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球茎甘蓝qRT-PCR内参基因的筛选及稳定性验证

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甘肃农业大学学报 2024年第1期59卷 144-152页

作者：郭怡婷孙世英赵文菊王鑫淼李晓娟马一栋任延靖青海大学青海西宁810016 青海大学农林科学院青藏高原种质资源研究与利用实验室青海西宁810016 青海大学三江源生态和高原农牧业国家重点实验室青海西宁810016

【目的】通过对已知内参基因进行筛选,确定球茎甘蓝最合适的内参基因,确保实时荧光定量PCR(qRT-PCR)的准确表达。【方法】本试验选择了11种参考基因,利用GeNorm、Normalfinder和Bestkeep软件对紫色球茎甘蓝和绿色球茎甘蓝不同部位的内... 详细信息

【目的】通过对已知内参基因进行筛选,确定球茎甘蓝最合适的内参基因,确保实时荧光定量PCR(qRT-PCR)的准确表达。【方法】本试验选择了11种参考基因,利用GeNorm、Normalfinder和Bestkeep软件对紫色球茎甘蓝和绿色球茎甘蓝不同部位的内部参考基因进行了分析。【结果】3个分析结果显示,内参基因Tip41在球茎甘蓝不同组织部位表达最稳定。【结论】Tip41是作为内参基因的最佳选择,为球茎甘蓝后续的分子生物学相关研究提供基础。

关键词：球茎甘蓝内参基因荧光定量

维普期刊数据库博看期刊

Some Properties of the g-Good-Neighbor (g-Extra) Diagnosability of a Multiprocessor System

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American Journal of Computational Mathematics 2016年第3期6卷 259-266页

作者： Yunxia ren shiying Wang School of Mathematics and Information Science Henan Normal University Xinxiang China

Diagnosability of a multiprocessor system is one important study topic. In 2012, Peng et al. proposed a measure for fault tolerance of the system, which is called the g-good-neighbor diagnosability that restrains every fault-free node containing at least g fault-free neighbors. In 2015, Zhang et al. proposed a measure for fault diagnosis of the system, namely, g-extra diagnosability, which restrains that every fault-free component has at least g+1 fault-free nodes. In this paper, we obtain some properties of the g-good-neighbor (g-extra) diagnosability of the system and give the g-good-neighbor (g-extra) diagnosability of some graphs under the PMC model and MM* model.

关键词： Interconnection Network Combinatorics Diagnosability

A Proof for g-Good-Neighbor Diagnosability of Exchanged Hypercubes

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American Journal of Computational Mathematics 2019年第3期9卷 133-142页

作者： Yunxia ren shiying Wang School of Mathematics and Information Science Henan Normal University Xinxiang China

The diagnosability of a multiprocessor system or an interconnection network is an important research topic. The system and an interconnection network have an underlying topology, which is usually presented by a graph. In this paper, we show proof for the g-good-neighbor diagnosability of the exchanged hypercube EH (s,t) under the PMC model and MM* model.

关键词： Interconnection Network Diagnosability Exchanged Hypercube

The 2-Extra Diagnosability of Alternating Group Graphs under the PMC Model and MM* Model

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American Journal of Computational Mathematics 2018年第1期8卷 42-54页

作者： shiying Wang Yunxia ren School of Mathematics and Information Science Henan Normal University Xinxiang China

Diagnosability of a multiprocessor system is one important study topic. In 2015, Zhang et al. proposed a new measure for fault diagnosis of the system, namely, g-extra diagnosability, which restrains that every fault-free component has at least (g + 1) fault-free nodes. As a favorable topology structure of interconnection networks, the n-dimensional alternating group graph AGn has many good properties. In this paper, we give that the 2-extra diagnosability of AGn is 6n - 17 for n≥ 5 under the PMC model and MM* model.

