检索结果-南通市图书馆

Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第5期66卷 1151-1169页

作者： Hong-Yan Wang Xiang liu Jian-Yang Chen Yingyi Huang Yifang Lu Fujian Tan Qun liu Mingming Yang Shuo Li Xianghui Zhang Yating Qin Wenxiu Ma Yingming Yang Liang Meng Kaiqiang liu Qian Wang Guangyi Fan Rafael H.Nóbrega shanshan liu Francesc Piferrer Changwei Shao Key Lab of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266072China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China BGI Research-Qingdao BGIQingdao 266555China BGI-Shenzhen Shenzhen 518083China School of Marine Sciences Ningbo UniversityNingbo 315211China College of Marine Technology and Environment Dalian Ocean UniversityDalian 116023China Qingdao-Europe Advanced Institute for Life Sciences BGI-ShenzhenQingdao 266555China College of Life Sciences Shandong Normal UniversityJinan 250014China Reproductive and Molecular Biology Group Department of Structural and Functional BiologyInstitute of BiosciencesSão Paulo State University(UNESP)BotucatuSão PauloBrazil Institut de Ciències del Mar(ICM) Spanish National Research Council(CSIC)BarcelonaSpain

Female-to-male sex reversals(pseudomales)are common in lower vertebrates and have been found in natural populations,which is a concern under rapid changes in environmental *** can exhibit altered ***,the regulatory mechanisms underlying pseudomale spermatogenesis remain ***,we characterized spermatogenesis in Chinese tongue sole(Cynoglossus semilaevis),a species with genetic and environmental sex determination,based on a highresolution single-cell RNA-seq atlas of cells derived from the testes of genotypic males and *** identified five germ cell types and six somatic cell types and obtained a single-cell atlas of dynamic changes in gene expression during spermatogenesis in Chinese tongue sole,including alterations in *** detected decreased levels of Ca^(2+)signaling pathway-related genes in spermatogonia,insufficient meiotic initiation in spermatocytes,and a malfunction of somatic niche cells in ***,a cluster of CaSR genes and MAPK signaling factors were upregulated in undifferentiated spermatogonia of ***,we revealed that Z chromosome-specific genes,such as piwil2,dhx37,and ehmt1,were important for *** results improve our understanding of reproduction after female-to-male sex-reversal and provide new insights into the adaptability of reproductive strategies in lower vertebrates.

关键词： Cynoglossus semilaevis spermatogenesis single-cell transcriptome sex reversal spermatogonia

Improving electrochemical activity of PtRu/SnO_2/C catalyst by reduction treatment and alkaline etching

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2016年第5期25卷 811-816页

作者： Qi Wang Hualong Tao Zhiqiang Li Chunhuan Chen shanshan liu Lei Han Xing Lu Liaoning Key Materials Laboratory for Railway School of Materials Science and Engineering Dalian Jiaotong University

PtRu/SnO/C catalyst was prepared in a polyol process, followed by reduction treatment and alkaline etching. X-ray diffraction, transmission electron microscope with energy dispersive spectrometer and Xray photoelectron spectroscopy were used to characterize the morphology, structure and composition of the catalysts. CO and methanol electro-oxidation activities of the catalysts were evaluated by CO stripping voltammetry, cyclic voltammetry and chronoamperometry measurements. Reduction treatment of the prepared PtRuSnO/C catalyst in a polyol process induced the enrichment of Sn on the surface, inhibiting methanol dissolution and CO adsorption on Pt. Alkaline etching removed Sn or SnOand thus exposed PtRu on the surface, resulting in enhanced activities for CO and methanol electro-oxidation due to the synergy effects of PtRu on the surface and Sn species beneath.

