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女性关键生育力评价指标雌二醇、抗苗勒管激素及窦卵泡数目的拉曼光谱检测

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第二军医大学学报 2019年第11期40卷 1223-1230页

作者：李如男郭兴道陈文杨沈丽霞高超张癸荣河北北方学院药学系河北省神经药理学重点实验室张家口075000 北京中科遗传与生殖医学研究院新技术研究室北京102629 南京医科大学第一附属医院江苏省人民医院生殖医学中心南京210029

目的选取评价女性生育力的关键指标雌二醇(E2)、抗苗勒管激素(AMH)及窦卵泡数目(AFC),探究其在健康女性和不孕女性血清样本中表面增强拉曼光谱(SERS)的差异性及其应用于临床女性生育力评价初筛的可能价值。方法收集南京医科大学第一附... 详细信息

目的选取评价女性生育力的关键指标雌二醇(E2)、抗苗勒管激素(AMH)及窦卵泡数目(AFC),探究其在健康女性和不孕女性血清样本中表面增强拉曼光谱(SERS)的差异性及其应用于临床女性生育力评价初筛的可能价值。方法收集南京医科大学第一附属医院生殖医学中心育龄期的健康女性和不孕女性血清标本共236份,所有受试者年龄均处于育龄阶段(22~49岁),平均年龄为(30.8±5.1)岁。依据临床检测结果,将血清标本分为E2高值组(>5 000 pmol/L,78例)与E2低值组(14个,68例)与AFC低值组(<7个,34例)。建立血清SERS分析法并检测各组样本的拉曼光谱,对信号进行正交偏最小二乘法判别分析(OPLS-DA)、受试者工作特征(ROC)曲线分析及置换检验等统计学处理。结果血清标本的平均拉曼光谱形态和谱峰E2高值组与低值组之间、AMH高值组与低值组之间、AFC高值组与低值组之间基本相似,但谱峰强弱在3个指标的高值组与低值组之间存在差异。在OPLS-DA模型中,E2、AMH和AFC 3个指标的高值组与低值组之间均具有明显的组间分离趋势,其ROC曲线下面积分别为0.996与0.996、0.995与0.995、1与1。结论 SERS技术具备应用于临床女性生育力初筛的潜力,血清SERS图谱作为不孕不育早期诊断的一种辅助手段值得进一步研究。

关键词：表面增强拉曼光谱不育雌二醇抗苗勒管激素窦卵泡数目正交偏最小二乘法判别分析

Tauroursodeoxycholic acid targets HSP90 to promote protein homeostasis and extends healthy lifespan

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Science China life Sciences 2025年第2期68卷 416-430页

作者： Jia-Yu liu Yao Wang Yue Guo Run-Qi Zheng Yun-Ying Wang Yan-Yan shen Yan-Hong liu Ai-Ping Cao Rui-Bo Wang Bo-Yang Xie Shuai Jiang Qiu-Ying Han Jing Chen Fang-Ting Dong Kun He Na Wang Xin Pan Tao li Tao Zhou Ai-ling li Qing xia Wei-Na Zhang Nanhu Laboratory National Center of Biomedical Analysis

As the elderly population expands, the pursuit of therapeutics to reduce morbidity and extend lifespan has become increasingly crucial. As an FDA-approved drug for chronic cholestatic liver diseases, tauroursodeoxycholic acid(TUDCA), a natural bile acid, offers additional health benefits beyond liver protection. Here, we show that TUDCA extends the lifespan and healthspan of C. elegans. Importantly, oral supplementation of TUDCA improves fitness in old mice, including clinically relevant phenotypes, exercise capacity and cognitive ***, TUDCA treatment drives broad transcriptional changes correlated with anti-aging characteristics. Mechanistically, we discover that TUDCA targets the chaperone HSP90 to promote its protein refolding activity. This collaboration further alleviates aging-induced endoplasmic reticulum(ER) stress and facilitates protein homeostasis, thus offering resistance to aging. In summary, our findings uncover new molecular links between an endogenous metabolite and protein homeostasis, and propose a novel anti-aging strategy that could improve both lifespan and healthspan.

