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Analysis of multi-objective optimisation method for main insulation structure configuration scheme of valve-side winding of ultra-high voltage converter transformer considering multi-field coupling

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High Voltage 2024年第2期9卷 296-308页

作者： Lijun Yang Zhidong cheng Li cheng Ruijin Liao State Key Laboratory of Power Transmission Equipment Technology School of Electrical EngineeringChongqing UniversityChongqingChina

Because DC conductivities of oil-paper materials can be easily affected by electric field and temperature,the electrical properties under electro-thermal coupling of oil-paper materials should be considered when optimising the insulation structure of valve-side winding of the converter *** on the finite element multi-physical field coupling simulation,the multi-objective optimisation of main insulation structure of valve-side winding is carried *** view of the high time consuming of multi-physical field calculation and the actual needs of an insulation structure optimisation,the Kriging method and technique for order preference by similarity to the ideal solution(TOPSIS)method are introduced,and combines them with NSGA-II to obtain a joint algorithm that can be used for the optimisation of insulation structure of the converter *** using the joint algorithm of multi-objective optimisation,the insulation structure configuration scheme of valve-side winding is *** can be seen from the experiment result that,on the one hand,the joint algorithm adopted can obtain the insulation structure configuration scheme that meets the design *** the other hand,when facing the configuration scheme optimisation under multi-field coupling,the computational time required by this method is only 0.15%of that required by original genetic algorithm.

关键词： converter insulation coupling

Semisynthesis of homogeneous spike RBD glycoforms from SARS-CoV-2 for profiling the correlations between glycan composition and function

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National Science Review 2024年第2期11卷 145-154页

作者： Farong Ye cheng Li Feng-Liang Liu Xinliang Liu Peng Xu Rong-Hua Luo Wenping Song Yong-Tang Zheng Tianlei Ying Biao yu Ping Wang Center for Chemical Glycobiology Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study School of Chemistry and Chemical Engineering Shanghai Jiao Tong University MOE/NHC/CAMS Key Laboratory of Medical Molecular Virology Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection Shanghai Institute of Infectious Disease and BiosecurityShanghai Engineering Research Center for Synthetic ImmunologySchool of Basic Medical Sciences Shanghai Medical College Fudan University Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of yunnan Province Center for Biosafety Mega-Science Kunming Institute of ZoologyChinese Academy of Sciences State Key Laboratory of Bioorganic and Natural Products Chemistry Center for Excellence in Molecular Synthesis Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shenzhen Research Institute of Shanghai Jiao Tong University

Vaccines have been the primary remedy in the global fight against coronavirus disease 2019（COVID-19）.The receptor-binding domain（RBD） of the spike protein, a critical viral immunogen, is affected by the heterogeneity of its glycan structures and relatively low immunogenicity. Here, we describe a scalable synthetic platform that enables the precise synthesis of homogeneously glycosylated RBD, facilitating the elucidation of carbohydrate structure–function relationships. Five homogeneously glycosylated RBDs bearing biantennary glycans were prepared, three of which were conjugated to T-helper epitope(Tpep) from tetanus toxoid to improve their weak immune response. Relative to natural HEK293-derived RBD, synthetic RBDs with biantennary N-glycan elicited a higher level of neutralising antibodies against SARS-CoV-2 in mice. Furthermore, RBDs containing Tpepelicited significant immune responses in transgenic mice expressing human angiotensin-converting enzyme 2. Our collective data suggest that trimming the N-glycans and Tpepconjugation of RBD could potentially serve as an effective strategy for developing subunit vaccines providing efficient protection.

关键词： peptide ligation glycosylation glycoprotein

Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection

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Chinese Chemical Letters 2024年第6期35卷 531-535页

作者： Jia-Li Xie Tian-Jin Xie yu-Jie Luo Kai Mao cheng-Zhi Huang yuan-Fang Li Shu-Jun Zhen Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University) Ministry of EducationCollege of Chemistry and Chemical EngineeringSouthwest UniversityChongqing 400715China Key Laboratory of Luminescent and Real-Time Analytical Chemistry(Southwest University) Chongqing Science and Technology BureauCollege of Pharmaceutical SciencesSouthwest UniversityChongqing 400715China

Fluorescence Anisotropy(FA)is an effective biochemical detection method based on molecular *** oxide(GO)has been extensively used as an FA ***,the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA *** order to address these problems,an octopus-like DNA nanostructure(ODN)was designed and coupled with GO to enhance the FA together in this *** mimicking the multi-clawed structure of the octopus,the ODN can be adsorbed on GO tightly,which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN,but also could improve the specificity due to the decrease of the nonspecific interaction in complex ***,ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO,which could improve the *** method has been applied for the detection of hepatitis B virus DNA(HBV-DNA)in a range of 1-50 nmol/L and the limit of detection(LOD)was 330 pmol/*** addition,the proposed method has been successfully utilized to detect HBV-DNA in human serum,indicating that this method has a great practical application prospect.

