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Advances in Bioscience and Biotechnology 2011年第2期2卷 97-102页

作者： Teruaki Kobayash Tatsuro shibui 不详

An mRNA display system using synthetic DNA cod-ing for random 10- and 20-amino-acid peptide li-braries was employed to isolate mimotopes that could substitute for the anti-human VEGF antibody Bevacizumab. After six rounds of affinity selection, three clones that bound to the antibody were isolated. Their random-peptide portions were chemically syn-thesized, and further characterized. All of the pep-tides showed clear specific binding to the antibody. Two of them were further conjugated with Keyhole limpet hemocyanin (KLH) to immunize a rabbit. Af-ter five immunizations biweekly, antibodies to the peptides were purified with a column conjugated with the peptides. The purified antibodies reacted specifically to the antibody’s original antigen, human VEGF. mRNA displays could be useful for the isola-tion of mimotopes for vaccines to substitute for ther-apeutic antibodies.

关键词： Random Peptide mRNA Display Mimotope Antigen Antibody Vaccine

Stability of Document Analysis and Recognition for University Evaluation Reports

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Stability of Document Analysis and Recognition for Universit...

High-level secretory expression, purification, and characterization of an anti-human Her II monoclonal antibody, trastuzumab, in the methylotrophic yeast <i>Pichia pastoris</i>

2012 International Conference on Education and Management Innovation（ICEMI 2012）

作者： Masaaki Ida Susumu shibui National Institution for Academic Degrees and University Evaluation

Multivariate analysis and natural language processing methods for textual information have become popular research techniques which can deepen understanding on of accumulated text data, such as evaluation reports, and also have possibility to lead new knowledge discovery. In this paper we focus on contents of textual information in evaluation reports of National University Corporation Evaluation in Japan. We conducted research on information extracting and text analyzing for grasping global and local features of the evaluation reports. Moreover, we considered stability of the analysis and recognition in case of textual data fluctuation.

关键词： Evaluation reports document analysis recognition stability.

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Advances in Bioscience and Biotechnology 2013年第5期4卷 640-646页

作者： Tatsuro shibui Keisuke Bando Satoru Misawa API Corporation Bio and Chemical Process Discovery Laboratory 1000 Kamoshida-Cho Aoba-Ku Yokohama Japan Food Biotechnology Laboratory School of Food Sciences Nippon Veterinary and Life Science University 2-17-5 Sakai Musashi- no-Shi Tokyo Japan Mitsubishi Chemical Group Science and Technology Research Center 1000 Kamoshida-Cho Aoba-Ku Yokohama Japan

DNA fragments encoding the light chain and heavy chain genes of an anti-human HER II antibody, trastuzumab, fused with an egg-lysozyme signal peptide were synthesized based on the codon bias of the methylotrophic yeast Pichia pastoris. These fragments were inserted into a site between the AOX 1-promoter and -terminator in pPICZ A to be expressed by P. pastoris. The expression vector was linearized, and introduced into P. pastoris GS115 by electroporation. After the checking of several transformants with PCR to ensure a precise insertion, one was selected and cultured to examine antibody production. The level of production reached 10 mg/L in a flask with medium containing 1% methanol. The heavy chain and light chain of the product were assembled to form a hetero tetramer, as detected by dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). N-terminal amino acid sequencing revealed that the signal peptides of both chains were well processed. The mobility of the product in SDS-PAGE after treatment with Peptide N-Glycosidase F indicated the heavy chain to be N-glycosylated. Further analysis of the N-glycans with a mass spectrometer revealed a mixture of Man9-GlcNAc2, Man10-GlcNAc2, Man11-GlcNAc2 and Man12-GlcNAc2, but no hyper-mannosylated glycans. ELISA, surface plasmon resonance, and flow cytometric studies showed the affinity curve and Kd value for the antigen, HER II, and reactivity to a HER2-overexpressing breast cancer cell-line, SK-BR-3, to be almost the same as for the clinically used trastuzumab produced by CHO.

