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Molecular hydrogen regulates the expression of miR-9,miR-21 and miR-199 in LPS-activated retinal microglia cells

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 280-285页

作者： Guo-Dan Liu Hong Zhang Lin Wang Qing Han shi-feng zhou Ping Liu Department of Ophthalmology the Fourth Affiliated Hospital of Harbin Medical University Department of Ophthalmology the First Affiliated Hospital of Harbin Medical University

·AIM: To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. ·METHODS: Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen -saturated medium or normal medium without hydrogen. qRT -PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS -induced signaling proteins, including Myd88, IKK -β, NF -kB, and PDCD4, were detected by Western blotting. ·RESULTS: The results demonstrated a marked downregulation of miR -9 and miR -21 and up -regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-kB expression. · CONCLUSION: The results in the present study indicate that miR -9, miR -199 and miR -21 play an important role in the anti -inflammatory regulation of LPS -activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.·

关键词： hydrogen Lipopolysaccharides miR-9 miR-21 miR-199 Toll-like receptor 4

Soluble expression of recomb inant cMyc,Klf4,Oct4,and Sox2 proteins in bacteria and transduction into living cells

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International Journal of Ophthalmology(English edition) 2017年第4期10卷 560-566页

作者： Guo-Dan Liu shi-feng zhou Xu-Chen Ding Chun-Lai Fang Shu-Yong Mi Xiang-Chun Gao Qing Han Department of Ophthalmology the Fourth Affiliated Hospital of Harbin Medical University Department of Emergency the First Affiliated Hospital of Harbin Medical University

AIM：To develop a new method to produce recombinant reprogramming proteins,c Myc,Klf4,Oct4,and Sox2,in soluble format with low cost for the generation of induced pluripotent stem cells（i PSCs）.METHODS：A short polypeptide sequence derived from the HIV trans-activator of transcription protein（TAT） and the nucleus localization signal（NLS） polypeptide were fused to the N terminus of the reprogramming proteins and they were constructed into p Cold-SUMO vector which can extremely improve the solubility of recombinant *** these vector plasmids were transformed into *** BL21（DE3） Chaperone competent cells for *** solubility of these recombinant proteins was determined by SDS-PAGE and Coomassie brilliant blue *** recombinant proteins were purified by NiNTA resin and identified by Western *** transduction of these proteins into HEK 293 T cells were evaluated by immunofluorescence ***：These four reprogramming proteins could be produced in soluble format in p Cold-SUMO expression vector system with the assistance of chaperone proteins in *** proteins were purified successfully with a purity of over 70% with a relative high transduction rate into 293 ***：The results in the present study indicate the four important reprogramming proteins,c Myc,Klf4,Oct4,and Sox2,can be produced in soluble format in bacteria with low *** new method thus might be expected to greatly contribute to the future study of i PSCs.

关键词： reprogramming proteins induced pluripotent stem cells soluble expression protein transduction

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36MnVS4连杆裂解加工模拟及缺陷分析

吉林大学学报（工学版） 2017年第3期47卷 861-868页

作者：寇淑清宋玮峰石舟吉林大学辊锻工艺研究所长春130022

针对具有更高疲劳强度的连杆新材料36MnVS4,开展了连杆裂解加工性能研究,并与C70S6材料进行对比,探究其裂解缺陷形成原因。依据新材料I型裂纹的脆性解理断裂特性,确定了连杆裂解K判据及最大主应力断裂失效准则,建立了预制切口条件下的... 详细信息

针对具有更高疲劳强度的连杆新材料36MnVS4,开展了连杆裂解加工性能研究,并与C70S6材料进行对比,探究其裂解缺陷形成原因。依据新材料I型裂纹的脆性解理断裂特性,确定了连杆裂解K判据及最大主应力断裂失效准则,建立了预制切口条件下的连杆裂解有限元模型。通过分析裂解过程中的应力集中和变形分布情况,获得不同材料的切口起裂位置和时间、切口附近及断面的塑性变形,结合裂纹扩展速度和裂解载荷,研究了容易导致的缺陷类型并评估了断口质量。结果表明:在相同几何参数和载荷下,因36MnVS4连杆较C70S6起裂位置分散、裂纹扩展不稳定、缺口敏感性高、铁素体组织多,容易导致批量生产时裂解工序缺陷增加,但裂解后大头孔失圆和断面塑性变形小。

