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Two Divergent Members of 4-Coumarate： Coenzyme A Ligase from Salvia miltiorrhiza Bunge： cDNA Cloning and Functional Study

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Journal of Integrative Plant Biology 2006年第11期48卷 1355-1364页

作者： shu-juan zhao Zhi-Bi Hu DI Liu Frederick C. C. Leungt Institute of Traditional Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Shanghai 201203 China Department of Zoology University of Hong Kong Hong Kong SAR China

4-Coumarate ： coenzyme A Ilgase （4CL） Is one of the key enzymes In phenylpropanoid metabolism leading to series of phenollcs, Including water-soluble phenolic acids, which are important compounds determining the medicinal quality of Danshen （Salvia miltiorrhiza Bunge）, a traditional Chinese medicinal herb. To Investigate the function of 4CL in the biosynthesis of water-soluble phenolic acid in Danshen, we have cloned two cDNAs （Sm4CL1 and Sm4CL2） encoding divergent 4CL members by applying nested reverse transcrlptlon-polymerase chain reaction （RT-PCR） with degenerate primers followed by 5′/3′rapid amplification of cDNA ends （RACE）（Note, these sequence data have been submitted to the GenBank database under accession numbers AY237163 and AY237164）. Either of the coding regions was inserted into a pRSET vector and a kinetic assay was performed with purified recombinant proteins. The substrate utilization profile of Sm4CL1 was distinct from that of Sm4CL2. The Km values of Sm4CL1 and Sm4CL2 to 4-coumarlc acid were （72.20±4.10） and （6.50±1.45） μmol/L, respectively. These results, In conjunction with Northern blotting and other information, imply that Sm4CL2 may play an Important role in the biosynthesis of watersoluble phenolic compounds, whereas Sm4CL1 may play a minor role in the pathway. Southern blotting analysis suggested that both Sm4CL1 and Sm4CL2 genes are present as a single copy and are located at different sites In the genome.

关键词： cDNA cloning 4-coumarate : coenzyme A ligase (4CL) Danshen (Salvia miltiorrhiza) enzymatic kinetic assays water-soluble phenolic acids.

L‑Tyrosine Metabolic Pathway in Microorganisms and Its Application in the Biosynthesis of Plant‑Derived Natural Products

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World Journal of Traditional Chinese Medicine 2022年第3期8卷 386-394页

作者： Yi‑Wen Zhang Feng Nie Xiao‑Yu Zheng shu‑juan zhao The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicine The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese MedicinesInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghaiChina Experiment Center for Teaching and Learning Shanghai University of Traditional Chinese MedicineShanghaiChina

L-tyrosine,an aromatic amino acid,is an important upstream precursor for the synthesis of a series of valuable natural products such as flavonoids and phenolic *** recent years,regulation of the L‑tyrosine metabolic pathway has been devoted to enhancing the production of L-tyrosine and the derived bioactive compounds in microorganisms,usually by increasing the supply of precursors,blocking competitive routes,and modulating the transport ***,we reviewed the strategies to promote L-tyrosine production in microbial hosts and the common strategies to produce bioactive compounds in engineered Escherichia coli and Saccharomyces cerevisiae to better understand and utilize the L-tyrosine metabolic pathway for microbial overproduction of diverse valuable aromatic compounds in the future.

关键词： Biosynthesis Escherichia coli L-tyrosine Saccharomyces cerevisiae

Geochemistry and detrital zircon records of the Ruyang-Luoyu groups,southern North China Craton:Provenance,crustal evolution and Paleo-Mesoproterozoic tectonic implications

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Geoscience Frontiers 2020年第2期11卷 679-696页

作者： Xi-Yao Li Sanzhong Li Tong-Shan Wang Yunpeng Dong Xiao-Guang Liu shu-juan zhao Kun Wang Jiao-Peng Sun Li-Ming Dai Yan-Hui Suo Key Lab of Submarine Geosciences and Prospecting Techniques MOEand Institute for Advanced Ocean StudyCollege of Marine GeosciencesOcean University of ChinaQingdao 266100China State Key Laboratory of Continental Dynamics Northwest UniversityXi’an 710069China Laboratory for Marine Mineral Resources Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Research Institute of Petroleum Exploration and Development PetroChinaBeijing 100083China