关键词： tauroursodeoxycholic acid lifespan healthspan protein homeostasis

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低剂量阿司匹林预防子痫前期的关注焦点

四川大学学报（医学版） 2023年第2期54卷 450-454页

作者：王晶沈丽霞王子莲潘昭颐中山大学附属第一医院妇产科广州510080 香港中文大学妇产科系中国香港999077

孕期服用低剂量阿司匹林是目前国内外专家广泛推荐的子痫前期(preeclampsia, PE)预防方法。尽管该方法在PE预防中已有较多报道,但是阿司匹林标准化预防PE的治疗方案仍未在国内的临床指南中达成共识。这是由于在现有研究中,高危人群服用... 详细信息

孕期服用低剂量阿司匹林是目前国内外专家广泛推荐的子痫前期(preeclampsia, PE)预防方法。尽管该方法在PE预防中已有较多报道,但是阿司匹林标准化预防PE的治疗方案仍未在国内的临床指南中达成共识。这是由于在现有研究中,高危人群服用阿司匹林的剂量、初始时间和筛查方法不同,尚未对阿司匹林用药方案形成统一结论。本文基于循证医学证据,总结现有指南推荐意见,对阿司匹林在预防PE时的剂量、具体用药时机、起止时间等焦点问题进行综述,为临床实践提供依据。综合目前阿司匹林预防PE的研究结果及临床实践,我们建议:PE风险筛查应在妊娠11~13+6周时进行;PE高风险孕妇预防性使用阿司匹林的推荐剂量为150 mg/d,最低有效推荐剂量为100 mg/d;PE高风险孕妇于妊娠16周前开始口服低剂量阿司匹林;妊娠36周为低剂量阿司匹林的停药时机。

关键词：子痫前期阿司匹林预防低剂量用药

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槲皮素固体脂质纳米粒对Aβ25-35损伤的PC12细胞雌激素样神经保护作用的研究

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中国药理学通报 2020年第10期36卷 1409-1415页

作者：郭童林甄艳杰赵雨薇沈丽霞河北北方学院药学系河北省神经药理学重点实验室河北张家口075000

目的考察槲皮素固体脂质纳米粒(quercetin solid lipid nanoparticles,Que-SLN)对Aβ25-35损伤的PC12细胞的雌激素样神经保护作用。方法通过熔融超声法制备Que-SLN并进行理化性质评价;Transwell小室考察Que-SLN体外血脑屏障通透性;MTT... 详细信息

目的考察槲皮素固体脂质纳米粒(quercetin solid lipid nanoparticles,Que-SLN)对Aβ25-35损伤的PC12细胞的雌激素样神经保护作用。方法通过熔融超声法制备Que-SLN并进行理化性质评价;Transwell小室考察Que-SLN体外血脑屏障通透性;MTT法测定不同处理组细胞活力、免疫荧光染色法和Western blot技术检测ERα和ERβ蛋白的表达。结果制备的Que-SLN包封率为(90.54±1.36)%,载药量(4.33±0.06)%,粒径(191.23±3.25)nm,电位(-24.40±1.24)mV,外观呈球形,形态较圆整,大小均一;与Que组相比,Que-SLN的血脑屏障通透性增加(P<0.01);Que-SLN(50、100、200μmol·L^-1)浓度组的细胞的存活率分别为(116.79±2.75)%(P<0.01),(119.46±1.89)%(P<0.01),(107.55±3.17)%(P<0.05),与Aβ25-35组相比,Que-SLN各浓度组均能增加细胞的存活率;免疫荧光染色和Western blot结果显示,与Aβ25-35组相比,Que-SLN能提高ERα蛋白表达量,对ERβ蛋白表达无影响。结论熔融超声法适用于Que-SLN的制备,制备成Que-SLN后增加了Que的血脑屏障通透性,Que-SLN对Aβ25-35诱导的PC12细胞的损伤具有雌激素样神经保护作用。

关键词：槲皮素固体脂质纳米粒血脑屏障雌激素样作用 ERα ERβ

Palindrome-mediated DNA nanotubes with cell-specific aptamers to improve targeted antitumor effects and reduce toxicity on non-small cell lung cancer

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Science China life Sciences 2025年第2期68卷 454-466页

作者： Cheng Zheng Lanlan Song Chang Yu lingye Zhu Jing Zhang Ning Wang Mengchu liu Shini li liangxing Wang Zhifa shen xiaoying Huang Division of Pulmonary Medicine the First Affiliated Hospital of Wenzhou Medical UniversityKey Laboratory of Interdiscipline and Translational Medicine Key Laboratory of Laboratory Medicine Ministry of Education of Chinaand Zhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life SciencesWenzhou Medical University Intervention Department the First Affiliated Hospital of Wenzhou Medical University

