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Journal of Materials Science & Technology 2020年第24期59卷 195-202页

作者： Kyungeun Jung Du Hyeon Kim Jaemin Kim Sunglim Ko Jae Won Choi Ki Chul Kim Sang-Geul Lee man-Jong Lee Department of Advanced Technology fusion Konkuk UniversitySeoul05029Republic of Korea a Department of Chemistry Konkuk UniversitySeoul05029Republic of Korea Department of Mechanical Design and Production Engineering Konkuk UniversitySeoul05029Republic of Korea Division of Chemical Engineering Konkuk UniversitySeoul05029Republic of Korea Daegu Center Korea Basic Science InstituteDaegu41566Republic of Korea

The effect of ultraviolet-ozone(UVO)irradiation on amorphous(am)SnO_(2) and its impact on the photoconversion efficiency of MAPbI3-based perovskite solar cells were investigated in *** treatment was found to increase the amount of chemisorbed oxygen on the am-SnO_(2) surface,reducing the surface energy and contact *** changes in the am-SnO_(2) surface lowered the Gibbs free energy for the densification of perovskite films and facilitated the formation of homogeneous perovskite *** addition,the fermi energy of the UVO-treated am-SnO_(2) shifted upwards to achieve an ideal band offset for MAPbI3,which was verified by theoretical calculations based on the density functional *** achieved a champion efficiency of 19.01% with a statistical reproducibility of 17.01±1.34% owing to improved perovskite film densification and enhanced charge transport/extraction,which is considerably higher than the 13.78±2.15% of the ***,UVO-treated,am-SnO_(2)-based devices showed improved stability and less hysteresis,which is encouraging for the future application of up-scaled perovskite solar cells.

关键词： UV-ozone Amorphous SnO_(2) Electron selective layers Planar perovskite solar cells Hysteresis

THE DYNAMIC MONITORING Of HORQIN SAND LAND USING REMOTE SENSING

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Chinese Geographical Science 2002年第3期12卷 238-243页

作者： HU Yuan-man JIANG Yan CHANG Yu BU R en-cang LI Yue-hui XU Chong-gang Institute of Applied Ecology the Chinese Academy of SciencesShenyang110016P.R.China

Horqin Sand Land is regarded as the typical region for studying the problemof desertification. The integration of 3S(GIS, GPS and RS) techniques offer a roost helpful methodto study and monitor the dynamics of desertification. Based on the data derived from 3 periods'multitemporal Landsat TM imagery of the 1990s, the regional land use and dynamics of desertificationin Horqin Sand Land were studied. The main results revealed that: 1) as long as the general changetendency was concerned, the desertification of Horqin Sand Land would continue to spread; 2) therewas a gradual decrease in the area of both moving sand dunes and semi-stabilized ones, which meantthat fruitful progress had been made to control the desertification during the 1990s; 3) as a resultof unreasonable cultivation, the total area of stabilized sand dunes and grassland in the middleand western region decreased obviously. It suggested that the increasing damage caused by human wasleading to the hazard of further desertification. So in the future, it is necessary to take moreeffective measures to control the spread of desertification and restore the degraded ecosystems forthe purpose of optimizing the global eco-environment in Horqin Sand Land.

关键词： horqin sand land desertification remote sensing dynamic monitoring

Phosphorylation of sarcoplasmic and myofibrillar proteins in three ovine muscles during postmortem ageing

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Journal of Integrative Agriculture 2019年第7期18卷 1643-1651页

作者： WANG Ying LI Xin LI Zheng DU man-ting ZHU Jie ZHANG She-qi ZHANG De-quan Laboratory of Biomechanics and Engineering Institute of Biophysics and College of ScienceNorthwest A&F UniversityYangling 712100P.R. China Institute of food Science and Technology Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products ProcessingMinistry of AgricultureBeijing 100193P.R. China

This study aimed to examine changes in phosphorylation of sarcoplasmic and myofibrillar proteins from longissimus lumborum,semitendinosus,and psoas major muscles during postmortem ageing for 5 *** sarcoplasmic and myofibrillar proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with phosphorous and protein specific *** fragmentation index,pH,the content of lactic acid and the relative activity of μ-calpain in three ovine muscles were *** results showed that the relative phosphorylation level of sarcoplasmic and myofibrillar proteins of psoas major muscle were lower compared with longissimus lumborum and semitendinosus muscles(Pf psoas major muscle was the lowest at 0.5 h postmortem,and the highest after 12 h postmortem(Pf μ-calpain was higher within 5 d postmortem and myofibril fragmentation index was higher after 1 d postmortem in psoas major muscle than those of longissimus lumborum and semitendinosus muscles(Pf pH decline to influence the μ-calpain activity and then proteolysis of proteins *** study gives a new perspective of the mechanism of postmortem meat tenderization.

