检索结果-南通市图书馆

Conditional disruption of the osterix gene in chondrocytes during early postnatal growth impairs secondary ossification in the mouse tibial epiphysis

引用

Bone Research 2019年第3期7卷 310-317页

作者： Weirong Xing Catrina Godwin sheila pourteymoor Subburaman Mohan 1Musculoskeletal Disease Center VA Loma Linda Healthcare System Loma Linda CA 92357 USA Department of Medicine Loma Linda University Loma Linda CA 92357 USA Department of Orthopedics Loma Linda University Loma Linda CA 92357 USA Department of Biochemistry Loma Linda University Loma Linda CA 92357 USA

In our previous studies, we have found that the prepubertal increase in thyroid hormone levels induces osterix(Osx) signaling in hypertrophic chondrocytes to transdifferentiate them into osteoblasts. To test if Osx expressed in chondrocytes directly contributes to transdifferentiation and secondary ossification, we generated Osx^flox/flox;Col2-Cre-ERT2 mice and knocked out Osx with a single injection of tamoxifen at postnatal day(P) 3 prior to evaluation of the epiphyseal bone phenotype by μCT, histology, and immunohistochemistry(IHC) at P21. Vehicle(oil)-treated Osx^flox/flox;Col2-Cre-ERT2 and tamoxifen-treated, Cre-negative Osx^flox/flox mice were used as controls.μCT analysis of tibial epiphyses revealed that trabecular bone mass was reduced by 23% in the Osx conditional knockout(c KO) compared with control mice. Trabecular number and thickness were reduced by 28% and 8%,respectively, while trabecular separation was increased by 24% in the c KO mice. Trichrome staining of longitudinal sections of tibial epiphyses showed that bone area and bone area adjusted for total area were decreased by 22% and 18%, respectively. IHC studies revealed the presence of abundant Osx-expressing prehypertrophic chondrocytes in the epiphyses of control mice at P10, but not in the cKO mice. Furthermore, expression levels of MMP13, COL10, ALP, and BSP were considerably reduced in the epiphyses of cKO mice. We also found that Osx overexpression in ATDC5 chondrocytes increased expression of Col10, Mmp13, Alp, and Bsp. Our data indicate that Osx expressed in chondrocytes plays a significant role in secondary ossification by regulating expression of genes involved in chondrocyte hypertrophy and osteoblast transdifferentiation.

关键词： ossification thyroid hormone Large chondrocyte

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metabolic disease and the liver: A review

引用

World Journal of Hepatology 2024年第1期16卷 33-40页

作者： Márcia Vargas sheila de Castro Cardoso Toniasso Patricia G Riedel Camila Pereira Baldin Francielle Lopes dos Reis Robson Martins Pereira Maria Carlota Borba Brum Dvora Joveleviths Mario Reis Alvares-da-Silva Program of Graduate Science in Gastroenterology and Hepatology Federal University of Rio Grande do SulPorto Alegre 90040-060Rio Grande do SulBrazil School of Medicine Federal University of Rio Grande do SulPorto Alegre 90040-060Brazil Program of Graduate Science in Gastroenterology and Hepatology Clinicas Hospital of Porto AlegrePorto Alegre 90410000Brazil Medicine Faculty Federal University of Rio Grande do Sul Federal University of Rio Grande do SulPorto Alegre 90040-060Brazil Division of Gastroenterology and Hepatology Clinicas Hospital of Porto AlegrePorto Alegre 90035007Brazil

Metabolic dysfunction-associated steatotic liver disease(MASLD)is the most common liver disease worldwide,with an estimated prevalence of 31%in Latin *** presence of metabolic comorbidities coexisting with liver disease varies substantially among *** is acknowledged that obesity is boosting the type 2 diabetes mellitus“epidemic,”and both conditions are significant contributors to the increasing number of patients with *** steatohepatitis represents a condition of chronic liver inflammation and is considered the most severe form of *** diagnosis is based on the presence of steatosis,noninvasive scores and altered liver *** scores of liver fibrosis,such as serum biomarkers,which should be used in primary care to rule out advanced fibrosis,are simple,inexpensive,and widely ***,guidelines from international hepatology societies recommend using noninvasive strategies to simplify case finding and management of high-risk patients with MASLD in clinical ***,there is no definite pharmacological treatment for the *** public health policies to treat patients with risk factors for MASLD prevention is essential.

