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Transcriptome and genome analysis to identify C2H2 genes participating in low-temperature conditioning-alleviated postharvest chilling injury of peach fruit

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Food Quality and Safety 2022年第4期6卷 500-509页

作者： Yaqin Zheng Zhenning liu Hui Wang Wenna Zhang shuaijie li Meng Xu College of Agriculture and Forestry Sciences Linyi UniversityLinyiChina Shandong(linyi)Institute of Modern Agriculture Zhejiang UniversityLinyiChina

Objectives:This work intended to identify candidate C2H2 genes participating in low-temperature conditioning(LTC)-alleviated postharvest chilling injury of peach *** and Methods:For LTC treatment,fruit were pre-stored at 10℃for 5 d and then transferred to 0℃*** firmness was measured by a hardness tester.H_(2)O_(2)content was determined by luminosity measurement model using a multifunctional enzyme *** of C2H2 family members was performed by HMMSCAN according to peach *** cis-acting element of gene promoters was analyzed using the Plant CARE *** gene coexpression network analysis(WGCNA)was performed by the WGCNA package in the BMK Cloud ***:LTC treatment decreased flesh browning rate and H_(2)O_(2)production of‘Beijing No.9’*** factor identification of differentially expressed genes in 0℃and the LTC treatment indicated that peach C2H2 participated in the regulation of chilling injury.A total of 47 C2H2 genes were identified based on peach ***-time quantitative polymerase chain reaction(qRT-PCR),phylogenetic analysis and promoter cis-acting element analysis revealed that ZFP21 was involved in the regulation of LTC-alleviated chilling injury in *** and dual luciferase assay suggested that ZFP21 participated in LTC-alleviated chilling injury by downregulating the expression of reactive oxygen species-related genes ***:Our investigation,based on genome and RNA-seq,revealed that ZFP21 was involved in LTC treatment-alleviated chilling injury of peach *** work is useful for the identification of peach cold tolerance-related genes and the study of C2H2 family in peach.

关键词： Peach fruit low-temperature conditioning chilling injury C2H2 cold storage

Potential of octant degeneracy resolution in JUNO

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Chinese Physics C 2019年第3期43卷 17-21页

作者： M.V.Smirnov Zhoujun Hu shuaijie li Jiajie ling Department of physics Sun Yat-Sen UniversityGuangzhou 510275China

This work extends the idea of using a cyclotron-based antineutrino source for purposes of neutrino *** baseline experiments suffer from degeneracies and correlations between Θ_(23), δ_(CP) and the mass ***, the combination of a superconducting cyclotron and a big liquid scintillator detector like JUNO in a medium baseline experiment, which does not depend on the mass hierarchy, may allow to determine whether the position of the mixing angle Θ_(23) is in the lower octant or the upper octant. Such an experiment would improve the precision of the Θ_(23) measurement to a degree which depends on the CP-phase.

关键词： neutrino oscillations octant mixing angle JUNO

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自催化邻苯二甲腈树脂的研究进展

中国胶粘剂 2024年第7期33卷 13-21页

作者：李帅杰刘彩召孙明明张绪刚李坚辉张斌黑龙江省科学院石油化学研究院黑龙江哈尔滨150040

邻苯二甲腈树脂是一类具有优异综合性能的热固性树脂,在航空航天、船舶制造、电子封装等使用环境苛刻的领域中有着广泛的应用需求。然而邻苯二甲腈树脂本身固化非常缓慢,设计合成自催化邻苯二甲腈树脂分子结构是一种有效的解决方式。本... 详细信息

邻苯二甲腈树脂是一类具有优异综合性能的热固性树脂,在航空航天、船舶制造、电子封装等使用环境苛刻的领域中有着广泛的应用需求。然而邻苯二甲腈树脂本身固化非常缓慢,设计合成自催化邻苯二甲腈树脂分子结构是一种有效的解决方式。本文将自催化邻苯二甲腈归纳为酚羟基、氨基、仲胺、席夫碱、咪唑、苯并噁嗪、烷烃、马来酰亚胺和自由基等九大类,分别综述了不同类型树脂近几年的研究进展情况,并对自催化邻苯二甲腈树脂今后的发展趋势进行了展望。

关键词：自催化邻苯二甲腈树脂分子结构固化机理

Research on Leading Edge Erosion and Aerodynamic Characteristics of Wind Turbine Blade Airfoil

