检索结果-南通市图书馆

Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2024年第7期23卷 2292-2305页

作者： shuang pei Zexu Wu Ziqiao Ji Zheng Liu Zicheng Zhu Feishi Luan Shi Liu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and RuralAffairs/College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030China Institute of Rural Revitalization Science and Technology Heilongjiang Academy of Agricultural SciencesHarbin 150028China

Stigma color is a critical agronomic trait in watermelon that plays an important role in ***,there are few reports on the regulation of stigma color in *** this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow *** segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma ***,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated ***97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon *** expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing *** color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma *** data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma *** study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

关键词： watermelon stigma color gene mapping zinc finger protein CONSTANS-LIKE 4

剪切增稠液增强高发泡率废纸缓冲材料的制备及性能

在线全文

学校读者我要写书评

暂无评论

材料导报 2024年第1期38卷 278-287页

作者：张斌陶文轩裴爽任子铭潘政苟进胜北京林业大学材料科学与技术学院木材科学与工程北京市重点实验室北京100083 中国林业科学院林产化学工业研究所南京210042

随着经济的快速增长和人们环保意识的不断增强,植物纤维类环保型发泡缓冲材料逐渐成为研究热点。为改善植物纤维发泡材料发泡率低、缓冲吸能效果差等问题,本研究设计半密闭型发泡模具,成功制备了具有高发泡率和优异吸能性的废纸缓冲材... 详细信息

随着经济的快速增长和人们环保意识的不断增强,植物纤维类环保型发泡缓冲材料逐渐成为研究热点。为改善植物纤维发泡材料发泡率低、缓冲吸能效果差等问题,本研究设计半密闭型发泡模具,成功制备了具有高发泡率和优异吸能性的废纸缓冲材料并对制备工艺进行了优化。结果表明:使用质量分数为0.5%的氢氧化钠溶液预处理废纸浆,不仅可以有效去除废纸中的杂质,而且能在不降低纤维长度的情况下对废纸纤维进行适当改性,有利于提高废纸缓冲材料的发泡率和整体性能。以正交优化试验研究原料配比对废纸缓冲材料性能的影响,并据此确定了最优配方。通过设计全新半密闭型发泡模具,成功制备高发泡率的废纸缓冲材料,其膨胀率相较传统制备方式提高了3~4倍。本研究首次引入剪切增稠液(STF)增强废纸纤维,考察了STF含量对废纸缓冲材料力学性能、发泡倍率和回弹性能的影响,分析了STF对废纸缓冲材料的增强机理。结果表明:STF能够提高单根废纸纤维的强度并增强纤维之间的连结,从而提升缓冲材料吸能性,且随着STF含量的增加,材料的吸能性逐渐增强,发泡率逐渐降低,当STF含量为10%(质量分数,下同)时,STF增强废纸缓冲材料的综合性能达到最优。

关键词：废纸纤维剪切增稠液缓冲包装材料高发泡率

益气通腑法中药灌肠辅助治疗慢性阻塞性肺疾病合并呼吸衰竭

在线全文

学校读者我要写书评

暂无评论

长春中医药大学学报 2024年第6期40卷 650-654页

作者：裴爽李华裴丽红王景齐齐哈尔市中医医院黑龙江齐齐哈尔161002 黑龙江中医药大学哈尔滨150001

目的探讨益气通腑法中药灌肠辅助治疗慢性阻塞性肺疾病合并呼吸衰竭疗效。方法按照随机数表法将82例慢性阻塞性肺疾病伴呼吸衰竭患者分成对照组与观察组,各41例。对照组在西医常规治疗基础上予无创正压通气等西医治疗,观察组在对照组基... 详细信息

