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Effects of Load on Dry Sliding Wear Behavior of Mg–Gd–Zn–Zr Alloys

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Journal of Materials Science & Technology 2017年第7期33卷 645-651页

作者： Jie Zhang Xiaobo Zhang Qinghua Liu shujie Yang Zhangzhong wang School of Materials Science and Engineering Nanjing Institute of TechnologYNanjing 211167China Jiangsu-Key Laboratory of Advanced Structural Materials and APplication Technology Nanjing 211167China

Dry sliding wear tests on as-cast and T6-treated Mg-3Gd-1Zn-0.4Zr（wt%, GZ31K） and Mg-6Gd-1Zn-0.4Zr（wt%, GZ61K） alloys were performed using a ball-on-disk configuration at room temperature. Friction coefficient and wear rate of the alloys were measured under three different applied loads（50 N, 100 N, and 200 N, respectively）. Worn surface morphologies were analyzed using a scanning electron microscope（SEM） coupled with an energy dispersive spectrometer（EDS）. It is found that the friction coefficient of the alloys decreases with increasing load, except the as-cast GZ61 K. The wear rates of the as-cast Mg-Gd-Zn-Zr alloys increase with the increase of the load. However, the wear rates of the T6-treated Mg-Gd-Zn-Zr alloys first increase because of the participation of a large amount of needle-like precipitates, but then decline due to obvious work hardening. The wear mechanisms of abrasion, plastic deformation, oxidation, adhesion and delamination are detected. Abrasion dominates the wear mechanism under the low load; whereas, adhesion is the main wear mechanism under intermediate load, and plastic deformation has great effect on the wear rate under high applied load.

关键词： Magnesium alloys Applied load Wear and friction Wear mechanism

Effect of xanthan gum on the quality of low sodium salted beef and property of myofibril proteins

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Food Science and Human Wellness 2021年第1期10卷 112-118页

作者： Zirui Zhao shujie wang Dayu Li Yajun Zhou College of Biological and Agricultural Engineering Jilin UniversityChangchun130025China Department of Food Science and Engineering Jilin UniversityChangchun130062China

The effects of xanthan gum (0 % and 0.3 %) on the quality of low sodium (2.0 %, 2.4 %, 2.8 %, 3.2 %, 3.6 % and 4.0 %) salted beef and property of myofibril proteins (MP) extracted from salted beef were researched. Changes in the salting absorptivity, cooking loss, shear force, color and sensory analysis showed that xanthan gum had a positive effect on the quality of salted beef with low salinity (2.0 %, 2.4 % and 2.8 %). MP of salted beef treated with xanthan gum had higher solubility, lower hydrogen bond than that without it (P < 0.05). The decrease of surface hydrophobicity and intrinsic fluorescence intensity indicated that the chromophore of protein was brought into a hydrophobic environment in the presence of xanthan gum. The electrophoresis showed that xanthan gum could improve the concentration of myosin heavy chain, paramyosin and actin after tumble-salting. The data suggested that salted beef and its MP treated with xanthan gum and 2.8 % salt (XS3) had the best properties. Together, xanthan gum could be considered as a sodium salt substitute for the development of low sodium meat products.

关键词： Low sodium Myofibril proteins Salted beef Xanthan gum

Pharmacogenetic activation of midbrain dopaminergic neurons induces hyperactivity

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Neuroscience Bulletin 2013年第5期29卷 517-524页

作者： shujie wang Yan Tan Ju-En Zhang Minmin Luo First Hospital Jilin University National Institute of Biological Sciences The People's Hospital of Jilin Province School of Life Sciences Tsinghua University

Dopaminergic neurons regulate and organize numerous important behavioral processes including motor ***,manipulation of brain dopamine concentrations changes animal activity *** is synthesized by several neuronal populations in the *** study was carried out to directly test whether selective activation of dopamine neurons in the midbrain induces hyperactivity.A pharmacogenetic approach was used to activate midbrain dopamine neurons,and behavioral assays were conducted to determine the effects on mouse activity *** expression of the evolved hM3Dq receptor was achieved by infusing Creinducible AAV viral vectors into the midbrain of DATCre *** were excited by injecting the hM3Dq ligand clozapine-N-oxide（CNO）.Mouse locomotor activity was measured in an open *** results showed that CNO selectively activated midbrain dopaminergic neurons and induced hyperactivity in a dose-dependent manner,supporting the idea that these neurons play an important role in regulating motor activity.