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Fluid Dynamics & Materials Processing 2024年第9期20卷 2045-2058页

作者： Xin Guan Yuqi Xie shuaijie Wang Mingyang li Shiwei Wu School of New Energy Shenyang Institute of EngineeringShenyang110136China

The effects of the erosion present on the leading edge of a wind turbine airfoil(DU 96-W-180)on its aerodynamic performances have been investigated numerically in the framework of a SST k–ωturbulence model based on the Reynolds Averaged Navier-Stokes equations(RANS).The results indicate that when sand-induced holes and small pits are involved as leading edge wear features,they have a minimal influence on the lift and drag coefficients of the ***,if delamination occurs in the same airfoil region,it significantly impacts the lift and resistance characteristics of the ***,as the angle of attack grows,there is a significant decrease in the lift coefficient accompanied by a sharp increase in the drag *** wear intensifies,these effects gradually ***,the leading edge wear can exacerbate flow separation near the trailing edge suction surface of the airfoil and cause forward displacement of the separation point.

关键词： Wind energy wind turbine erosion airfoil leading edge erosion characteristics aerodynamic performance numerical simulation

Measurement of solar pp neutrino flux using electron recoil data from PandaX-4T commissioning run

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Chinese Physics C 2024年第9期48卷 1-7,300页

作者： Xiaoying Lu Abdusalam Abdukerim Zihao Bo Wei Chen Xun Chen Yunhua Chen Chen Cheng Zhaokan Cheng Xiangyi Cui Yingjie Fan Deqing Fang lisheng Geng Karl Giboni Xuyuan Guo Chencheng Han Ke Han Changda He Jinrong He Di Huang Junting Huang Zhou Huang Ruquan Hou Yu Hou Xiangdong Ji Yonglin Ju Chenxiang li Jiafu li Mingchuan li shuaijie li Tao li Qing lin Jianglai liu Congcong Lu lingyin Luo Yunyang Luo Wenbo Ma Yugang Ma Yajun Mao Yue Meng Xuyang Ning Binyu Pang Ningchun Qi Zhicheng Qian Xiangxiang Ren Nasir Shaheed Xiaofeng Shang Xiyuan Shao Guofang Shen Manbin Shen lin Si Wenliang Sun Yi Tao Anqing Wang Meng Wang Qiuhong Wang Shaobo Wang Siguang Wang Wei Wang Xiuli Wang Xu Wang Zhou Wang Yuehuan Wei Mengmeng Wu Weihao Wu Yuan Wu Mengjiao Xiao Xiang Xiao Kaizhi Xiong Binbin Yan Xiyu Yan Yong Yang Chunxu Yu Ying Yuan Zhe Yuan Youhui Yun Xinning Zeng Minzhen Zhang Peng Zhang Shibo Zhang Shu Zhang Tao Zhang Wei Zhang Yang Zhang Yingxin Zhang Yuanyuan Zhang li Zhao Jifang Zhou Ning Zhou Xiaopeng Zhou Yubo Zhou Zhizhen Zhou Research Center for Particle Science and Technology Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237China Key Laboratory of Particle Physics and Particle Irradiation of Ministry of Education Shandong UniversityQingdao 266237China School of Physics and Astronomy Shanghai Jiao Tong UniversityKey Laboratory for Particle Astrophysics and Cosmology(MoE)Shanghai Key Laboratory for Particle Physics and CosmologyShanghai 200240China New Cornerstone Science Laboratory Tsung-Dao Lee InstituteShanghai Jiao Tong UniversityShanghai 200240China Shanghai Jiao Tong University Sichuan Research Institute Chengdu 610213China Jinping Deep Underground Frontier Science and Dark Matter Key Laboratory of Sichuan Province Liangshan 615000China Yalong River Hydropower Development Company Ltd.288 Shuanglin RoadChengdu 610051China School of Physics Sun Yat-Sen UniversityGuangzhou 510275China Sino-French Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Department of Physics Yantai UniversityYantai 264005China Key Laboratory of Nuclear Physics and Ion-beam Application(MOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China School of Physics Beihang UniversityBeijing 102206China Beijing Key Laboratory of Advanced Nuclear Materials and Physics Beihang UniversityBeijing 102206China Peng Huanwu Collaborative Center for Research and Education Beihang UniversityBeijing 100191China Southern Center for Nuclear-Science Theory(SCNT) Institute of Modern PhysicsChinese Academy of SciencesHuizhou 516000China School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China Department of Physics University of MarylandCollege ParkMaryland 20742USA State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China School of Physics Peking UniversityBeijing 100871Ch