Chemotherapy is regarded as a widely used and effective treatment strategy for lung cancer, although most conventional chemotherapeutics cause severe toxic side-effects due to their indiscriminate attacks on both cancerous and normal cells. Although nucleic acid nanomaterials are emerging as a promising drug delivery strategy, their clinical applications are limited by rapid degradation by nucleases and difficulties in targeting cancer cells. In this study, we have developed a Rhein-loaded aptamer-based DNA nanotube(DNT-S6@Rhein) for the targeted and efficient therapy of non-small cell lung cancer. Through the palindrome segments, two specified oligonucleotides were hybridized and folded into the well-defined nanotubes(DNT-S6), with the S6 aptamer distributed outside. The obtained nanotubes exhibited excellent serum stability and targeting ability towards A549 cells due to the firm structure and decoration of the S6 aptamer. Rhein, as an antitumor drug and DNA intercalator, can be effectively inserted into the DNT-S6. The drug-loaded nanotubes rapidly disassembled in intracellular environment and then the released Rhein was found to activate cellular apoptotic process and significantly suppress proliferation, migration and invasion of A549 cells. Moreover, DNT-S6@Rhein could efficiently accumulate in tumor regions, offering compelling therapeutic efficacy and biocompatibility under both in vitro and in vivo settings. These findings of this study provide a promising strategy for mitigating the inevitable systemic side-effects of chemotherapy and expand the potential application of DNA nanostructure on targeted drug delivery.

关键词： DNA nanotube self-assembly Rhein targeted drug delivery cancer therapy

Interleukin-17A facilitates tumor progression via upregulating programmed death ligand-1 expression in hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 176-198页

作者： Zhong-xia Yang li-Ting Zhang xiao-Jun liu Xue-Bin Peng xiao-Rong Mao The First School of Clinical Medicine Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of Infectious Diseases The First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of Radiotherapy Gansu Provincial HospitalLanzhou 730000Gansu ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)is an inflammation-associated tumor with a dismal *** has become an important treatment strategy for HCC,as immunity is closely related to inflammation in the tumor *** regulates the expression of programmed death ligand-1(PD-L1)in the immunosuppressive tumor microenvironment and affects im-munotherapy ***-17A(IL-17A)is involved in the remodeling of the tumor microenvironment and plays a protumor or antitumor role in different *** hypothesized that IL-17A participates in tumor progression by affe-cting the level of immune checkpoint molecules in *** upregulation of PD-L1 expression in HCC cells by IL-17A was assessed by reverse transcription PCR,western blotting,and flow *** studies were conducted with gene knockout models and pathway *** function of IL-17A in immune evasion was explored through coculture of T cells and HCC *** effects of IL-17A on the malignant biological behaviors of HCC cells were evaluated in vitro,and the antitumor effects of an IL-17A inhibitor and its synergistic effects with a PD-L1 inhibitor were studied in *** IL-17A upregulated PD-L1 expression in HCC cells in a dose-dependent manner,whereas IL-17A receptor knockout or treatment with a small mothers against decapentaplegic 2 inhibitor diminished the PD-L1 expression induced by ***-17A enhanced the survival of HCC cells in the coculture ***-17A increased the viability,G2/M ratio,and migration of HCC cells and decreased the apoptotic *** D1,VEGF,MMP9,and Bcl-1 expression increased after IL-17A treatment,whereas BAX expression *** combination of IL-17A and PD-L1 inhibitors showed synergistic antitumor efficacy and increased cluster of differentiation 8+T lymphocyte infiltration in an HCC mouse *** IL-17A upregulates PD-L1 expression via the IL-17A receptor/phosphorylation-small mothers against

关键词： Interleukin-17A Programmed death ligand-1 Interleukin-17A receptor Small mothers against decapentaplegic 2 Hepatocellular carcinoma Immunotherapy

氮肥类型与施用量对夏玉米生长发育及氮肥利用的影响

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华北农学报 2006年第1期21卷 115-120页

作者：易镇邪王璞张红芳申丽霞中国农业大学农学与生物技术学院北京100094

以减少氮肥损失、提高夏玉米氮肥利用率为着眼点，对3种类型氮肥（普通尿素、包膜尿素、复合肥）不同施用量对夏播玉米品种郑单958与农大108的生长发育及氮肥利用的影响进行了研究。结果表明，在本试验施氮范围内（折合N0～180kg／hm^2... 详细信息