关键词： pharmacogenetics electrophysiology motor activity clozapine-N-oxide

The selection and stability analysis of stable and high Taxol-producing cell lines from Taxus cuspidata

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Journal of Forestry Research 2018年第1期29卷 65-71页

作者： shujie wang Hujun wang Tong Li Chun Li Yajun Zhou Xiangmei Zhong College of Biological and Agricultural Engineering Jilin University Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University College of Food Science and Engineering Jilin University

In this study to screen for stable, high Taxolproducing cell lines（CL5, CL12, and CL21） of Taxus cuspidata, stem tissues were used to induce calli, which were then subcultured nine times to establish suspension cell cultures. From 97 cell lines obtained from conditioned cultures, 10 cell lines with high Taxol content were selected. Stability analyses on solid and liquid B5 media were then used to obtain lines that stably produced high levels of Taxol. Fresh biomass and Taxol production of the ninth generation became stable. Taxol content of selected CL5, CL12, and CL21 samples was 0.0448, 0.0477, and0.0428% of dry mass（DW）, respectively. Proliferation of CL5, CL12 and CL21 was 346.3, 382.5, and 409.2%,respectively. From work over about 2 years, the three cell lines appear suitable for mass production of Taxol,promoting the industrialisation and commercial-scale production of Taxol using cell culture.

关键词： Paclitaxel Taxus cuspidata Taxol Cell lines Stability analysis

Label-free selective sensing of Pb^(2+)lead(Ⅱ) sensors based on the aggregation of a pyrene fluorescent probe

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Chinese Science Bulletin 2014年第5期59卷 502-508页

作者： Yue Yang shujie wang Xinyu Hu Xiangyu Yang College of Biological and Agricultural Engineering Jilin University State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences

In the present work,we report a label-free fluorescence turn-on approach for the sensitive and selective sensing of Pb2?.Pyrene with one positive charge was used as the fluorescent probe,and thrombin aptamer(TBA),which was a G-rich oligonucleotide,was employed to form G-quadruplex with lead(II).When TBA and Pb2?were mixed with lead(II)in an aqueous solution,it was folded into a stable ***,a single-stranded nucleic acid-specific nuclease S1 was *** G-quadruplex stabilized by Pb2?lead(II)had markedly a significant resistant ability to nuclease S1 ***,in the absence of Pb2?lead(II),no quadruplex or less stable quadruplex was formed and TBA was digested by nuclease S1 in 3 min under the optimized experimental ***,pyrene probe was mixed with oligonucleotide in Pb2?lead(II).Electrostatic interactions between oligonucleotide(a polyanion)and the probe induced the aggregation of the probe,which in turn produced strong emission of the strong pyrene excimer *** intensity of the induced excimer emission was directly proportional to the amount of Pb2?*** approach shows good selectivity and sensitivity for the detection of Pb2?with a limit of detection limit as low as 800 nmol/L.

关键词：荧光探针无标记传感器聚集 G-四链体铅选择性检测芘

Geochemical Features of Sulfides from the Deyin-1 Hydrothermal Field at the Southern Mid-Atlantic Ridge near 15?S

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Journal of Ocean University of China 2017年第6期16卷 1043-1054页

作者： wang shujie LI Huaiming ZHAI Shikui YU Zenghui CAI Zongwei Key Laboratory of Submarine Geosciences and Prospecting Techniques Ministry of EducationCollege of Marine Geosciences Ocean University of China Qingdao 266100 P. R. China Key Laboratory of Submarine Geosciences Second Institute of Oceanography State Oceanic AdministrationHangzhou 310012 P. R. China

In this study, geochemical compositions of elements in sulfide samples collected from the Deyin-1 hydrothermal field near the 15?S southern Mid-Atlantic Ridge(SMAR) were analyzed by the X-ray fluorescence spectrometry(XRF) and inductively coupled plasma mass spectrometry(ICP-MS) to examine the enrichment regulations of ore-forming elements and hydrothermal mineralization. These sulfide precipitates can be classified macroscopically into three types: Fe-rich sulfide, Fe-Cu-rich sulfide and Fe-Zn-rich sulfide, and are characterized by the enrichment of base metal elements along with a sequence of Fe>Zn>Cu. Compared with sulfides from other hydrothermal fields on MAR, Zn concentrations of sulfides in the research area are significantly high, while Cu concentrations are relatively low. For all major, trace or rare-earth elements(REE), their concentrations and related characteristic parameters exhibit significant variations(up to one or two orders of magnitude), which indicates the sulfides from different hydrothermal vents or even a same station were formed at different stages of hydrothermal mineralization, and suggests the variations of chemical compositions of the hydrothermal fluid with respect to time. The hydrothermal temperatures of sulfides precipitation decreased gradually from station TVG10(***10) to ***12, and to ***11, indicating that the precipitation of hydrothermal sulfides is subjected to conditions changed from high temperature to low temperature, and that the hydrothermal activity of study area was at the late stage of a general trend of evolution from strong to weak. The abnormally low concentrations of REE in sulfides and their similar chondrite-normalized REE patterns show that REEs in all sulfides were derived from a same source, but underwent different processes of migration or enrichment, or sulfides were formed at different stages of hydrothermal mineralization. The sulfides collected from the active hydrothermal vent were mainly attrib