关键词： Pichia Pastoris Antibody Secretory Production Glycocylation Her II

A New Tandem Gene Construction Method Involving a Cloning System Using <i>Poxvirus DNA polymerase</i>, and Its Application to Gene Expression

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Advances in Bioscience and Biotechnology 2014年第10期5卷 838-845页

作者： Tatsuro shibui Daisuke Sakaguchi Hiroyoshi Hara Food Biotechnology Laboratory School of Food Sciences Nippon Veterinary and Life Science University Tokyo Japan

A simple method for constructing polymerized genes using only restriction enzymes and commercially available cloning systems was established. In this system, gel isolations or purifications of target genes after restriction enzyme digestions or PCR amplifications, which often cause errors and mutations in the target gene sequence, are not necessary. To verify the usefulness of this method, one, two, four, eight, and sixteen tandem-repeats of the Green Fluorescent Protein (GFP) expression gene in Escherichia coli were sequentially constructed. Efficacies of the GFP gene expression of those plasmids in E. coli showed an increasing trend in accordance with the copy numbers of the gene. On SDS polyacrylamide gel electrophoresis with Coomassie blue staining, no expressed protein could be seen in E. coli cells harboring plasmids that contained one or two copies of the gene. However, expressed protein bands in E. coli cells were clearly detected with 4 copies of the gene. In quantitative analyses involving green fluorescence intensities per culture volume, the expression level in E. coli with 16 copies of the gene was 36.3-fold higher than that in E. coli with one copy at 22 hours after induction.

关键词： Poxvirus DNA polymerase Gene Cloning Tandem Repeat GFP T7 RNA Polymerase

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仲夏夜之梦

现代装饰（家居） 2015年第7期 122-129页

作者： Lisa Corti Magenta Studio vij5 DEDON Anastasiya Koshcheeva 张予馨 shibui 范嘉苑

在莎士比亚的《仲夏夜之梦》里,淘气的迫克小精灵将魔汁滴在了熟睡的两位年轻人和仙后的眼皮上,由此展开了一段让人啼笑皆非的＂锴爱＂故事。戏剧的结尾,魔力解除,有情人终成眷属,仙人们也和好如初。一切都如同一场奇幻的梦,开始和终结... 详细信息

在莎士比亚的《仲夏夜之梦》里,淘气的迫克小精灵将魔汁滴在了熟睡的两位年轻人和仙后的眼皮上,由此展开了一段让人啼笑皆非的＂锴爱＂故事。戏剧的结尾,魔力解除,有情人终成眷属,仙人们也和好如初。一切都如同一场奇幻的梦,开始和终结于夏夜,消散在微热的空气之中。夏天的夜晚,总是带着些许梦幻。它像是一幅立体的油画,背景是透净的深蓝色,泛着银光的繁星点缀在画布的上头,月光悄悄穿过树梢,斑驳了大地;零星的萤火虫点亮了画面,赋予了夜色轻盈的动态;树上的蝉鸣打破了夏夜的静谧,仿佛在集体演唱着夏日的赞歌……

关键词：仲夏夜之梦仙后终成眷属深蓝色梦幻色彩脚凳设计原型给你家具设计金属框架

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家饰物语

现代装饰（家居） 2015年第1期 50-55页

作者： Alexandre Dubreuil Studio Bandit Machot Dekorando Team DesignByThem Daff Kirsty Whyte KaschKasch Kenyon Yeh molodesign Nasonmoretti Ontwerpduo Ramirezi Carrillo Sigurjon Palsson Stephanie Boira shibui Wallace Sewell Tumbar Zak 伏平范嘉苑

事实证明,随着时间的推移,居室装修只能贬值、落伍与淘汰。在物质生活高度发展,精神生活愈加讲究的今天,“轻装修、重装饰”的理念已经越来越多地被大多数的家庭所接受。人们扭转了以往传统的观念,不再追求过于复杂的装修,而是更注重后... 详细信息

事实证明,随着时间的推移,居室装修只能贬值、落伍与淘汰。在物质生活高度发展,精神生活愈加讲究的今天,“轻装修、重装饰”的理念已经越来越多地被大多数的家庭所接受。人们扭转了以往传统的观念,不再追求过于复杂的装修,而是更注重后期的软性装饰,追求生活品位和崇尚个性,

