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The CsMYB123 and CsbHLH111 are involved in drought stress‑induced anthocyanin biosynthesis in Chaenomeles speciosa

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Molecular Horticulture 2023年第1期3卷 51-63页

作者： Yanshen Ren shuangyu zhang Qianyi Zhao Yang Wu Houhua Li College of Landscape Architecture and Art Northwest A&F UniversityYangling 712100ShaanxiChina

Drought stress has been demonstrated to enhance the biosynthesis of anthocyanins in the leaves,resulting in an increased aesthetic ***,the molecular mechanisms underlying drought-induced anthocyanin biosynthesis in Chaenomeles speciosa remain *** this study,the metabolites of *** leaves were analyzed,and it was found that the content of cyanidin-3-O-rutinoside increased significantly under drought *** differentially expressed genes CsMYB123 and CsbHLH111 were isolated by transcriptomics data analysis and gene cloning,and gene overexpression and VIGS experiments verified that both play important roles in anthocyanin ***,Y1H and Dual-luciferase reporter assay showed that CsMYB123 binds to the promoters of anthocyanin biosynthesis-related structural genes(such as CsCHI,CsF3H,and CsANS),while CsbHLH111 was shown to bind to the promoter of CsCHI,positively regulating its ***,BIFC and Y2H assays unveiled potential protein–protein interactions between CsMYB123 and CsbHLH111 at the cell ***,these results shed light on the critical roles played by CsMYB123 and CsbHLH111 in anthocyanin biosynthesis,thus providing a valuable insight into understanding the molecular mechanisms of how the MYB and bHLH genes regulate anthocyanin biosynthesis in the process of leaf coloration in ***.

关键词： Chaenomeles speciosa Drought CsbHLH111 CsMYB123 Anthocyanin

Comparative analysis of four edible mushrooms based on HPLC fingerprint and pattein recognition analysis

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未来食品学报(英文) 2024年第1期4卷 105-110页

作者： Xiali Si Xinyu Liu shuangyu Shao Zhiyong Du Pengfei Tu Qingying zhang Strate Key Laboratory of Natoral oud Biomimenic Drugs and Deportment of Natral Menicines School of Phormocutical ScimncesPekang Uravensin Heath Science CenterBeijing 100191China

A high performance liquid chromatography-ultraviolet(HPLC-UV)fingerprint method for the overall chemical analysis of edible mushrooms was established based on Auricularia heimuer for the first time,and then applied to analyze 60 batches of ***,Auricularia *** cornea*Yu Muer’and TremeUa fuciformis.A total of 9 characteristic peaks of ***.11 characteristic peaks of ***,6 characteristic peaks of ***‘Yu Muer’,and 9 characteristic peaks of I fuciformis were ***,a combinatory analysis,including similarity evaluation,hierarchical cluster analysis and principal component analysis,revealed the chemical consistency and difference between samples from the same and different *** HPLC fingerprint method established in this paper could be used to characterize the components of ***,***,***‘Yu Muer’,and *** and discriminate the 4 edible mushrooms effectively in combination with pattern recognition analysis.

关键词： Edible mushrooms High performance liquid chromatography fingerprint Pattern recognition analysis Auricularia heimuer Auricularia cornea Tremella fiiciformis

Three rare anti-inflammatory sesquiterpene lactones from Magnolia grandiflora

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Chinese Journal of Natural Medicines 2024年第3期22卷 265-272页

作者： XU shuangyu zhang Feng TAO Linlan JIANG Yangming HUANG Tao LI Yanan HU Zhanxing YANG Jue HAO Xiaojiang YUAN Chunmao State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical UniversityGuiyang 550014China School of Pharmaceutical Sciences Guizhou Medical UniversityGuiyang 550025China Natural Products Research Center of Guizhou Province Guiyang 550014China State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China

Four new sesquiterpene lactones(SLs)(1–4),along with a biosynthetically related SL(5),have been isolated from the leaves of Magnolia *** A(1)is notable as the first C18 homogemarane type SL,featuring a unique 1,7-dioxaspiro[4.4]nonan-6-one *** 2 and 3,representing the first instances of chlorine-substituted gemarane-type SL analogs in natural products,were also *** structures of these isolates were elucidated through a combination of spectroscopic data analysis,electronic circular dichroism calculations,and X-ray single-crystal diffraction *** isolates demonstrated anti-inflammatory activity in lipopolysaccharide-stimulated RAW264.7 ***,3–5 showed a significant inhibitory effect on nitric oxide production,with IC50 values ranging from 0.79 to 4.73μmol·L^(−1).Additionally,4 and 5 exhibited moderate cytotoxic activities against three cancer cell lines,with IC50 values between 3.09 and 11.23μmol·L^(−1).