关键词： southern Mid-Atlantic Ridge Deyin-1 hydrothermal field hydrothermal sulfide geochemistry of element mineralization

Optimization of the ultrasonic-microwave assisted enzymatic hydrolysis of freshwater mussel meat

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International Journal of Agricultural and Biological Engineering 2018年第5期11卷 236-242页

作者： Yajun Zhou Xiaoli Yi Jingya wang Qi Yang shujie wang Department of Food Science and Engineering Jilin UniversityChangchun 130062China College of Biological and Agricultural Engineering Jilin UniversityChangchun 130025China

In the present study,the Ultrasonic-Microwave(US-MW)technique was applied for assisting enzymatic hydrolysis of freshwater mussel *** operating parameters of enzymatic hydrolysis were investigated and the properties and qualities of the freshwater mussel hydrolysates were also *** considerable optimum conditions for enzymatic hydrolysis of mussel meat were ratio of liquid to material of 10.36:1,power of 360.52 W,processing time of 6.91 min,enzymolysis time of 4 *** these conditions,a degree of hydrolysis(DH)of 48.40% was *** hydrolysates produced under optimum conditions,were characterized in terms of electrophoretic profile,amino acid composition and infrared spectrum ***-PAGE profiles of the hydrolysates revealed that several protein bands were cleaved and several light myosin chains(25 kDa and 15 kDa,respectively)and peptides of molecular weight lower than 6.5 kDa were *** infrared spectrum absorption peak of amide band A and band III shifted after US-MW pre-treatment,which indicated that the N-H(hydrogen bonding)stretching vibration and triple helix structure of samples were *** essential amino acids in hydrolysates with or without US-MW pre-treatment were 36.62 mg/100 g and 33.40 mg/100 *** was proved that US-MW assisted enzymatic hydrolysis is an effective technology to utilize freshwater mussel meat.

关键词： ultrasonic-microwave(US-MW) freshwater mussel meat enzymatic hydrolysis degree of hydrolysis(DH)

Solvothermal Synthesis of TiO2/Bi2WOs Heterojunction Photocatalyst with Optimized Interface Structure and Enabled Photocatalytic Performance

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Chinese Journal of Chemistry 2017年第2期35卷 183-188页

作者： Linpeng Jiang shujie wang Liyi Shi Yin Zhao Zhuyi wang Meihong Zhang Shuai Yuan Research Center of Nanoscience and Nanotechnology Shanghai University Shanghai 200444 China

TiO2/Bi2WO6 （TB） heterojunction photocatalyst was successfully synthesized and characterized by XRD, SEM, TEM, UV-Vis and photoluminescence measurement. The heterojunction interface structure of TB heterojunction photocatalyst was optimized via adjusting the ethylene glycol/water （EG/W） ratio. Based on XRD, SEM and TEM, the crystal size of Bi2WO6 reduced from 14.6 nm to 8.8 nm, and the interface structure between Bi2WO6 nanosheets and TiO2 particle significantly changed with increasing EG concentration. Furthermore, the photoeatalytic activity and the related mechanism of TB heterojunction photocatalyst were systematically discussed. Among them, TBEG/w sample shows the highest normalized apparent rate constant, which is attributed to its highest electron-hole pairs separation ability driven by optimized heterojunction interface between two semiconductors.

关键词： TiO2/Bi2WO6 heterojunction interface structure carriers separation photocatalytic

Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

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Journal of Forestry Research 2016年第6期27卷 1257-1263页

作者： shujie wang Chun Li Hujun wang Xiangmei Zhong Jing Zhao Yueying Tong Yajun Zhou College of Biological and Agricultural Engineering Jilin University Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University College of Food Science and Engineering Jilin University

In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultra- sonic extraction combined with TLC-UV spectrophotom- etry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin （GA3） were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

关键词： Elicitor Precursor Metabolic inhibitor Paclitaxel Cell culture Taxus cuspidata