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Differences of core genes in liver fibrosis and hepatocellular carcinoma:Evidence from integrated bioinformatics and immunohistochemical analysis

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1265-1280页

作者： Yue li shou-li yuan Jing-Ya Yin Kun Yang Xin-Gang Zhou Wen Xie Qi Wang Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Key Laboratory of Emerging Infectious Diseases Beijing 100015China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyBeijing 100101China Center of liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

BACKGROUND liver fibrosis and hepatocellular carcinoma(HCC)are common adverse consequences of chronic liver *** interaction of various risk factors may cause them to *** of specific biomarkers is of great significance for understanding the occurrence,development mechanisms,and determining the novel tools for diagnosis and treatment of both liver fibrosis and *** To identify liver fibrosis-related core genes,we analyzed the differential expression pattern of core genes in liver fibrosis and *** Gene expression profiles of three datasets,GSE14323,GSE36411,and GSE89377,obtained from the Gene Expression Omnibus(GEO)database,were analyzed,and differentially expressed genes(DEGs)between patients with liver cirrhosis and healthy controls were identified by screening via R software packages and online tool for Venn *** WebGestalt online tool was used to identify DEGs enriched in biological processes,molecular functions,cellular components,and Kyoto Encyclopedia of Genes and Genomes *** protein–protein interactions of DEGs were visualized using Cytoscape with ***,the expression pattern of core genes was analyzed using Western blot and immunohistochemistry in a carbon tetrachloride(CCl_(4))-induced liver cirrhosis mouse model and in patient liver ***,Kaplan-Meier curves were constructed using the Kaplan-Meier plotter online *** Forty-five DEGs(43 upregulated and 2 downregulated genes)associated with liver cirrhosis were identified from three GEO *** hub genes were identified,which were upregulated in liver *** blot and immunohistochemical analyses of the three core genes,decorin(DCN),dermatopontin(DPT),and SRY-box transcription factor 9(SOX9),revealed that they were highly expressed in the CCl4-induced liver cirrhosis mouse *** expression levels of DCN and SOX 9 were positively correlated with the degree of fibrosis,and SOX 9 level in HCC patients was significant

关键词： liver cirrhosis Hepatocellular carcinoma Bioinformatical analysis Decorin Dermatopontin SRY-box transcription factor 9

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Population genomics reveals that natural variation in PRDM16 contributes to cold tolerance in domestic cattle

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Zoological Research 2022年第2期43卷 275-284页

作者： Chun-Long Yan Jun lin yuan-yuan Huang Qing-Shan Gao Zheng-Yu Piao shou-li yuan li Chen Xue Ren Rong-Cai Ye Meng Dong Han-lin Zhang Hui-Qiao Zhou Xiao-Xiao Jiang Wan-Zhu Jin Xu-Ming Zhou Chang-Guo Yan College of Agriculture Yanbian UniversityYanjiJilin 133000China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China North-East Cold Region Beef Cattle Science&Technology Innovation Ministry of Education Engineering Research Center Yanbian UniversityYanjiJilin 133000China Annoroad Gene Technology Co.Ltd Beijing 100176China

Environmental temperature serves as a major driver of adaptive changes in wild *** discover the mechanisms underpinning cold tolerance in domestic animals,we sequenced the genomes of 28 cattle from warm and cold areas across *** characterizing the population structure and demographic history,we identified two genetic clusters,i.e.,northern and southern groups,as well as a common historic population peak at 30 kilo years *** scan of cold-tolerant breeds determined potential candidate genes in the thermogenesis-related pathways that were under ***,functional analysis identified a substitution of PRDM16(p.P779 L)in northern cattle,which maintains brown adipocyte formation by boosting thermogenesis-related gene expression,indicating a vital role of this gene in cold *** findings provide a basis for genetic variation in domestic cattle shaped by environmental temperature and highlight the role of reverse mutation in livestock species.

关键词： Population genomics Cattle Cold tolerance PRDM16 Brown adipose tissue

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Dihydroergotamine ameliorates liver fibrosis by targeting transforming growth factor β type Ⅱ receptor

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World Journal of Gastroenterology 2023年第20期29卷 3103-3118页

作者： Ke-Xin Zheng shou-li yuan Meng Dong Han-lin Zhang Xiao-Xiao Jiang Chun-Long Yan Rong-Cai Ye Hui-Qiao Zhou li Chen Rui Jiang Zi-Yu Cheng Zhi Zhang Qi Wang Wan-Zhu Jin Wen Xie Center of liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Graduate School University of the Chinese Academy of SciencesBeijing 100049China Graduate School Agriculture College of Yanbian UniversityYanji 133002Jilin ProvinceChina

