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Proteomic analysis and miRNA profiling of human testicular endothelial cell-derived exosomes:the potential effects on spermatogenesis

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Asian Journal of Andrology 2022年第5期24卷 478-486页

作者： Wen-Peng Song sheng-ji gu Xiao-Hui Tan Yang-Yang gu Wei-Dong Song jian-Yu Zeng Zhong-Cheng Xin Rui-Li guan Department of Urology Peking University First HospitalBeijing 100034China Institute of Urology Peking UniversityBeijing 100034China Beijing Key Laboratory of Urogenital Diseases(Male)Molecular Diagnosis and Treatment Center Beijing 100034China Department of Dental Implant Center Beijing Stomatological HospitalSchool of StomatologyCapital Medical UniversityBeijing 100050China Department of Radiation Medicine Institute of Systems BiomedicineSchool of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China

Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility,but their mechanism is *** released by various cells are recognized as cell–cell communication mediators during the initiation and progression of many ***,the current study aimed to investigate the protein and miRNA components of human testicular endothelial cell-derived exosomes(HTEC-Exos)and to explore their potential effects on *** this study,HTEC-Exos were first isolated by the ultracentrifugation method,and then identified by nanoparticle tracking analysis,transmission electron microscopy(TEM),and western *** characteristics of HTEC-Exos were examined by liquid chromatography–mass spectrometry and microRNA(miRNA)chip *** analysis was performed to explore the potential role of the exosomal content on spermatogenesis.A total of 945 proteins were identified,11 of which were closely related to spermatogenesis.A total of 2578 miRNAs were *** them,30 miRNAs demonstrated potential associations with male reproductive disorders,such as azoospermia,and spermatogenesis *** particular,11 out of these 30 miRNAs have been proven to be involved in spermatogenesis based on available *** study provides a global view of the proteins and miRNAs from HTEC-Exos,suggesting that HTEC-Exos may function as potential effectors during the process of spermatogenesis.

关键词： exosomes microRNAs proteomics testicular endothelial cells

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Comparative study of intracavernous pressure and cavernous pathology after bilateral caver nous n erve crushing and resection in rats

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Asian Journal of Andrology 2020年第6期22卷 629-635页

作者： Meng Li Yi-Ming Yuan Bi-Cheng Yang sheng-ji gu Hui-Xi Li Zhong-Cheng Xin Dong Fang Rui-Li guan Molecular Biology Laboratory of Andrology Center Peking University First HospitalPeking UniversityBeijing 100034China

This study aimed to compare the effects of bilateral cavernous nerve crushing(BCNC)and bilateral cavernous nerve resection(BCNR)on intracavernous pressure(ICP)and cavernous pathology in rats and to explore the optimal treatment time for the BCNC and BCNR ***-two male rats aged 12 weeks were randomly divided into three equal groups:Sham(both cavernous nerves exposed only),BCNC(BCN crushed for 2 min),and BCNR(5 mm of BCN resected).Erectile function was then measured at 1 week,3 weeks,and 5 weeks after nerve injury,and penile tissues were harvested for histological and molecular analyses by immunohistochemistry,immuno fluoresce nee,Western blot,and cytokine *** found that erectile function parameters including the maximum,area,and slope of ICP/mean arterial pressure(MAP)significantly decreased after BCNR and BCNC at 1 week and 3 *** 5 weeks,no significant differences were observed in ICP/MAP between the BCNC and Sham groups,whereas the ICP/MAP of the BCNR group remained significantly lower than that of the Sham *** BCNC and BCNR,the amount of neuronal-nitric oxide synthase-positive fibers,smooth muscle cells,and endothelial cells decreased,whereas the amount of collage n III con tent *** pathological cha nges recovered over time,especially in the BCNC *** fin dings demonstrate that BCNC leads to acute and reversible erectile dysfunction,thus treatme nt time should be restricted to the first 3 weeks *** contrast,the self-healing ability of the BCNR model is poor,making it more suitable for long-term treatment research.

