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Development of Array-based Technology for Detection of HAV Using Gold-DNA Probes

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Development of Array-based Technology for Detection of HAV U...

Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano-gold immunological amplification and silver staining method

2006年度学术研讨会

作者： Zhixiang Wan, Yefu Wang, shawn shun-cheng li, , lianlian Duan and Jianxin Zhai Department of Biotechnology, College of life Sciences, Wuhan University, Wuhan 430072, P. R. China Department of Biochemistry, University of Western Ontario, London,Ontario N6A 5C1, Canada

A sensitive method for detection of Hepatitis A virus (HAV) by utilizing gold-DNA probe on an array was developed. Amino- modified oligodeoxynucleotides at the 5’ position were arrayed on an activated glass surface to function as capture probes. Sandwich hybridization occurred among capture probes, the HAV amplicon, and gold nanoparticlesupported oligonucleotide probes. After a silver enhancement step, signals were detected by a standard flatbed scanner or just by naked eyes. As little as 100 fM of HAV amplicon could be detected on the array. Therefore, the array technology is an alternative to be applied in detection of HAV due to its low-cost and high-sensitivity.

关键词： Array Gene detection Gold-DNA probe HAV Sensitivity

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Rapid and simultaneous detection of human hepatitis B virus ...

lithium-ion battery data and where to find it

2006年度学术研讨会

作者： lianlian Duan,Yefu Wang,shawn shun-cheng li, Zhixiang Wan and Jianxin Zhai Address:Department of Biotechnology, College of life Sciences, Wuhan University, 430072, Wuhan, Hubei, People’s Republic of China and Department of Biochemistry, University of Western Ontario, London, Ontario N6A 5C1, Canada

Background: Viral hepatitis due to hepatitis B virus and hepatitis C virus are major public health problems all over the world. Traditional detection methods including polymerase chain reaction (PCR)-based assays and enzyme-linked immunosorbent assays (EliSA) are expensive and timeconsuming. In our assay, a protein chip assay using Nano-gold Immunological Amplification and Silver Staining (NIASS) method was applied to detect HBV and HCV antibodies rapidly and simultaneously.

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Energy and AI 2021年第3期5卷 255-269页

作者： Gonçalo dos Reis Calum Strange Mohit Yadav shawn li School of Mathematics University of EdinburghThe King’s BuildingsEdinburgh EH93FDUK Centro de Matemática e Aplicações(CMA) FCTUNLQuinta da Torre2829-516 CaparicaPortugal Indian Institute of Technology KanpurIndia

lithium-ion batteries are fuelling the advancing renewable-energy based *** the core of transformational developments in battery design,modelling and management is *** this work,the datasets associated with lithium batteries in the public domain are *** review the data by mode of experimental testing,giving particular attention to test variables and data *** highlighted tools and platforms,over 30 datasets are reviewed.

关键词： lithium battery Public data Battery data Battery tests Machine learning

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

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Chinese Science Bulletin 2012年第10期57卷 1109-1115页

作者： SHI XiuJuan li Dan XIE Jing WANG shawn WU ZhaoQiang CHEN Hong College of Chemistry Chemical Engineering and Material Science Soochow University Suzhou 215123 China School of Material Science and Engineering Wuhan University of Technology Wuhan 430070 China Department of Nanotechnology Engineering University of Waterloo Waterloo N2L3G1 Canada

The interactions between bovine serum albumin(BSA) and gold nanoparticles(AuNPs) ,and the conformational changes of BSA induced by this interaction,were investigated by UV-visible absorption spectroscopy,fluorescence spectroscopy,and Fourier transform infrared in combination with attenuated total reflection spectroscopy(ATR-FTIR) .The critical adsorption density for preventing AuNP aggregation in 0.1 mol/L phosphate buffered saline(pH 7.2) was 23 BSA molecules per gold particle or 3.8×1012 BSA molecules/*** bound to the AuNPs with high affinity(binding constant Ks=7.59×108 L/mol) ,and the intrin-sic fluorescence of BSA was quenched by the AuNPs in accordance with the static quenching *** fluorescence spectroscopy and ATR-FTIR showed that AuNPs induced conformational changes in BSA,which resulted in it becoming less compact and increased the polarity of the microenvironment around the tryptophan residue Trp-212.