BACKGROUND The transforming growth factor β(TGFβ) signaling pathway plays a crucial role in the development of liver fibrosis by activating TGFβ type Ⅱ receptor(TGFβR2), followed by the recruitment of TGFβR1 finally triggering downstream signaling *** To find drugs targeting TGFβR2 that inhibit TGFβR1/TGFβR2 complex formation, theoretically inhibit TGFβ signaling pathway, and thereby ameliorate liver *** Food and Drug Administration-approved drugs were screened for binding affinity with TGFβR2 by virtual molecular docking. We identified 6 candidates and further explored their potential by Cell Counting Kit-8(CCK-8) cell cytotoxic experiment to validate toxicity and titrated the best cellular working concentrations. Next, we further demonstrated the detailed molecular working mechanisms using mutagenesis analysis. Finally, we used a mouse model to investigate its potential anti-liver fibrosis *** We identified 6 drug candidates. Among these 6 drugs, dihydroergotamine(DHE) shows great ability in reducing fibrotic gene expressions such as collagen, p-SMAD3, and α-SMA in TGFβ induced cellular model of liver fibrosis in LX-2 cells. Furthermore, we demonstrated that DHE binds to TGFβR2. Moreover, mutation of Leu27, Phe30, Thr51, Ser52, Ile53, and Glu55 of TGFβR2 disrupted the binding of TGFβR2 with DHE. In addition, DHE significantly improved liver fibrosis, as evidenced by Masson’s trichrome staining of liver sections. This is further supported by the width and the velocity of the portal vein, and serum markers of liver function. In line with those observations, DHE also decreased macrophages infiltration and extracellular matrix deposition in the *** DHE alleviates liver fibrosis by binding to TGFβR2 thereby suppressing TGFβ signaling pathway. We show here that as far as drug repurposing, DHE has great potential to treat liver fibrosis.

关键词： liver fibrosis Transforming growth factorβ(TGFβ)signaling pathway TGFβtype II receptor(TGFβR2) Virtual screening Drug-repurposing Dihydroergotamine

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The design of intelligent marking system for electronic circuit experiment reports

The design of intelligent marking system for electronic circ...

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教育部中南地区高等学校电子电气基础课教学研究会第二十届学术年会

作者： Wang shou-hua,li yuan-qing,Huang Wen-li,Tan Sheng-liang (Guilin University of Electronic Technology,Guilin 541004)

Aimed at the multi-disciplinary teaching of electronic circuit experiment for school students,an intelligent marking system for electronic circuit experiment reports is *** structure and implementation of the system framework are described. The students can upload experiment data Lo the teacher’ PC by wireless sense method in this system,generating test reports and marking them *** students can upload the experiment data in the class,and download them after school.

关键词： Multi-disciplinary teaching and learning electronic circuit experiment reports wireless sense RF chip microcontroller

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试论细致服务在提升高校图书馆服务质量中的作用

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科技情报开发与经济 2012年第4期22卷 54-56页

作者：李寿媛南京特殊教育职业技术学院图书馆江苏南京210038

从充分发挥文献的作用、拉近与读者的距离、确立图书馆在学校的中心地位等方面论述了细致服务在提升高校图书馆服务质量中的重要性。

关键词：高校图书馆读者服务服务质量

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影响高校图书馆综合效益的隐性因素分析

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科技情报开发与经济 2012年第19期22卷 90-93页

作者：李寿媛南京特殊教育职业技术学院图书馆江苏南京210038

分析了当前高校图书馆中存在的影响其综合效益的隐性因素,并从加强宣传、完善设备和系统、强化知识和技能培训等方面进行了思考。

关键词：高校图书馆综合效益隐性因素

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Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng li Meifeng Cai Shengjun Miao yuan li Yu Wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

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如何界定监理责任

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工程质量 2006年第10期24卷 23-24页

作者：李守元枣庄市城市建设综合开发公司

在监督过程中发现显在或潜在的问题;发现问题后向有关单位提出问题。其实质是一种补充责任。

关键词：监理责任界定提出问题

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聋人大学生阅读状况调查与思考——以全国5所特殊教育高等院校为例

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科技情报开发与经济 2012年第23期22卷 31-34页

作者：李寿媛南京特殊教育职业技术学院图书馆江苏南京210038

通过对全国5所特殊教育高等院校聋人大学生课外阅读状况的抽样调查,分析了全国聋人大学生的阅读现状,总结了他们在阅读方面的特点,并提出了高校图书馆改进聋生服务的措施。

关键词：特殊教育高等院校高校图书馆聋人大学生课外阅读读者服务

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从经济学的角度浅谈高校学生资助效益问题

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阜阳师范学院学报（社会科学版） 2013年第4期 105-108页

作者：李守远阜阳师范学院体育学院安徽阜阳236037

高校家庭经济困难学生资助工作对贫困生顺利入学并完成学业,促进教育公平、社会公平具有重要的意义。2007年新资助政策体系的建立,进一步推动了高校资助工作的深入开展,取得了显著的资助效益。而在资助工作实际操作过程中却存在着贫困... 详细信息

高校家庭经济困难学生资助工作对贫困生顺利入学并完成学业,促进教育公平、社会公平具有重要的意义。2007年新资助政策体系的建立,进一步推动了高校资助工作的深入开展,取得了显著的资助效益。而在资助工作实际操作过程中却存在着贫困认定不准确、有偿资助和无偿资助比例失调和资助资金使用不合理的现象。针对这些现象从经济学角度分析其原因,提出贫困认定相关各方权责清晰、调整比例和加强激励约束机制建设的对策建议,以期实现资助资金的最大效益。

关键词：高校学生资助经济学效益

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