关键词： caver nous nerve crushing cavernous nerve resection erectile dysfunction in tracavernous pressure

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Decreased levels of phosphorylated synuclein in plasma are correlated with poststroke cognitive impairment

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Neural Regeneration Research 2025年第9期20卷 2598-2610页

作者： Yi Wang Yuning Li Yakun gu Wei Ma Yuying guan Mengyuan guo Qianqian Shao Xunming ji jia Liu Department of Clinical Laboratory Beijing Bo’ai HospitalChina Rehabilitation Research CenterCapital Medical UniversityBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Science and TechnologyCollaborative Innovation Center for Brain DisordersBeijing Advanced Innovation Center for Big Data-based Precision MedicineCapital Medical UniversityBeijingChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina

Poststro ke cognitive impairment is a major secondary effect of ischemic stroke in many patients;however,few options are available for the early diagnosis and treatment of this *** aims of this study were to(1)determine the specific relationship between hypoxic andα-synuclein during the occur of poststroke cognitive impairment and(2)assess whether the serum phosphorylatedα-synuclein level can be used as a biomarker for poststro ke cognitive *** found that the phosphorylatedα-synuclein level was significantly increased and showed pathological aggregation around the cerebral infa rct area in a mouse model of ischemic *** addition,neuronalα-synuclein phosphorylation and aggregation were observed in the brain tissue of mice subjected to chronic hypoxia,suggesting that hypoxia is the underlying cause ofα-synuclein-mediated pathology in the brains of mice with ischemic *** phosphorylatedα-synuclein levels in patients with ischemic stroke were significantly lower than those in healt hy subjects,and were positively correlated with cognition levels in patients with ischemic ***,a decrease in serum high-density lipoprotein levels in stroke patie nts was significantly correlated with a decrease in phosphorylatedα-synuclein *** ischemic stroke mice did not show significant cognitive impairment or disrupted lipid metabolism 14 days after injury,some of them exhibited decreased cognitive function and reduced phosphorylatedα-synuclein *** together,our results suggest that serum phosphorylatedα-synuclein is a potential biomarker for poststroke cognitive impairment.

关键词： biomarker high-density lipoprotein ischemic stroke phosphorylatedα-synuclein poststroke cognitive impairment

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Nanomaterials-mediated lysosomal regulation:a robust protein-clearance approach for the treatment of Alzheimer’s disease

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Neural Regeneration Research 2025年第2期20卷 424-439页

作者： Mengqi Hao jianjian Chu Tinglin Zhang Tong Yin Yuankai gu Wendanqi Liang Wenbo ji jianhua Zhuang Yan Liu jie Gao You Yin Department of Neurology Second Affiliated Hospital of Naval Medical University(Shanghai Changzheng Hospital)ShanghaiChina School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghaiChina Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghaiChina Department of Clinical Pharmacy Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology Shanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina

Alzheimer’s disease is a debilitating,progressive neurodegenerative disorder characterized by the progressive accumulation of abnormal proteins,including amyloid plaques and intracellular tau tangles,primarily within the ***,crucial intracellular organelles responsible for protein degradation,play a key role in maintaining cellular *** studies have suggested a link between the dysregulation of the lysosomal system and pathogenesis of neurodegenerative diseases,including Alzheimer’s *** the normal physiological function of lysosomes hold the potential to reduce the pathological burden and improve the symptoms of Alzheimer’s ***,the efficacy of drugs in treating Alzheimer’s disease is limited,with major challenges in drug delivery efficiency and ***,nanomaterials have gained widespread use in Alzheimer’s disease drug research owing to their favorable physical and chemical *** review aims to provide a comprehensive overview of recent advances in using nanomaterials(polymeric nanomaterials,nanoemulsions,and carbon-based nanomaterials)to enhance lysosomal function in treating Alzheimer’s *** review also explores new concepts and potential therapeutic strategies for Alzheimer’s disease through the integration of nanomaterials and modulation of lysosomal *** conclusion,this review emphasizes the potential of nanomaterials in modulating lysosomal function to improve the pathological features of Alzheimer’s *** application of nanotechnology to the development of Alzheimer’s disease drugs brings new ideas and approaches for future treatment of this disease.

关键词： Alzheimer’s disease autophagy dysfunction lysosomal acidification lysosomal system nanomaterials neurodegenerative diseases

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Modeling of flow and debris ejection in blasting erosion arc machining in end milling mode

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Advances in Manufacturing 2020年第4期8卷 508-518页

作者： ji-Peng Chen Lin gu Wan-sheng Zhao Mario guagliano School of Mechanical and Electronic Engineering Nanjing Forestry UniversityNanjing 210037People's Republic of China Department of Mechanical Engineering Polytechnic University of MilanPiazza Leonardo da Vinci32MilanItaly State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240People's Republic of China

Blasting erosion arc machining(BEAM)is a typical arc discharge machining technology that was developed around 2012 to improve the machinability of difficult-to-cut *** milling BEAM has been successfully developed and preliminarily applied in ***,owing to the high complexity of the flow field and the difficulty of observing debris in the discharge gap,studies of the flow and debris in end milling BEAM are *** this study,fluid dynamics simulations and particle tracking are used to investigate the flow characteristics and debris ejection processes in end milling ***,the end milling BEAM m o d e is *** the numerical modeling parameters,geometric models,and simulation methods are presented in ***,the flow distribution and debris ejection are described,analyzed,and *** velocity and pressure distributions of the axial feed and radial feed are observed;the rotation speed and milling depth are found to have almost no effect on the flow velocity ***,debris is ejected more rapidly in the radial feed than in the axial *** particle kinetic energy tends to increase with increasing milling depth,and smaller particles are more easily expelled from the flushing *** study attempts to reveal the flow field properties and debris ejection mechanism of end milling BEAM,which will be helpful in gaining a better understanding of BEAM.