关键词：牛血清白蛋白金纳米粒子可见吸收光谱相互作用 ATR-FTIR 荧光光谱磷酸盐缓冲液构象变化

Transcriptome analysis of near-isogenic lines provides molecular insights into starch biosynthesis in maize kernel

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Journal of Integrative Plant Biology 2016年第8期58卷 713-723页

作者： Yingni Xiao shawn Thatcher Min Wang Tingting Wang Mary Beatty Gina Zastrow-Hayes lin li Jiansheng li Bailin li Xiaohong Yang National Maize Improvement Center of China Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100193 China DuPont Pioneer 200 Powder Mill Road Wilmington DE 19880 USA College of Agronomy Northwest Agricultural and Forest University Yang Ling 712100 China DuPont Pioneer Johnston Iowa 50131 USA Department of Agronomy and Plant Genetics University of Minnesota St. Paul Minnesota 55108 USA.

Starch is the major component in maize kernels,providing a stable carbohydrate source for humans and livestock as well as raw material for the biofuel *** maize kernel starch content will help meet industry demands and has the potential to increase overall *** developed a pair of maize near-isogenic lines（NILs） with different alleles for a starch quantitative trait locus on chromosome 3（q HS3）, resulting in different kernel starch content. To investigate the candidate genes for q HS3 and elucidate their effects on starch metabolism, RNA-Seq was performed for the developing kernels of the NILs at 14 and 21 d after pollination（DAP）. Analysis of genomic and transcriptomic data identified 76 genes with nonsynonymous single nucleotide polymorphisms and 384 differentially expressed genes（DEGs） in the in trogressed fragment, including a hexokinase gene, Zm HXK3 a, which catalyzes the conversion of glucose to glucose-6-phosphate and may play a key role instarch metabolism. The expression pattern of all DEGs in starch metabolism shows that altered expression of the candidate genes for q HS3 promoted starch synthesis,with positive consequences for kernel starch content. These results expand the current understanding of starch biosynthesis and accumulation in maize kernels and provide potential candidate genes to increase starch content.

关键词： Coexpression network maize quantitative trait loci RNA-Seq starch content

In Silico Investigation of pH-Dependence of Prolactin and Human Growth Hormone Binding to Human Prolactin Receptor

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Communications in Computational Physics 2013年第1期13卷 207-222页

作者： lin Wang shawn Witham Zhe Zhang lin li Michael Hodsdon Emil Alexov Computational Biophysics and Bioinformatics Department of PhysicsClemson UniversityClemsonSC 29634USA Department of Laboratory Medicine and the Department of Pharmacology Yale School of MedicineNew HavenConnecticut 06520USA

Experimental data shows that the binding of human prolactin(hPRL)to human prolactin receptor(hPRLr-ECD)is strongly pH-dependent,while the binding of the same receptor to human growth hormone(hGH)is *** we carry in silico analysis of the molecular effects causing such a difference and reveal the role of individual amino *** is shown that the computational modeling correctly predicts experimentally determined pKa’s of histidine residues in an unbound state in the majority of the cases and the pH-dependence of the binding free *** analysis carried in conjunction with calculated pH-dependence of the binding revealed that the main reason for pH-dependence of the binding of hPRL-hPRLr-ECD is a number of salt-bridges across the interface of the complex,while no salt-bridges are formed in the ***,most of the salt-bridges involve histidine residues and this is the reason for the pH-dependence across a physiological range of *** analysis not only revealed the molecular mechanism of the pH-dependence of the hPRL-hPRLr-ECD,but also provided critical insight into the underlying physicchemical mechanism.

关键词： Human prolactin human prolactin receptor human growth hormone pKa calculations pH-dependence electrostatics

Experimental and modeling study of de novo formation of PCDD/PCDF on MSW fly ash

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Journal of Environmental Sciences 2007年第1期19卷 117-122页

作者： li Xiao-dong ZHANG Ji YAN Jian-hua CEN Ke-fa RYAN shawn P GULLETT Brian K LEE Chunwai State key Laboratory of Clean Energy Utilization Institute for Thermal Power Engineering Zhefiang University Hangzhou 310027 China U.S. Environmental Protection Agency Office of Research and Development National Risk Management Research Laboratory E305-01 Research Triangle Park North Carolina 27711 USA

The effect of sulfur dioxide （SO2） on the formation of polychlorinated dibenzo-p-dioxins （PCDDs） and polychlorinated dibenzofurans （PCDFs） has been studied in an entrained-flow reactor （EFR） under simulated waste combustion conditions. A chlorination model based on conditional probability was employed to evaluate the homologue patterns of PCDDs and PCDFs. Results revealed that the presence of SO2 did not alter the formation pathway although SO2 suppressed PCDD/F formation. The prediction model of PCDF showed good agreement with the experimental data （R--0.95）, whereas the prediction for PCDDs did not correlate well with the experimental data. This may be explained because potential chlorination pathways play a significant role in PCDF formation, whereas PCDDs are mainly formed through condensation reactions. Furthermore, the result indicated that the steric hindrance during formation has more effects on PCDD than on PCDF due to the symmetric molecular structures of PCDDs.