关键词： ovine muscle protein phosphorylation μ-calpain myofibril fragmentation index

Synthesis and Crystal Structure of a Novel manganese Coordination Polymer: 2,5-Bis(4-pyridyl)-3,4-diaza-2,4-hexadiene

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Chinese Journal of Structural Chemistry 2007年第2期26卷 219-223页

作者： CHEN man-Sheng DENG Yi-fang ZHANG Chun-Hua KUANG Dai-Zhi fENG Yong-Lan PENG Yun-Lin Department of Chemistry and Materials Science Hen gyan g Normal University Hen gyan g 421008 China

A new manganese coordination polymer 1, [Mn（H2O）4（L）]n（ClO4）2n.2nH2O.3nL （L = 2,5-bis（4-pyridyl）-3,4-diaza-2,4-hexadiene）, has been synthesized and structurally characterized. The crystal structure was determined by single-crystal X-ray diffraction. The crystal is of monoclinic, space group Pn with a = 13.9176（8）, b = 15.4754（9）, c = 15.9670（9） A, β = 99.5010（10）°, V = 3391.8（3） ]k3, Z = 2, C56H68C12MnN16O14, Mr= 1315.10, Dc = 1.288 g/cm^3,μ = 0.344 mm^-1, f（000） = 1374, R = 0.0733 and wR = 0.2035. In the crystal the manganese atom is six-coordinated by two nitrogen atoms from 2,5-bis（4-pyridyl）-3,4-diaza-2,4-hexadiene and four oxygen atoms from water molecules, completing an octahedral geometry. The title complex exhibits a novel supramolecular layer architecture sustained by the concurrence of coordination bonds, hydrogen bonds, and π-π stacking interactions.

关键词： manganese complex Schiff bases crystal structure

3-Aminopropyltriethoxysilane Complexation with Iron Ion Modified Anode in Marine Sediment Microbial fuel Cells with Enhanced Electrochemical Performance

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Journal of Ocean University of China 2021年第3期20卷 581-589页

作者： ZAI Xuerong GUO man HAO Yaokang HOU Shaoxin YANG Zhiwei LI Jia LI Yang JI Hongwei fU Yubin College of Chemistry and Chemical Engineering the Key Laboratory of Marine Chemistry Theory and TechnologyMinistry of EducationOcean University of ChinaQingdao 266100China School of Materials Science and Engineering Ocean University of ChinaQingdao 266100China

Anode modification plays a key role in higher power output in marine sediment microbial fuel cells(MSMfCs).A low-molecular organosilicon compound(3-aminopropyltriethoxysilane)was grafted onto the surface of carbon felt using chemical method and a composite modified anode was prepared through organic ligands coordination fe^(3+)for better electro-chemical *** show that the biofilm resistance of the composite modified anode(2707Ω)is 1.3 times greater than that of the unmodified anode(2100Ω),and its biofilm capacitance also increases by 2.2 times,indicating that the composite modification pro-motes the growth and attachment of electroactive bacteria on the *** specific capacitance(887.8 fm^(−2))is 3.7 times higher than that of unmodified anode,generating a maximum current density of 1.5Am^(−2).In their Tafel curves,the composite modified anodic exchange current density(5.25×10^(−6)Acm^(−2))is 5.8 times bigger than that of unmodified anode,which suggests that the electro-chemical activity of redox,anti-polarization ability and electron transfer kinetic activity are significantly *** marine sediment microbial fuel cell with the composite modified anode generates the higher power densities than the blank(203.8mWm^(−2) versus 45.07mWm^(−2)),and its current also increases by 4.4 *** free amino groups on the anode surface expands a creative idea that the modified anode ligates the natural fe(Ⅲ)ion in sea water in the MSMfCs for its higher power output.

