检索结果-南通市图书馆

Ca2+ sparks and beyond

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2024年第5期10卷 257-258页

作者： shi-qiang wang State Key Laboratory of Membrane Biology College of Life Sciences Peking University

The calcium ion (Ca2+) is the simplest but most versatile intracellular messenger,which regulates nearly all intracellular processes,from gene expression to protein modification,from fertilization to proliferation,from cell survival to cell *** Ca2+fulfills so many functions?Why Ca2+is involved in a variety of diseases?Answering these questions relies on the microscopic understanding of intracellular Ca2+activities.

关键词：

Ca^(2+):a versatile master key for intracellular signaling cascades

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2011年第8期54卷 683-685页

作者： shi-qiang wang Michael XZHU Ernesto CARAFOLI State Key Lab of Biomembrane and Membrane Biotechnology Peking University College of Life Sciences Beijing 100871 China Department of lntegrative Biology and Pharmacology the University of Texas Health Science Center at Houston Houston 77030 USA Venetian Institute of Molecular Medicine University of Padova Padova 35121 Italy

Ca2＋is one of the most ancient and versatile intracellular messengers in both animal and plant ***2＋interacts with a huge array of signaling proteins,and coordinates the integration of non-signaling proteins into ce... 详细信息

关键词：信号蛋白主密钥细胞内蜂窝移动通信系统通用 CA 级联植物系统

β-adrenergic regulation of Ca2+ signaling in heart cells

在线全文

学校读者我要写书评

暂无评论

Biophysics Reports 2024年第5期10卷 274-282页

作者： Bo Yang shi-qiang wang Hua-Qian Yang Cyrus Tang Medical Institute Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases Soochow University State Key Lab of Membrane Biology College of Life Sciences Peking University

β-adrenergic receptors（βARs） play significant roles in regulating Ca2+signaling in cardiac myocytes,thus holding a key function in modulating heart performance.βARs regulate the influx of extracellular Ca2+and the release and uptake of Ca2+from the sarcoplasmic reticulum（SR） by activating key components such as L-type calcium channels（LTCCs）,ryanodine receptors（RyRs） and phospholamban（PLN）,mediated by the phosphorylation actions by protein kinase A（PKA）.In cardiac myocytes,the presence of β2AR provides a protective mechanism against potential overstimulation of β1AR,which may aid in the restoration of cardiac *** the Ca2+regulatory signaling pathways of βARs in cardiac myocytes and the differences among various βAR subtypes are crucial in cardiology and hold great potential for developing treatments for heart diseases.

关键词： β-adrenergic receptor (βAR) Ca2+ signaling Cardiac myocytes Compartmentalization

Joint imaging of ^(99m)TcO_4^- and ^(99m)Tc-MIBI for thyroid carcinoma recurrence and cervical metastases

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nuclear Science and Techniques 2010年第2期21卷 89-93页

作者： MAQingjie JI Tiefeng GAO shi WEN qiang wang Fan SUN Hui China-Japan Union Hospital Jilin University Changchun 130033 China

In this paper, the joint imaging of 99mTcO4- and 99mTc-methoxy isobutyl isonitrile was compared with neck Color Doppler Ultrasonography (CDU) to identify its value in thyroid carcinoma, postoperative recurrence or cervical metastases. Thyroid carcinoma patients (150 operated and 21 re-operated with suspected postoperative recurrence or cervical metastases) were retrospectively analyzed. Taking the pathological result as the gold standard, the thyroid foci (437 cases), the thyroid foci of ≥1-cm diameter (215 cases) and the suspected postoperative recurrence or cervical metastases (87 cases) were compared by diagnostic results of the joint imaging and the neck CDU. The sensitivity, specificity and accuracy of the joint imaging were 64.18%, 80.00% and 67.82%, and 44.78%, 40.00% and 43.68%, respectively, for the cases with suspected postoperative recurrence or cervical metastases. For thyroid carcinoma of ≥1-cm diameter, the sensitivity of the joint imaging was 97.75%, and the neck CDU was 89.89%, but both specificity and accuracy was similar for thyroid carcinoma independence on their diameter. For thyroid carcinoma, the sensitivity of the joint imaging was 81.99%, and the neck CDU was 89.10%. The results show that the joint imaging is sensitive for large diameter thyroid carcinoma.