目的探讨益气通腑法中药灌肠辅助治疗慢性阻塞性肺疾病合并呼吸衰竭疗效。方法按照随机数表法将82例慢性阻塞性肺疾病伴呼吸衰竭患者分成对照组与观察组,各41例。对照组在西医常规治疗基础上予无创正压通气等西医治疗,观察组在对照组基础上联合益气通腑法中药灌肠治疗。观察2组治疗前后肺功能指标、血清炎性指标肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP)、白细胞介素8(IL-8),血气分析指标动脉血氧分压(PaO_(2))、血氧饱和度(SaO_(2))、二氧化碳分压(PaCO_(2)),慢阻肺病评估量表(CAT)评分,mMRC评分,慢性阻塞性肺疾病生存状况评估量表(BODE)评分变化,并比较2组治疗总有效率和不良反应总发生率。结果观察组治疗后各项肺功能指标、PaO_(2)、SaO_(2)指标及治疗总有效率均高于对照组(P<0.05),TNF-α、CRP、IL-8、PaCO_(2)、CAT评分、mMRC评分、BODE评分及不良反应总发生率均低于对照组(P<0.05)。结论益气通腑法中药灌肠能够提高慢性阻塞性肺疾病伴呼吸衰竭患者肺通气功能,调节血气指标,抑制体内炎症反应,改善临床预后,且不良反应较少。

关键词：慢性阻塞性肺疾病 II型呼吸衰竭血气分析指标肺功能

滋肾清热利湿化瘀方治疗多囊卵巢综合征的网络药理学作用机制及实验研究

在线全文

学校读者我要写书评

暂无评论

中国药理学通报 2024年第4期40卷 764-775页

作者：金晶李佩双丁志玲彭佳华刘淑娟梁瑞宁江西中医药大学研究生院江西南昌330004 江西中医药大学附属医院江西南昌330006 江西中医药大学中医妇产科学研究所江西南昌330006 江西中医药大学附属生殖医院江西南昌330001 江西中医药大学第二附属医院江西南昌330006

目的采用网络药理学探讨滋肾清热利湿化瘀方(Zishen Qingre Lishi Huayu Prescription,ZQLHP)治疗多囊卵巢综合征(polycystic ovary syndrome,PCOS)的作用机制,并进行实验验证。方法基于网络药理学预测滋肾方的核心成分、靶基因和主要通... 详细信息

目的采用网络药理学探讨滋肾清热利湿化瘀方(Zishen Qingre Lishi Huayu Prescription,ZQLHP)治疗多囊卵巢综合征(polycystic ovary syndrome,PCOS)的作用机制,并进行实验验证。方法基于网络药理学预测滋肾方的核心成分、靶基因和主要通路,对其中PCOS相关成分、靶基因、通路进行筛选;采用来曲唑(letrozole,LET)诱导PCOS小鼠模型,观察滋肾方对PCOS小鼠的作用,并对网络药理学结果进行机制验证。结果网络药理学结果表明,滋肾方456个成分中含有361个相关靶点,PCOS相关靶点1397个,交集靶点125个;获得槲皮素、豆甾醇、β-谷甾醇等主要活性成分与丝裂原活化蛋白激酶3(mitogen-activated protein kinase 3,MAPK3)、蛋白激酶1(AKT serine/threonine kinase 1,AKT1)和信号转导和转录激活因子3(signal transducer and activator of transcription 3,STAT3)等核心靶点;GO分析得到BP 663条目,CC 64条目,MF 119条目,其中富集基因数量较多的条目为negative regulation of apoptotic process,plasma membrane,protein binding;KEGG分析显示潜在作用靶点主要富集在癌症信号通路、PI3K-AKT和TNF等信号通路;PPI网络分析结果显示,MAPK3、AKT1和STAT3可能是滋肾方治疗PCOS的关键靶点。动物实验结果表明,与模型组比较,滋肾方组的小鼠的体重降低(P<0.05),且能有效改善PCOS模型小鼠的组织病理学表现,囊性卵泡数量降低(P<0.05),黄体数量增加(P<0.01)。降低卵巢组织中IL-10水平(P<0.01),升高IL-6、TNF-α水平(P<0.05,P<0.01)。Western blot和免疫组织化学(immunohistochemistry,IHC)结果显示,PCOS小鼠卵巢组织p-AKT蛋白表达量下降(P<0.05,P<0.01),p-p38MAPK和p-STAT3蛋白表达较正常组升高(P<0.05,P<0.01),而经滋肾方治疗后p-AKT蛋白表达上升(P<0.05),p-p38MAPK和p-STAT3蛋白表达明显下降(P<0.05,P<0.01)。结论滋肾方可能通过调控AKT/p38MAPK/STAT3信号改善来曲唑诱导的PCOS小鼠的炎性损伤。

