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Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1092-1105页

作者： Qian Zhao Longqing shi Weiyi He Jinyu li shijun you Shuai Chen Jing lin Yibin Wang liwen Zhang Guang Yang liette Vasseur Minsheng you State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Rice Fujian Academy of Agricultural SciencesFuzhou 350018China Tea Research Institute Fujian Academy of Agricultural SciencesFuzhou 350001China Center for Genomics and Biotechnology Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biological Sciences Brock UniversitySt.CatharinesON L2S 3A1Canada

Tea green leafhopper(TGL),Empoasca onukii,is of biological and economic *** numerous studies,the mechanisms underlying its adaptation and evolution remain ***,we use previously untapped genome and population genetics approaches to examine how the pest adapted to different environmental variables and thus has expanded *** complete a chromosome-level assembly and annotation of the *** genome,showing notable expansions of gene families associated with adaptation to chemoreception and *** signals indicating balancing selection highlight metabolic pathways involved in adaptation to a wide range of tea varieties grown across ecologically diverse *** of genetic variations among 54 *** samples unveil the population structure and evolutionary history across different tea-growing regions in *** results demonstrate that the genomic changes in key pathways,including those linked to metabolism,circadian rhythms,and immune system functions,may underlie the successful spread and adaptation of *** work highlights the genetic and molecular basis underlying the evolutionary success of a species with broad economic impacts,and provides insights into insect adaptation to host plants,which will ultimately facilitate more sustainable pest management.

关键词： Tea green leafhopper Genomic variation Population genetics Local adaptation Evolutionary history

Intercellular mitochondrial transfer as a means of revitalizing injured glomerular endothelial cells

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World Journal of Stem Cells 2022年第9期14卷 729-743页

作者： li-Xia Tang Bing Wei Lu-Yao Jiang you-you Ying Ke li Tian-Xi Chen Ruo-Fei Huang Miao-Jun shi Hang Xu Department of Endocrinology The First People’s Hospital of Yongkang Affiliated to Hangzhou Medical CollegeJinhua 321300Zhejiang ProvinceChina School of Medicine Southeast UniversityNanjing 210009Jiangsu ProvinceChina Department of Medical Rehabilitation The First People’s Hospital of Yongkang Affiliated to Hangzhou Medical CollegeJinhua 321300Zhejiang ProvinceChina Department of Nephrology The First People’s Hospital of Yongkang Affiliated to Hangzhou Medical CollegeJinhua 321300Zhejiang ProvinceChina Department of Hemodialysis/Nephrology The First People’s Hospital of Yongkang Affiliated to Hangzhou Medical CollegeJinhua 321300Zhejiang ProvinceChina

BACKGROUND Recent studies have demonstrated that mesenchymal stem cells(MSCs)can rescue injured target cells via mitochondrial ***,it has not been fully understood how bone marrow-derived MSCs repair glomeruli in diabetic kidney disease(DKD).AIM To explore the mitochondrial transfer involved in the rescue of injured glomerular endothelial cells(GECs)by MSCs,both in vitro and in *** In vitro experiments were performed to investigate the effect of co-culture with MSCs on high glucose-induced *** transfer of mitochondria was visualized using fluorescent *** were freshly sorted and ultimately tested for apoptosis,viability,mRNA expression by real-time reverse transcriptase-polymerase chain reaction,protein expression by western blot,and mitochondrial ***,streptozotocin-induced DKD rats were infused with MSCs,and renal function and oxidative stress were detected with an automatic biochemical analyzer and related-detection kits after 2 *** histology was analyzed by hematoxylin and eosin,periodic acid-Schiff,and immunohistochemical *** Fluorescence imaging confirmed that MSCs transferred mitochondria to injured GECs when cocultured in *** found that the apoptosis,proliferation,and mitochondrial function of injured GECs were improved following ***,MSCs decreased pro-inflammatory cytokines[interleukin(IL)-6,IL-1β,and tumor necrosis factor-α]and pro-apoptotic factors(caspase 3 and Bax).Mitochondrial transfer also enhanced the expression of superoxide dismutase 2,B cell lymphoma-2,glutathione peroxidase(GPx)3,and mitofusin 2 and inhibited reactive oxygen species(ROS)and dynamin-related protein 1 ***,MSCs significantly ameliorated functional parameters(blood urea nitrogen and serum creatinine)and decreased the production of malondialdehyde,advanced glycation end products,and ROS,whereas they increased the levels of GPx and superoxide dismutase in *** addition,

关键词： Mitochondria transfer Mesenchymal ste

减氮及有机肥替代对旱地冬小麦干物质积累、转运、分配和产量的影响

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作物学报 2025年第1期51卷 207-220页

作者：张军胡川周起晖任开明董誓言刘傲寒吴金芝黄明李友军河南科技大学农学院河南洛阳471023 商洛学院生物医药与食品工程学院陕西商洛726000

探究减氮及有机肥替代对旱地冬小麦产量形成和经济效益的影响,为实施化肥减量和有机肥替代提供理论和技术依据。2021-2023年度,基于2019年开始设置在黄土高原和黄淮海平原交汇处典型旱地冬小麦产区(河南洛宁和河南孟津)的定位试验,研究... 详细信息