关键词：甲状腺癌联合成像宫颈癌复发 MIBI 像对多普勒超声敏感性

Abnormal expression of miR-331 leads to impaired heart function

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2019年第14期64卷 1011-1017页

作者： Jin-Jing Zhang Li-Peng wang Rong-Chang Li Meng wang Zeng-Hui Huang Min Zhu Jia-Xing wang Xiu-Jie wang shi-qiang wang Ming Xu Department of Cardiology Institute of Vascular Medicine Peking University Third Hospital NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing Key Laboratory of Cardiovascular Receptors Research State Key Laboratory of Natural and Biomimetic Drugs Peking University Beijing 100191 China State Key Laboratory of Membrane Biology College of Life Sciences Peking University Beijing 100871 China Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

MicroRNAs (miRNAs) play important roles in maintaining normal heart function. Abnormal expression of miR-331 has been observed in the hearts of patients with atrial fibrillation and Marfan syndrome. However, whether miR-331 regulates cardiac function under physiological and pathological conditions still remains unknown. In the present study, we investigated the function and underlying mechanisms of miR-331 in a pressure overload-induced heart failure model and miR-331 transgenic rat model. First, we found that the expression of miR-331-3p exhibited a 1.7-fold increase in hypertrophy compared with that in the sham group (P<0.01), yet the expression of miR-331-5p remained unchanged. Furthermore, overexpression of miR-331 in cardiomyocytes and defective excitation-contraction (E-C) coupling efficiency were observed. Luciferase assays showed that miR-331-3p suppressed JPH2 expression by binding to the coding region of JPH2 mRNA. Finally, in the miR-331 transgenic rat model, JPH2 expression was suppressed at both the mRNA and protein levels in vivo, which resulted in impairment of both the E-C coupling efficiency of cardiomyocytes and systolic function of the heart. This finding mechanistically linked miR-331 to JPH2 downregulation and suggested an important role for the abnormal expression of miR-331 leading to the dysfunction of E-C coupling in heart failure.

关键词： MiR-331 Excitation-contraction coupling Junctophilin 2 Heart function

Effects of nursing care in fast-track surgery on postoperative pain, psychological state, and patient satisfaction with nursing for glioma

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2021年第20期9卷 5435-5441页

作者： Yan-Hong Deng Yi-Mei Yang Jian Ruan Lin Mu shi-qiang wang Department of Neurosurgery Chongqing University Cancer HospitalChongqing 400030China Sterile Supply Center Chongqing University Cancer HospitalChongqing 400030China Department of Anesthesiology Chongqing University Cancer HospitalChongqing 400030China

BACKGROUND The brain is the most complex organ in the human *** for a glioma always involves a multi-disciplinary *** care in fast-track surgery or enhanced recovery after surgery is such kind of work implemented by an interdisciplinary team to provide services to patients to improve their *** To explore the effects of nursing care in fast-track surgery on postoperative pain,psychological state,and patient satisfaction with nursing for *** From June 2018 to June 2020,138 patients who underwent operation for glioma at Cancer Hospital Affiliated to Chongqing University were *** were categorized into groups according to different nursing care that they *** them,69 patients receiving nursing care in fast-track surgery were included in an experimental group,and 69 patients receiving conventional postoperative nursing were included in a control *** analogue scale was used to evaluate postoperative pain in the two groups immediately after the operation and at 3 d after the ***-rating anxiety scale(SAS)and self-rating depression scale(SDS)were used to evaluate the psychological status of patients immediately after operation and on the 3rd postoperative day.A self-made satisfaction scale for patient satisfaction with nursing was used to evaluate and compare patient satisfaction with nursing between the two *** Time to excretion,time to out-of-bed activities,and length of hospital stay were significantly shorter in the observation group than in the control group(P0.05).There was no significant difference in postoperative pain score between the two groups(P>0.05).The pain score was significantly lower in the observation group than in the control group at 3 d after the operation(P<0.05).There was no significant difference in postoperative SAS or SDS score between the

关键词： Nursing care in fast-track surgery Glioma Visual analogue scale Self-rating anxiety scale Self-rating depression scale

Pollen Grain Germination and Pollen Tube Growth in Pistil of Rice

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rice science 2008年第2期15卷 125-130页

作者： CHEN shi-qiang wang Zhong LIU Man-xi XIE Zhao-wei wang Hui-hui Laboratory of Crop Inheritance Cultivation ＆ Physiology Yangzhou University Yangzhou 225009 China