关键词： PCDDs PCDFs de novo synthesis formation chlorination model sulfur dioxide air pollution waste combustion

Open-source algorithm and software for computed tomography-based virtual pancreatoscopy and other applications

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Visual Computing for Industry,Biomedicine,and Art 2022年第1期5卷 239-249页

作者： Haofan Huang Xiaxia Yu Mu Tian Weizhen He shawn Xiang li Zhengrong liang Yi Gao School of Biomedical Engineering Health Science CenterShenzhen UniversityShenzhen 518060China Laboratory for Imaging Research and Informatics State University of New YorkStony BrookNY 11794USA Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies Shenzhen 518060China Marshall Laboratory of Biomedical Engineering Shenzhen 518060China Peng cheng Laboratory Shenzhen 518066China

Pancreatoscopy plays a significant role in the diagnosis and treatment of pancreatic ***,the risk of pancreatoscopy is remarkably greater than that of other endoscopic procedures,such as gastroscopy and bronchoscopy,owing to its severe *** comparison,virtual pancreatoscopy(VP)has shown notable ***,because of the low resolution of current computed tomography(CT)technology and the small diameter of the pancreatic duct,VP has limited clinical *** this study,an optimal path algorithm and super-resolution technique are investigated for the development of an open-source software platform for VP based on 3D *** proposed segmentation of the pancreatic duct from the abdominal CT images reached an average Dice coefficient of 0.85 with a standard deviation of *** to the excellent segmentation performance,a fly-through visualization of both the inside and outside of the duct was successfully reconstructed,thereby demonstrating the feasibility of *** addition,a quantitative analysis of the wall thickness and topology of the duct provides more insight into pancreatic diseases than a fly-through *** entire VP system developed in this study is available at https://***/gaoyi/***.

关键词： Pancreatic cancer Pancreatic duct segmentation Virtual pancreatoscopy 3D Slicer

Questions about NgAgo

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Protein & Cell 2016年第12期7卷 913-915页

作者： shawn Burgess linzhao cheng Feng Gu Zhiwei Huang Shuo lin Jinsong li Wei li Wei Qin Yujie Sun Zhou Songyang Wensheng Wei Qiang Wu Haoyi Wang Xiaoqun Wang Jing-Wei Xiong Jianzhong Xi Hui Yang Bin Zhou Bo Zhang Junjiu Huang National Human Genome Research Institute NIH Bethesda MD 20892 USA Division of Hematology in Department of Medicine Johns HopkinsUniversity School of Medicine Baltimore MD 21205 USA Stem Cell Program in the Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore MD 21205 USA Center for Vision Research Eye Hospital Wenzhou Medical University Wenzhou 325035 China Stem Cell and Functional Genomics Laboratory School of LifeSciences Sun Yat-sen University Guangzhou 510275 China HIT Center of life Sciences Harbin Institute of Technology Harbin 150080 China Department of Molecular Cell and Developmental Biology University of California Los Angeles Los Angeles CA 90095 USA Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China State Key Laboratory of Stem cell and Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Chemical Biology and Biotechnology Peking Univer- sity Shenzhen Graduate School Shenzhen 518055 China PKU BIOPIC Beijing 100871 China BIOPIC ICG CLS and School of Life Sciences Peking Univer-sity Beijing 100871 China Center for Comparative Biomedicine Institute of SystemsBiomedicine Shanghai Jiao Tong University Shanghai 200240China Institute of Biophysics Chinese Academy of Sciences Beijing100101 China Institute of Molecular Medicine Peking University Beijing100871 China College of Engineering Peking University Beiiing 100871 China Laboratory of Disease Models in Non-Human Primates Instituteof Neuroscience Shanghai Chinese Academy of SciencesShanghai 200031 China College of life Sciences Peking University Beijing 100871 China

Dear Editor： Gao et al. published data in Nature Biotechnology （Nat Biotechnol. 2016 May 2） showing that DNA-guided genome editing using the Natronobacterium gregoryi Argonaute （NgAgo） protein targeted 47 mammalian genomic loci with a 100% success rate and an efficiency of 21.3%-41.3% at various targets. This report led us to test NgAgo＇s utility in various cells and organisms such as mouse and zebrafish for gene editing.

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