关键词： Platinum Ruthenium Tin oxide CO tolerance Methanol electro-oxidation

基于效应面法优化酒石酸美托洛尔择时释药微丸处方

在线全文

学校读者我要写书评

暂无评论

中国现代应用药学 2021年第22期38卷 2858-2863页

作者：刘珊珊江苏经贸职业技术学院健康学院南京211168

目的制备酒石酸美托洛尔择时释药微丸。方法根据溶胀-爆破原理,以交联羧甲基纤维素钠为溶胀层材料、乙基纤维素为控释层材料,采用流化床底喷技术制备酒石酸美托洛尔择时释药微丸;采用Box-Behnken效应面法以时滞时间及时滞后药物释放度... 详细信息

目的制备酒石酸美托洛尔择时释药微丸。方法根据溶胀-爆破原理,以交联羧甲基纤维素钠为溶胀层材料、乙基纤维素为控释层材料,采用流化床底喷技术制备酒石酸美托洛尔择时释药微丸;采用Box-Behnken效应面法以时滞时间及时滞后药物释放度为考察指标优化其处方。结果溶胀层、控释层的厚度及致孔剂的用量对微丸的时滞和释药速率均有显著影响。按最优处方制备的微丸,其体外释药时滞为4 h,时滞后2 h累积释药量>85%。结论效应面优化处方所制备的酒石酸美托洛尔择时释药微丸达到预期的择时释药目的。

关键词：效应面法酒石酸美托洛尔择时释药溶胀-爆破原理微丸

Wet Oxidation Pretreatment of Poplar Waste for Enhancing Enzymatic Hydrolysis Efficiency

在线全文

学校读者我要写书评

暂无评论

Paper And Biomaterials 2017年第2期2卷 8-17页

作者： GuiGan Fang shanshan liu KuiZhong Shen Yan Lin Institute of Chemical Industry of Forestry Products CAFNational Engineering Lab for Biomass Chemical UtilizationKey Lab of Forest Chemical EngineeringSFAKey Lab of Biomass Energyand Material of JiangsuNanjingJiangsu Province210042China Institute of New Technology of Forestry CAFBeijing100091China Collaboration Innovation Center for High Efficient Processing and Utilization of Forestry Resources Nanjing Forestry UniversityNanjingJiangsu Province210037China Qilu University of Technology Ji'nanShandong Province250353China

In this paper, we described the optimization of the wet oxidation pretreatment conditions to enhance enzymatic hydrolysis efficiency, using poplar waste from the stock section of a paper mill as the raw material. We showed that the optimal conditions of the pretreatment for poplar waste were an initial p H value of 10, a temperature of 195℃, a holding time of 15 min, and an oxygen pressure of 1.2 MPa. In this case, the yield of the obtained solid material produced by the process was 51.7% and the reducing sugar yield was 46.8%. The solid part obtained from the pretreatment process was hydrolyzed by cellulase L-10. The optimal enzymatic conditions were a temperature of 49℃, a duration time of 56 h, an enzyme dosage of 38 FPU/g at a p H value of 4.8, and a solid-to-liquor ratio of 1∶50. The resulting cellulose conversion rate reached 96.4% in terms of the pretreated substances. In addition, a chemical composition analysis of the poplar waste and pretreated material indicated that about 92% of the hemicelluloses and 43% of the lignin in the raw material were degraded and dissolved. In addition, the crystallization decreased from 57.5% to 54.8%. An obvious fibrillation of the fiber pretreated by the wet oxidization process was observed by SEM. Moreover, high-performance liquid chromatography(HPLC) results showed a high xylose content and monosaccharide degradation products in the pretreatment solution. In conclusion, the wet oxidation pretreatment process could efficiently degrade or remove the lignin and hemicellulose, as well as reduce the crystallinity of the lignocellulosic material, which resulted in animprovement of the enzymatic ability and an increase in the cellulose conversion rate.

关键词： poplar waste wet oxidation pretreatment enzymatic hydrolysis efficiency cellulase hydrolysis

在线全文

学校读者我要写书评

暂无评论

解释论视角下的行贿罪特殊从宽处罚条款

社会科学 2020年第5期 102-110页

作者：刘山煽最高人民法院刑二庭中国政法大学北京100062

《刑法修正案(九)》对行贿罪特殊从宽处罚条款进行了修订,对行贿罪从宽处罚增加了适用条件。基于历史解释、体系解释和目的解释的不同解释方法,适用行贿罪特殊从宽处罚条款应一方面服务于打击贿赂犯罪大局,另一方面起到平衡行受贿量刑... 详细信息