关键词： Nonalcoholic fatty liver disease Primary care Metabolic risk Liver Metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Simultaneous Raman and reflection UV/Vis absorption spectroelectrochemistry

引用

Nano Research 2022年第6期15卷 5340-5346页

作者： sheila Hernandez Juan V.Perales-Rondon Aranzazu Heras Alvaro Colina Department of Chemistry Universidad de BurgosPza.Misael Banuelos s/ñBurgos E-09001Spain Department of Analytical Chemistry Physical Chemistry and Chemical EngineeringUniversity of Alcala.Ctra.Madrid-BarcelonaKm.33.600MadridAlcaláde Henares E-28871Spain

In the present work,a new combination of Raman and ultraviolet and visible(UV/Vis)absorption spectroelectrochemistry in reflection mode is *** new experimental setup allows obtaining the two kinds of spectroscopic data without interferences concomitantly with the electrochemical *** the best of our knowledge,it is the first time to report the simultaneous obtention of electrochemical,electronic,and vibrational information in the same *** new combination provides time-resolved information about the processes that are taking place on the electrode/solution interface which has significant implications in different fields of chemistry,such as modification of electrodes,studies of electrocatalytic reaction mechanisms,development of sensors,among *** different systems were used to demonstrate the advantages and capabilities of the brand-new technique,namely,the oxidation of potassium ferrocyanide,an out-sphere system that is usually employed in the validation of SEC techniques,and the electrochemical-surface enhanced Raman spectroscopy(EC-SERS)detection of crystal violet by in-situ formation of the silver SERS substrate,where the UV/Vis spectra were used to follow the formation of the SERS substrate,whereas the Raman response of a probe molecule was used to confirm either the formation of a nanostructured surface and to obtain the fingerprint of the molecule with a high time *** brand-new experimental setup has shown to be useful,versatile,robust,compact,and easy to use for future applications.

关键词： spectroelectrochemistry surface enhanced Raman spectroscopy(SERS) Raman ultraviolet and visible(UV/Vis)absorption nanoparticles

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

eIF3 subunit M regulates blood meal digestion in Rhodnius prolixus affecting ecdysis, reproduction, and survival

引用

Insect Science 2023年第5期30卷 1282-1292页

作者： Pilar Ameijeiras Natalia Capriotti sheila Ons Pedro L.Oliveira Marcos Sterkel Laboratorio de Neurobiología de Insectos(LNI) Centro Regional de Estudios GenómicosFacultad de Ciencias ExactasUniversidad Nacional de La PlataCENEXACONICETLa PlataBuenos AiresArgentina Instituto de Bioquímica Médica Leopoldo de Meis Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular(INCT-EM) Rio de JaneiroBrazil

In triatomines,blood-feeding triggers many physiological processes including post embryonic development and *** feeding habits,such as hematophagy,can shape gene functions to meet the challenges of each type of *** gut of blood-sucking insects faces particular challenges after feeding due to the quantity and the quality of the food ingested.A comparison of transcriptomic and proteomic data indicates that post transcriptional regulation of gene expression is crucial in the triatomine *** was proposed that eukaryotic translation initiation factor 3 subunit m(eIF3m)and eIF3e define 2 different eIF3 complexes with a distinct affinity for the different mRNAs,thus selecting the set of mRNAs to be translated and constituting a post transcriptional mode of regulation of gene *** the eIF3m is mainly expressed in the gut,we evaluated its relevance in Rhodnius prolixus physiology through RNA interference-mediated gene *** knockdown of eIF3m reduced the digestion rate,affecting the processes triggered by a blood *** silencing inhibited molting and caused premature death in nymphs while impaired ovary development,oviposition and increased resistance to starvation in adult *** survival of males after feeding(resistance to starvation)was not affected by eIF3m *** information regarding the eIF3m function in insects is scarce and the phenotypes observed in *** upon eIF3m silencing are different and more severe than those previously described in Drosophila melanogaster,indicating a pleiotropic role of this gene in triatomines.