关键词： Interconnection Network Diagnosability Alternating Group Graph

The Tightly Super 3-Extra Connectivity and Diagnosability of Locally Twisted Cubes

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American Journal of Computational Mathematics 2017年第2期7卷 127-144页

作者： Mujiangshan Wang Yunxia ren Yuqing Lin shiying Wang School of Electrical Engineering and Computer Science The University of Newcastle Newcastle NSW Australia School of Mathematics and Information Science Henan Normal University Xinxiang Henan China

Diagnosability of a multiprocessor system G is one important measure of the reliability of interconnection networks. In 2016, Zhang et al. proposed the g-extra diagnosability of G, which restrains that every component of G – S has at least (g + 1) vertices. The locally twisted cube LTQn is applied widely. In this paper, we show that LTQn is tightly (4n – 9) super 3-extra connected for n ≥ 6 and the 3-extra diagnosability of LTQn under the PMC model and MM* model is 4n - 6 for n ≥ 5 and n ≥ 7, respectively.

关键词： Interconnection Network Combinatorics Diagnosability

内蒙古东部区玉米草谷比与秸秆可收集系数监测

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中国沼气 2024年第4期42卷 53-56页

作者：赵靖丹冯志国任致贤李纯燕杨世英李晓包菡田晓燕张娟内蒙古自治区农牧业生态与资源保护中心内蒙古呼和浩特010021 内蒙古自治区水利工程建设质量与安全监督服务中心内蒙古呼和浩特010021

为丰富内蒙古东部区玉米草谷比与可收集系数测算样本数量,进一步完善秸秆资源台账数据,科学测算玉米秸秆资源量,在内蒙古东部区开展玉米草谷比与秸秆可收集系数监测试验。通过对呼伦贝尔市阿荣旗、兴安盟扎赉特旗、通辽市科左中旗、赤... 详细信息

为丰富内蒙古东部区玉米草谷比与可收集系数测算样本数量,进一步完善秸秆资源台账数据,科学测算玉米秸秆资源量,在内蒙古东部区开展玉米草谷比与秸秆可收集系数监测试验。通过对呼伦贝尔市阿荣旗、兴安盟扎赉特旗、通辽市科左中旗、赤峰市阿鲁科尔沁旗等4个旗县20个行政村12个苏木乡镇进行监测试验,结果表明,实测玉米草谷比为1.06~1.49,平均值为1.25;玉米秸秆可收集系数为0.78~0.86,平均值为0.82,数据可为内蒙古东部区秸秆资源化利用提供数据支撑。

关键词：内蒙古东部区玉米草谷比可收集系数监测

MSALL strategy for comprehensive and quantitative analysis of PEG and PEGylated doxorubicin by LC-high resolution Q-Q-TOF mass spectrometry and its application to pharmacokinetics study of MPEG550-DOX,MPEG2K-DOX and MPEG10K-DOX in rats

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MSALL strategy for comprehensive and quantitative analysis o...

应用多导睡眠图监测结果分析急性缺血性脑卒中患者早期睡眠障碍类型

第三届全国药物代谢动力学青年科学家论坛暨刘昌孝人才奖颁奖大会

作者： Lei Yin Tianming ren shiying Zhao Jingkai Gu Research Center for Drug Metabolism School of Life ScienceJilin University Research Institute of Translational Medicine The First Hospital of Jilin University

Aim:Establish and develop an LC-Q-Q-TOF MS/MS method based on MSALL technique for comprehensive and quantitative analysis of PEG conjugates and released compounds in biological samples and its application to a pharmacokinetic study of MPEG550-DOX,MPEG2 K-DOX and MPEG10 K-DOX in rats..Methods:Due to the complex structure and diverse physiochemical property of PEG,quantification analysis of PEG and PEGylated molecular agents in biological matrix presented many challenges in terms of assay sensitivity,selectivity,and *** this study,a data-independent acquisition method,MSALL-based approach using electrospray ionization(ESI) coupled quadrupole time of flight mass spectrometry(MS),was firstly utilized for the quantitative analysis of PEGylated pharmaceuticals in complex biological *** one injection,MSALL technique consists of a Q1 mass window through all the precursor ions,fragmenting in Q2(collision cell) and recording precursor ions(at low CE) and product ions(at high CE) with different ***:PEGylated doxorubicin was used as a representative compound of PEGylated drugs for this *** doxorubicin underwent dissociation in the second Quadrupole to generate a series of high resolution PEG related ions at m/z 89.0611,133.0869,177.1102,221.1366,265.1622,309.1878,353.2108 of various number repeating ethylene oxide subunits and compound related ions at m/z 321.0838,361.0785,***2 K-doxorubicin specific ions(at m/z 365.0735) have the better signal to noise ratio compared with the PEG related ions,which is more applicable when the chromatographic behavior of PEG and PEGylated small molecules is *** and PEGylated small molecules were detected by positive ion electrospray ionization followed by the high resolution extracted ions based on PEG and the specific *** method was successfully applied to pharmacokinetic study of MPEG550-DOX,MPEG2 K-DOX and MPEG10 K-DOX in rats after vein intravenous injection of PE