《刑法修正案(九)》对行贿罪特殊从宽处罚条款进行了修订,对行贿罪从宽处罚增加了适用条件。基于历史解释、体系解释和目的解释的不同解释方法,适用行贿罪特殊从宽处罚条款应一方面服务于打击贿赂犯罪大局,另一方面起到平衡行受贿量刑的作用,同时标准应尽可能明确以避免放纵行贿犯罪。故此,对该条款中"主动交待"宜虚化处理,"犯罪较轻"应以犯罪可能适用的刑罚为判断标准,对"重大案件"、"重大立功表现"均应作扩大解释。

关键词：行贿罪特殊从宽处罚条款历史解释体系解释目的解释司法适用

Flexible molecules dedicate to release strain of inverted inorganic perovskite solar cell

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 87-93页

作者： Hongrui Sun Sanlong Wang Pengyang Wang Yali liu shanshan Qi Biao Shi Ying Zhao Xiaodan Zhang Institute of Photoelectronic Thin Film Devices and Technology Renewable Energy Conversion and Storage Center State Key Laboratory of Photovoltaic Materials and CellsNankai University Key Laboratory of Photoelectronic Thin Film Devices and Technology of Tianjin Haihe Laboratory of Sustainable Chemical Transformations Engineering Research Center of Thin Film Photoelectronic Technology of Ministry of Education Collaborative Innovation Center of Chemical Science and Engineering (Tianjin)

The tensile strain in inorganic perovskite films induced by thermal annealing is one of the primary factors contributing to the inefficiency and instability of inorganic perovskite solar cells （IPSCs）,which reduces the defect formation ***,a flexible molecule 5-maleimidovaleric acid （5-MVA） was introduced as a strain buffer to release the residual strain of *** anhydride and carboxyl groups in 5-MVA interact strongly with the uncoordinated Pb2+through Lewis acid-base reaction,thus tightly“pull”the perovskite *** in-between soft carbon chain increased the structural flexibility of CsPbI2.85Br0.15perovskite materials,which effectively relieved the intrinsic internal strain of CsPbI2.85Br0.15,resisted the corrosion of external strain,and also reduced the formation of defects such as VIand *** addition,the introduction of 5-MVA improved crystal quality,passivated residual defects,and narrowed energy level ***,power conversion efficiency （PCE） of NiOxbased inverted IPSCs increased from 19.25% to 20.82% with the open-circuit voltage enhanced from 1.164 V to 1.230 *** release of strain also improved the stability of CsPbI2.85Br0.15perovskite films and devices.

关键词： Inverted inorganic perovskite solar cells Flexible molecules Strain release Crystallization Energy barrier High PCE

Comparison of DNA Extraction Methods for Four Kinds of Seafood in Qinhuangdao

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Agricultural Biotechnology 2022年第3期11卷 1-5,17页

作者： shanshan liu Haijuan ZHANG Weiwei WANG Dongmei ZHAO Zhicheng WANG Ying liu Qinhuangdao Food and Drug Inspection Center Qinhuangdao 066000China Hebei Normal University of Science and Technology Qinhuangdao 066000China Hebei Drug Evaluation Center Shijiazhuang 050000China

[Objectives]This study was conducted for the purpose of widely using molecular biology techniques in the detection of seafood species.[Methods]In this study,such four kinds of seafood as fish,shrimps,crabs and shellfish were used as samples to compare three DNA extraction methods,including Tiangen marine animal tissue genome extraction kit method(DP324)(a),SN/T 3589.6-2013/8.3(b)method and modified SN/T 3730.8-2013/8.1(c)*** purity and concentrations of DNA and the amplification effects of real-time fluorescene PCR were compared.[Results]The three methods had different extraction effects while satisfying the real-time fluorescent PCR *** DNA templates extracted by the SN/T 3730.8-2013/8.1 standard method had higher purity and concentrations,and showed clearer bands in gel electrophoresis,indicating that the method has less effect on real-time fluorescent PCR and less inhibition,and can meet the detection needs of the four types of seafood,including fish,shrimps,crabs and shellfish.[Conclusions]This study provides a theoretical basis for the detection of seafood animal-derived components.