Paleo-to Mesoproterozoic sedimentary rocks in the southern margin of the North China Craton(NCC)are represented by the Ruyang and Luoyu *** studied the sedimentary rocks from the Yunmengshan and Beidajian formations of the Ruyang Group and the Cuizhuang and Sanjiaotang formations of the Luoyu *** zircon grains from these formations have U–Pb age populations of 3.64–3.31 Ga,2.96–2.86 Ga,2.72–2.59 Ga,2.56–2.47 Ga,2.45–2.0 Ga,1.99–1.85 Ga and 1.84–1.65 *** geochemical features of the sedimentary rocks suggest that some of the sediments were sourced from intermediate to felsic magmatic *** age groups of the detrital zircon are roughly consistent with the tectono-thermal events in the southern margin of the *** Hf isotopic compositions of detrital zircon from the sedimentary rocks in Ruyang and Luoyu groups suggest that significant crustal growth and reworking of the NCC took place during the Neoarchean and early-to mid-Paleoproterozoic,while crustal reworking at the Paleoarchean and late-Paleoproterozoic,and crustal growth at the *** suggest the depositional times of the Ruyang Group and Luoyu Group are constrained to no older than 1.75–1.7 Ga and 1.7–1.65 Ga,*** of late-Paleoproterozoic basins related to the strike slip and extrusion tectonics that cross-cut the NCC during the late Paleoproterozoic(<1.75 Ga),and the late Paleoproterozoic sedimentation once isochronous developed in the southern margin of the NCC through the Taihang region of the interior NCC and linked the Yanshan–Liaoxi regions of the northern NCC.

关键词： Detrital zircon North China craton Proterozoic Tectonics U–Pb ages Hf isotope

Is it necessary to stent renal artery stenosis patients before cardiopulmonary bypass procedures？

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Chinese Medical Journal 2011年第10期124卷 1453-1457页

作者： ZHENG Bin YAN Hong-bing LIU Rui-fang CHENG shu-juan WANG Jian zhao Han-jun SONG Li Department of Cardiology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

Background Acute kidney injury （AKI） is associated with poor prognosis after cardiopulmonary bypass. The aim of this retrospective study was to investigate whether stent implantation before cardiopulmonary bypass has beneficial effect on development of AKI in renal artery stenosis （RAS） *** In this retrospective study, patients with abnormal baseline serum creatinine （SCr, 〉106 μmol/L） were not included. Included patients （n=69） were divided into two groups. Group 1 included 31 RAS patients receiving no stent implantation before cardiopulmonary bypass. Group 2 included 38 RAS patients having received stent implantation just before cardiopulmonary bypass. To assess AKI after cardiopulmonary bypass, serum urea nitrogen, SCr and creatinine clearance were recorded at baseline, at the end of operation, during the first and second postoperative 24 *** Baseline characteristics were similar between groups. Serum urea nitrogen, SCr, creatinine clearance before and after cardiopulmonary bypass were also similar class groups. Incidence of AKI in group 1 was not significantly different from group 2. In group 1, AKI defined by RIFLE between occurred in 7 （22.6%） patients： 5 （16.1%） with RIFLE-R,2 （6.5%） with RIFLE-I, and no patients with RIFLE-F. In group 2, 10 patients （26.3%） had an episode of AKI during hospitalization： 6 （15.8%） had RIFLE-R, 4 （10.5%） had RIFLE-I, and no patients had *** There are no data suggesting that it is necessary to stent RAS patients with normal SCr before cardiopulmonary bypass. However, it cannot be concluded that RAS is not associated with AKI after cardiopulmonary bypass

关键词： renal insufficiency cardiopulmonary bypass peripheral vascular disease renal artery

Distribution and diversity of endophytic fungi in Gentiana rigescens and cytotoxic activities

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Chinese Herbal Medicines 2020年第3期12卷 297-302页

作者： Li-li Xu Xiao-feng Ling shu-juan zhao Ru-feng Wang Zheng-tao Wang Institute of Science Technology and HumanitiesShanghai University of Traditional Chinese MedicineShanghai 201203China MOE Key Laboratory for Standardization of Chinese Medicines SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese MedicinesInstitute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China

Objective:In the present study,Gentiana rigescens was screened for fungi communities to clarify their diversity and community assemblage in ***,the identification and activity assays of the strains were also ***:By culture-dependent(endophytic fungi isolations from plant sections)and culture-independent(metagenomic library and cloning from plant sections)techniques,fungi communities were *** metagenomic library was generated using direct DNA isolation of whole plants,plant radixes,plant stems,plant leaves,plant flowers and soils around the ***,endophytes were isolated from all parts *** *** fermentation of the fungi isolations,all the isolates were evaluated for their cytotoxicity against four kinds of human cancer cell lines(HCT116,BEL7404,A549,MDA-MB-231).Results:Eventually,200 strains were isolated and 103 strains were further identified through the internal transcribed spacer(ITS,ITS1 and ITS2 regions)sequence by using the universal primers ITS5 and ITS4.A total of 59,106 fungal sequences corresponding to 374 putative operational taxonomic units(OTU)were identified by 454 *** 454 pyrosequencing,the main fungal genera were Sebacina,Botrytis,Mycosphaerella,Boletus and Gibberella,and the major fungal genera which were directly isolated were Aspergillus,Fusarium,Penicillium and *** assays showed strains 5-26(Aspergillus sp.)and 6-2(Fusarium avenaceum)had the outstanding cytotoxicity to all the tested cell lines with IC5o values<5μg/***:This study revealed the abundance of endogenetic fungal resources and a variety of genetic information in *** by high-throughput 454 sequencing technology and fungi isolation *** assays indicated that endophytes were a promising natural source of potential anticancer agents.