关键词： LC-MS/MS MSALL Q-Q-TOF

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中国健康心理学杂志 2024年第4期32卷 558-562页

作者：米唤金刘斌邓春颖马原源王雅楠任伯李世英毛文静华北理工大学附属医院神经内一科河北唐山063000

目的:应用多导睡眠图监测(polysomnography,PSG)结果客观分析急性缺血性脑卒中(Acute ischemic stroke,AIS)患者早期睡眠障碍特征,了解急性缺血性脑卒中患者早期睡眠障碍的类型,为临床干预提供依据。方法:选取住院的急性缺血性脑卒中患... 详细信息

目的:应用多导睡眠图监测(polysomnography,PSG)结果客观分析急性缺血性脑卒中(Acute ischemic stroke,AIS)患者早期睡眠障碍特征,了解急性缺血性脑卒中患者早期睡眠障碍的类型,为临床干预提供依据。方法:选取住院的急性缺血性脑卒中患者96例,入院后3天内完成匹兹堡睡眠质量指数量表(Pittsburgh Sleep Quality Index,PSQI)评估患者睡眠情况,根据PSQI量表评分标准及结果分为睡眠障碍组(n=60)和非睡眠障碍组(n=36),两组患者均进行整夜PSG监测,记录相关数据。比较两组患者一般资料及PSG相关睡眠参数。结果:1两组之间睡眠进程参数比较,睡眠障碍组的总睡眠时间及REM潜伏期均低于非睡眠障碍组(t=7.168,7.658;P<0.05),而睡眠潜伏期明显高于非睡眠障碍组(t=-7.161,P<0.05);2两组之间睡眠质量参数比较,睡眠障碍组的觉醒次数及觉醒指数均高于非睡眠障碍组(t=-11.728,-7.766;P<0.05),而睡眠效率明显低于非睡眠障碍组(t=5.684,P<0.05);3两组之间睡眠结构参数比较,睡眠障碍组的N1期及N2期均高于非睡眠障碍组(t=-7.147,-8.485;P<0.05),而REM期及N3期低于非睡眠障碍组(t=9.624,9.842;P<0.05);④两组之间睡眠呼吸事件比较,睡眠障碍组的AHI、仰卧位AHI、低通气指数均高于非睡眠障碍组(t=-7.618,-10.086,-11.185;P<0.05),而LSaO2低于非睡眠障碍组(t=8.098,P<0.05)。结论:急性缺血性脑卒中患者早期存在明显的睡眠进程异常、睡眠质量下降、睡眠结构紊乱及睡眠呼吸事件方面的异常。应用PSG正确评估急性缺血性脑卒中患者早期睡眠障碍的类型并进行干预,有助于改善患者的预后。

关键词：急性缺血性脑卒中睡眠障碍多导睡眠图监测匹兹堡睡眠质量指数

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星图网络的定向图

河南师范大学学报（自然科学版） 2014年第4期42卷 1-5页

作者：任云霞王世英河南师范大学数学与信息科学学院

主要研究了星图网络Sn的定向图.证明了如下结论:对于非负整数a和b,若存在满足每个顶点的入度或者是a或者是b的一个Sn的定向图,则存在非负整数s和t满足方程s+t=n!和as+bt=(n-1)/2.进一步,对于满足特定条件的非负整数a,b和n,存在Sn的定向... 详细信息

主要研究了星图网络Sn的定向图.证明了如下结论:对于非负整数a和b,若存在满足每个顶点的入度或者是a或者是b的一个Sn的定向图,则存在非负整数s和t满足方程s+t=n!和as+bt=(n-1)/2.进一步,对于满足特定条件的非负整数a,b和n,存在Sn的定向图使得每个顶点的入度或者是a或者是b.

关键词：互连网络星图网络定向入度