关键词： Fluorescence anisotropy Graphene oxide(GO) Octopus-like DNA nanostructure HBV-DNA

Huangqin Decoction Delays Progress of Colitis-Associated Carcinogenesis by Regulating Nrf2/HO-1 Antioxidant Signal Pathwayin Mice

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Chinese Journal of Integrative Medicine 2024年第2期30卷 135-142页

作者： GU Li-mei LI He-zhong GAO Lei LI Hui WEI Lan-fu PAN cheng-yu WU Ke-xuan TIAN Yao-zhou Department of Gastrointestinal Endoscopy Affiliated Hospital of Nanjing University of Chinese MedicineNanjing(210029)China Department of Gastroenterology Affiliated Hospital of Integrated Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing(210028)China School of Integrated Chinese and Western Medicine Nanjing University of Chinese MedicineNanjing(210023)China

Objective:To investigate the effect of Huangqin Decoction(HQD)on nuclear factor erythroid 2 related-factor 2(Nrf2)/heme oxygenase(HO-1)signaling pathway by inducing the colitis-associated carcinogenesis(CAC)model mice with azoxymethane(AOM)/dextran sodium sulfate(DSS).Methods:The chemical components of HQD were analyzed by liquid chromatography-quadrupole-time-of-flight mass spectrometry(LC-Q-TOF-MS/MS)to determine the molecular constituents of *** 48 C57BL/6J mice were randomly divided into 6 groups by a random number table,including control,model(AOM/DSS),mesalazine(MS),low-,medium-,and high-dose HQD(HQD-L,HQD-M,and HQD-H)groups,8 mice in each *** for the control group,the mice in the other groups were intraperitoneally injected with AOM(10 mg/kg)and administrated with 2.5%DSS orally for 1 week every two weeks(totally 3 rounds of DSS)to construct a colitis-associated carcinogenesis mouse *** mice in the HQD-L,HQD-M and HQD-H groups were given HQD by gavage at doses of 2.925,5.85,and 11.7 g/kg,respectively;the mice in the MS group was given a suspension of MS at a dose of 0.043 g/kg(totally 11 weeks).The serum levels of malondialdehyde(MDA)and superoxide dismutase(SOD)were measured by enzyme-linked immunosorbent *** mRNA and protein expression levels of Nrf2,HO-1,and inhibitory KELCH like ECH-related protein 1(Keap1)in colon tissue were detected by quantitative real-time PCR,immunohistochemistry,and Westem blot,***:LC-Q-TOF-MS/MS analysis revealed that the chemical constituents of HQD include baicalin,paeoniflorin,and glycyrrhizic *** to the control group,significantly higher MDA levels and lower SOD levels were observed in the model group(P<0.05),whereas the expressions of Nrf2 and HO-1 were significantly decreased,and the expression of Keap1 increased(P<0.01).Compared with the model group,serum MDA level was decreased and SOD level was increased in the HQD-M,HQD-H and MS groups(P<0.05).Higher expressions of

关键词： Huangqin Decoction colitis-associated carcinogenesis nuclear factor erythroid 2 related-factor 2/heme oxygenase signaling pathway oxidative stress Chinese medicine

Preoperative serum cortisone levels are associated with cognition in preschool‑aged children with tetralogy of Fallot after corrective surgery:new evidence from human populations and mice

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World Journal of Pediatrics 2024年第2期20卷 173-184页

作者： Si-yu Ma yu-Ting Liu yue-Shuang Cun Qiang Wang Ming-Cui Fu Ke-De Wu Xin-yu Cai Shu-Ting cheng Nishant Patel Min Da Liang Hu Zhuoga Deqin Xue-Jun Kang Ming Yang Xu-Ming Mo Department of Cardiothoracic Surgery Children’s Hospital of Nanjing Medical University72 Guangzhou RoadNanjing 210008China Department of Radiology Children’s Hospital of Nanjing Medical University72 Guangzhou RoadNanjing 210008China Key Laboratory of Child Development and Learning Science Research Center For Learning ScienceSchool of Biological Sciences&Medical EngineeringMinistry of EducationSoutheast UniversityNanjing 210096China Medical School of Nanjing University Nanjing 210093China

