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Acute myocardial necrosis caused by aconitine poisoning:A case report

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World Journal of Clinical Cases 2022年第33期10卷 12416-12421页

作者： Yu-Ping Liao Li-han shen Li-Hua Cai Jie Chen han-Quan Shao Department of Critical Care Medicine Dongguan People’s HospitalDongguan 523058Guangdong ProvinceChina

BACKGROUND Herbal medicine has a long history of use in the prevention and treatment of disease and is becoming increasingly popular ***,there are also widespread concerns about its *** them,the cardiotoxicity of aconitine has been *** SUMMARY We report a case of a 61-year-old male with aconitine poisoning presenting with malignant arrhythmia and severe cardiogenic shock,which was successfully managed with aggressive advanced life support and heart *** This is the first case wherein in vivo cardiac pathology was obtained,confirming that aconitine caused acute myocardial necrosis.

关键词： Aconitine ECMO Myocardial necrosis Ventricular arrhythmia Herbal medicine Case report

Quantitative Electron Tomography for Accurate Measurement of Precipitates Microstructure Parameters in Al–Cu–Li Alloys

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Acta Metallurgica Sinica(English Letters) 2022年第11期35卷 1882-1894页

作者： Shi-Yong Li Ruo-han shen Yu-Tao He Cui-Lan Wu Jiang-Hua Chen Centre for High Resolution Electron Microscopy College of Materials Science and EngineeringHunan UniversityChangsha 410082China College of Materials Science and Engineering Hainan UniversityHaikou 570228China

Mechanical theories show that properties of alloys are strongly dependent on the morphological parameters oftheir strengthening ***,accurate measurement of precipitates microstructure parameters is still a challenging *** this article,we develop a quantitative electron tomography method by combining computer vision technology to accurately characterize the three-dimensional microstructure parameters,such as volume fractions,sizes and distributions,of the T_(1) and δ’/θ’/δ’ precipitates in Al-Cu-Li(-Mg) *** they have extremely large aspect-ratios in shape and large numbers in density upon formation in the Al matrix,these thin plate-like precipitates are difficult to be characterized quantitatively without the assistance of computer vision *** is shown that the property difference between two peak-aged states of the alloy can be well explained with the quantitative precipitate parameters correctly *** these correct precipitate data,we also tested the validity of current mechanical models for projecting the contribution of precipitates to the strengths of the alloy,demonstrating that quantitative relations between strength and micro structure parameters still need to be refined.

关键词： Al–Cu–Li–Mg alloy T1 precipitate δ′/θ′/δ′composite precipitate Morphological parameters Electron tomography Computer vision

Comparison and integration of final electromagnetic stirring and thermal soft reduction on continuous casting billet

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Journal of Iron and Steel Research International 2021年第2期28卷 160-167页

作者： Yan-shen han Wei Yan Jiang-shan Zhang Jun Chen Wei-qing Chen Qing Liu State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China Xiangtan Iron&Steel Co. Ltd.of Hunan ValinXiangtan 411101HunanChina

Final electromagnetic stirring(F-EMS)and thermal soft reduction(TSR)are techniques that improve the inner quality of continuous casting billets,but they have rarely been applied *** application effects of F-EMS and TSR were compared,and a process integrating F-EMS and TSR was adopted for a billet continuous caster.A heat transfer model was established to calculate the thermal behavior of 82A tire cord steel *** locations of F-EMS and TSR were determined,followed by conducting a series of plant trials,involving F-EMS alone,TSR alone,and the integrated process of F-EMS and *** results showed that F-EMS or TSR could effectively improve the inner quality of the billet under their respective suitable working ***,F-EMS was found to be more helpful in terms of improving central segregation,while TSR tended to improve V-segregation,central porosity,and *** integration of F-EMS and TSR allowed the advantages of each technique to be utilized,thereby better improving the inner *** all the working conditions,82A steel billet showed optimum inner quality when the current of F-EMS was 240 A and the cooling intensity of TSR was 2.2 m^(3) h^(−1).These findings demonstrate that the integration of F-EMS and TSR is promising for application on continuous casting billets.