关键词： Modeling Flow Debris ejection Blasting erosion arc machining(BEAM) End milling

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Environmentally Friendly and Multifunctional Shaddock Peel-Based Carbon Aerogel for Thermal-Insulation and Microwave Absorption

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Nano-Micro Letters 2021年第7期13卷 28-41页

作者： Weihua gu jiaqi sheng Qianqian Huang Gehuan Wang jiabin Chen guangbin ji College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China Shenyang Aircraft Design Institute Yangzhou Collaborative Innovation Research Institute Co. LtdShenyang 225002People’s Republic of China

Eco-friendly electromagnetic wave absorbing materials with excellent thermal infrared stealth property,heat-insulating ability and compression resistance are highly attractive in practical *** the aforesaid requirements simultaneously is a formidable ***,ultra-light carbon aerogels were fabricated via fresh shaddock peel by facile freeze-drying method and calcination process,forming porous network *** the heating platform temperature of 70℃,the upper surface temperatures of the as-prepared carbon aerogel present a slow upward *** color of the sample surface in thermal infrared images is similar to that of the *** the maximum compressive stress of 2.435 kPa,the carbon aerogels can provide favorable *** shaddock peel-based carbon aerogels possess the minimum reflection loss value(RLmin)of−29.50 dB in X ***,the effective absorption bandwidth covers 5.80 GHz at a relatively thin thickness of only 1.7 *** the detection theta of 0°,the maximum radar cross-sectional(RCS)reduction values of 16.28 dB m^(2) can be *** simulations of RCS have aroused extensive interest owing to their ingenious design and time-saving *** work paves the way for preparing multi-functional microwave absorbers derived from biomass raw materials under the guidance of RCS simulations.

关键词： Microwave absorption Thermal insulation Carbon aerogel Radar cross-sectional simulation Multi-function

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Serendipitous discovery of warm absorbers in the Seyfert 2 galaxy IRAS 18325-5926

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Research in Astronomy and Astrophysics 2011年第10期11卷 1171-1184页

作者： Shui-Nai Zhang Qiu-sheng gu Li ji Zhi-Xin Peng Department of Astronomy Nanjing University Nanjing 210093 China Key Laboratory of Modern Astronomy and Astrophysics Nanjing University Ministry ofEducation Nanjing 210093 China Key Laboratory of Dark Matter and Space Astronomy Purple Mountain Observatory ChineseAcademy of Sciences Nanjing 210008 China

Warm absorption is a common phenomenon in Seyfert 1 s and quasars, but is rare in Seyfert 2s. We report the detection of warm absorbers with high energy resolution in the Seyfert 2 galaxy IRAS 18325-5926 for the first time with Chandra HETGS spectra. An intrinsic absorbing line system with an outflow velocity - 400 km s^-1 was found, which is contributed by two warm absorbers with FWHM of 570 km s^-1 and 1360 km s^-1, respectively. The two absorbers were adjacent, and moving transversely across our line of sight. We constrained the distance between the center and the absorbers to be a small value, suggesting that the absorbers may originate from the highly ionized accretion disk wind ejected five years ago. The perspective of this type 2 Seyfert provides the best situation in which to investigate the vertical part of the funnel-like outflows. Another weak absorbing line system with zero redshift was also detected, which could be due to Galactic absorption with very high temperature or an intrinsic outflow with a very high velocity - 6000 km s^-1.