以减少氮肥损失、提高夏玉米氮肥利用率为着眼点，对3种类型氮肥（普通尿素、包膜尿素、复合肥）不同施用量对夏播玉米品种郑单958与农大108的生长发育及氮肥利用的影响进行了研究。结果表明，在本试验施氮范围内（折合N0～180kg／hm^2），两品种随施氮量增加而显著增产；同一施氮水平下以复合肥增产效应最好，但3种类型氮肥之间的产量差异不显著。两品种穗粒数随施氮量增加而增加，与氮肥类型关系不大，而千粒重受氮肥类型影响较大，且品种间表现有差异。LAI、干物重与叶片叶绿索含量均随施氮量增大而增大，且复合肥和包膜尿素对以上3个性状值的提高较普通尿素更明显。与普通尿素相比，包膜尿素与复合肥的氮肥利用率较高，但不能相应地提高氮收获指数。各类型氮肥的利用率均具有基因型差异。

关键词：夏玉米氮肥类型施氮量生长发育氮肥利用率

Reversible phase transition poly(benzyl methacrylate)/ionic liquid electrolytes for effective overheating protection in lithium batteries

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Journal of Energy Chemistry 2025年第2期101卷 76-86页

作者： Qian Yu Wei Sun Jialing Zhu Haoxiang li Shuai Wang Longqing Huang Qian Qiu Haoran Tian He Miao Fu Wang Chunfei Zhang Jinliang Yuan Lan xia Ningbo Innovation Team on New Energies and Marine Applications Faculty of Maritime and Transportation Ningbo University Technical University of Munich TUM School of Natural Sciences Department of Physics Chair for Functional Materials

Battery safety is influenced by various factors,with thermal runaway being one of the most significant *** most studies have concentrated on developing one-time,self-activating mechanism for thermal protection,such as temperature-responsive electrodes,and thermal-shutdown separators,these methods only provide irreversible ***,reversible temperature-sensitive electrolytes have emerged as promising alternatives,offering both thermo-reversibility and self-protective ***,further research is crucial to fully understand these thermal-shutdown *** this study,we propose lower critical solution temperature(LCST) phase behavior poly(benzyl methacrylate)/imidazolium-based ionic liquid mixtures to prepare temperature-sensitive electrolytes that provide reversible thermal shutdown protection of *** electrolyte features an appropriate protection temperature(～105℃) and responds quickly within a 1 min at 105℃,causing cells to hardly discharge as the voltage suddenly drops to 3.38 V,and providing efficient thermal shutdown protection within 30 *** cooling back to room temperature,the battery regains its original ***,the electrolyte exhibits excellent cycling stability with the capacity retention of the battery is 91.6% after 500 *** work provides a viable solution for preventing batteries from thermal runaway triggered by overheating.

关键词： Ionic liquid lithium-ion battery Lower critical solution temperature Overheating Safety Thermal runaway

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施氮对夏玉米碳氮代谢及穗粒形成的影响

植物营养与肥料学报 2007年第6期13卷 1074-1079页

作者：申丽霞王璞兰林旺孙西欢太原理工大学水利科学与工程学院山西太原030024 中国农业大学农学与生物技术学院北京100094

以夏玉米杂交种郑单958为材料,对不同施氮水平下玉米产量、产量构成、粒数形成关键期植株体的碳氮代谢及碳氮代谢的关键酶进行了研究。结果表明,氮肥对玉米产量的影响主要体现在对穗粒数、穗粒重的影响上。施氮量为180 kg/hm2时,显著促... 详细信息

以夏玉米杂交种郑单958为材料,对不同施氮水平下玉米产量、产量构成、粒数形成关键期植株体的碳氮代谢及碳氮代谢的关键酶进行了研究。结果表明,氮肥对玉米产量的影响主要体现在对穗粒数、穗粒重的影响上。施氮量为180 kg/hm2时,显著促进玉米穗粒数、穗粒重的增加;施氮量增加至240 kg/hm2时,促进作用下降。施氮明显促进大喇叭口期至灌浆期植株体的碳氮代谢,使碳氮代谢的关键酶硝酸还原酶(NR)、谷氨酰胺合成酶(GS)和蔗糖磷酸合成酶(SPS)活性提高,增强光合产物的积累和运输,从而满足生殖生长的需求,促进穗粒数的形成,提高产量。在抽丝前供氮充足的前提下,抽丝期施氮对增产意义不大。

关键词：玉米氮肥碳氮代谢产量穗粒数