关键词： 3-aminopropyltriethoxysilane iron ion composite modified carbon anode electro-chemical performances marine sediment microbial fuel cells

Patterns of Genetic Diversity and Population Structure of the Clonal Herb, Potentilla fragarioides var. sprengeliana (Rosaceae) in Korea

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Acta Botanica Sinica 2000年第1期42卷 64-70页

作者： man-Kyu HUH Hong-Wook HUH Department of Biology Education Pusan National University The Republic of Korea 609-735

The genetic diversity and population structure of Potentilla fragarioides var. sprengeliana (Rosaceae) in Korea were investigated using genetic variation at 22 allozyme loci. The percent of polymorphic loci within the enzymes was 59.1%. The genetic diversity at the species level and at the population level was high ( H es =0.210; H ep =0.199, respectively), whereas the extent of the population divergence was relatively low ( G ST =0.074). f IS , a measure of the deviation from random mating within the 19 populations, was 0.331 . An indirect estimate of the number of migrants per generation ( N m =3.15) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a slight heterozygote deficiency in some populations and at some loci. The mean genetic identity between populations was 0.985. It is highly probable that the trend of genetic uniformity in a relatively homogeneous habitat is thought to be operated among Korean populations of P. fragarioides var. sprengeliana .

关键词： genetic diversity population structure Potentilla fragarioides var. sprengeliana

Hydrogen isotopic replacement and microstructure evolution in zirconium deuteride implanted by 150 keV protons

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Chinese Physics B 2021年第10期30卷 85-91页

作者： man Zhao Mingxu Zhang Tao Wang Jiangtao Zhao Pan Dong Zhen Yang Tieshan Wang School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China Institute of fluid Physics CAEPMianyang 621900China Sino-french Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China

Zirconium tritiated(ZrTx)is an alternative target material for deuteron-triton(D-T)reaction neutron *** isotopic replacement and microstructure evolution induced by hydrogen isotope implantation could significantly affect the performance of the target *** this work,the zirconium deuteride film deposited on Mo/Si substrate was implanted by 150 keV protons with fluence from 1×10^(16 ) to 1×10^(18 )protons/cm^(2).After implantation,the depth profiles of retained hydrogen(H)and deuterium(D)in these target films were analyzed by elastic recoil detection analysis(ERDA),and time of flight-secondary ion mass spectrometry(Tof-SIMS).Additionally,the microstructure evolution was also observed by x-ray diffraction(XRD)and scanning electron microscope(SEM).The D concentration in the ZrDx film decreased versus the proton implantation *** analytical model was proposed to describe the hydrogen isotopic trapping and exchange as functions of incident protons ***,the XRD analysis revealed that no new phase was formed after proton ***,circular flakings were observed on the ZrDx surface from SEM images at fluence up to 1×10^(18) protons/cm^(2),and this surface morphology was considered to associate with the hydrogen atoms congregation in Mo/Si boundary.

关键词： zirconium deuterides isotopic replacement proton implantation microstructure evolution

Expression and reconstitution of the bioluminescent Ca2+reporter aequorin in human embryonic stem cells,and exploration of the presence of functional IP3 and ryanodine receptors during the early stages of their differentiation into cardiomyocytes

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Science China(Life Sciences) 2016年第8期59卷 811-824页

作者： Harvey Y.S.Chan man Chun Cheung Yi Gao Andrew L.Miller Sarah E.Webb Division of Life Science&State Key Laboratory of Molecular Neuroscience HKUSTClear Water BayHong KongChina Stem Cell&Regenerative Medicine Consortium Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong KongChina Marine Biological Laboratory Woods Hole MA 02543USA

In order to develop a novel method of visualizing possible Ca2＋ signaling during the early differentiation of hESCs into cardi- omyocytes and avoid some of the inherent problems associated with using fluorescent reporters, we expressed the biolumines- cent Ca2＋ reporter, apo-aequorin, in HES2 cells and then reconstituted active holo-aequorin by incubation withf-coelenterazine. The temporal nature of the Ca2＋ signals generated by the holo-f-aequorin-expressing HES2 cells during the earliest stages of differentiation into cardiomyocytes was then investigated. Our data show that no endogenous Ca2＋ transients （generated by re- lease from intracellular stores） were detected in 1-12-day-old cardiospheres but transients were generated in cardiospheres following stimulation with KC1 or CaC12, indicating that holo-f-aequorin was functional in these cells. furthermore, following the addition of exogenous ATP, an inositol trisphosphate receptor （IP3R） agonist, small Ca2＋transients were generated from day 1 onward. That ATP was inducing Ca2＋ release from functional IP3Rs was demonstrated by treatment with 2-APB, a known IP3R antagonist. In contrast, following treatment with caffeine, a ryanodine receptor （RyR） agonist, a minima/Ca2＋ response was observed at day 8 of differentiation only. Thus, our data indicate that unlike RyRs, IP3Rs are present and continually functional at these early stages of cardiomyocyte differentiation.

