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F-Actin reassembly during focal adhesion impacts single cell mechanics and nanoscale membrane structure

Science China Chemistry 2012年第9期55卷 1922-1930页

作者： ZIMMER Christopher C SHI LiFang shih yiping LI JieRen JIN LeeWay LO SuHao LIU GangYu Department of Chemistry University of California Davis CA 95616 USA Department of Biochemistry and Molecular Medicine Center.for Tissue Regeneration and Repair University of California Davis Medical Center Sacramento CA 95817 USA M.I.N.D. Institute and Department of Pathology and Laboratory Medicine ' Alzheimer's Disease Center University of California Davis Medical Center Sacramento CA 95817 USA

Focal adhesions play an important role in cell spreading,migration,and overall mechanical *** relationship of cell structural and mechanical properties was investigated in the context of focal adhesion *** atomic force microscopy(AFM) and laser scanning confocal microscopy(LSCM) was utilized to measure single cell mechanics,in correlation with cellular morphology and membrane structures at a nanometer *** stages of focal adhesion were verified via confocal fluorescent studies,which confirmed three representative F-actin assemblies,actin dot,filaments network,and long and aligned fibrous bundles at ***-deformation profiles of living cells were measured at the single cell level,and displayed as a function of height deformation,relative height deformation and relative volume *** focal adhesion progresses,single cell compression profiles indicate that both membrane and cytoskeleton stiffen,while spreading increases especially from focal complex to focal ***,AFM imaging reveals morphological geometries of spherical cap,spreading with polygon boundaries,and elongated or polarized *** features are dominated by protrusions of 41-207 nm tall,short rods with 1-6 μm in length and 10.2-80.0 nm in height,and long fibrous features of 31-246 nm tall,*** protrusion is attributed to local membrane folding,and the rod and fibrous features are consistent with bilayer decorating over the F-actin *** collectively,the reassembly of F-actin during focal adhesion formation is most likely responsible for the changes in cellular mechanics,spreading morphology,and membrane structural features.

关键词： focal adhesion actin fibers actin assembly fibroblast membrane structure single cell mechanics atomic forcemicroscopy laser scanning confocal microscopy

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