关键词： Seafood DNA extraction Real-time fluorescence PCR Detection

Extreme drought changes in Southwest China from 1960 to 2009

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Geographical Sciences 2013年第1期23卷 3-16页

作者： ZHANG Mingjun HE Jinyun WANG Baolong WANG Shengjie LI shanshan liu Wenli MA Xuening College of Geography and Environment Sciences Northwest Normal University Lanzhou 730070 China

Based on the daily data of temperature and precipitation of 108 meteorological stations in Southwest China from 1960 to 2009, we calculate the monthly and yearly surface humid indexes, as well as the extreme drought frequency. According to the data, the temporal and spatial characteristics of the extreme drought frequency in inter-annual, inter-decadal, summer monsoon period and winter monsoon period are analyzed. The results are indicated as follows. （1） In general, the southwestern Sichuan Basin, southern Hengduan Mountains, southern coast of Guangxi and northern Guizhou are the areas where the extreme drought frequency has significantly increased in the past 50 years. As for the decadal change, from the 1960s to the 1980s the extreme drought frequency has presented a decreasing trend, while the 1990s is the wettest decade and the whole area is turning wet. In the 2000s, the extreme drought frequency rises quickly, but the regional differences reduce. （2） During summer monsoon period, the extreme drought frequency is growing, which generally occurs in the high mountains around the Sichuan Basin, most parts of Guangxi and ＂the broom-shaped mountains＂ in Yunnan. It is distinct that the altitude has impacts on the ex- treme drought frequency; during winter monsoon period, the area is relatively wet and the extreme drought frequency is decreasing. （3） During summer monsoon period, the abrupt change is observed in 2003, whereas the abrupt change during winter monsoon period is in 1989. The annual extreme drought frequency variation is a superposition of abrupt changes during summer monsoon and winter monsoon periods. The departure sequence vibration of annual extreme drought frequency is quasi-5 years and quasi-12 years.

关键词： Southwest China extreme drought summer monsoon winter monsoon

Effect of Apigenin on Gap Junctional Intercellular Communication in Human Tenon's Capsule Fibroblasts

在线全文

学校读者我要写书评

暂无评论

Eye Science 2013年第2期28卷 62-67页

作者： shanshan liu Jibing Wang Huihui Zou Xudong Huang Department of Ophthalmology Weifang Medical University Weifang Eye Hospital National Key Clinical Constructive Project Unit of Ophthalmology Department of Ophthalmology Dezhou People's Hospital

Purpose:To investigate the effect of apigenin on gap junctional intercellular communication (GJIC) in human Tenon's capsule fibroblasts (HTFs) and its underlying mechanism. Methods:After a 48 h treatment of cultured HTFs with apigenin.(80 μmol/L),the GJIC was detected by a scrape-loading/dye transfer technique with Lucifer yellow dye and rhodamine (Rh) dextran. The coupling index represents a quantification of GJIC where a high coupling index is associated with a greater number of cells demonstrating cell-cell communication through gap junction *** changes in connexin 43 (Cx43) distribution and the expression of Cx43 at the protein and mRNA levels were statistically compared between the two groups by means of immunocytochemistry, western blotting,and real-time polymerase chain reaction (PCR). Results:The functioning of GJIC in the HTFs was significantly enhanced after 48 hours by apigenin treatment when compared with the control cells. In the apigenin group, the intercellular dye transfer grade was above 9, while this value was only grade 3-4 in the control group. The coupling index was significantly increased up to 9.205±0.3621 in the apigenin group,compared with 5.1775 ±0.3177 in the control group (F=279.581, P=0.000). The expression of Cx43 at the protein and mRNA levels was significantly up-regulated in the apigenin group compared with the control group. Conclusion:Apigenin can significantly enhance the function of GJIC in HTFs by up-regulating the expression of Cx43 at both the protein and mRNA levels,suggesting that the enhancement of GJIC in HTFs by apigenin probably acts as an important mechanism underlying the inhibitory effect of apigenin on HTF proliferation.

关键词：细胞间隙连接通讯成纤维细胞芹菜素 mRNA水平 Cx43 连接蛋白免疫细胞化学聚合酶链反应