关键词：滋肾清热利湿化瘀方多囊卵巢综合征网络药理学机制 AKT p38MAPK STAT3

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

锌浸渣浮选银精矿还原焙烧-低酸浸出脱锌

有色金属（冶炼部分） 2024年第7期 25-32页

作者：黄玉柱裴双刘宇倩周合理朱晓红胡宇杰湖南工业大学材料与先进制造学院湖南株洲412007

以某湿法炼锌厂产出的锌浸渣浮选银精矿为原料,采用回转炉还原焙烧—低酸浸出工艺进行脱锌研究。结果表明:在碳粉加入量10%、焙烧温度600℃、时间120 min、原料粒度-0.1 mm、回转炉转速5 r/min的条件下,铁酸锌还原焙烧分解效果最优,焙... 详细信息

以某湿法炼锌厂产出的锌浸渣浮选银精矿为原料,采用回转炉还原焙烧—低酸浸出工艺进行脱锌研究。结果表明:在碳粉加入量10%、焙烧温度600℃、时间120 min、原料粒度-0.1 mm、回转炉转速5 r/min的条件下,铁酸锌还原焙烧分解效果最优,焙烧产物中可溶锌率达到85.83%。在硫酸终点pH为2、液固比5 mL/g、浸出温度70℃、浸出时间90 min优化条件下,对焙烧产物进行选择性浸出,锌和铁的浸出率分别为84.23%和34.71%。浸出渣中未溶解铁锌氧化物主要为还原焙烧过程中团聚成块的大颗粒及被硫酸铅熔化包裹形成的颗粒。

关键词：锌浸渣浮选银精矿铁酸锌还原焙烧低酸浸出脱锌工艺

Successful remimazolam sedation-epidural block in an older patient with severe chronic obstructive pulmonary disease:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第14期11卷 3330-3339页

作者： Jia-Jia Yu Huan-shuang pei Yu Meng Department of Anesthesiology The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Chronic obstructive pulmonary disease(COPD)is associated with high morbidity and mortality rates *** patients have a degenerative cardiopulmonary function,weak compensatory capacity,and poor surgical ***,the mode of anesthesia must be *** is a new ultrashort-acting benzodiazepine with a rapid onset of action,rapid metabolism,and mild effects on pulmonary *** sedation combined with an epidural block has not been reported in hypertensive older adults with severe COPD and inguinal mass *** SUMMARY We report the case of a 73-year-old man with hypertension and severe COPD,who underwent resection of an enlarged inguinal mass that he had noticed more than 7 mo before *** patient presented with a“right inguinal mass”and was recommended to undergo an enlarged inguinal mass *** was relatively challenging,due to the large mass(13 cm×8 cm×7 cm),hard texture,and poor *** the advanced age of the patient,gradeⅢhypertension,and severe COPD,we administered remimazolam combined with an epidural block for anesthesia to ensure perioperative safety and careful *** anesthetic effect was precise;the procedure was performed smoothly without any complications,and the patient was successfully ***,anesthetic management in such cases has not yet been reported by previous *** Remimazolam sedation combined with an epidural block is safe and effective in older patients with hypertension and severe COPD.

