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PI3 kinase isoform p110δis more important than p110αin KIT signaling in hematopoietic cells

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BIOCELL 2022年第9期46卷 2081-2087页

作者： LIANGYING ZHANG SHAOTING ZHANG ZHAOYANG FAN ZONGYING JIANG ANBU LIU shujing LI JIANMIN sun NHC Key Laboratory of Metabolic Cardiovascular Diseases Research Science and Technology CenterSchool of Basic Medical SciencesNingxia Medical UniversityYinchuan750004China General Hospital of Ningxia Medical University Yinchuan750004China

PI3 kinases are important for KIT signaling and KIT mutants mediated cell *** order to know the difference of PI3 kinase isoforms p110αand p110δin the signaling of wild-type KIT and the often occurred KIT mutation D816V in hematopoietic malignancy mastocytosis,the predominant PI3 kinase isoform p110δin hematopoietic tissues was knocked out in hematopoietic *** found that loss of p110δexpression dramatically inhibits PI3 kinase activation mediated by both wild-type KIT and KIT/*** over expression of p110αin p110δknock out cells,wild-type KIT mediated PI3 kinase activation was not changed while over expression of p110δincreased PI3 kinase ***,in KIT/D816V expressing cells without p110δexpression,over expression of p110δbut not p110αrestored PI3 kinase *** agreement with the signaling results,cell proliferation,cell survival and cell cycle assay further showed that over expression of p110δbut not p110αin p110δknock out cells increases both wild-type KIT and KIT/D816V mediated cell survival and *** results suggested that p110δplays a more important role than p110αin KIT signaling and KIT mutant mediated cell transformation in hematopoietic cells.

关键词： Mastocytosis Mutation Cell transformation

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用国产装置行主动脉内气囊反搏的实验研究

上海第一医学院学报 1982年第6期 455-460页

作者：杨西铭石美鑫任长裕王敏生孙蜀井上海市心血管病研究所上海第一医学院中山医院胸外科

本文总结了用国产装置对7头犬进行主动脉内气囊反搏的实验研究。通过反搏和停反搏的对比观察,表明用该装置进行反搏能提高冠状动脉的灌注压和血流量,改善受阻冠状动脉远端的侧支循环,并能减轻左室后负荷。其血液动力学效应与国外文献报... 详细信息

本文总结了用国产装置对7头犬进行主动脉内气囊反搏的实验研究。通过反搏和停反搏的对比观察,表明用该装置进行反搏能提高冠状动脉的灌注压和血流量,改善受阻冠状动脉远端的侧支循环,并能减轻左室后负荷。其血液动力学效应与国外文献报道相仿。同时证明该装置的安全性能良好。

关键词：主动脉内气囊反搏实验研究血流量收缩期回旋支左室后负荷冠状动脉装置

银耳二型态编码MAPK关键差异基因的克隆及生物信息学分析

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食药用菌 2024年第1期32卷 31-39页

作者：徐涛孔旭强占观平陈雅芳曹继璇李亚星孙淑静福建农林大学生命科学学院福建福州350002

银耳是我国特色的食用菌品种,其二型态转换与实际生产密切相关并制约着育种工作的进展,而丝裂原活化蛋白激酶(Mitogenactivatedproteinkinase,MAPK)途径在真菌二型态转换中具有重要调控作用。根据转录组测序数据克隆了银耳二型态编码MAP... 详细信息

银耳是我国特色的食用菌品种,其二型态转换与实际生产密切相关并制约着育种工作的进展,而丝裂原活化蛋白激酶(Mitogenactivatedproteinkinase,MAPK)途径在真菌二型态转换中具有重要调控作用。根据转录组测序数据克隆了银耳二型态编码MAPK的关键差异基因,包括TfFus3基因、TfSlt2基因和Kss1基因。分析表明,TfFus3、TfSlt2、Kss1基因的基因组DNA序列全长分别为1721 bp、1865 bp、1903 bp,ORF序列全长分别为1191 bp、1281 bp、1149 bp,分别含有10、12、16个内含子,编码396、426、382个氨基酸,相对分子质量在43.61 kDa~48.15 kDa间,理论等电点在5.82~6.89。序列同源性分析表明,TfFus3、TfSlt2、Kss1基因所编码的氨基酸序列分别与Kwoniella dejecticola、***、Tremalla mesenterica中同源蛋白的相似性最高。结构域分析表明,TfFus3、TfSlt2、Kss1蛋白都具有典型的蛋白激酶保守结构域且同属于PKc_likesuper family。本研究结果为进一步探究MAPK信号通路在银耳二型态转换中的作用奠定了基础。

关键词：银耳丝裂原活化蛋白激酶二型态生物信息学

Cloud Microphysical Processes and Atmospheric Water Budget during the 20 July 2021 Extreme Precipitation Event in Zhengzhou,China