关键词： Magnolia grandiflora Sesquiterpene lactones Isolation and identification Anti-inflammatory activity

Formation and growth of nanophase iron particles on the surface of Mercury revealed by experimental study

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Acta Geochimica 2024年第4期43卷 774-784页

作者： Ronghua Pang Yang Li Chen Li Pengfei zhang Zhuang Guo Sizhe Zhao Han Yu Li Wang Chenxi Zhu shuangyu Wang Kairui Tai Qinwei zhang Yuanyun Wen Rui Li Center for Lunar and Planetary Sciences Institute of GeochemistryChinese Academy of SciencesGuiyangChina University of Chinese Academy of Sciences BeijingChina Center for Excellence in Comparative Planetology Chinese Academy of SciencesHefeiChina Institute of Remote Sensing and Geographical Information System School of Earth and Space SciencesPeking UniversityBeijingChina State Key Laboratory of Lunar and Planetary Sciences Macao University of Science and TechnologyMacaoChina Faculty of Metallurgical and Energy Engineering Kunming University of Science and TechnologyKunmingChina State Key Laboratory of High Performance Ceramics and Superfi ne Microstructures Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiChina Laboratory for Space Environment and Physical Sciences Harbin Institute of TechnologyHarbinChina Research Center for Planetary Science College of Earth ScienceChengdu University of TechnologyChengduChina Key Laboratory of Planetary Sciences Purple Mountain ObservatoryChinese Academy of SciencesNanjingChina

Space weathering is a primary factor in altering the composition and spectral characteristics of surface materials on airless ***,current research on space weathering focuses mainly on the Moon and certain types of *** particular,the impacts of meteoroids and micrometeoroids,radiation from solar wind/solar flares/cosmic rays,and thermal fatigue due to temperature variations are being *** weathering produces various transformation products such as melted glass,amorphous layers,iron particles,vesicles,and solar wind *** in turn lead to soil maturation,changes in visible and near-infrared reflectance spectra(weakening of characteristic absorption peaks,decreased reflectance,increased near-infrared slope),and alterations in magnetism(related to small iron particles),collectively termed the“lunar model”of space weathering *** to the Moon and asteroids,Mercury has unique spatial environmental characteristics,including more intense meteoroid impacts and solar thermal radiation,as well as a weaker particle radiation environment due to the global distribution of its magnetic ***,the lunar model of space weathering may not apply to *** studies have extensively explored the eff ects of micrometeoroid ***,this work focuses on the eff ects of solar-wind particle radiation in global magnetic-field distribution and on the weathering transformation of surface materials on Mercury under prolonged intense solar *** the utilization of highvalence state,heavy ion implantation,and vacuum heating simulation experiments,this paper primarily investigates the weathering transformation characteristics of the major mineral components such as anorthite,pyroxene,and olivine on Mercury’s surface and compares them to the weathering transformation model of the *** experimental results indicate that ion implantation at room temperature is insufficient to generate np-Fe^(0)directly but

关键词： Mercury Space weathering Np-Fe^(0) Solar thermal radiation Solar wind

微生物铁氧化还原作用对水中砷锑去除影响的研究进展

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化学进展 2022年第4期34卷 870-883页

作者：张双玉胡韵璇李成徐新华浙江大学环境与资源学院环境工程系杭州310058

砷锑污染在全球领域广泛存在,与常规的铁氧化物相比,微生物铁氧化生成的含Fe(Ⅲ)矿物对水中砷/锑(As/Sb)具有更强的吸附能力,并因其高效、实用和环境友好而具有广阔的应用前景,但微生物铁还原也可能导致被吸附的As/Sb再次释放。本文综... 详细信息