关键词： Billet continuous casting Final electromagnetic stirring Thermal soft reduction Central segregation Inner quality

Role of microRNA-25 in high glucose cultured Müller glia

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International Journal of Ophthalmology(English edition) 2021年第5期14卷 643-648页

作者： Yu Liu han shen Song-Tao Yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina

AIM:To investigate the role of microRNA-25(miR-25)in proliferation and apoptosis of retinal Müller glia(MG)under high glucose ***:The purity of the cultured cells was verified by immunocytochemistry and flow cytometry using antibodies that specifically recognized *** expression level of miR-25 under normal and high glucose conditions were validated by quantitative reverse transcription polymerase chain reaction(RT-q PCR).miR-25 mimics and negative control were transfected into MG and multiple functional experiments including cell counting kit-8 assay,EDU assay,and flow cytometry were conducted to explore the effects of miR-25 on the proliferation and apoptosis of high glucose cultured MG(HGMG).RESULTS:Immunocytochemistry and flow cytometry confirmed the high purity of primary cultured *** results showed that the expression level of miR-25 was significantly repressed in HGMG,while overexpression of miR-25 by miR-25 mimic markedly inhibited the high glucose induced cell apoptosis and promoted the proliferation of ***:The expression level of miR-25 is significantly downregulated in HGMG and its overexpression could attenuate the high glucose damages on MG by promoting proliferation and reducing apoptosis.

关键词： microRNA-25 Müller glia proliferation apoptosis

PRMT5和CDKN2B在宫颈癌组织的表达及临床意义

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疑难病杂志 2024年第4期23卷 412-417页

作者：胡晓菡周强孙武陈静沈瀚李强南京大学医学院附属鼓楼医院检验科 210008 南京大学医学院附属鼓楼医院病理科 210008 南京大学医学院附属鼓楼医院肿瘤科 210008 南京大学医学院附属鼓楼医院妇产科 210008

目的研究蛋白精氨酸甲基转移酶5(PRMT5)、细胞周期蛋白依赖性激酶抑制剂2B(CDKN2B)在宫颈癌中的表达及临床意义。方法收集2019年3月—2020年3月南京大学医学院附属鼓楼医院妇产科诊治宫颈癌患者88例。免疫组织化学法检测宫颈癌和癌旁组... 详细信息

目的研究蛋白精氨酸甲基转移酶5(PRMT5)、细胞周期蛋白依赖性激酶抑制剂2B(CDKN2B)在宫颈癌中的表达及临床意义。方法收集2019年3月—2020年3月南京大学医学院附属鼓楼医院妇产科诊治宫颈癌患者88例。免疫组织化学法检测宫颈癌和癌旁组织中PRMT5、CDKN2B表达;采用Spearman相关分析PRMT5与CDKN2B表达的相关性;比较不同临床特征宫颈癌癌组织中PRMT5、CDKN2B表达的差异;Kaplan-Meier曲线评估PRMT5、CDKN2B表达对宫颈癌患者无进展生存预后的影响;多因素Cox回归分析宫颈癌患者无进展生存预后的影响因素。结果癌组织中PRMT5蛋白阳性率70.45%(62/88),高于癌旁组织6.82%(6/88)(χ^(2)=75.155,P<0.001)。宫颈癌组织中CDKN2B阳性率22.73%(20/88),低于癌旁组织79.55%(71/88)(χ^(2)=75.336,P<0.001)。宫颈癌中PRMT5与CDKN2B呈负相关(r=-0.734,P<0.001)。FIGOⅠB2~ⅡA期、有淋巴结转移宫颈癌组织中PRMT5阳性率高于FIGOⅠA~ⅠB1期、无淋巴结转移者,而CDKN2B阳性率则降低(χ^(2)/P=6.359/0.012、4.606/0.032、5.205/0.023、3.893/0.048)。PRMT5阳性组3年累积无进展生存率74.19%(46/62),低于PRMT5阴性组92.31%(24/26)(Log-Rankχ^(2)=4.386,P=0.017)。CDKN2B阴性组3年累积无进展生存率75.00%(51/68),低于CDKN2B阳性组95.00%(19/20)(Log-Rankχ^(2)=4.423,P=0.012)。FIGO分期ⅠB2~ⅡA期、合并淋巴结转移、PRMT5阳性、CDKN2B阴性是影响宫颈癌患者无进展生存预后的独立危险因素[OR(95%CI)=1.407(1.159~1.696),1.464(1.201~1.784),1.614(1.189~2.192),1.595(1.191~2.136)]。结论宫颈癌组织中PRMT5表达升高,CDKN2B表达降低,两者与宫颈癌患者的不良临床病理特征有关,是评估宫颈癌预后的标志物。

关键词：宫颈癌蛋白精氨酸甲基转移酶5 细胞周期蛋白依赖性激酶抑制剂2B 预后肿瘤标志物

南京地区1122例肺炎患者肺炎支原体感染流行特点及IL-6、PCT检测的诊断价值

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临床检验杂志 2024年第8期42卷 624-627页

作者：谢安然董智慧许红攀沈瀚南京大学医学院附属鼓楼医院检验科南京210008

目的分析肺炎支原体(MP)感染的特点及流行趋势,探讨白细胞介素-6(IL-6)和降钙素原(PCT)检测对MP感染的应用价值。方法回顾性分析本院2022年9月1日至2023年8月31日期间1122例肺炎患者的MP核酸检测结果及患者年龄、性别、采样日期和就诊... 详细信息