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Journal of Meteorological Research 2023年第5期37卷 722-742页

作者： Weixi SHU Danhong FU Hui XIAO Huiling YANG Yue sun Xueliang GUO Yang ZHAO Jianfang DING shujing SHEN Key Laboratory of Cloud–Precipitation Physics and Severe Storms(LACS) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029 School of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049 School of Earth and Space Sciences University of Science and Technology of ChinaHefei 230026 Weather Modification Center of Henan Province Zhengzhou 450003 CMA Cloud–Precipitation Physics and Weather Modification Key Laboratory Weather Modification CentreChina Meteorological Administration(CMA)Beijing 100081

This study investigated the cloud microphysical processes and atmospheric water budget during the extreme precipitation event on 20 July 2021 in Zhengzhou of Henan Province,China,based on observations,reanalysis data,and the results from the high-resolution large-eddy simulation nested in the Weather Research and Forecasting(WRF)model with assimilation of satellite and radar *** results show that the abundant and persistent southeasterly supply of water vapor,induced by Typhoons In-Fa and Cempaka,under a particular synoptic pattern featured with abnormal northwestward displacement of the western Pacific subtropical high,was conducive to warm rain processes through a high vapor condensation rate of cloud water and an efficient collision–coalescence process of cloud water to *** conditions were favorable for the formation and maintenance of the quasi-stationary warmsector heavy *** formation through the collision–coalescence process of cloud water to rainwater accounted for approximately 70%of the total,while the melting of snow and graupel accounted for only approximately 30%,indicating that warm cloud processes played a dominant role in this extreme rainfall ***,enhancement of cold cloud processes promoted by latent heat release also exerted positive effect on rainfall during the period of most intense hourly *** was also found that rainwater advection from outside of Zhengzhou City played an important role in maintaining the extreme precipitation event.

关键词： extreme precipitation event microphysical processes atmospheric water budget large-eddy simulation

scRNA-seq profiling of neonatal and adult thymus-derived CD4+ T cells by a T cell origin-time tracing model

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Journal of Molecular Cell Biology 2022年第12期14卷 1-16页

作者： Yuheng Han Xinxing Ouyang Yao Chen shujing Lai Hongxiang sun Ningbo Wu Chun Ruan Limin Lu Bing Su Shanghai Institute of Immunology Department of Immunology and Microbiologyand the Ministry of Education Key Laboratory of Cell Death and DifferentiationShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Tumor Biology Shanghai Chest HospitalShanghai Jiao Tong University School of MedicineShanghai 200030China Center for Human Translational Immunology at Shanghai Institute of Immunology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai Jiao Tong University School of Medicine–Yale Institute for Immune Metabolism Shanghai Jiao Tong University School of MedicineShanghai 200025China Key Laboratory of Molecular Radiation Oncology of Hunan Province Xiangya HospitalCentral South UniversityChangsha 410008China

It is well documented that the neonatal thymus-derived (neonatal-TD) regulatory T cells (Treg) are essential to prevent lethal autoimmune diseases and allergies, and neonatal and adult thymus possesses distinct output potentials for naïve T cells, including Treg. However, the molecular features and detailed functional differences between neonatal-TD and adult thymus-derived (adult-TD) T cells in terms of their ability to maintain immune homeostasis during long-term environmental influences are still largely unknown, partially due to the lack of appropriate animal models to precisely trace these cells at specific time points. In this study, neonatal-TD and adult-TD CD4+ T cells from the spleen and Peyer's patches were traced for 9 weeks by a T cell origin-time tracing mouse model and analysed by single-cell RNA sequencing. More Treg but fewer naïve T cells were found in neonatal-TD CD4+ T cells from both tissues than those from adult-TD counterparts. Interestingly, the neonatal-TD Treg in both the spleen and Peyer's patches exhibited augmented expression of Foxp3, Gata3, Ctla4, Icos, Il2ra, Tgfb1, and Nrp1, as well as enriched Gene Ontology terms like T cell activation and tolerance induction, indicating an enhanced immunosuppressive function. These results were further confirmed by flow cytometry analysis and in vitro immune suppression assays. Flow cytometry also revealed a significantly higher proportion of neonatal-TD Treg in total Treg than that of adult-TD counterparts, suggesting the longer lifespan of neonatal-TD Treg. To investigate the intrinsic features of neonatal-TD and adult-TD CD4+ T cells, a shortened tracing time was performed. Surprisingly, the neonatal-TD and adult-TD CD4+ T cells had similar proportions of Treg and did not exhibit significant differences in Foxp3, Gata3, Ctla4, Icos, Il2ra, and Tgfb1 expression levels after tracing for 12 days. On the other hand, neonatal-TD Treg present an increased Nrp1 expression level compared with adult-TD co