The proton-proton(pp)fusion chain dominates the neutrino production in the *** uncertainty of the predicted pp neutrino flux is at the sub-percent level,whereas that of the best measurement is O(10%).In this study,for the first time,we measure solar pp neutrinos in the electron recoil energy range from 24 to 144 keV using the PandaX-4T commissioning data with 0.63 tonne×year *** pp neutrino flux is determined as(8.0±3.9(stat)±10.0(syst))×1010 s^(-1)cm^(-2),which is consistent with the Standard Solar Model and existing measurements,corresponding to an upper flux limit of 23.3×10^(10)s^(-1)cm^(-2)at 90%C.L..

关键词： solar pp neutrino flux PandaX-4T electron recoil spectrum

穴居植物湖南报春苣苔大小孢子发生及雌雄配子体发育研究

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中国野生植物资源 2024年第4期43卷 39-44页

作者：陈灵灵李帅杰邹雅吴尧晶许海霞蔡秀珍湖南师范大学生命科学学院湖南长沙410081 中国科学院武汉植物园湖北武汉430074 长沙市北雅中学湖南长沙410008 怀化师范高等专科学校湖南怀化418000

目的:湖南报春苣苔(Primulina hunanensis)为我国特有的极具观赏价值的岩溶洞穴野生植物,具耐荫、耐贫瘠等特性,但其自然分布范围狭窄、野生种群规模小。胚胎发育是植物生活史中的关键环节,研究湖南报春苣苔的胚胎发育特征,为苦苣苔科(G... 详细信息

目的:湖南报春苣苔(Primulina hunanensis)为我国特有的极具观赏价值的岩溶洞穴野生植物,具耐荫、耐贫瘠等特性,但其自然分布范围狭窄、野生种群规模小。胚胎发育是植物生活史中的关键环节,研究湖南报春苣苔的胚胎发育特征,为苦苣苔科(Gesneriaceae)植物的栽培育种提供新思路。方法:以湖南报春苣苔为材料,运用经典石蜡切片技术,对大、小孢子发生及雌、雄配子体发育过程进行解剖学观察。结果:湖南报春苣苔花药4室,花药壁由外到内依次为表皮、药室内壁、中层和腺质型绒毡层;小孢子母细胞减数分裂时的胞质分裂为同时型,四分体偶为交叉(十字)形,多为四面体形,花粉为二细胞型;倒生胚珠,单珠被,薄珠心,胚囊发育方式为蓼型。湖南报春苣苔花粉粒发育不同步,存在花粉败育、雌雄配子体异熟现象。结论:研究为报春苣苔属(Primulina)植物积累了基础的胚胎学资料,对于揭示苦苣苔科植物生殖机制及改良植物繁殖育种方式具有重要意义。

关键词：湖南报春苣苔大孢子发生小孢子发生雌配子体雄配子体

基于CESO的SIDO Buck-Boost变换器自抗扰控制策略

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湖南大学学报（自然科学版） 2023年第10期50卷 41-50页

作者：皇金锋李帅杰陕西理工大学电气工程学院陕西汉中723001

针对单电感双输出Buck-Boost变换器两输出支路va和vb间存在交叉影响,暂态性能差等问题,提出一种基于级联扩张状态观测器(CESO)的自抗扰控制(ADRC)策略.首先,根据ADRC理论将主路和支路分别拟合为相互独立的系统,减少支路间的交叉影响;其... 详细信息

针对单电感双输出Buck-Boost变换器两输出支路va和vb间存在交叉影响,暂态性能差等问题,提出一种基于级联扩张状态观测器(CESO)的自抗扰控制(ADRC)策略.首先,根据ADRC理论将主路和支路分别拟合为相互独立的系统,减少支路间的交叉影响;其次,设计CESO对系统总扰动进行估计,消除因传统扩张状态观测器不完全估计而造成的观测残差,并利用CESO对电路传感器高频噪声的抑制作用,进一步提高观测值的估计精度;然后,将观测值作用于ADRC的状态误差反馈控制率,以提高系统的暂态性能;最后,利用Lyapunov理论证明了控制系统的闭环稳定性.仿真结果表明:本文所提控制策略有效减小了两输出支路va和vb间的交叉影响,同时提高了系统的暂态性能.