The germination of pollen grain in vitro and the growth of pollen tube in the pistil of rice were observed with a microscope. The stigma was removed at different time points after pollination to study its effect on seed setting rate. The rice pollen grain started to germinate at 2 min after pollination and the pollen tube penetrated stigma into style in 5-10 min, 30 min later the end of pollen tube reached the bottom of ovary, and only some pollen tubes arrived at embryo sac at 40 min after pollination. Meanwhile, a small amount of callose began to deposit in the pollen tubes, a great deal of callose was observed at 50 min after pollination, whereas the pollen grain began to shrink. The growing rates of pollen tube in the rice stigma, style and ovary were 1500, 5000, and 5400 μm/h, respectively. The seed setting rate was quite low when the stigma was removed at about 10-15 min after pollination, gradually increased when it removed at 20 min to 50 min after pollination, and over 60% when it removed at 50 min after pollination and finally tended to be stable.

关键词： rice pollen grain germination pollen tube growth seed setting rate microscopic observation

2.3 μm nanosecond passive Q-switching of an LD-pumped Tm:YLF laser using gold nanorods as a saturable absorber

在线全文

学校读者我要写书评

暂无评论

Frontiers of Information Technology & Electronic Engineering 2021年第3期22卷 312-317页

作者： Fu-yan WU shi-qiang wang Hai-wei CHEN Hai-tao HUANG School of Physics and Electronic Engineering Jiangsu Normal UniversityXuzhou 221116China

Developing new saturable absorbers for use in the mid-infrared region has practical significance for short-pulsed lasers and related scientific and industrial *** performance of gold nanorods(GNRs)as saturable absorbers at novel mid-infrared wavelengths needs to be evaluated even though these benefit from ultrafast nonlinear responses and broadband saturable *** Q-switching of an LD-pumped 2.3μm Tm:YLF laser using GNRs was successfully realized in this *** with an 843 ns pulse width and a 6.67 kHz repetition rate were achieved using this Q-switched *** showed that GNRs provide promising passive Q-switches for 2.3μm Tm-doped lasers.

关键词： Gold nanorods Passive Q-switching 2.3μm Tm-doped laser materials

Role of FK506-binding protein in Ca^(2+) spark regulation

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2017年第19期62卷 1295-1303页

作者： Yan-Ting Zhao Yun-Bo Guo Xue-Xin Fan Hua-Qian Yang Peng Zhou Zheng Chen Qi Yuan Haihong Ye Guang-Ju Ji shi-qiang wang State Key Laboratory of Membrane Biology College of Life SciencesPeking University National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences School of Basic Medical Sciences Beijing Institute for Brain Disorders Center of SchizophreniaCapital Medical University

The elementary Ca^2＋ release events, Ca2＋ sparks, has been found for a quarter of century. However, the molecular regulation of the spark generator, the ryanodine receptor （RyR） on the sarcoplasmic reticulum, remains obscure. Although each subunit of the RyR homotetramer has a site for FKS06-binding protein （FKBP）, the role of FKBPs in modifying RyR Ca^2＋ sparks has been debated for long. One of the reasons behind the controversy is that most previous studies detect spontaneous sparks, where the mixture with out-of-focus events and local wavelets prevents an accurate characterization of Ca^2＋ sparks. In the pre- sent study, we detected Ca^2＋ sparks triggered by single L-type Ca^2＋ channels （LCCs） under loose-seal patch clamp conditions in FKS06-treated or FKBPI2.6 knockout cardiomyocytes. We found that FKBP dissociation both by FKS06 and by rapamycin decreased the Ca^2＋ spark amplitude in ventricular cardiomyocytes. This change was neither due to decreased releasable Ca^2＋ in the sarcoplasmic reticulum, nor explained by changed RyR sensitivity. Actually FKS06 increased the LCC-RyR coupling probability and curtailed the latency for an LCC to trigger a RyR Ca^2＋ spark. FKBP12.6 knockout had similar effects as FKS06/rapamycin treatment, indicating that the decreased spark amplitude was attributable to the dissociation of FKBP12.6 rather than FKBP12. We also explained how decreased amplitude of spontaneous sparks after FKBP dissociation sometimes appears to be increased or unchanged due to inappropriate data processing. Our results provided firm evidence that without the inter-RyR coordination by functional FKBP12.6, the RyR recruitment during a Ca^2＋ spark would be compromised despite the sensitization of individual RyRs.

关键词： Ca^2+ sparkFKSO6-binding protein Ryanodine receptorlntracellular calcium Excitation-contraction coupling