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Droplet microfluidic chip for precise monitoring of dynamic solution changes

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纳米技术与精密工程(英文) 2023年第3期6卷 55-63页

作者： Cong Ma Zehang Gao Jianlong Zhao shilun feng School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Department of Clinical Laboratory Third Affiliated Hospital of Guangzhou Medical UniversityGuangdong 510150China

In this work,an automated microfluidic chip that uses negative pressure to sample and analyze solutions with high temporal resolution was *** chip has a T-shaped channel for mixing the sample with a fluorescent indicator,a flow-focusing channel for generating droplets in oil,and a long storage channel for incubating and detecting the *** monitoring the fluorescence intensity of the droplets,the device could detect changes in solution accurately over *** chip can generate droplets at frequencies of up to 42 Hz with a mixing ratio of 1:1 and a temporal resolution of 3–6 *** had excellent linearity in detecting fluorescein solution in the concentration range 1–5μ*** droplet microfluidic chip provides several advantages over traditional methods,including high temporal resolution,stable droplet generation,and faster flow *** approach could be applied to monitoring calcium ions with a dynamic range from 102 to 107 nM and a detection limit of 10 nM.

关键词： Microfluidic chip Droplet sampling Fluorescence detection Calcium ion dynamics Temporal resolution

A 3D-printed modular magnetic digital microfluidic architecture for on-demand bioanalysis

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Microsystems & Nanoengineering 2020年第1期6卷 785-795页

作者： Pojchanun Kanitthamniyom Aiwu Zhou shilun feng Aiqun Liu Shawn Vasoo Yi Zhang Singapore Centre for 3D Printing School of Mechanical and Aerospace EngineeringNanyang Technological UniversitySingaporeSingapore School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingaporeSingapore School of Electrical and Electronic Engineering Nanyang Technological UniversitySingaporeSingapore National Center for Infectious Disease Tan Tock Seng HospitalSingaporeSingapore

Magnetic digital microfluidics(MDM)manipulates fluids in the form of droplets on an open substrate,and incorporates surface energy traps(SETs)to facilitate the droplet *** MDM devices are fabricated monolithically,which makes it difficult to modify the device configuration without completely overhauling the original *** this paper,we present a modular MDM architecture that enables rapid on-demand configuration and re-configuration of MDM platforms for customized *** modular component contains a SET and a Lego-like antistud that fits onto a base board with Lego-like *** illustrate the versatility of the modular MDM architecture in biomarker sensing,pathogen identification,antibiotic resistance determination,and biochemical quantification by demonstrating immunoassays,phenotypical assays and enzymatic assays on various modular MDM platforms configured on demand to accomplish the fluidic operations required by assorted bioanalytical *** modular MDM architecture promises great potential for point-of-care diagnostics by offering on-demand customization of testing platforms for various categories of diagnostic *** also provides a new avenue for microfluidic assay development with its high configurability which would significantly reduce the time and cost of the development cycle.

关键词： configuration operations enable

Microfluidics embedded with microelectrodes for electrostimulation of neural stem cells proliferation

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Chinese Chemical Letters 2022年第3期33卷 1308-1312页

作者： Qian Li Bodong Kang Libin Wang Tao Chen Yu Zhao shilun feng Rongjing Li Hongtian Zhang Institute of Advanced Photonics Technology Faculty of Materials and ManufacturingBeijing University of TechnologyBeijing 100124China Key Laboratory of Trans-scale Laser Manufacturing Technology(Beijing University of Technology) Ministry of EducationBeijing 100124China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Department of Neurosurgery The 7th Medical CenterGeneral Hospital of the Chinese People's Liberation ArmyBeijing 100700China

The regeneration of the injured nerve and recovery of its function have brought attention in the medical field. Electrical stimulation(ES) can enhance the cellular biological behavior and has been widely studied in the treatment of neurological diseases. Microfluidic technology can provide a cell culture platform with the well-controlled environment. Here a novel microfluidic/microelectrode composite microdevice was developed by embedding the microelectrodes to the microfluidic platform, in which microfluidics provided a controlled cell culture platform, and ES promoted the NSCs proliferation. We performed ES on rat neural stem cells(NSCs) to observe the effect on their growth, differentiation, proliferation, and preliminary explored the ES influence on cells in vitro. The results of immunofluorescence showed that ES had no significant effect on the NSCs specific expression, and the NSCs specific expression reached 98.9%± 0.4% after three days of ES. In addition, ES significantly promoted cell growth and the cell proliferation rate reached 49.41%. To conclude, the microfluidic/microelectrode composite microdevice can play a positive role in the nerve injury repair and fundamental research of neurological diseases.