砷锑污染在全球领域广泛存在,与常规的铁氧化物相比,微生物铁氧化生成的含Fe(Ⅲ)矿物对水中砷/锑(As/Sb)具有更强的吸附能力,并因其高效、实用和环境友好而具有广阔的应用前景,但微生物铁还原也可能导致被吸附的As/Sb再次释放。本文综述了微生物铁氧化还原作用对As/Sb去除影响的研究进展,归纳了铁矿物“合成-溶解-转化”的微生物循环过程以及该循环伴随的水中As/Sb固定、溶解与转化机理,整合了微生物合成Fe(Ⅲ)矿物的矿物学性质、对As/Sb固定的热动力学规律和络合机制,总结了微生物合成Fe(Ⅲ)矿物对As/Sb去除的影响因素,基于该研究的现存问题展望了利用微生物铁氧化还原作用去除As/Sb的发展方向。

关键词：微生物铁矿物氧化还原除As/Sb

赤霉素对紫斑牡丹种子下胚轴萌发及其种皮和胚乳结构的影响

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西北植物学报 2019年第12期39卷 2187-2196页

作者：张双羽唐红何丽霞甘肃农业大学林学院兰州730070 甘肃省牡丹工程研究中心兰州730046

以紫斑牡丹种子为试验材料,考察不同浓度赤霉素(GA3)处理下种子生根情况,同时对其种皮纹饰进行了扫描电镜(SEM)观察,并对胚乳结构进行了荧光倒置显微镜、透射电镜(TEM)观察,以揭示GA3对紫斑牡丹种子下胚轴解除休眠过程中种子种皮、胚乳... 详细信息

以紫斑牡丹种子为试验材料,考察不同浓度赤霉素(GA3)处理下种子生根情况,同时对其种皮纹饰进行了扫描电镜(SEM)观察,并对胚乳结构进行了荧光倒置显微镜、透射电镜(TEM)观察,以揭示GA3对紫斑牡丹种子下胚轴解除休眠过程中种子种皮、胚乳结构变化和生根的影响。结果表明:(1)GA3处理能够促进紫斑牡丹种子生根,以300 mg/L GA3处理的种子生根效果最好,与对照相比可提前14.66 d生根,生根率可达62.33%。(2)扫描电镜观察发现,紫斑牡丹种皮表面含有蜡质,种子萌发进程中蜡质明显不断增加,种皮表面网眼逐渐消失,种皮皱缩明显。(3)光学显微镜观察发现,GA3处理的紫斑牡丹种子胚乳细胞中含有较多淀粉体和蛋白体,并随着种子萌发进程逐渐积累,可为种子萌发提供足够的养分。(4)透射电镜观察发现,GA3处理的紫斑牡丹种子胚乳细胞内大分子营养物质不断降解,线粒体、内质网、高尔基体等细胞器相继出现,种子下胚轴休眠解除,细胞壁较薄后期消融,利于营养物质传送。研究认为,适宜浓度GA3处理可有效加快紫斑牡丹种皮结构的改变和胚乳细胞内物质代谢进程,促进种子萌发,且300 mg/L GA3处理紫斑牡丹种子24 h可以获得最好的生根效果。

关键词：紫斑牡丹赤霉素种子生根种皮纹饰胚乳细胞结构

图书馆开办道德讲堂的实践与思考——以哈尔滨市图书馆讲座工作为例

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科技情报开发与经济 2013年第17期23卷 90-92页

作者：张双羽哈尔滨市图书馆黑龙江哈尔滨150086

以哈尔滨市图书馆讲座工作为例,对图书馆开办道德讲堂的实践,道德讲堂的定位、现实意义及应重点解决的问题进行了思考。

关键词：图书馆讲座道德讲堂哈尔滨市图书馆

Multisim辅助的乳化液泵站智能控制系统本安电源双重保护电路设计

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同煤科技 2019年第4期 24-26,29页

作者：张霜玉天地科技股份有限公司中煤科工集团国际工程有限公司

本文利用Multisim软件对综采液压支架的动力源乳化液泵站智能控制系统的本质安全型电源电路进行辅助设计,并设计了双重稳压电路和双重过压、过流保护电路。该本安电源经Multisim软件对输出电压、电流效果进行仿真后发现供电电压会出现... 详细信息