Background Tetralogy of Fallot(TOF)is the most common cyanotic congenital heart *** with TOF would be confronted with neurological impairment across their *** study aimed to identify the risk factors for cerebral morphology changes and cognition in postoperative preschool-aged children with *** We used mass spectrometry(MS)technology to assess the levels of serum metabolites,Wechsler preschool and primary scale of intelligence-Fourth edition(WPPSI-Ⅳ)index scores to evaluate neurodevelopmental levels and multimodal magnetic resonance imaging(MRI)to detect cortical morphological *** Multiple linear regression showed that preoperative levels of serum cortisone were positively correlated with the gyrification index of the left inferior parietal gyrus in children with TOF and negatively related to their lower visual spaces index and nonverbal ***,preoperative SpO_(2) was negatively correlated with levels of serum cortisone after adjusting for all ***,after intervening levels of cortisone in chronic hypoxic model mice,total brain volumes were reduced at both postnatal(P)11.5 and P30 *** Our results suggest that preoperative serum cortisone levels could be used as a biomarker of neurodevelopmental impairment in children with *** study findings emphasized that preoperative levels of cortisone could influence cerebral development and cognition abilities in children with TOF.

关键词： Cognition Cortisone MRI Neurodevelopment Tetralogy of Fallot

Simple and versatile in situ thermo-sensitive hydrogel for rectal administration of SZ-A to alleviate inflammation and repair mucosal barrier in ulcerative colitis

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Chinese Chemical Letters 2024年第6期35卷 462-468页

作者： yu Yan Jiawei Song Dongdong Liu Zihan Liu Jialing cheng Zhiyang Chen Yanfang Yang Weizhe Jiang Hongliang Wang Jun Ye yuling Liu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China College of Pharmacy Guangxi Medical UniversityNanning 530021China Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

Ulcerative colitis(UC)is a chronic inflammatory bowel disease characterized by persistent inflammation of the colon and disrupted intestinal *** mori(Sangzhi)alkaloids(SZ-A),derived from twigs of mulberry,were approved by the National Medical Products Administration in 2020 for treating type 2 diabetes *** evidence has confirmed that SZ-A also alleviates non-alcoholic fatty liver disease and ameliorates inflammation,indicating its potential to address inflammation in ***,the treatment of UC faces challenges due to low drug delivery efficiency and short retention *** overcome these challenges,an injectable and adherent in-situ thermo-sensitive hydrogel containing SZ-A was developed for rectal drug delivery,utilizing the thermo-sensitive polymers Poloxamer 407and *** thermo-sensitive hydrogel system was designed with a moderate gelation temperature of 32±0.5℃,a short gelation time of 64 s,a p H range of 7-10,high moisturizing capability exceeding 90%,and moderate mechanical strength of 4-5 *** a rat model with UC,the in situ thermo-sensitive hydrogel significantly extended the retention time at the colonic site and enabled sustained release after rectal *** of UC were markedly reduced following rectal administration of SZ-A thermosensitive ***,the release of inflammatory factors,such as interleukin-1β(IL-1β),IL-6,IL-18,tumor necrosis factor-α(TNF-α),and transforming growth factor-β1(TGF-β1),significantly decreased in the SZ-A thermo-sensitive hydrogel *** integrity of the colonic mucosal barrier was significantly enhanced following the application of SZ-A thermo-sensitive *** conclusion,rectal administration of SZ-A in situ thermo-sensitive hydrogel effectively alleviated UC symptoms,inhibited the secretion of inflammatory factors,and promoted the repair of the colonic mucosal *** approach holds promise as a potential treatment for UC.

关键词： Ulcerative colitis SZ-A Thermo-sensitive hydrogel Rectal administration Alleviation of inflammation Mucosal barrier repair

Fecal microbiota transplantation for treatment of non-alcoholic fatty liver disease:Mechanism,clinical evidence,and prospect

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World Journal of Gastroenterology 2024年第8期30卷 833-842页

作者： Xiao-Xia Qiu Sheng-Li cheng Yan-Hui Liu yu Li Rui Zhang Nan-Nan Li Zheng Li Research and Education Department Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Anhui Provincial Hospital The First Affiliated Hospital of University of Science and Technology of ChinaHefei 230000Anhui ProvinceChina Department of Clinical Pharmacy Anhui Provincial Children’s HospitalHefei 230000Anhui ProvinceChina Department of Pharmacy Taihe County People’s Hospital of Anhui ProvinceFuyang 236600Anhui ProvinceChina Department of Pharmacy The Second People’s Hospital of HefeiHefei Hospital Affiliated to Anhui Medical UniversityHefei 230000Anhui ProvinceChina University of Science and Technology of China The First Affiliated Hospital of University of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Jiangsu Engineering Research Center of Cardiovascular Drugs Targeting Endothelial Cells College of Health SciencesSchool of Life SciencesJiangsu Normal UniversityXuzhou 221000Jiangsu ProvinceChina