关键词： galaxies Seyfert -- galaxies absorption lines -- X-rays galaxies -- galaxies individual IRAS 18325-5926

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Three-Dimensional Reconstruction of Welding Pool Surface by Binocular Vision

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Chinese Journal of Mechanical Engineering 2021年第3期34卷 272-284页

作者： Zunan gu ji Chen Chuansong Wu Key Laboratory for Liquid-Solid Structural Evolution&Processing of Materials Ministry of EducationInstitute of Materials JoiningShandong UniversityJinan 250061China

Current research of binocular vision systems mainly need to resolve the camera’s intrinsic parameters before the reconstruction of three-dimensional(3D)*** classical Zhang’calibration is hardly to calculate all errors caused by perspective distortion and lens ***,the image-matching algorithm of the binocular vision system still needs to be improved to accelerate the reconstruction speed of welding pool *** this paper,a preset coordinate system was utilized for camera calibration instead of Zhang’*** binocular vision system was modified to capture images of welding pool surfaces by suppressing the strong arc interference during gas metal arc *** and improving the algorithms of speeded up robust features,binary robust invariant scalable keypoints,and KAZE,the feature information of points(i.e.,RGB values,pixel coordinates)was extracted as the feature vector of the welding pool *** on the characteristics of the welding images,a mismatch-elimination algorithm was developed to increase the accuracy of image-matching *** world coordinates of matching feature points were calculated to reconstruct the 3D shape of the welding pool *** effectiveness and accuracy of the reconstruction of welding pool surfaces were verified by experimental *** research proposes the development of binocular vision algorithms that can reconstruct the surface of welding pools accurately to realize intelligent welding control systems in the future.

关键词： Surface reconstruction Welding pool Binocular imaging Gas metal arc welding Feature points matching

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From intrinsic dielectric loss to geometry patterns: Dual-principles strategy for ultrabroad band microwave absorption

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Nano Research 2021年第5期14卷 1495-1501页

作者： Bin Quan Weihua gu jiaqi sheng Xinfeng Lv Yuyi Mao Lie Liu Xiaogu Huang Zongjun Tian guangbin ji College of Materials Science and Technology Nanjing University of Aeronautics and AstronauticsNanjing210016China School of Chemistry and Materials Science Nanjing University of Information Science&TechnologyNanjing210044China National Center of Supervision and Inspection on Additive Manufacturing Products Quality Wuxi214000China Shenzhen General Test Systems Inc Taohuayuan Hi-Tech ParkShenzhen518102China College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing210016China

As electromagnetic absorbers with wide absorption bandwidth are highly pursued in the cutting-edge electronic and telecommunication industries, the traditional dielectric or magnetic bulky absorbers remain concerns of extending the effective absorption bandwidth. In this work, a dual-principle strategy has been proposed to make a better understanding of the impact of utilizing conductive absorption fillers coupled with implementing artificial structures design on the absorption performance. In the comparison based on the microscopic studies, the carbon nanotubes (CNTs)-based absorbers are confined to narrow operating bandwidth and relatively fixed response frequency range, which can not fulfill the ever-growing demands in the application. With subsequent macroscopic structure design based on the CNTs-based dielectric fillers, the artificial patterns show much more broadened absorption bandwidth, covering the majority of C-band, the whole X-band, and Ku-band, due to the tailored electromagnetic parameters and more reflections and scatterings. The results suggest that the combination of developing microscopic powder/bulky absorbers and macroscopic configuration design will fundamentally extend the effective operating bandwidth of microwave.

关键词： geometry patterns numerical simulation three-dimensional(3D)printing broadband microwave absorption

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Electrical arc contour cutting based on a compound arc breaking mechanism

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Advances in Manufacturing 2022年第4期10卷 583-595页

作者： guo-jian He Lin gu Ying-Mou Zhu ji-Peng Chen Wan-sheng Zhao K.P.Rajurkar State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai200240People’s Republic of China School of Mechanical and Electronic Engineering Nanjing Forestry UniversityNanjing210037People’s Republic of China Center for Nontraditional Manufacturing Research University of Nebraska-LincolnLincolnNEUSA

Electrical arc contour cutting(EACC)is a novel high-efficiency material cutting process that applies arc plasma to perform efficient and economical contour cutting of difficult-to-cut *** to conventional electrical arc machining(EAM),this process can remove the allowance of open structures and plates in bulk mode,rather than entirely in the form of *** with existing contour cutting methods,EACC possesses the advantages of high cutting efficiency and a deep cutting ***,a compound arc breaking mechanism(CABM),which integrates hydrodynamic force and mechanical motion,has been applied to control the discharge arc column in EACC,while also strengthening the debris expelling effect in the narrow discharge *** CABM implementation conditions were studied,based on arc column distortion images captured by a high-speed camera and simulation results of the flow field and debris distribution.A set of machining experiments was designed and conducted to optimize the performance of the proposed ***,a SiC_(p)/Al metal matrix composite(MMC)space station workpiece was machined to verify the feasibility and efficiency of this process.

关键词： Electrical arc contour cutting(EACC) Electrical arc machining(EAM) Compound arc breaking mechanism(CABM) SiC_(p)/Al metal matrix composite(MMC) High efficiency

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