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数字化背景下博物馆文物传播的创新与发展

文化创新比较研究 2024年第10期8卷 96-100页

作者：叶诗琪陈伟福建江夏学院国际教育学院福建福州350108 福建江夏学院金融学院福建福州350108

数字化为博物馆文物传播的创新与发展提供了新动力。该文在系统梳理我国博物馆文物传播“以物为本”“线上线下”“数字化转型”三大阶段实践历程的基础上,剖析数字化对博物馆文物传播模式、传播主体、传播内容、传播功能的具体影响。... 详细信息

数字化为博物馆文物传播的创新与发展提供了新动力。该文在系统梳理我国博物馆文物传播“以物为本”“线上线下”“数字化转型”三大阶段实践历程的基础上,剖析数字化对博物馆文物传播模式、传播主体、传播内容、传播功能的具体影响。发现数字化背景下博物馆的文物传播存在复合型人才偏少、数字化转型资金使用效率过低、数字化建设物力支撑不足等内部阻碍,以及欠发达地区数字化技术运用不足、文物传播受众偏好差异大等外部阻碍,并据此提出针对性发展路径,即内培外引、市场驱动、上下联动、受众导向等,以此提升博物馆文物传播效率,更好地担负起增强人民群众文化自信的使命。

关键词：数字化博物馆文物传播传播模式传播主体传播内容传播功能

Efficient genome editing in rice with miniature Cas12f variants

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aBIOTECH 2024年第2期5卷 184-188页

作者： Zhengyan ye Yuanyan Zhang shiqi He Shaokang Li Longjiong Luo Yanbiao Zhou Junjie Tan Jianmin Wan Sanya Institute of Nanjing Agricultural University State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationProvince and Ministry Co-sponsored Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China Zhongshan Biological Breeding Laboratory No.50 Zhongling StreetNanjing 210014China Key Laboratory of Southern Rice Innovation&Improvement Ministry of Agriculture and Rural Affairs/Hunan Engineering Laboratory of Disease and Pest Resistant Rice BreedingYuan Longping High-Tech Agriculture Co.LtdChangsha 410001China

Genome editing,particularly using the CRISPR/Cas system,has revolutionized biological research and crop *** the widespread use of CRISPR/Cas9,it faces limitations such as PAM sequence requirements and challenges in delivering its large protein into plant *** hypercompact Cas12f,derived from Acidibacillus sulfuroxidans(AsCas12f),stands out due to its small size of only 422 amino acids and its preference for a T-rich motif,presenting advantageous features over ***,its editing efficiency is extremely low in *** studies have generated two AsCas12f variants,AsCas12f-YHAM and AsCas12f-HKRA,demonstrating higher editing efficiencies in mammalian cells,yet their performance in plants remains *** this study,through a systematic investigation of genome cleavage activity in rice,we unveiled a substantial enhancement in editing efficiency for both AsCas12f variants,particularly for AsCas12f-HKRA,which achieved an editing efficiency of up to 53%.Furthermore,our analysis revealed that AsCas12f predominantly induces deletion in the target DNA,displaying a unique deletion pattern primarily concentrated at positions 12,13,23,and 24,resulting in deletion size mainly of 10 and 11 bp,suggesting significant potential for targeted DNA deletion using *** findings expand the toolbox for efficient genome editing in plants,offering promising prospects for precise genetic modifications in agriculture.

关键词： CRISPR/Cas Cas12 AsCas12f Rice Genome editing

Pairs of thiol-substituted 1,2,4-triazole-based isomeric covalent inhibitors with tunable reactivity and selectivity

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Chinese Chemical Letters 2024年第7期35卷 329-335页

作者： shiqi Xu Zi ye Shuang Shang Fengge Wang Huan Zhang Lianguo Chen Hao Lin Chen Chen Fang Hua Chong-Jing Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of Active Substance Discovery and Druggability EvaluationInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study(BZ0150)Institute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100050China The First Affiliated Hospital of Wenzhou Medical University Wenzhou 325000China

Covalent bioactive compounds are successfully used in clinic and attracted intense research efforts in the fundamental study as well as drug *** advantageous effects of covalent compounds compared with non-covalent ones are highly dependent on electrophilic ***,electrophilic warheads with tunable reactivity and selectivity are highly demanded in fields of medicinal chemistry and chemical ***,we report a novel electrophilic warhead,chloromethyl group activated by thiol-substituted 1,2,***,a pair of regioisomers could be simultaneously occurred in the step of alkylation during the synthesis of this unique *** is a rare example that the alkylation could simultaneously generate these two separable regioisomers of 1,2,4-triazole at the nitrogen or sulfur *** covalent-working mechanism of this new warhead is confirmed by various chemoproteomics experiments including target identification and binding site ***,the reactivity and selectivity of this new electrophilic warhead could be efficiently tuned by virtue of stereo ***,one pair of regioisomers(19S and 19X)induced distinct modes of cell *** 19S could induce apoptosis of colon cancer cells while 19X could induce both apoptosis and ***,this study provides pairs of novel electrophilic warheads that could be useful not only in supporting the design of covalent compounds for drug discovery but also in providing chemical probes for the fundamental biological study.