关键词： Microfluidics Microelectrode Electrical stimulation NSCs Proliferation

Demands and technical developments of clinical flow cytometry with emphasis in quantitative,spectral,and imaging capabilities

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Nanotechnology and Precision Engineering 2022年第4期5卷 46-55页

作者： Ting Zhang Mengge Gao Xiao Chen Chiyuan Gao shilun feng Deyong Chen Junbo Wang Xiaosu Zhao Jian Chen State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190People’s Republic of China School of Future Technology University of Chinese Academy of SciencesBeijing 100049People’s Republic of China Peking University People’s Hospital Peking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044People’s Republic of China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050People’s Republic of China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049People’s Republic of China

As the gold-standard method for single-cell analysis,flow cytometry enables high-throughput and multiple-parameter characterization of individual biological *** review highlights the demands for clinical flow cytometry in laboratory hematology(e.g.,diagnoses of minimal residual disease and various types of leukemia),summarizes state-of-the-art clinical flow cytometers(e.g.,FACSLyricTMby Becton Dickinson,DxFLEX by Beckman Coulter),then considers innovative technical improvements in flow cytometry(including quantitative,spectral,and imaging approaches)to address the limitations of clinical flow cytometry in hematology ***,driven by these clinical demands,future developments in clinical flow cytometry are suggested.

关键词： Clinical demand Clinical flow cytometry Quantitative flow cytometry Spectral flow cytometry Imaging flow cytometry

Lasing action in microdroplets modulated by interfacial molecular forces

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Advanced Photonics 2021年第1期3卷 82-92页

作者： Zhen Qiao Xuerui Gong Peng Guan Zhiyi Yuan shilun feng Yiyu Zhang Munho Kim Guo-En Chang Yu-Cheng Chen Nanyang Technological University School of Electrical and Electronic Engineering Shanghai Jiao Tong University School of Electronic Information and Electrical Engineering Chinese Academy of Sciences Shanghai Institute of Microsystem and Information TechnologyState Key Laboratory of Transducer Technology Taiwan Chung Cheng University Department of Mechanical Engineering Nanyang Technological University School of Chemical and Biomedical Engineering

Liquid droplets offer a great number of opportunities in biochemical and physical research studies in which droplet-based microlasers have come into play over the past decade. While the recent emergence of droplet lasers has demonstrated their powerful capabilities in amplifying subtle molecular changes inside the cavity, the optical interactions between droplet resonators and an interface remain unclear. We revealed the underlying mechanism of droplet lasers when interacting with a droplet–solid interface and explored its correlation with intermolecular forces. A vertically oriented oscillation mode—arc-like mode—was discovered, where the number of lasing modes and their Q-factors increase with the strength of interfacial hydrophobicity. Both experimental and theoretical results demonstrated that hydrophobicity characterized by contact angle and interfacial tension plays a significant role in the geometry of droplet cavity and laser mode characteristics. Finally, we demonstrated how tiny forces induced by proteins and peptides could strongly modulate the lasing output in droplet resonators. Our findings illustrate the potential of exploiting optical resonators to amplify intermolecular force changes, providing comprehensive insights into lasing actions modulated by interfaces and applications in biophysics.

关键词： microlaser droplet resonator intermolecular forces biointerface lasing action hydrophobicity

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海洋平台桩基的振动台模型试验研究

岩石力学与工程学报 2006年第z1期25卷 3229-3234页

作者：冯士伦王建华天津大学岩土工程研究所天津300072

通过海洋平台桩基的振动台模型试验,获得土层振动过程中桩身弯矩和土层中累积孔压的时间过程曲线。进而提出根据土层中孔压的水平,对静力曲线中的静力进行相应折减,即乘以不大于1的比例系数,作为相应孔压水平时桩基侧向承载特性曲线的... 详细信息

通过海洋平台桩基的振动台模型试验,获得土层振动过程中桩身弯矩和土层中累积孔压的时间过程曲线。进而提出根据土层中孔压的水平,对静力曲线中的静力进行相应折减,即乘以不大于1的比例系数,作为相应孔压水平时桩基侧向承载特性曲线的表征方法。利用该表征方法把试验结果进行了数值模拟,得到能够反映桩基侧向承载特性的比例系数与土层中相应的孔压比之间的关系,即:当土层的孔压比小于0.2时,比例系数为1;当土层的孔压比大于等于0.8时,比例系数为0.1;当土层的孔压比为0.2～0.8时,比例系数随孔压比的增加在1和0.1之间线性减小。