关键词： egg hatching hematophagous vectors lifespan oviposition post embryonic development starvation resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synaptic pathology in multiple sclerosis:a role for Nogo-A signaling in astrocytes?

引用

Neural Regeneration Research 2023年第1期18卷 127-128页

作者： sheila Espírito-Santo Vinícius Gabriel Coutinho Flávia Carvalho Alcantara Gomes Laboratório de Biologia Celular e Tecidual Universidade Estadual do Norte Fluminense Darcy RibeiroCampos dos GoytacazesRJBrazil Laboratório de Neurobiologia Celular Universidade Federal do Rio de JaneiroInstituto de Ciências BiomédicasRio de JaneiroRJBrazil

Multiple sclerosis(MS)is characterized as an inflammatory demyelinating disease that affects the central nervous system(CNS),leading to sensory,motor and cognitive ***,axonal denudation culminates in axonal lesions and *** demyelinating lesions in MS are associated with infiltration of immune cells combined with activation of the resident CNS inflammatory cells,astro-and microglia.

关键词： sclerosis impairment lesions

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Clinicopathological Description of a Chronic Lymphocytic Leukemia in a Dog

引用

Open Journal of Veterinary Medicine 2024年第7期14卷 155-162页

作者： Maria Clara Boni Raffi Giovanna Valverde Magalhães Barbosa Alana Carolina Capais Rodrigues sheila Canevese Rahal John P. Kastelic Luíz Guilherme Dercore Benevenuto Iolanda Simões Braga Beatriz Poloni Batista Felipe César Da Silva Brasileiro Fernanda Barthelson Carvalho de Moura Priscylla Tatiana Chalfun Guimarães Okamoto Regina Kiomi Takahira Alessandra Melchert Noeme Sousa Rocha Department of Veterinary Clinics School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Independent Veterinary Practitioner Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science So Paulo State UniversityUNESP Botucatu SP Brazil Faculty of Veterinary Medicine University of Calgary Calgary Canada

Hematopoietic tumors are associated with diagnostic and therapeutic challenges and a poor prognosis. Furthermore, their pathophysiology in dogs is not well understood. Chronic lymphocytic leukemia has been rarely reported in golden retrievers, perhaps due to difficulties in diagnosis and care. An 8-year-old spayed female golden retriever was referred to our hospital with a 2-month history of fatigue, weakness, and selective appetite. The dog was diagnosed with chronic lymphocytic leukemia based on blood smear evaluation. Despite treatment, the dog deteriorated and the owners opted for euthanasia. Histopathological examination revealed atypical lymphocytes in the spleen, extramedullary hematopoiesis in the spleen and liver, bone marrow depletion, moderate neutrophilic myocarditis, and acute renal tubular necrosis. In an immunohistochemical panel, cells in the spleen and bone marrow were positive for anti-CD20, anti-CD45, anti-CD3, anti-IBA-1, anti-Ki67, anti-MUM-1, and anti-PAX-5. In conclusion, the leukemia diagnosis was confirmed through reactive histiocytosis, marked extramedullary hematopoiesis, and other clinical and laboratory findings. Herein, we report a case of chronic lymphocytic leukemia in a female dog with clinical, laboratory, and necroscopy findings.

关键词： Canine Cancer Neoplasia Therapeutics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Foundations of officership : Military Science and leadership MSL 101 / sheila Visconti.

引用

2002年

作者： sheila Visconti.

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

Revisiting mechanisms of resistance to immunotherapies in metastatic clear-cell renal-cell carcinoma

引用

Cancer Drug Resistance 2023年第2期6卷 314-326页

作者： Monica sheila Chatwal Jad Chahoud Philippe E.Spiess Department of Genitourinary Oncology H.Lee Moffitt Cancer Center and Research InstituteTampaFL 33612USA