本文利用Multisim软件对综采液压支架的动力源乳化液泵站智能控制系统的本质安全型电源电路进行辅助设计,并设计了双重稳压电路和双重过压、过流保护电路。该本安电源经Multisim软件对输出电压、电流效果进行仿真后发现供电电压会出现瞬间增大现象,根据该现象对电路进行了局部修改,修改后供电电压得到了明显改善。

关键词： Multisim 本安电源电路设计

外源GA_(3)对紫斑牡丹幼苗叶片解剖结构与光合特性及内源激素含量的影响

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西北植物学报 2021年第3期41卷 450-460页

作者：张瑞张双羽唐红李婉茹何丽霞甘肃农业大学林学院兰州730070 甘肃省牡丹工程研究中心兰州730046

以1年生紫斑牡丹幼苗为试验材料,采用不同浓度(0、100、300、500 mg/L)赤霉素(GA_(3))喷施叶片处理,通过透射电镜、扫描电镜、光学显微镜观察幼苗叶片解剖结构,光合仪测定幼苗光合参数并以酶联免疫吸附法测叶片内源激素含量,探究外源GA_... 详细信息

以1年生紫斑牡丹幼苗为试验材料,采用不同浓度(0、100、300、500 mg/L)赤霉素(GA_(3))喷施叶片处理,通过透射电镜、扫描电镜、光学显微镜观察幼苗叶片解剖结构,光合仪测定幼苗光合参数并以酶联免疫吸附法测叶片内源激素含量,探究外源GA_(3)对紫斑牡丹幼苗叶片解剖结构、光合特性和内源激素水平的影响。结果表明:(1)低浓度GA_(3)处理的紫斑牡丹叶肉细胞增大,栅栏组织外层细胞中叶绿体数量增加,高浓度GA_(3)处理则与之相反;GA_(3)处理叶片的栅栏组织/海绵组织比值(P/S)、组织结构紧密度(CTR)均下降,而其组织结构疏松度(SR)增加;GA_(3)处理的幼苗叶片的叶肉细胞内各叶绿体大小显著大于对照,随着GA_(3)处理浓度增加,紫斑牡丹叶肉细胞内叶绿体的体积趋于增大,类囊体垛叠凝聚逐渐松散,叶绿体上淀粉颗粒在300 mg/L GA_(3)处理中较明显;叶片气孔长度、宽度、气孔器大小、气孔开度和气孔密度随着GA_(3)浓度升高先升高后下降,同时叶片上表皮角质层厚度随GA_(3)浓度的升高而增加。(2)紫斑牡丹叶片净光合速率(Pn)、气孔导度(Cond)、蒸腾速率(Tr)、水分利用率(WUE)在100和300 mg/L GA_(3)处理下大都显著高于对照,且300 mg/L GA_(3)处理显著高于其余处理,而其在500 mg/L GA_(3)处理下显著低于对照。(3)紫斑牡丹叶片脱落酸(ABA)和吲哚乙酸(IAA)含量均在500 mg/L GA_(3)下显著高于对照,而在其余浓度处理下不同程度低于对照,叶片内源玉米素核苷(ZR)和GA_(3)含量均在300 mg/L GA_(3)处理下显著高于其余处理和对照,而其余处理相比对照均无显著变化;叶片的ZR/ABA、ZR/IAA、ZR/GA_(3)和(IAA+GA_(3)+ZR)/ABA比值都在300 mg/L GA_(3)处理下显著高于其他处理,叶片的IAA/ABA和ABA/GA_(3)比值均在500 mg/L GA_(3)处理下显著高于其他处理。研究发现,适宜浓度外源GA_(3)处理,能显著提高紫斑牡丹幼苗叶片光合速率、水分利用效率及蒸腾速率,调节植物体内源激素的含量及平衡,从而使叶片能合成较多有机物,促进幼苗生长。

关键词：紫斑牡丹赤霉素解剖结构超微结构光合特性内源激素