关键词：饰物居室装修物质生活精神生活生活品位装饰

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几何之美

现代装饰（家居） 2015年第2期 132-139页

作者： Meike Harde shibui Florian Schmid Sebastian Errazuriz muuto Maurizio Bernabei Line Studio Studio North FOAM&Perla Valtierra Liliana Ovalle Giopato&Coombes Staffan Holm Daniel Becker Design Studio Bloomingville 范嘉苑

牛顿说:"几何学的简洁美却又正是几何学之所以完美的核心所在。"在数学的世界里,几何学作为一个重要分支,它以其独特的图形美感著称,从点、线、面到平面图形再到立体图形,这些丰富的元素是那么平凡,却又是那么奇妙,它们共同组合在一起,... 详细信息

牛顿说:"几何学的简洁美却又正是几何学之所以完美的核心所在。"在数学的世界里,几何学作为一个重要分支,它以其独特的图形美感著称,从点、线、面到平面图形再到立体图形,这些丰富的元素是那么平凡,却又是那么奇妙,它们共同组合在一起,总能变幻出层出不穷的美丽。可以说,这个大干世界的每一样东西即便再复杂,其结构都离不开最基本的几何构成,把它们还原成最初的形态,也许会是简单的圆形、三角形、正方形、多边形……这份最原始的几何美也正是万物的魅力所在。

关键词：立体图形平面图形蒙德里安组装方式 liana 系列作品六角形设计主题

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卵巢癌患者个人掌控感现状及影响因素分析

天津护理 2023年第6期31卷 637-641页

作者：吕世慧王田田周超刘欢张生霞董雷雷张振华青岛大学附属医院山东青岛266035

目的:了解卵巢癌患者个人掌控感现状并分析其影响因素。方法:选取2022年2月至9月青岛市某三级甲等综合医院妇科收治的196例卵巢癌患者为研究对象,采用一般资料调查表、个人掌控感量表(PMS)、疾病进展恐惧简化量表(FoP-Q-SF)、一般自我... 详细信息

目的:了解卵巢癌患者个人掌控感现状并分析其影响因素。方法:选取2022年2月至9月青岛市某三级甲等综合医院妇科收治的196例卵巢癌患者为研究对象,采用一般资料调查表、个人掌控感量表(PMS)、疾病进展恐惧简化量表(FoP-Q-SF)、一般自我效能量表(GSES)、社会支持评定量表(SSRS)进行问卷调查。结果:卵巢癌患者个人掌控感得分为(25.50±5.14)分。多重线性回归分析结果显示,病程、疾病进展复发恐惧、自我效能、社会支持是卵巢癌患者个人掌控感的影响因素(P<0.05)。结论:卵巢癌患者个人掌控感水平有待进一步提高,医务人员应重视病程短、疾病进展复发恐惧心理严重、自我效能及社会支持水平较低的患者,有针对性实施临床护理干预措施,以提升卵巢癌患者个人掌控感水平。

关键词：卵巢癌个人掌控感自我效能社会支持疾病进展复发恐惧

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预制装配式混凝土综合管廊施工工艺

建筑施工 2020年第5期42卷 796-800页

作者：何昌杰陈绪林彭亿洲刘世辉杨鑫蕊温利军陈昊杨才千中建五局第三建设有限公司湖南长沙410004 湘潭大学土木工程与力学学院湖南湘潭411105

通过查阅大量与预制装配式混凝土综合管廊相关的文献资料,简述了国内外预制装配式混凝土综合管廊的发展历程,梳理了如今国内外的学术研究现状。通过对当前预制装配式综合管廊研究中管廊的结构形式、防水性能、抗震性能及抗疲劳性能等热... 详细信息

通过查阅大量与预制装配式混凝土综合管廊相关的文献资料,简述了国内外预制装配式混凝土综合管廊的发展历程,梳理了如今国内外的学术研究现状。通过对当前预制装配式综合管廊研究中管廊的结构形式、防水性能、抗震性能及抗疲劳性能等热点问题的分析,找出了综合管廊建设中预制装配式技术的研究和应用的方向,以期为预制装配式混凝土综合管廊的学术研究提供新的视角和基础资料。

关键词：综合管廊预制装配式结构结构形式防水抗震抗疲劳性