关键词： Electrophilic warhead Covalent inhibitor 1,2,4-Triazole Chemical proteomics GSTO1

Research on Traveling Routes Problems Based on Improved Ant Colony Algorithm

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Communications and Network 2013年第3期5卷 606-610页

作者： Zhanchang Yu Sijia Zhang Siyong Chen Bingxing Liu shiqi ye Department of Information Science and Technology Jinan University Guangzhou China

This paper studies how to obtain a reasonable traveling route among given attractions. Toward this purpose, we propose an objective optimization model of routes choosing, which is based on the improved Ant Colony Algorithm. Furthermore, we make some adjustment in parameters in order to improve the precision of this algorithm. For example, the inspired factor has been changed to get better results. Also, the ways of searching have been adjusted so that the traveling routes will be well designed to achieve optimal effects. At last, we select a series of attractions in Beijing as data to do an experimental analysis, which comes out with an optimum route arrangement for the travelers;that is to say, the models we propose and the algorithm we improved are reasonable and effective.

关键词： Traveling Routes Ant Colony Algorithm Parameter Adjustment Searching Ways

Induction of Cytochrome P450 4A14 is associated with Angiotensin–induced Renal Fibrosis in Mice

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Induction of Cytochrome P450 4A14 is associated with Angiote...

Induction of Cytochrome P450 4A14 is associated with Angiotensin Ⅱ-induced Renal Fibrosis in Mice

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：周云枫 Min Gao Shizheng Huang shiqi ye Youfei Guan 深圳大学生理系

Angiotensin Ⅱ(Ang Ⅱ)plays an important role in the pathogenesis of hypertension and its associated cardiovascular and renal *** elucidate the molecular mechanism by which Ang Ⅱ induces renal damage,we determined expression profile of genes involved in arachidonic acid metabolism in the kidneys of mice receiving chronic Ang Ⅱ infusion for 14 *** P450 4A14(CYP4A14)was found to be significantly induced by Ang Ⅱ,which was associated with a marked increase in systolic blood pressure and ***-time PCR,Western blot and immunohistochemistry assays demonstrated that CYP4A14 expression was predominantly upregulated in the proximal tubules of Ang Ⅱ-infused *** production of the major CYP4A14 metabolite,20-hydroxyeicosatetraenoic acid(20-HETE),was also significantly increased in the Ang Ⅱ-treated *** to wild-type(WT)mice,CYP4A14-/-mice exhibited significantly lower levels of blood pressure and proteinuria following Ang Ⅱ *** Ⅱ infusion resulted in renal glomeruloscelerosis and tubulointerstitial fibrosis in WT mice,which was significantly attenuated in CYP4A14-/-***,renal collagen I levels were significantly lower in CYP4A14-/-mice than in WT mice following Ang Ⅱ ***,Ang Ⅱ-induced renal fibrosis was associated with elevated expression of renal proinflammatory genes including MCP-1,TNF-α,IL-1,IL-6 and F4/80,whose expression was significantly decreased in CYP4A14-/-***,treatment of proximal tubule cells(PTCs)with Ang Ⅱ significantly induced CYP4A14 expression and20-HETE *** Ang Ⅱ and 20-HETE treatment resulted in a marked increase in TGF-βand collagen *** addition,elevated CYP4A14 expression in Ang Ⅱ-treated PTCs was blocked by ERK,JNK and p38 ***,these findings suggest that Ang Ⅱ-mediated renal fibrosis may result from increased CYP4A14 expression and 20-HETE ***4A14 could represent a useful target for the treatment of Ang Ⅱ-elici

关键词： CYP4A14 renal fibrosis angiotensin Ⅱ

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Induction of Cytochrome P450 4A14 is associated with Angiote...