目的分析肺炎支原体(MP)感染的特点及流行趋势,探讨白细胞介素-6(IL-6)和降钙素原(PCT)检测对MP感染的应用价值。方法回顾性分析本院2022年9月1日至2023年8月31日期间1122例肺炎患者的MP核酸检测结果及患者年龄、性别、采样日期和就诊类别等临床资料,分析MP核酸阳性检出率在不同性别、年龄、就诊类别和季节的差异。绘制受试者工作特征(ROC)曲线,分析IL-6、PCT水平及二者比值在肺炎支原体肺炎(MPP)中的诊断价值。结果1122例肺炎患者中,MP总阳性检出率为24.96%。男性和女性患者的MP核酸阳性检出率分别为23.28%和27.29%,差异无统计学意义(P>0.05)。MP核酸阴性患者的中位年龄为37岁,阳性患者的中位年龄为7岁,差异有统计学意义(P<0.01)。MP核酸阳性患者主要集中于1~2岁(20.37%)、3~5岁(25.60%)和6~8岁(67.40%)。913例住院患者和209例门诊患者的MP核酸阳性检出率分别为22.45%和35.89%,差异有统计学意义(P<0.01)。秋季的MP核酸阳性检出率最高(42.47%)。MP可与其他5种呼吸道病原体发生混合感染,其中与人鼻病毒混合感染的患者数最多(17例)。ROC曲线分析显示,对全年龄段患者而言,IL-6/PCT比值诊断MP感染的ROC曲线下面积(AUCROC)为0.746,优于IL-6和PCT单独检测;对6~8岁的MP易感患者而言,IL-6诊断MP感染的AUCROC为0.767,预测价值最高。结论MPP易在秋季流行,6~8岁儿童是主要的MP易感群体。IL-6/PCT比值有望成为MPP的辅助诊断和预后评估指标,IL-6水平对MP易感人群感染MP的预测价值最高。

关键词：肺炎支原体流行趋势白细胞介素-6 降钙素原

多黏菌素耐药基因在全球肺炎克雷伯菌中的流行分布特点

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临床检验杂志 2024年第7期42卷 548-552页

作者：翟俊斌曹小利沈瀚陈雨欣南京医科大学鼓楼临床医学院南京210000

目的探讨多黏菌素耐药基因(mobile colistin resistance,mcr)在肺炎克雷伯菌中的流行分布特点。方法使用Aspera软件从NCBI基因组数据库下载肺炎克雷伯菌的基因组序列,采用CheckM v1.1.3和Quest 5.0.2软件进行质量过滤,使用Prokka v1.13... 详细信息

目的探讨多黏菌素耐药基因(mobile colistin resistance,mcr)在肺炎克雷伯菌中的流行分布特点。方法使用Aspera软件从NCBI基因组数据库下载肺炎克雷伯菌的基因组序列,采用CheckM v1.1.3和Quest 5.0.2软件进行质量过滤,使用Prokka v1.13对基因组进行注释。从NCBI网站下载所有mcr基因序列,采用makeblastdb命令构建数据库,再采用自编写的Perl脚本程序从注释文件提取所有基因核苷酸序列为Query,进行本地BLASTN分析,获得mcr阳性的菌株。从PubMLST网站下载肺炎克雷伯菌7个管家基因的基因序列文件和Profiles文件为数据库,采用自编写的Perl脚本程序提取基因核苷酸序列为Query,进行本地BLASTN分析,确定每个基因组的序列类型(ST)。使用自编写的perl程序从下载的肺炎克雷伯菌基因组GenBank文件中批量提取菌株元信息(如分离源、样本类型、国家和日期等)以分析mcr阳性菌株的分布特点。使用卡方检验对比不同ST型之间mcr分布的差异。结果在本研究纳入分析的全球11429个肺炎克雷伯菌基因组中,207株细菌中检出229个mcr。mcr变异体共有6种,以mcr-1(87/229,38.0%)、mcr-8(59/229,25.8%)和mcr-9(59/229,25.8%)为主;207株细菌检出76种ST型,以ST15(21/207,10.1%)、ST43(17/207,8.2%)、ST11(16/207,7.7%)和ST147(16/207,7.7%)为主。87株mcr-1阳性菌株有31种ST,以ST43(17/87,19.5%)和ST15(10/87,11.5%)为主;59株mcr-8菌株有17种ST,以ST43(17/59,28.8%)和ST11(9/59,15.3%)为主;59株mcr-9菌株有27种ST,以ST147(11/59,18.6%)和ST274(11/59,18.6%)为主。不同的ST型携带的mcr变异体之间的差异亦有统计学意义。mcr阳性肺炎克雷伯菌来自全球五大洲的28个国家,以中国(68/207,32.9%)和泰国(45/207,21.7%)为主,主要来源于人体(100/207,48.3%),流行时间集中于2015年至2018年。结论在全球肺炎克雷伯菌中,mcr的流行以mcr-1、mcr-8、mcr-9为主。mcr-1的流行以ST15和ST43为主,mcr-8的流行以ST11和ST43为主,mcr-9的流行以ST147和ST274为主。加强该类细菌的监测对于预防院内感染控制具有重要作用。