关键词：材料合成与加工工艺 36MnVS4 连杆裂解加工数值模拟

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SOM神经网络在独立学院招生决策中的应用

计算机工程与科学 2011年第5期33卷 136-140页

作者：俸世洲周尚波重庆大学计算机学院重庆400030 重庆师范大学涉外商贸学院重庆401520

自组织映射(SOM)是一种竞争型无指导学习的神经网络方法。SOM神经网络已广泛地应用于模式聚类、模式识别、拓扑不变性映射等方面。本文利用SOM对中国31个省份进行聚类分析,建立独立学院招生决策模型。首先,选取各省份的报到率、第一志... 详细信息

自组织映射(SOM)是一种竞争型无指导学习的神经网络方法。SOM神经网络已广泛地应用于模式聚类、模式识别、拓扑不变性映射等方面。本文利用SOM对中国31个省份进行聚类分析,建立独立学院招生决策模型。首先,选取各省份的报到率、第一志愿率和人均GDP等作为SOM神经网络的输入模式;然后,用SOM进行聚类;最后,对聚类结果进行分析得出各类的生源地特征和等级。实验结果表明,利用SOM对生源地进行聚类分析是可行的、有效的,可以避开人的主观因素,更迅速客观地得到聚类结果。它为独立学院编制招生计划和招生宣传方案提供了一种新的参考依据,在独立学院招生领域具有较好的应用前景。

关键词：神经网络 SOM Kohonen 招生

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关联规则在独立学院招生决策中的应用

计算机工程与科学 2012年第1期34卷 119-123页

作者：俸世洲周尚波重庆大学计算机学院重庆400030 重庆师范大学涉外商贸学院重庆401520

关联规则(Association Rule)是数据挖掘领域中一个重要的研究课题,广泛应用于各个领域,既可以检验行业内长期形成的知识模式,也能够发现隐藏的新规律。本文利用关联规则对独立学院招生信息进行分析,建立独立学院招生决策模型。首先选取... 详细信息

关联规则(Association Rule)是数据挖掘领域中一个重要的研究课题,广泛应用于各个领域,既可以检验行业内长期形成的知识模式,也能够发现隐藏的新规律。本文利用关联规则对独立学院招生信息进行分析,建立独立学院招生决策模型。首先选取考生高考志愿表中的专业信息,然后利用关联规则进行挖掘,最后对挖掘出的规则进行分析和应用。实验结果表明,利用关联规则对考生高考志愿信息进行挖掘是可行的、有效的,它为独立学院编制招生计划和制定招生宣传方案提供了一种新的参考依据,在独立学院招生领域具有较好的应用前景。

关键词：数据挖掘关联规则招生

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水力裂缝与天然裂缝相互作用与影响

科学技术与工程 2016年第36期16卷 13-19页

作者：孙博薛世峰周博中国石油大学(华东)储运与建筑工程学院青岛266580

在非常规油气藏中,复杂裂缝网络的形成在很大程度上受水力裂缝与天然裂缝相互作用的影响。基于断裂力学理论,建立了水力裂缝与天然裂缝相互作用力学模型,分析天然裂缝介质系统中水力裂缝遭遇天然裂缝后的扩展形态。对于水力裂缝穿越天... 详细信息

在非常规油气藏中,复杂裂缝网络的形成在很大程度上受水力裂缝与天然裂缝相互作用的影响。基于断裂力学理论,建立了水力裂缝与天然裂缝相互作用力学模型,分析天然裂缝介质系统中水力裂缝遭遇天然裂缝后的扩展形态。对于水力裂缝穿越天然裂缝的情况,给出了穿越角度;对于未穿越情况,给出天然裂缝张开闭合形态判别方法。通过数值计算研究了水平主应力差、逼近角、界面摩擦系数和裂缝净压力等因素的影响。结果表明,在高水平主应力差、高逼近角和高界面摩擦的条件下,水力裂缝倾向于穿越天然裂缝;在低水平主应力差、低逼近角和低界面摩擦的条件下,水力裂缝则更容易被捕获。同时,在水力裂缝内净压力越高,天然裂缝越容易张开。该模型为分析水力裂缝与天然裂缝相互作用过程以及水力压裂工程设计提供了依据。

关键词：水力裂缝水力与天然裂缝相互作用裂缝穿越与捕获张开与闭合状态

Bioinspired Passive Tactile Sensors Enabled by Reversible Polarization of Conjugated Polymers

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Nano-Micro Letters 2025年第1期17卷 361-377页

作者： feng He Sitong Chen Ruili zhou Hanyu Diao Yangyang Han Xiaodong Wu School of Mechanical Engineering Sichuan UniversityChengdu 610065People’s Republic of China School of Software Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065People’s Republic of China State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengdu 610065People’s Republic of China