关键词：单电感双输出Buck-Boost变换器交叉影响级联扩张状态观测器自抗扰控制

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Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects

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生物活性材料(英文) 2023年第5期23卷 101-117页

作者： shuaijie li liyan Zhang Chunyu liu Jua Kim Kun Su Tingli Chen limin Zhao Xiaomei Lu Hao Zhang Yinglin Cui Xu Cui Feng Yuan Haobo Pan Department of Orthopedics Tongji HospitalSchool of MedicineTongji UniversityShanghai200065China Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen518055China Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education and Tianjin College of BiotechnologyTianjin University of Science and Technology300457China Shenzhen Longhua District Central Hospital/the Affiliated Central Hospital of Shenzhen Longhua District Guangdong Medical UniversityShenzhen518110China Department of Orthopedics Shanxi Bethune HospitalTaiyuan030032China Shenzhen Key Laboratory of Marine Biomaterials Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen518055China

Injectable bone biomaterials like bone cement should be designed and fabricated with certain biological criteria,which include:1)recruitment and polarization of the macrophages from M1(pro-inflammatory)to M2(anti-inflammatory)phenotype,2)enhance vascularization,and 3)activate osteogenic differentiation of bone marrow-derived stem cells to promote bone *** far,no injectable biomaterials could spontaneously regulate the entire bone healing process that involves inflammation,angiogenesis,and ***,in this study,we designed bone cement comprised of strontium and copper-incorporated borosilicate glass(Sr/Cu-BSG)in the liquid phase of chitosan to modulate bone *** vitro studies showed that the controlled release of Sr and Cu ions up-regulated anti-inflammatory genes(IL-1Ra and TGF-β1)while down-regulating pro-inflammatory genes(IL-1βand IL-6)in macrophages at 3 *** and Cu ions also increased the expressions of angiogenic genes(VEGF and bFGF)in HUVECs at 5 days and osteogenic genes(Runx-2,OCN,and OPN)in hBMSCs at 7,14,and 21 days.5Sr3Cu-BSG bone cement exhibited the best anti-inflammatory,angiogenic,and osteogenic properties among the bone cement groups with different Sr and Cu ***-term and long-term implantation of Sr/Cu-BSGs in femoral condylar bone defects of rats and rabbits confirmed the in vitro results,where the degradation rate of Sr/Cu-BSG matched the bone healing *** to in vitro,the 5Sr3Cu-BSG group also showed the highest bone formation in *** physical and chemical properties,along with its bone repairing ability,make the Sr/Cu-BSG bone cement a good candidate biomaterial for treating bone defects.

关键词： Borosilicate glass bone cement Immunomodulation Angiogenesis Osteogenesis

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湖南报春苣苔的花部特征及其繁育系统研究

园艺学报 2020年第3期47卷 492-502页

作者：李帅杰蔡秀珍湖南师范大学生命科学学院长沙410081

为揭示湖南报春苣苔(Primulina hunanensis)花部特征和繁育系统对有性繁殖过程的影响,合理开发利用其野生种质资源,对其开花物候、花部特征以及访花昆虫类型进行了野外定点观测,测量和分析了花粉活力及柱头可授性、花粉胚珠比值、杂交指... 详细信息

为揭示湖南报春苣苔(Primulina hunanensis)花部特征和繁育系统对有性繁殖过程的影响,合理开发利用其野生种质资源,对其开花物候、花部特征以及访花昆虫类型进行了野外定点观测,测量和分析了花粉活力及柱头可授性、花粉胚珠比值、杂交指数,并结合人工辅助授粉试验对其繁育系统进行了检测。结果表明,(1)湖南报春苣苔花期在5-7月,长约40 d,单花周期3~8 d;雌雄异熟,开放当天花粉活力最强(>90%),柱头无活性;雌雄异位,开放后雄蕊几乎不再生长,而雌蕊继续伸长。(2)花粉胚珠比值为518.36,杂交指数为5,繁育系统以异交为主,部分自交亲和,需要传粉者;去雄不授粉套袋结实率为0,不存在无融合生殖。(3)开花期间存在多种传粉者,熊蜂与其花部特征相适应,为最有效传粉者。(4)自然状态下结实率较高;存在无性繁殖,作为生殖补偿机制维持种群的数量。

关键词：湖南报春苣苔传粉生物学繁育系统花部特征熊蜂