关键词： T lineage tracing Treg neonatal thymus-derived T cells neonatal thymus-derived Treg single-cell RNA sequencing

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用注水浮囊导管测定健康狗左心室顺应值

上海第一医学院学报 1982年第4期 309-312页

作者：孙蜀井杨西铭任长裕王敏生钱肃凡上海第一医学院上海第一医学院中山医院胸外科上海市心血管病研究所研究生

改进了传统的液囊导管测定心室顺应值的方法,具有测定简便、准确性高和对心脏的刺激和损伤减轻等优点。评述了改进的浮囊导管的制作、测定步骤和注意事项。用该法对10条狗进行测定,提供了健康狗左心室顺应值曲线。

关键词：囊导管二尖瓣心脏浮囊舒张期僧帽瓣心瓣膜左心室瓣叶

SMIP-30,a potent and selective PPM1A inhibitor with potential to treat tuberculosis

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Acta Pharmaceutica Sinica B 2022年第12期12卷 4519-4521页

作者： shujing Xu Xinyong Liu Peng Zhan Department of Medicinal Chemistry Key Laboratory of Chemical BiologyMinistry of EducationSchool of Pharmaceutical SciencesShandong UniversityJi'nan 250012China

Recently,a collaborative research led by Weibo Yang and Jim sun1 published in Cell Chemical Biology identified a potent and selective small molecule inhibitor(SMIP-30)for human protein phosphatase Mg^(2+)/Mn^(2+)-dependent 1 A(PPM1A).They applied this chemical probe to determine the autophagy receptor p62 as a new substrate of PPM1A.

关键词： PPM1A Tuberculosis Drug discovery Compound library

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我国银耳产业发展现状与对策建议

食药用菌 2023年第6期31卷 349-354页

作者：孙淑静陈剑秋李佳欢曹继璇陈利丁黄亚东刘自强福建农林大学福建福州350002 中国食品土畜进出口商会食用菌分会北京100062

从产区分布、国内市场、出口贸易3个方面分析我国银耳产业发展现状,指出近年来我国银耳产量稳步提升。福建省作为银耳最大产区,近年产量占全国产量的80%以上。银耳国内市场总体向好,加工类产品中,冻干银耳羹、鲜炖银耳两类产品销量增长... 详细信息

从产区分布、国内市场、出口贸易3个方面分析我国银耳产业发展现状,指出近年来我国银耳产量稳步提升。福建省作为银耳最大产区,近年产量占全国产量的80%以上。银耳国内市场总体向好,加工类产品中,冻干银耳羹、鲜炖银耳两类产品销量增长。银耳出口数量和金额在2021年后回升,2022年我国银耳出口总量为4 566.09吨,同比增长18.47%;实现出口额8 813.18万美元,同比增长32.26%;出口单价19.30美元/千克,同比增长11.63%。出口贸易主要省份为福建和湖北,2022年实现出口额和全国出口总量的占比分别为5 566.41万美元、63.16%和2 208.58万美元、25.06%。泰国是我国银耳出口主要流向国,其次是中国香港地区。分析我国银耳产业在种业创新与保护、生产技术水平、精深加工等方面存在的问题,并提出对策建议。

关键词：银耳产业银耳产区贸易销售发展机遇

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物理杀虫矿粉防治茶树菇螨虫研究

食药用菌 2019年第6期27卷 390-394页

作者：林铃张琪辉金珊珊张凌姗李佳欢孙淑静胡开辉郑明锋古田县食用菌研发中心福建宁德352200 福建农林大学生命科学学院福建福州350002 福建农林大学食品科学学院福建福州350002

茶树菇病虫为害严重,首次探索物理杀虫矿粉(Celite610)防治茶树菇螨虫效果。结果显示,使用1.0%以上浓度的物理杀虫矿粉悬液防治螨虫,96 h后对螨虫有显著的毒性表现,粉末的重金属含量均明显低于限值,不会造成重金属残留;物理杀虫矿粉在8... 详细信息

茶树菇病虫为害严重,首次探索物理杀虫矿粉(Celite610)防治茶树菇螨虫效果。结果显示,使用1.0%以上浓度的物理杀虫矿粉悬液防治螨虫,96 h后对螨虫有显著的毒性表现,粉末的重金属含量均明显低于限值,不会造成重金属残留;物理杀虫矿粉在8%浓度以下对茶树菇菌丝生长速度、原基形成、子实体生长、初潮产量没有显著影响;喷洒2%的物理杀虫矿粉悬液可有效防治螨虫,并有助于刺激茶树菇的出菇。

关键词：物理杀虫矿粉茶树菇螨虫防治