绿色技术创新体系的历史进程、演进逻辑与动力机制——基于历史制度主义的分析

脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者： Yunfeng Zhou Min Gao Shizheng Huang shiqi ye Youfei Guan Department of Physiology ShenzhenUniversity Health Science Center

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行政论坛 2023年第6期30卷 91-99页

作者：张伟叶诗奇四川大学公共管理学院四川成都610065

绿色技术创新与生态文明建设密不可分,科学认知其历史进程、演进逻辑和动力机制是推动中国式现代化与全球环境治理的重要议题。基于历史制度主义视角,可以将我国绿色技术创新体系演变划分为艰难起步、快速发展和深度融合等三个历史阶段... 详细信息

绿色技术创新与生态文明建设密不可分,科学认知其历史进程、演进逻辑和动力机制是推动中国式现代化与全球环境治理的重要议题。基于历史制度主义视角,可以将我国绿色技术创新体系演变划分为艰难起步、快速发展和深度融合等三个历史阶段。制度的稳定政策场域、自适应机制和思维定式使绿色技术创新体系的渐进发展产生了强烈的路径依赖。环境保护基本国策地位的确立、绿色技术激励体系的构建、绿色技术体制改革的深化、巴黎气候变化大会的召开以及“碳达峰”“碳中和”目标的提出等,是绿色技术创新体系变迁的关键节点。在动力机制方面,政治、经济和社会构成制度变迁的基础动力;学习效应、协同效应和适应性预期构成制度变迁的内生动力;微观行动主体间的利益博弈构成制度变迁的直接动力。未来,绿色技术创新体系将通过加深市场化、增强国际化、深化智能化,赋能生态文明建设和经济高质量发展。

关键词：绿色发展绿色技术绿色技术创新体系历史制度主义

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基于布尔矩阵的高价值度关联规则挖掘算法

科技管理研究 2014年第6期34卷 188-191页

作者：叶世绮孙振赵喆暨南大学

传统的挖掘算法Apriori是依据统计学中的数据显著性挖掘关联规则,需多次扫描数据库,效率较低,且忽视了数据显著性与价值性不匹配的问题。针对"大数据"下容易产生数量繁多但无效的关联规则,通过采用基于布尔矩阵挖掘关联规则的算法,只扫... 详细信息

传统的挖掘算法Apriori是依据统计学中的数据显著性挖掘关联规则,需多次扫描数据库,效率较低,且忽视了数据显著性与价值性不匹配的问题。针对"大数据"下容易产生数量繁多但无效的关联规则,通过采用基于布尔矩阵挖掘关联规则的算法,只扫描一次数据库,得出布尔矩阵及相应的利润矩阵,随后根据"二八法则"设定对客户最具吸引力的"最小价值度",最终挖掘出高价值的关联规则,从而提高规则挖掘的效率及价值。

关键词：关联规则布尔矩阵规则相关项布尔矩阵平均利润矩阵最小价值度

Overexpression of low-density lipoprotein receptor prevents neurotoxic polarization of astrocytes via inhibiting NLRP3 inflammasome activation in experimental ischemic stroke

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Neural Regeneration Research 2025年第2期20卷 491-502页

作者： Shuai Feng Juanji Li Tingting Liu shiqi Huang Xiangliang Chen Shen Liu Junshan Zhou Hongdong Zhao ye Hong Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Nanjing Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjingJiangsu ProvinceChina

Neurotoxic astrocytes are a promising therapeutic target for the attenuation of cerebral ischemia/reperfusion ***-density lipoprotein receptor,a classic cholesterol regulatory receptor,has been found to inhibit NLR family pyrin domain containing protein 3(NLRP3)inflammasome activation in neurons following ischemic stroke and to suppress the activation of microglia and astrocytes in individuals with Alzheimer’s ***,little is known about the effects of low-density lipoprotein receptor on astrocytic activation in ischemic *** address this issue in the present study,we examined the mechanisms by which low-density lipoprotein receptor regulates astrocytic polarization in ischemic stroke ***,we examined low-density lipoprotein receptor expression in astrocytes via immunofluorescence staining and western blotting *** observed significant downregulation of low-density lipoprotein receptor following middle cerebral artery occlusion reperfusion and oxygen-glucose deprivation/***,we induced the astrocyte-specific overexpression of low-density lipoprotein receptor using astrocyte-specific adeno-associated ***-density lipoprotein receptor overexpression in astrocytes improved neurological outcomes in middle cerebral artery occlusion mice and reversed neurotoxic astrocytes to create a neuroprotective ***,we found that the overexpression of low-density lipoprotein receptor inhibited NLRP3 inflammasome activation in oxygen-glucose deprivation/reoxygenation injured astrocytes and that the addition of nigericin,an NLRP3 agonist,restored the neurotoxic astrocyte *** findings suggest that low-density lipoprotein receptor could inhibit the NLRP3-meidiated neurotoxic polarization of astrocytes and that increasing low-density lipoprotein receptor in astrocytes might represent a novel strategy for treating cerebral ischemic stroke.

关键词： inflammation ischemia/reperfusion injury ischemic stroke low-density lipoprotein receptor neuroprotective astrocytes neurotoxic astrocytes NLRP3 inflammasome polarization