探究减氮及有机肥替代对旱地冬小麦产量形成和经济效益的影响,为实施化肥减量和有机肥替代提供理论和技术依据。2021-2023年度,基于2019年开始设置在黄土高原和黄淮海平原交汇处典型旱地冬小麦产区(河南洛宁和河南孟津)的定位试验,研究了冬小麦干物质积累转运分配特性、氮肥干物质生产效率、产量及其构成要素和经济效益。试验设置不施氮肥(NN)、农户施氮(FN)、基于FN减氮20%(RN)和RN基础上20%氮肥用有机肥替代(OSN)4个处理。结果表明:(1)与FN相比,RN处理降低了小麦拔节期、开花期和成熟期的干物质积累量、花前干物质转运量以及成熟期茎鞘、穂轴+颖壳和籽粒的干物质分配量,但对籽粒产量无显著影响。(2)与FN和RN相比, OSN处理提高了各生育阶段的氮肥干物质生产效率,从而增加了拔节期、开花期、成熟期的干物质积累量,而且提高了花前干物质转运量、花后干物质积累量、花后干物质积累量对籽粒的贡献率,进而使成熟期地上部各器官的干物质分配量均得到显著提高,最终使籽粒产量分别显著增加15.03%和17.12%,经济效益增加3.84%和4.23%。(3)小麦产量与花前干物质转运量、花后干物质积累量和花后干物质积累量对籽粒的贡献率呈极显著正相关,与花前干物质转运量对籽粒的贡献率呈极显著负相关。综上,在雨养条件下,小麦季施氮量为172kghm^(–2) (夏休闲-冬小麦)和192kg hm^(–2) (夏玉米-冬小麦)的基础上,OSN处理提高了氮肥干物质生产效率,增加各生育时期的干物质积累量,其花前干物质转运量和花后干物质积累量的协同增加使其获得了最高产量,实现增产增收,适宜在产量水平为5000kghm^(–2)的雨养旱地冬小麦高产栽培中应用。

关键词：旱地冬小麦减氮有机肥替代干物质积累转运产量经济效益

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Time-resolved Luminescence-based Chemosensor for Fluoride Anion

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Chinese Chemical Letters 2006年第1期17卷 69-72页

作者： Mei shi Xiang Hong li Fu you li Deng Qing ZHANG Bing Bing DAI Tao YI Chun Hui HUANG Laboratory of Advanced Materials Fudan University Shanghai 200433

A new europium complex is descried as a time-resolved luminescence-based sensor for fluoride anion. The sensor is selective even in the presence of intensive background fluorescence.

关键词： Fluorescent chemosensor time-resolved luminescence fluoride anion.

Efficient matrix inversion based on VliW architecture

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Journal of Systems Engineering and Electronics 2014年第3期25卷 393-398页

作者： li Zhang Fu li Guangming shi School of Electronic Engineering Xidian University

Matrix inversion is a critical part in communication, signal processing and electromagnetic system. A flexible and scalable very long instruction word （VliW） processor with clustered architecture is proposed for matrix inversion. A global register file （RF） is used to connect al the clusters. Two nearby clusters share a local register file. The instruction sets are also designed for the VliW processor. Experimental results show that the proposed VliW architecture takes only 45 latency to invert a 4 × 4 matrix when running at 150 MHz. The proposed design is roughly five times faster than the DSP solution in processing speed.

关键词： matrix inversion very long instruction word (VliW),latency register file (RF) cluster.

Magnon squeezing enhanced ground-state cooling in cavity magnomechanics

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Fundamental Research 2023年第1期3卷 3-7页

作者： M.Asjad Jie li shi-Yao Zhu J.Q.you Department of Applied Mathematics and Sciences Khalifa UniversityAbu Dhabi 127788United Arab Emirates Interdisciplinary Center of Quantum Information State Key Laboratory of Modern Optical Instrumentationand Zhejiang Province Key Laboratory of Quantum Technology and DeviceDepartment of PhysicsZhejiang UniversityHangzhou 310027China

Cavity magnomechanics has recently become a new platform for studying macroscopic quantum *** magnetostriction induced vibration mode of a large-size ferromagnet or ferrimagnet reaching its ground state represents a genuine macroscopic quantum *** we study the ground-state cooling of the mechanical vibration mode in a cavity magnomechanical system,and focus on the role of magnon squeezing in improving the cooling *** magnon squeezing is obtained by exploiting the magnon self-Kerr *** find that the magnon squeezing can significantly and even completely suppress the magnomechanical Stokes *** thus becomes particularly useful in realizing ground-state cooling in the unresolved-sideband regime,where the conventional sideband cooling protocols become *** also find that the coupling to the microwave cavity plays only an adverse effect in mechanical *** makes essentially the two-mode magnomechanical system(without involving the microwave cavity)a preferred system for cooling the mechanical motion,in which the magnon mode is established by a uniform bias magnetic field and a microwave drive field.

