度划分,获取内部实体。实验结果表明,模型相较于经典模型在特征提取上有较大的提升。

关键词：命名实体识别自然语言处理嵌套结构双流特征互补神经网络实体词识别细粒度划分

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多维异质异构大型构件智能增材制造研究进展

科学通报 2024年第17期69卷 2401-2416页

作者：王克鸿彭勇段梦伟章晓勇黄勇贺申陈振文郭顺李晓鹏南京理工大学材料科学与工程学院南京210094 南京理工大学受控电弧智能增材技术工信部重点实验室南京210094 特种车辆设计制造集成技术全国重点实验室包头014030

电弧增材是近年发展起来的一种高效率、低成本、高性能、低精度整体制造方法,可成形超高强钢、轻合金等多种金属构成的一体化高性能构件.电弧-激光复合、增材-形变、增材-减材等复合成形技术,可进一步提高成形精度,提升构件韧性,更好地... 详细信息

电弧增材是近年发展起来的一种高效率、低成本、高性能、低精度整体制造方法,可成形超高强钢、轻合金等多种金属构成的一体化高性能构件.电弧-激光复合、增材-形变、增材-减材等复合成形技术,可进一步提高成形精度,提升构件韧性,更好地成形异质异构构件.本文从多维异质异构概念内涵、电弧复合增材技术、电弧增材过程智能控制等方面对多维异质异构大型构件智能电弧增材技术进行了综述,重点分析了增材过程参数-熔池视觉-应力-变形等协同传感技术;利用深度学习等人工智能方法,在线调整工艺参数,控制缺陷、抑制应力、减小变形,研制的大型多维异质构件多机器人智能复合增材装备,最大可增材10 m×4 m×4 m多金属构件;分析了电弧增材构件微观组织演变、静(动)态力学性能和抗超高速冲击性能特征;最后,指出了多维异质异构增材技术的4大发展趋势.

关键词：多维异质异构多维大型复杂构件受控电弧增材智能控制