The population of non-alcoholic fatty liver disease(NAFLD)patients along with relevant advanced liver disease is projected to continue growing,because currently no medications are approved for *** microbiota transplantation(FMT)is believed a novel and promising therapeutic approach based on the concept of the gut-liver axis in liver *** has been an increase in the number of pre-clinical and clinical studies evaluating FMT in NAFLD treatment,however,existing findings diverge on its ***,we briefly summarized the mechanism of FMT for NAFLD treatment,reviewed randomized controlled trials for evaluating its efficacy in NAFLD,and proposed the prospect of future trials on FMT.

关键词： Non-alcoholic fatty liver disease Fecal microbiota transplantation Randomized controlled trial Mechanism Efficacy

Achieving laparoscopic anatomic resection for hepatocellular carcinoma by fluorescence guided positive staining of hepatic cone unit

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Hepatobiliary & Pancreatic Diseases International 2024年第4期23卷 406-411页

作者： Jun-Fang Deng Chuan-Hui Peng yu Zhang Xiao-Feng Xu Rong-Liang Tong Zheng-Long Zhai Di-yu Chen cheng Zhang Li-Ming Wu Jian Wu Division of Hepatobiliary Pancreatic Surgery the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Laparoscopic anatomical liver resection has been increasingly reported worldwide,but how to demarcate the resection planes is technically challenging[1].Positive staining technique with intraoperative indocyanine green(ICG)-fluorescence imaging has been introduced as an effective tool for real-time intraoperative guidance[2].Liver anatomy is ***,liver is composed of 9 segments,each is composed of two to three subsegments,and each subsegment contains several hepatic cone units.A hepatic cone unit is dominated by a tertiary or quaternary hepatic pedicles[3].

关键词： hepatic resection anatomy

Amlodipine inhibits the proliferation and migration of esophageal carcinoma cells through the induction of endoplasmic reticulum stress

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World Journal of Gastroenterology 2024年第4期30卷 367-380页

作者： Yan-Min Chen Wen-Qian Yang cheng-Wei Gu Ying-Ying Fan yu-Zhen Liu Bao-Sheng Zhao Department of Thoracic Surgery The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Department of Oncology The Affiliated HospitalHenan Polytechnic UniversityJiaozuo 454000Henan ProvinceChina Life Science Research Center The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina Department of Gastroenterology The First Affiliated Hospital of Xinxiang Medical UniversityWeihui 453100Henan ProvinceChina

BACKGROUND L-type calcium channels are the only protein channels sensitive to calcium channel blockers,and are expressed in various cancer *** Cancer Genome Atlas database shows that the mRNA levels of multiple L-type calcium channel subunits in esophageal squamous cell carcinoma tumor tissue are significantly higher than those in normal esophageal epithelial ***,we hypothesized that amlodipine,a long-acting dihydropyridine L-type calcium channel blocker,may inhibit the occurrence and development of esophageal cancer(EC).AIM To investigate the inhibitory effects of amlodipine on EC through endoplasmic reticulum(ER)*** Cav1.3 protein expression levels in 50 pairs of EC tissues and corresponding paracancerous tissues were ***,the inhibitory effects of amlodipine on proliferation and migration of EC cells in vitro were detected using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and Transwell *** vivo experiments were performed using murine xenograft *** elucidate the underlying mechanisms,in vitro cell studies were performed to confirm that ER stress plays a role in inhibition proliferation and migration of EC cells treated with *** The expression level of Cav1.3 in esophageal carcinoma was 1.6 times higher than that in paracancerous *** treatment decreased the viability of esophageal carcinoma cells in a dose-and time-dependent *** vivo animal experiments also clearly indicated that amlodipine inhibited the growth of EC tumors in ***,amlodipine reduces the migration of tumor cells by inhibiting epithelial-mesenchymal transition(EMT).Mechanistic studies have demonstrated that amlodipine induces ER stress-mediated apoptosis and suppresses ***,amlodipine-induced autophagy was characterized by an increase in autophagy lysosomes and the accumulation of light chain 3B *** combination of amlodipine with the ER stress inhibitor 4-p

关键词： L-type calcium channel Amlodipine Esophageal cancer Autophagy Endoplasmic reticulum stress