Renal-cell carcinoma(RCC)remains a leading cause of cancer-related mortality *** newer therapeutic combinations of immune checkpoint inhibitors and targeted therapies have greatly improved outcomes,resistance to these therapies is becoming a challenge for long-term *** of resistance have been explored in a variety of solid tumors,including *** upon our review of the current literature on the mechanisms of resistance to immunotherapies for the management of metastatic clear-cell renal cell carcinomas(mccRCC),the ensuing conclusions have been made:The management of mccRCC has progressed substantially with the advent of checkpoint inhibitors and targeted oral therapies,alone and/or in ***,innate or developed resistance to these therapies remains an ongoing challenge,particularly to immune checkpoint inhibitors(ICIs).Several of the known mechanisms of resistance have been well defined,but recent progression in cellular therapies helps to expand the armamentarium of potential combination options that may overcome these modes of resistance and improve long-term disease control and survival for an otherwise dismal *** the ensuing review and update of the literature on the mechanisms of resistance to immunotherapies in mccRCC,we have revisited the known resistance mechanisms of immunotherapies in metastatic clear-cell RCC and explored ongoing and future strategies to overcome them.

关键词： Metastatic clear-cell renal-cell carcinoma immune therapy checkpoint inhibitor resistance chimeric antigen receptor T-cell therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Surface Modification of Poly Vinyl Chloride (PVC) Using Low Pressure Argon and Oxygen Plasma

引用

Plasma Science and Technology 2010年第2期12卷 204-207页

作者： Mahmood GHORANNEVISS sheila SHAHIDI Jakub WIENER Plasma Physics Research Center Science and Research Branch Islamic Azad University Department of Textile Chemistry Faculty of Textile Technical University of Liberec

In this study, commercial poly vinyl chloride （PVC） films were treated by oxygen and argon plasmas in a cylindrical glass tube which was surrounded by a DC variable magnetic field, with different sample positions in the plasma reactor and also different exposure durations. Effects of the plasma treatment on the hydrophilic properties of the films were studied by mea- suring the water drop contact angle on the surface of the samples. The surface topography of the untreated and plasma treated films was analyzed and compared by atomic force microscopy （AFM）. The optical characteristic changes in treated samples were investigated using reflective spectrophotometry. Also, the chemical changes which appeared on the surface of the samples were investigated using Fourier transform infrared spectroscopy （FTIR）. The results show that the plasma treated PVC becomes more hydrophilic with an enhanced wettability. A sharp de- crease in the water contact angle may also be a consequence of the surface texturization. The aging effect on wettability of the samples was also investigated. The results show that the effect of oxygen plasma on the surface properties of the samples is more pronounced compared with that of argon plasma.

关键词： plasma surface modification poly vinyl chloride

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

GEX3, Expressed in the Male Gametophyte and in the Egg Cell of Arabidopsisthaliana, Is Essential for Micropylar Pollen Tube Guidance and Plays a Role during Early Embryogenesis

引用

Molecular Plant 2008年第4期1卷 586-598页

作者： Monica Alandete-Saez Mily Ron sheila McCormick Plant Gene Expression Center and Department of Plant and Microbial Biology USDA/ARS-UC-Berkeley 800 Buchanan St Albany CA 94710 USA

Double fertilization in flowering plants occurs when the two sperm cells, carried by the pollen tube, are released in a synergid cell of the embryo sac and then fertilize the egg and the central cell. Proteins on the surfaces of the sperm, egg, central, and synergid cells might be important for guidance and recognition/fusion of the gametes. Here, we present functional analyses ofArabidopsis GEX3, which encodes a plasma membrane-localized protein that has homologs in other plants. GEX3 is expressed in both the vegetative and sperm cells of the male gametophyte and in the egg cell of the female gametophyte. Transgenic lines in which GEX3 was down-regulated or overexpressed, using the Arabidopsis GEX2 promoter, had reduced seed set. Reciprocal crosses and imaging after pollination with a reporter line showed that, in both cases, the defect causing reduced seed set occurred in the female. In the antisense lines, micropylar pollen tube guidance failed, In the overexpression lines, fertilization of mutant ovules was mostly blocked because pollen tube guidance failed, although, occasionally, non-viable embryos were formed. We conclude that properly regulated expression of GEX3 in the egg cell of Arabidopsis is essential for pollen tube guidance.

关键词： antisense double fertilization female gametophyte real-time PCR plasma membrane protein PQQ domain

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：