关键词： Remimazolam Older adult Chronic obstructive pulmonary disease Epidural block Case report

Hyperlactemia associated with secondary hepatocellular carcinoma resection in relation to circulation stability and quality of recovery:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第8期11卷 1869-1877页

作者： Yu Meng Huan-shuang pei Jia-Jia Yu Department of Anesthesiology The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Intraoperative hyperlactatemia often affects circulatory stability,vital organ function,and postoperative recovery,poses a serious prognostic risk,and requires considerable attention from ***,we describe a case of hyperlactatemia during the postoperative resection of liver metastases after chemotherapy for sigmoid colon *** did not affect the patient's circulatory stability or quality of awakening,which is rarely reported in clinical *** present our management experience with the aim of providing a reference for future studies and clinical *** SUMMARY A 70-year-old female patient was diagnosed with postoperative liver metastasis following chemotherapy for sigmoid colon *** right hemicolectomy and cholecystectomy under general anesthesia were *** disorders,primarily hyperlactatemia,often occur *** treatment,other indices quickly returned to normal,lactate levels decreased slowly,and hyperlactatemia persisted during the awakening ***,this did not affect the patient's circulatory stability or awakening *** condition has rarely been clinically ***,we report our management experience in order to guide clinical practice in this *** did not affect circulatory stability or the quality of *** considered that active intraoperative rehydration avoided serious harm to the organism caused by hyperlactatemia due to insufficient tissue perfusion,while hyperlactatemia caused by decreased lactate clearance due to impaired liver function associated with surgical resection had a mild effect on the function of important *** Active intraoperative rehydration avoided serious harm to the organism caused by *** body temperature protection could improve lactate circulation.

关键词： Circulation Hyperlactatemia Laparoscopy Quality of awakening Secondary hepatocellular carcinoma Case report

一种航空涡轮发动机传动零件轴转子的加工方法研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

机械工程师 2024年第6期 31-33,37页

作者：宋媛张立明齐琦王贵春裴爽姚远黑龙江省机械科学研究院哈尔滨150040

某航空涡轮发动机传动零件轴转子在加工过程中让刀现象严重,刀具磨损过快,叶片加工尺寸不稳定,叶片表面容易形成振纹。为了解决这一问题,介绍了一种加工工艺,并给出了合适的刀具选择。

关键词：细长杆结构高温合金振纹

Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosvnthesis

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2015年第6期8卷 911-921页

作者： Gao-Jun Liu Guang-Hui Xiao Ning-Jing Liu Dan Liu pei-shuang Chen Yong-Mei Qin Yu-Xian Zhu The State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China

The membrane lipids from fast-elongating wild-type cotton （Gossypium hirsutum） fibers at 10 days post- anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. Fiber cells contained significantly higher amounts of phosphatidylinositol （PI） than both ovule samples with PI 34：3 being the most predominant spe- cies. The genes encoding fatty acid desaturases （415GhFAD）, PI synthase （PIS） and PI kinase （PIK） were expressed in a fiber-preferential manner. Further analysis of phosphatidylinositol monophosphate （PIP） indicated that elongating fibers contained four- to five-fold higher amounts of PIP 34：3 than the ovules. Exog- enously applied linolenic acid （C18：3）, soybean L-α-PI, and PIPs containing PIP 34：3 promoted significant fiber growth, whereas a liver PI lacking the C18：3 moiety, linoleic acid, and PIP 36：2 were completely ineffec- tive. The growth inhibitory effects of carbenoxolone, 5-hydroxytryptamine, and wortmannin were reverted by C18：3, PI, or PIP, respectively, suggesting that PIP signaling is essential for fiber cell growth. Furthermore, cotton plants expressing virus-induced gene-silencing constructs that specifically suppressed Gh15FAD, GhPIS, or GhPIK expression, resulted in significantly short-fibered phenotypes. Our data provide the basis for in-depth studies on the roles of PI and PIP in mediating cotton fiber growth.

关键词： Gossypium hirsutum fiber elongation mass spectrometry glycerophospholipid profiling phosphati-dylinositol phosphatidylinositol monophosphate