关键词： cytotoxic activities endophytic fungi Gentiana rigescens Franch. ITS metagenomic library

A Mn-porphyrinic metal–organic framework immobilizing glucose oxidase for combined photodynamic/chemodynamic/starvation therapy

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Tungsten 2025年第1期7卷 183-194页

作者： Xin-Ran Sun Hao-Ming Yuan Guo-Da Zhang Chao Wang shu-juan Sun Peng-Fei Shi Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers School of Chemistry and Chemical Engineering Linyi University

The combination of photodynamic therapy（PDT） and chemodynamic therapy（CDT） in tumor treatment has attracted considerable ***,tumor hypoxia and glutathione（GSH） overproduction in the tumor tissue restricted the progress of their ***,a Mn-porphyrinic metal-organic framework（Mn-TCPP） was constructed by the one-pot method and further used for immobilizing glucose oxidase（GOx） to obtain GOx@*** would rapidly exhaust endogenous glucose into hydrogen peroxide（H2O2） and gluconic acid,thus shutting off the energy supply of tumor cells for starvation ***-TCPP catalyzed H2O2to produce oxygen,regulating the hypoxic tumor microenvironment and in turn improving1O2generation under laser ***,Mn-TCPP can reduce reactive oxygen species consumption owing to the redox reaction between Mn3+and GSH,thus greatly enhancing ***,benefiting from GOx-mediated starvation therapy,Mn2+produced by Mn3+reduction can react with sufficient intracellular H2O2to generate ·OH with high cytotoxicity through a Fenton-like *** treatment by GOx@Mn-TCPP under laser irradiation in vitro,the cell viability of 4T1 and A549 tumor cells reached to 20%,reflecting GOx@Mn-TCPP could give full play to the advantages of PDT/CDT/starvation *** results in vivo demonstrated that GOx@Mn-TCPP mediated synergistic cascade therapy could significantly inhibit tumor growth and improve the therapeutic effect.

关键词： Chemodynamic therapy Immobilizing enzyme Metal–organic frameworks Photodynamic therapy Starvation therapy

Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating YAP1 and autophagy

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Neural Regeneration Research 2025年第3期20卷 887-899页

作者： Jianan Wang Yuanyuan Ran Zihan Li Tianyuan zhao Fangfang Zhang juan Wang Zongjian Liu Xuechai Chen Beijing International Science and Technology Cooperation Base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk B homolog 5(ALKBH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(YAP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of YAP1 promoted autophagy,indicating that the mutual regulation between YAP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through YAP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKBH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol YAP1 YTHDF2

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PEG改性氧化钨薄膜的电致变色特性

无机材料学报 2017年第2期32卷 185-190页

作者：路淑娟王唱赵博文汪浩刘晶冰严辉北京工业大学材料学院北京100124

为改善WO_3薄膜的电致变色性能,采用溶胶–凝胶法制备了聚乙二醇(PEG)改性的WO_3电致变色薄膜,并对其着色态与漂白态的光学特性以及循环伏安特性进行了研究。研究表明:PEG改性的WO_3薄膜具有良好的电致变色性能,循环5000次伏安曲线无明... 详细信息

为改善WO_3薄膜的电致变色性能,采用溶胶–凝胶法制备了聚乙二醇(PEG)改性的WO_3电致变色薄膜,并对其着色态与漂白态的光学特性以及循环伏安特性进行了研究。研究表明:PEG改性的WO_3薄膜具有良好的电致变色性能,循环5000次伏安曲线无明显衰减,对可见光的最大透过率调制幅度可达71%。PEG的加入改变了WO_3薄膜的微结构,形成了平均直径为9 nm的介孔,提高了离子在其中的扩散速率,因此改善了WO_3薄膜的电致变色性能。由于循环稳定性对于电致变色材料的实际应用至关重要,因此这种低成本的湿化学法有望用于制备高性能的WO_3基电致变色器件。

关键词： WO3薄膜电致变色溶胶–凝胶聚乙二醇改性

Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing Zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige Song Chao Xu Kai zhao Guoqiang Liu Youming Lu Xinyan Li Kai shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury