检索结果-南通市图书馆

Multiplexed bioluminescence imaging of cancer cell response to hypoxia and inflammation in the caudal-artery injection model of bone metastasis during zoledronic acid treatment

引用

Journal of Cancer Metastasis and Treatment 2021年第1期7卷 69-80页

作者： Misa Minegishi Takahiro Kuchimaru Kenji Nakagawa Tatsuhiro Isozaki Satoshi Fujimori Tetsuya Kadonosono shinae kizaka-kondoh School of Life Science and Technology Tokyo Institute of TechnologyKanagawa 226-8501Japan Center for Molecular Medicine Jichi Medical UniversityTochigi 329-0498Japan.

Aim:Therapeutic agents suppressing bone remodeling have been clinically approved to delay metastatic progression and skeletal-related events in patients with bone metastasis.However,therapeutic agents including zoledronic acid(ZA)are insufficient to regress established bone metastasis.Therefore,new treatment strategies are desired,and unraveling the status of cancer cells during bone metastatic progression will help develop therapeutic strategies.Methods:We developed a unique multiplexed reporter system for bioluminescent imaging(MRS-BLI)using three luciferase reporter genes.This system allows for the noninvasive and quantitative monitoring of tumor growth and activities of nuclear factor-kappa B(NF-κB)and hypoxia-inducible factor(HIF),which are the key transcriptional factors in response to inflammation and hypoxia,respectively.PC-3/MRS-BLI,a human prostate cancer cell line that stably retains the MRS-BLI reporter genes,was applied to the caudal-artery injection model of bone metastasis to observe the status of cancer cells during bone metastasis development and ZA treatment(<1 month).Results:MRS-BLI reveals key events during the bone metastasis development:NF-κB and HIF are activated in cancer cells after migration to the bone marrow and are transiently reduced,followed by rapid activation before proliferation begins.ZA treatment suppresses the growth of metastasized cancer cells by suppressing NF-κB and HIF activities that may be indirectly induced by osteoclast activation.Conclusion:By visualizing the NF-κB and HIF activities of PC-3/MRS-BLI in bone,MRS-BLI has enabled new discoveries regarding the regulation of bone metastases.Further analysis of the progression of bone metastases using MRS-BLI may provide important information for developing new therapeutic strategies.

关键词： Bone metastasis multiplexed bioluminescence imaging zoledronic acid nuclear factor-kappa B hypoxia inducible factor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

ROR2 regulates the survival of murine osteosarcoma cells in lung capillaries

引用

Journal of Cancer Metastasis and Treatment 2020年第1期6卷 611-621页

作者： Diem Thi Phuong Tran Takahiro Kuchimaru Mongkol Pongsuchart Kha The Nguyen John Clyde Co Soriano Tetsuya Kadonosono shinae kizaka-kondoh School of Life Science and Technology Tokyo Institute of TechnologyYokohama 226-8501Japan Center for Molecular Medicine Jichi Medical UniversityTochigi 329-0498Japan

Aim:Lung metastasis is a leading cause of death in patients with osteosarcoma(OS).No effective therapy exists that improves the five-year overall survival rate of OS patients with metastasis.Therefore,finding novel therapeutic targets will help develop new treatment strategies for OS patients with lung metastasis.Methods:Based on analysis of gene expression profiles between sublines of the Dunn OS LM8 cell line with high(LM8-H)and low(LM8-L)metastatic ability,we have identified Wnt signal-related genes that play an important role in lung metastasis of OS.Function of the genes was investigated by establishing sublines of gene knockout and assessing their metastatic ability using a mouse lung metastasis model.The molecular mechanism underlying the function of the genes was further investigated by in vitro experiments.Results:We have identified that receptor tyrosine kinase-like orphan receptor 2(ROR2),a receptor of the non-canonical Wnt signaling pathway,was involved in OS cell survival in lung capillaries during metastasis.LM8-H knocked out of Ror2(H/Ror2-KO)significantly reduced lung metastasis by decreasing the viability in lung capillaries 48 h after intravenous injection.In vitro study revealed that ROR2 increased anoikis resistance through AKT activation.Reconstitution of ROR2 expression in H/Ror2-KO cells restored their metastatic ability and viability in lung capillaries.Conclusion:The results demonstrate a novel ROR2 function in OS lung metastasis and may inform new treatment strategies for OS patients.

关键词： Osteosarcoma lung metastasis cell survival lung capillary ROR2

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lithospheric thinning and ignition of a Cordilleran magmatic flare-up:Geochemical and O-Hf isotopic constraints from Cretaceous plutons in southern Korea

引用

Geoscience Frontiers 2023年第1期14卷 327-345页

作者： Albert Chang-sik Cheong Min-Ji Jung Hui JeJo Jeongmin Kim shinae Lee Youn-Joong Jeong Korea Basic Science Institute Ochang Center Cheongju 28119Republic of Korea Graduate School of Analytical Science and Technology Chungnam National UniversityDaejeon 34134Republic of Korea Korea Institute of Geoscience and Mineral Resources Daejeon 34132Republic of Korea

Northeast Asian continental margins contain the products of magma emplacement driven by prolonged subduction of the(paleo-)Pacific plate.As observed in many Cordilleran arcs,magmatic evolution in this area was punctuated by high-volume pulses amid background periods.The present study investigates the early evolution of the Cretaceous magmatic flare-up using new and published geochronological,geochemical,and O-Hf isotope data from plutonic rocks in the southern Korean Peninsula.After a long(~50 m.y.)magmatic hiatus and the development of the Honam Shear Zone through flat-slab subduction,the Cretaceous flare-up began with the intrusion of monzonites,granodiorites,and granites in the inboard Gyeonggi Massif and the intervening Okcheon Belt.Compared to Jurassic granitoids formed during the former flare-up,Albian(~111 Ma)monzonites found in the Eopyeong area of the Okcheon Belt have distinctly higher zirconε_(Hf)(t)(-7.5±1.3)andδ^(18)O(7.78‰±0.25‰)values and lower wholerock La/Yb and Sr/Y ratios.The voluminous coeval granodiorite and granite plutons in the Gyeonggi Massif are further reduced in Sr/Y and to a lesser extent,in La/Yb,and have higher zirconε_(Hf)(t)values(-13 to-19)than the Precambrian basement(ca.-30).These chemical and isotopic features indicate that Early Cretaceous lithospheric thinning,most likely resulting from delamination of tectonically and magmatically overthickened lithospheric keel that was metasomatized during prior subduction episodes,and consequent asthenospheric upwelling played vital roles in igniting the magmatic flare-up.The O-Hf isotopic ranges of synmagmatic zircons from the Albian plutons and their Paleoproterozoic and Jurassic inheritance attest to the involvement of lithospheric mantle and crustal basement in magma generation during this decratonization event.Arc magmatism then migrated trenchward and culminated in the Late Cretaceous,yielding widespread granitoid rocks emplaced at shallow crustal levels.The early Late Cretaceous(94-85 Ma)granites now prevalent in Seoraksan-Woraksa

关键词： Cordilleran arc Cretaceous flare-up Korean Peninsula Lithospheric thinning Crustal rejuvenation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Crystal structure of Arabidopsis thaliana RabA1a

引用

Journal of Integrative Plant Biology 2019年第2期61卷 93-109页

作者： Ji-Sook Yun Sung Chul Ha shinae Kim Yeon-Gil Kim Hyeran Kim Jeong Ho Chang Department of Biology Education Kyungpook National University Beamline Science Division Pohang Accelerator Laboratory 3. Department of Biological Sciences Kangwon National University

RabGTPase is a member of the Ras superfamily of small GTPases, which share a GTP-binding pocket containing highly conserved motifs that promote GTP hydrolysis. In Arabidopsis, the RabA group, which corresponds to the Rabgroup in animals, functions in the recycling of endosomes that control docking and fusion during vesicle transport. However, their molecular mechanisms remain unknown. In this study, we determined the crystal structures of the GDP-bound inactive form and both GppNHp-and GTP-bound active forms of RabA1a, at resolutions of 2.8, 2.6, and 2.6 A?,respectively. A bound sulfate ion in the active site of the GDP-bound structure stabilized Switch Ⅱ by bridging the interaction between a magnesium ion and Arg74.Comparisons of the two states of RabA1a with Rabproteins revealed clear differences in the Switch Ⅰ and Ⅱ loops. These results suggested that conformational change of the Switch regions of RabA1a, derived by GTP or GDP binding, could maintain subcellular membrane traffic through the specific interaction of effector molecules.

关键词： GDP Crystal structure of Arabidopsis thaliana RabA Figure

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Zircon U–Pb geochronology and Hf isotope geochemistry of magmatic and metamorphic rocks from the Hida Belt, southwest Japan

引用

Geoscience Frontiers 2021年第4期12卷 185-201页

作者： Deung-Lyong Cho Tae-Ho Lee Yutaka Takahashi Takenori Kato Keewook Yi shinae Lee Albert Chang-sik Cheong Korea Institute of Geoscience and Mineral Resources Daejeon 34132Republic of Korea Geological Survey of Japan Tsukuba 305-8567Japan Institute for Space-Earth Environmental Research Nagoya UniversityNagoya 464-8601Japan Korea Basic Science Institute Ochang Center Cheongju 28119Republic of Korea

Zircon U–Pb and Hf isotope data integrated in this study for magmatic and metamorphic rocks fromthe Hida Belt,southwest Japan,lead to a new understanding of the evolution of the Cordilleran arc system along the ancestral margins of present-day Northeast Asia.Ion microprobe data for magmatic zircon domains from eight mafic tointermediate orthogneisses in the Tateyama and Tsunogawa areas yielded weighted mean 206Pb/238U ages spanning the entire Permian period(302–254 Ma).Under cathodoluminescence,primary magmatic growth zones in the zircon crystals were observed to be partially or completely replaced by inward-penetrating,irregularly curved featureless or weakly zoned secondary domains that mostly yielded U–Pb ages of 250–240 Ma and relatively high Th/U ratios(>0.2).These secondary domains are considered to have been formed by solid-state recrystallization during thermal overprints associated with intrusions of Hida granitoids.Available whole-rock geochemical and Sr–Nd isotope data as well as zircon age spectra corroborate that the Hida Belt comprises the Paleozoic–Mesozoic Cordilleran arc system built upon the margin of the North China Craton,together with the YeongnamMassif in southern Korea.The arcmagmatismalong this systemwas commenced in the Carboniferous and culminated in the Permian–Triassic transition period.Highly positiveεHf(t)values(>+12)of late Carboniferous to early Permian detrital zircons in the Hida paragneisses indicate that there was significant input from the depleted asthenospheric mantle and/or its crustal derivatives in the early stage of arc magmatism.On the other hand,near-chondriticεHf(t)values(+5 to−2)of magmatic zircons from late Permian Hida orthogneisses suggest a lithospheric mantle origin.Hf isotopic differences between magmatic zircon cores and the secondary rims observed in some orthogneiss samples clearly indicate that the zircons were chemically open to fluids or melts during thermal overprints.Resumed highly positive zirconεHf(t)values(>+9)shared by Early Jurassic granitoids in t

关键词： Hida Belt Zircon U–Pb age Hf isotope Cordilleran arc Yeongnam Massif

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

结构损伤位置识别的位移DLV方法

引用

工程力学 2009年第5期26卷 209-215页

作者：张宇鑫 shinae Jang B.F.Spencer 上海师范大学建筑工程学院上海201418 伊利诺伊大学香槟分校土木工程系美国61801

DLV(damage locating vector)家族方法由BERNAL于2002年提出后,因其创新巧妙的求解思想而引起结构健康监测研究领域的广泛关注。其应用于加速度测试数据进行结构损伤定位识别的有效性已通过多组试验得到证明。然而基于静力测试数据进行... 详细信息

DLV(damage locating vector)家族方法由BERNAL于2002年提出后,因其创新巧妙的求解思想而引起结构健康监测研究领域的广泛关注。其应用于加速度测试数据进行结构损伤定位识别的有效性已通过多组试验得到证明。然而基于静力测试数据进行结构损伤识别将具有更高的精度和数值稳定性。该文基于静力位移测试数据,构建结构柔度矩阵,提出位移DLV方法,并编制了通用有限元程序。通过数值仿真分析对位移DLV方法的有效性进行了研究,数值分析结果表明:位移DLV方法能够利用少数测点信息识别出结构的损伤位置。同时研究了若干影响因素如测点数量、损伤位置、测点布置、测量误差等对方法应用的影响。

关键词：结构健康监测损伤识别 DLV(damage locating vector) 静力位移测点布置

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Crystal structure of Arabidopsis thaliana RabA_(1a)

引用

Journal of Integrative Plant Biology 2019年第2期61卷 93-109页

作者： Ji-Sook Yun Sung Chul Ha shinae Kim Yeon-Gil Kim Hyeran Kim Jeong Ho Chang Department of Biology Education Kyungpook National University Beamline Science Division Pohang Accelerator Laboratory Department of Biological Sciences Kangwon National University

RabGTPase is a member of the Ras superfamily of small GTPases, which share a GTP-binding pocket containing highly conserved motifs that promote GTP hydrolysis. In Arabidopsis, the RabA group, which corresponds to the Rab_(11) group in animals, functions in the recycling of endosomes that control docking and fusion during vesicle transport. However, their molecular mechanisms remain unknown. In this study, we determined the crystal structures of the GDP-bound inactive form and both GppNHp-and GTP-bound active forms of RabA1a, at resolutions of 2.8, 2.6, and 2.6 A?,respectively. A bound sulfate ion in the active site of the GDP-bound structure stabilized Switch Ⅱ by bridging the interaction between a magnesium ion and Arg74.Comparisons of the two states of RabA1a with Rab_(11) proteins revealed clear differences in the Switch Ⅰ and Ⅱ loops. These results suggested that conformational change of the Switch regions of RabA1a, derived by GTP or GDP binding, could maintain subcellular membrane traffic through the specific interaction of effector molecules.

关键词： GDP Crystal structure of Arabidopsis thaliana RabA Figure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：