Tactile perception plays a vital role for the human body and is also highly desired for smart prosthesis and advanced *** to active sensing devices,passive piezoelectric and triboelectric tactile sensors consume less power,but lack the capability to resolve static ***,we address this issue by utilizing the unique polarization chemistry of conjugated polymers for the first time and propose a new type of bioinspired,passive,and bio-friendly tactile sensors for resolving both static and dynamic ***,to emulate the polarization process of natural sensory cells,conjugated polymers(including poly(3,4-ethylenedioxythiophen e):poly(styrenesulfonate),polyaniline,or polypyrrole)are controllably polarized into two opposite states to create artificial potential *** controllable and reversible polarization process of the conjugated polymers is fully in situ ***,a micro-structured ionic electrolyte is employed to imitate the natural ion channels and to encode external touch stimulations into the variation in potential difference *** with the currently existing tactile sensing devices,the developed tactile sensors feature distinct characteristics including fully organic composition,high sensitivity(up to 773 mV N^(−1)),ultralow power consumption(nW),as well as superior *** demonstrations,both single point tactile perception(surface texture perception and material property perception)and two-dimensional tactile recognitions(shape or profile perception)with high accuracy are successfully realized using self-defined machine learning *** tactile sensing concept innovation based on the polarization chemistry of conjugated polymers opens up a new path to create robotic tactile sensors and prosthetic electronic skins.

关键词： Passive tactile sensors Reversible polarization of conjugated polymers Tactile perception Machine learning algorithm Object recognition

人心肌型脂肪酸结合蛋白在大肠杆菌中的可溶性表达和纯化

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药物生物技术 2018年第1期25卷 7-11页

作者：钟丽施建丰周竞马小力江苏省中西医结合医院检验科江苏南京210028

人心脏型脂肪酸结合蛋白(Heart type-fatty acid binding protein,H-FABP)是存在于心肌细胞质内的小分子可溶性蛋白质,占心脏全部可溶性蛋白的4%~8%,具有很强的器官特异性。在急性心肌梗塞(AMI)早期,心肌细胞中H-FABP大量增加,因此可将... 详细信息

人心脏型脂肪酸结合蛋白(Heart type-fatty acid binding protein,H-FABP)是存在于心肌细胞质内的小分子可溶性蛋白质,占心脏全部可溶性蛋白的4%~8%,具有很强的器官特异性。在急性心肌梗塞(AMI)早期,心肌细胞中H-FABP大量增加,因此可将其作为急性心肌梗塞等心肌损伤的生化标志物。大肠杆菌是常用的可快速生产重组蛋白的宿主,且融合或嵌合"标签"蛋白连接靶蛋白不仅能提高靶蛋白的溶解性,同时也可以防止细胞内的蛋白水解。作者旨在获得重组可溶性人心肌型脂肪酸结合蛋白(H-FABP),为进一步开发应用奠定基础。选择麦芽糖结合蛋白(MBP)作为H-FABP的融合标签蛋白,以提高H-FABP在大肠杆菌中的可溶性表达能力。通过RT-PCR扩增人H-FABP的基因序列,将目的基因克隆入pET28a表达载体,转化到DH5α感受态细菌中扩增得到原核表达重组质粒pET28a-MBP-H-FABP。将质粒转化入大肠杆菌BL21中,通过IPTG诱导,表达MBPH-FABP融合蛋白,同时SDS-PAGE分析验证。后经镍固定金属亲和层析纯化及离子亲和纯化法纯化,并经SDSPAGE和免疫印迹分析鉴定纯化后重组蛋白的免疫特异性。SDS-PAGE和免疫印迹分析显示重组蛋白相对分子质量约为55 k。经Ni^(+2)-NTA纯化后,MBP-H-FABP重组蛋白在SDS-PAGE电泳下获得单一条带,且MBP-H-FABP可与商业化的H-FABP单克隆抗体(Clone 12-1)呈特异性反应。其意义在于提供在大肠杆菌中制备可溶性重组人H-FABP的方法,为研究其生物学功能和制备单克隆抗体以及今后进行大规模的生产奠定了基础。

关键词：心肌型脂肪酸结合蛋白麦芽糖结合蛋白表达纯化大肠杆菌免疫特异性

A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2025年第2期20卷 503-517页

作者： Qi Liu Jianye Xie Runxue zhou Jin Deng Weihong Nie Shuwei Sun Haiping Wang Chunying shi Department of Human Anatomy Histology and EmbryologySchool of Basic MedicineQingdao UniversityQingdaoShandong ProvinceChina Department of Neurology The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Department of General Practice The Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic ***,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in ***,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic *** metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ***,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel ***-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in *** results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor *** findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

关键词： angiogenesis biomaterial blood-brain barrier cerebral ischemia/reperfusion injury control release drug delivery inflammation QK peptides matrix metalloproteinase-2 neuroprotection self-assembling nanofiber hydrogel