关键词：肺炎克雷伯菌多黏菌素耐药基因序列分型基因组

Mechanism of interactions betweenα-conotoxin RegIIA and carbohydrates at the humanα3β4 nicotinic acetylcholine receptor

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Marine Life Science & Technology 2022年第1期4卷 98-105页

作者： Meiling Zheng han-shen Tae Liang Xue Tao Jiang Rilei Yu Molecular Synthesis Center&Key Laboratory of Marine Drugs Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao266003China Innovation Platform of Marine Drug Screening&Evaluation Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266100China Illawarra Health and Medical Research Institute(IHMRI) University of WollongongWollongongNSW2522Australia Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao266003China

Conotoxins are marine peptide toxins from marine cone ***α-conotoxin RegIIA can selectively act on human(h)α3β4 nicotinic acetylcholine receptor(nAChR),and is an important lead for drug *** high-resolution cryo-electron microscopy structure of theα3β4 nAChR demonstrates several carbohydrates are located near the orthosteric binding sites,which may affectα-conotoxin *** chains can modify the physical and chemical properties of proteins by changing the conformation,hydrophobicity,quality and size of the *** purpose of this study is to explore the effect of oligosaccharide chains on the binding modes and activities of RegIIA and its derivatives at hα3β4 *** computational simulations,we designed and synthesized RegIIA mutants at position 14 to explore the importance of residue H14 to the activity of the *** dynamics simulations suggest that the oligosaccharide chains affect the binding of RegIIA at the hα3β4 nAChR through direct interactions with H14 and by affecting the C-loop conformation of the binding *** studies on H14 analogues suggest that in addition to forming direct interactions with the carbohydrates,the residue might play an important role in maintaining the conformation of the ***,this study further clarifies the structure–activity relationship ofα-conotoxin RegIIA at the hα3β4 nAChR and,also provides important experimental and theoretical basis for the development of new peptide drugs.

关键词： Oligosaccharide chains nAChR Conotoxin Action mechanism

Bowel inflammatory presentations on computed tomography in adult patients with severe aplastic anemia during flared inflammatory episodes

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World Journal of Clinical Cases 2023年第3期11卷 576-597页

作者： Xi-Chen Zhao Cheng-Jiang Xue Hui Song Bin-han Gao Fu-shen han Shu-Xin Xiao Department of Hematology The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina Department of Neurosurgery The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina Department of Radiology The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina Department of Pneumology The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

BACKGROUND Patients with severe aplastic anemia(SAA)frequently present with inflammatory episodes,and during flared inflammatory episodes,hematopoietic function is further *** gastrointestinal tract is the most common site for infectious and inflammatory diseases,and its structural and functional features confer on it the most potent capacity to affect hematopoietic and immune *** tomography(CT)is a readily accessible approach to provide highly useful information in detecting morphological changes and guiding further *** To explore CT imaging presentations of gut inflammatory damage in adult SAA patients during inflammatory *** We retrospectively evaluated the abdominal CT imaging presentations of 17hospitalized adult patients with SAA in search of the inflammatory niche when they presented with systemic inflammatory stress and exacerbated hematopoietic *** this descriptive manuscript,the characteristic images that suggested the presence of gastrointestinal inflammatory damage and related imaging presentations of individual patients were enumerated,analyzed and *** All eligible patients with SAA had CT imaging abnormalities that suggested the presence of an impaired intestinal barrier and increased epithelial *** inflammatory damages were concurrently present in the small intestine,the ileocecal region and the large *** readily identified imaging signs,such as bowel wall thickening with mural stratification(“water holo sign”,“fat holo sign”,intramural gas and subserosal pneumatosis)and mesenteric fat proliferation(fat stranding and“creeping fat sign”),fibrotic bowel wall thickening,“balloon sign”,rugged colonic configuration,heterogeneity in the bowel wall texture,and adhered and clustered small bowel loop(including various patterns of“abdominal cocoon”),occurred at a high incidence,which suggested that the damaged gastrointestinal tract is a common inflammatory niche res

关键词： Aplastic anemia Computed tomography Bowel inflammatory damage Fat holo sign Balloon sign Abdominal cocoon