关键词：岩土工程海洋平台孔压桩 p-y曲线振动台试验

On-chip structure-switching aptamer-modified magnetic nanobeads for the continuous monitoring of interferon-gamma ex vivo

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Microsystems & Nanoengineering 2019年第1期5卷 281-291页

作者： Guozhen Liu Chaomin Cao Shengnan Ni shilun feng Hui Wei Graduate School of Biomedical Engineering ARC Centre of Excellence in Nanoscale Biophotonics(CNBP)Faculty of EngineeringThe University of New South WalesSydneyNSW 2052Australia Australian Centre for NanoMedicine UNSW SydneySydneyNSW 2052Australia International Joint Research Center for Intelligent Biosensor Technology and Health College of ChemistryCentral China Normal University430079 WuhanPR China School of Engineering Faculty of Science and EngineeringMacquarie UniversitySydneyNSW 2109Australia

Cytokines are cell signaling molecules that indicate the health status of the *** this study,we developed a microfluidic device integrated with structure-switching aptamers capable of continuously tracking the concentration of the cytokine interferon gamma(IFN-γ)in cell culture medium and blood ***,a ferrocene(Fc)-labeled structure-switching signaling aptamer with a hairpin structure targeting IFN-γwas immobilized on magnetic nanobeads by the strongest noncovalent interactions between streptavidin and *** aptamer-modified magnetic nanobeads were trapped on a customized microfluidic chip by a magnetic field to form the sensing *** binding of IFN-γcould trigger the hairpin structure of the aptamer to unfold,pushing Fc redox molecules away from the sensing interface and consequently switching off the electrochemical *** change in the redox current of Fc was quantitatively related to the concentration of IFN-γin a linear range of 10–500 pg mL^(−1)and with the lowest detection limit of 6 pgmL^(−1).This microfluidic device was specific to IFN-γin the presence of overabundant serum proteins and allowed the continuous monitoring of IFN-γwithout adding exogenous *** provided a universal point-of-care biosensing platform for the real-time detection of a spectrum of analytes.

关键词： beads structure magnetic

两个六经不能混淆——《伤寒杂病论》的理论来源

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中华中医药杂志 2006年第10期21卷 582-585页

作者：冯世纶卫生部中日友好医院北京100029

很多学者认为张仲景据《黄帝内经》撰成了《伤寒杂病论》(简称为《伤寒》),因而以《黄帝内经》的六经、脏腑经络释《伤寒》,其结果是不能正确理解张仲景医学。笔者据考证资料证实,张仲景不是根据《黄帝内经》撰写的《伤寒》,它的主要内... 详细信息

很多学者认为张仲景据《黄帝内经》撰成了《伤寒杂病论》(简称为《伤寒》),因而以《黄帝内经》的六经、脏腑经络释《伤寒》,其结果是不能正确理解张仲景医学。笔者据考证资料证实,张仲景不是根据《黄帝内经》撰写的《伤寒》,它的主要内容方证,来自于《汤液经法》等经方医学。《伤寒》主要继承八纲辨证。并加入半表半里理念而形成了六经辨证。这样以六经为纲,以方证为目,形成了独特的辨证论治体系。《伤寒》的六经与《黄帝内经》的六经是不同的理论概念,在解读《伤寒》时,两种概念不能混淆。

关键词：伤寒论半表半里六经经络脏腑

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XJ自升式平台拖航稳性计算方法

船舶工程 2017年第12期39卷 65-68,74页

作者：向嘉隽毛建斌冯士伦冯旭天津大学建筑工程学院天津300072 海洋石油工程股份有限公司天津300451

针对XJ自升式平台,采用Moses软件建立平台模型,分别计算该平台在油田拖航和远洋拖航时的完整稳性以及破舱稳性。将实际拖航时的稳性情况与数值模拟结果进行对比发现,用Moses简化建模来模拟计算平台拖航稳性所产生的模型误差对最终稳性... 详细信息

针对XJ自升式平台,采用Moses软件建立平台模型,分别计算该平台在油田拖航和远洋拖航时的完整稳性以及破舱稳性。将实际拖航时的稳性情况与数值模拟结果进行对比发现,用Moses简化建模来模拟计算平台拖航稳性所产生的模型误差对最终稳性计算结果的影响较小。

关键词：自升式平台 Moses 完整稳性破舱稳性