关键词： Cavity magnomechanics Ground-state cooling Magnetostriction Optomechanics Dispersive coupling

Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第7期11卷 524-530页

作者： Chun-mao HAN li-ping ZHANG Jin-zhang SUN Hai-fei shi Jie ZHOU Chang-you GAO Department of Burn the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China

To create a scaffold that is suitable for the construction of tissue-engineered skin, a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen-chitosan porous membrane with fibrin glue. Tissue-engineered skin was fabricated using this asymmetric scaffold, fibroblasts, and a human keratinocyte line (HaCaT). Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold. Scanning electron microscopy showed typical shuttle-like fibroblasts adhering to the wall of the scaffold and fluorescence microscopy showed them growing in the dermal layer of the scaffold. The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. The results of our study suggest that the asymmetric scaffold is a promising biologically functional material for skin tissue engineering, with prospects for clinical applications.

关键词： Collagen Chitosan Fibrin glue Scaffold Tissue-engineered skin

Heavy ion‑induced MCUs in 28nm SRAM‑based FPGAs:upset proportions,classifications,and pattern shapes

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Nuclear Science and Techniques 2022年第12期33卷 128-137页

作者： Shuai Gao Xin‑Yu li shi‑Wei Zhao Ze He Bing Ye li Cai you‑Mei Sun Guo‑Qing Xiao Chang Cai Jie liu Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China University of Chinese Academy of Sciences Beijing 100049China School of Physics and Electronic Information Engineering Jining Normal UniversityUlanqab 012000China State Key Laboratory of ASIC and System Fudan UniversityShanghai 201203China

For modern scaling devices,multiple cell upsets(MCUs)have become a major threat to high-reliability field-programmable gate array(FPGA)-based ***,both performing the worst-case irradiation tests to provide the actual MCU response of devices and proposing an effective MCU distinction method are urgently *** this study,high-and medium-energy heavy-ion irradiations for the configuration random-access memory of 28 nm FPGAs are *** MCU extraction method supported by theoretical predictions is proposed to study the MCU sizes,shapes,and frequencies in *** on the extraction method,the different percentages,and orientations of the large MCUs in both the azimuth and zenith directions determine the worse irradiation response of the *** extracted largest 9-bit MCUs indicate that high-energy heavy ions can induce more severe failures than medium-energy *** results show that both the use of high-energy heavy ions during MCU evaluations and effective protection for the application of high-density 28 nm FPGAs in space are extremely necessary.

关键词： FPGAs Heavy ions Multiple cell upsets Extraction Worse irradiation

A survey on the off-target effects of insecticidal double-stranded RNA targeting the Hvβ'COPI gene in the crop pest Henosepilachna vigintioctopunctata through RNA-seq

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Journal of Integrative Agriculture 2022年第9期21卷 2665-2674页

作者： Lü Jing Satyabrata NANDA CHEN shi-min MEI Yang HE Kang QIU Bao-li ZHANG you-jun li Fei PAN Hui-peng Key Laboratory of Bio-pesticide Innovation and Application of Guangdong Province South China Agricultural UniversityGuangzhou 510642P.R.China MS Swaminathan School of Agriculture Centurion University of Technology and ManagementParalakhemundi 761211India Institute of Insect Sciences College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058P.R.China Department of Plant Protection Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081P.R.China

The specificity of the double-stranded RNA(dsRNA) used in the RNA interference(RNAi) technique is crucial for the success of sequence-specific gene silencing. Currently, RNAi-mediated insect control is a trending research ***, the off-target effects of the dsRNA in RNAi are a major concern. In this study, the ds Hvβ’COPI(coat protein complex I, β’ subunit)-treated and untreated transcriptomes of the 28-spotted potato lady beetle(Henosepilachna vigintioctopunctata) were compared to understand its off-target gene silencing effects. The RNA-seq results revealed that 63 and 44 differentially expressed genes(DEGs) were upregulated and downregulated, respectively, in the ds Hvβ’COPI treated group as compared with the control. Validation of the differential expressions of some selected DEGs via reverse transcription-quantitative PCR(RT-qPCR) analysis confirmed the reliability of the transcriptome analysis results. Further downstream analysis revealed that there were no genes homologous with Hvβ’COPI in H. vigintioctopunctata. Additionally,no genes with a >11 bp continuous match with ds Hvβ’COPI were found in the H. vigintioctopunctata transcriptome. Six genes(Hvcitron, Hvhelicase, Hvtransposase, Hvserine, Hvdynein, and Hv E3 ubiquitin) were selected to examine the offtarget activity of ds Hvβ’COPI based on their potential involvement in various H. vigintioctopunctata metabolic *** severity of silencing these six off-target genes was evaluated by employing RNAi. The RNAi results confirmed the downregulation of the expression of all six genes, although there was no significant lethality. The findings of this study will be helpful in the risk analysis of future RNAi-mediated pest control experiments.

关键词： RNAi dsRNA off-target effects RNA-seq transcriptome analysis