关键词： Ca2+ signaling apo-aequorin expression bioluminescence HES2 human embryonic stem cells hESC-derived cardiospheres IP3 and ryanodine receptors

Genetic Analysis and Molecular Mapping of a Rolling Leaf Mutation Gene in Rice

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Journal of Integrative Plant Biology 2007年第12期49卷 1746-1753页

作者： Ji-Cai Yi Chu-Xiong Zhuang Xu-Jie Wang You-Pei Cao Yao-Guang Liu man-Tong Mei Key Laboratory of Plant functional Genomics and Biotechnology of Guangdong Province College of Life Science South China Agricultural University Guangzhou 510642 China

A rice mutant with rolling leaf, namely γ-rl, was obtained from M2 progenies of a native indica rice stable strain Qinghuazhan （QHZ） from mutagenesis of dry seeds by γ-rays. Genetic analysis using the f2 population from a cross between this mutant and QHZ indicated the mutation was controlled by a single recessive gene. In order to map the locus for this mutation, another f2 population with 601 rolling leaf plants was constructed from a cross between y-rl and a japonica cultivar 02428. After primary mapping with SSR （simple sequence repeats） markers, the mutated locus was located at the short arm of chromosome 3, flanked by RM6829 and RM3126. A number of SSR, InDel （insertion/deletion） and SNP （single nucleotide polymorphism） markers within this region were further developed for fine mapping. finally, two markers, SNP121679 and InDe1422395, were identified to be flanked to this locus with genetic distances of 0.08 cM and 0.17 cM respectively, and two SNP markers, SNP75346 and SNPl10263, were found to be co-segregated with this locus. These results suggested that this locus was distinguished from all loci for the rolling leaf mutation in rice reported so far, and thus renamed rl10（t）. By searching the rice genome database with closely linked markers using BLAST programs, an e-physical map covering rl10（t） locus spanning about a 50 kb region was constructed. Expression analysis of the genes predicted in this region showed that a gene encoding putative flavin-containing monooxygenase （fMO） was silenced in γ-rl, thus this is the most likely candidate responsible for the rolling leaf mutation.

关键词： fine mapping genetic analysis rice rolling leaf mutant.

Quantification of Panax notoginseng saponins metabolites in rat plasma with in vivo gut microbiota-mediated biotransformation by HPLC-MS/MS

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Chinese Journal of Natural Medicines 2019年第3期17卷 231-240页

作者： GUO Yin-Ping CHEN man-Yun SHAO Li ZHANG Wei RAO Tai ZHOU Hong-Hao HUANG Wei-Hua Department of Clinical Pharmacology Xiangya Hospital Central South University Institute of Clinical Pharmacology Hunan Key Laboratory of Pharmacogenetics Central South University Department of Pharmacognosy School of Pharmacy Hunan University of Chinese Medicine

Panax notoginseng saponins(PNS) are the major components of Panax notoginseng, with multiple pharmacological activities but poor oral bioavailability. PNS could be metabolized by gut microbiota in vitro, while the exact role of gut microbiota of PNS metabolism in vivo remains poorly understood. In this study, pseudo germ-free rat models were constructed by using broad-spectrum antibiotics to validate the gut microbiota-mediated transformation of PNS in vivo. Moreover, a high performance liquid chromatography-electrospray ionization tandem mass spectrometry(HPLC-ESI-MS/MS) was developed for quantitative analysis of four metabolites of PNS, including ginsenoside f1(Gf1), ginsenoside Rh2(GRh2), ginsenoside compound K(GCK) and protopanaxatriol(PPT). The results showed that the four metabolites could be detected in the control rat plasma, while they could not be determined in pseudo germ-free rat plasma. The results implied that PNS could not be biotransformed effectively when gut microbiota was disrupted. In conclusion, gut microbiota plays an important role in biotransformation of PNS into metabolites in vivo.

关键词： Panax notoginseng Gut microbiota HPLC-MS/MS Saponins Ginsenoside compound K Protopanaxatriol