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Silencing Bmi-1 enhances the senescence and decreases the metastasis of human gastric cancer cells

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World Journal of Gastroenterology 2013年第46期19卷 8764-8769页

作者： Feng-Lan Gao wei-shan li Chun-ling liu Guo-Qiang Zhao Pathological Examination and Research Center Luohe Medical College Basic Medical Science Institute Zhengzhou University

AIM:To evaluate the impact of Bmi-1 on cell senescence and metastasis of human gastric cancer cell line ***:Two pairs of complementary small hairpin RNA(shRNA)oligonucleotides targeting the Bmi-1gene were designed,synthesized,annealed and cloned into the pRNAT-U6.2 *** DNA sequencing to verify the correct insertion of the shRNA sequences,the recombinant plasmids were transfected into BGC823 *** expression of Bmi-1 mRNA and protein was examined by reverse transcription-polymerase chain reaction(RT-PCR)and Western *** effects of Bmi-1 knockdown on cell senescence and metastasis were determined by theβ-Gal activity assay and Boyden chamber assay,***:The double-stranded oligonucleotide fragments of Bmi-1 short interfering RNA(siRNA)cloned into pRNAT-U6.2 vector conformed to the inserted ***-PCR and Western blotting indicated that the expression levels of Bmi-1 gene mRNA and protein were markedly decreased in transfected BGC823 cells with pRNAT-U6.2-si1104 and pRNATU6.2-si1356,especially in transfected BGC823 cells with pRNAT-U6.2-si1104,compared with two control groups(empty vector and blank group).In particular,Bmi-1 protein expression was almost completely abolished in cells transfected with the recombinant vector harboring shRNA targeting the sequence GGAGGAGGTGAATGATAAA(nt1104-1122).Compared with untransfected cells and cells transfected with the empty vector,the mean percentage of senescent cells increased and the number of cells passing through the Matrigel decreased in cells transfected with the recombinant ***:Silencing Bmi-1 by RNA interference can increase the senescent cell rate and effectively reduce the metastasis of gastric cancer cells.

关键词： Bmi-1 Gastric cancer Senescence Metas-tasis

Synthesis of dandelion-like TiO_2 microspheres as anode materials for lithium ion batteries with enhanced rate capacity and cyclic performances

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International Journal of Minerals,Metallurgy and Materials 2012年第11期19卷 1058-1062页

作者： Jin Yi Yan-lin liu Yuan Wang Xiao-ping li She-jun Hu wei-shan li School of Chemistry and Environment South China Normal University Guangzhou 510006 China Key Laboratory of Electrochemical Technology on Energy Storage and Power Generation of Guangdong Higher Education Institutes Guangzhou 510006 China Engineering Research Center of Materials and Technology for Electrochemical Energy Storage Ministry of Education Guangzhou 510006 China

Dandelion-like TiO2 microspheres consisting of numerous rutile single-crystalline nanorods were synthesized for the first time by a hydrothermal method. Their crystal structure, morphology and electrochemical properties were characterized by X-ray diffraction （XRD）, scanning electron microscopy （SEM）, transmission electron microscopy （TEM）, and galvanostatic charge and discharge tests. The results show that the synthesized TiO2 microspheres exhibit good rate and cycle performances as anode materials of lithium ion batteries. It can be found that the dandelion-like structure provides a larger specific surface area and the single-crystalline nanorod provides a stable structure and fast pathways for electron and lithium ion transport, which contribute to the rate and cycle performances of the battery.

关键词： titanium dioxide microspheres nanorods anodes lithium batteries

Therapeutic effect of autologous concentrated growth factor on lower-extremity chronic refractory wounds:A case report

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World Journal of Clinical Cases 2021年第18期9卷 4797-4802页

作者： Po liu Yang liu Chang-Neng Ke wei-shan li Yue-Ming liu Shi Xu Department of Burn and Plastic Surgery Shenzhen Longhua District Central HospitalShenzhen 518110Guangdong ProvinceChina

BACKGROUND Management of chronic refractory wounds is one of the toughest clinical challenges for *** of poor blood supply,less tissue coverage,and easy exposure,the lower leg is a common site for chronic refractory *** current therapeutic regimens often lead to prolonged hospital stay and higher healthcare *** growth factor(CGF)is a novel blood extract that contains various growth factors,platelets,and fibrins to promote wound healing ***,there has been little research reported on the treatment of lower extremity wounds with *** SUMMARY A 37-year-old man,without any past medical history,presented an ulcerated chronic wound on his right lower *** skin defect exhibited clear boundaries,with a size of 2.0 cm×3.5 *** depth of wound was up to the layer of deep *** aureus was detected by bacterial *** final diagnosis was right lower extremity ulcers with ***,silver sulfadiazine,and mupirocin cream were applied to control the *** gel was prepared from the patient’s blood sample,and was used to cover the wound after thorough *** skin wound was successfully healed after three times of CGF *** CGF displays an excellent wound healing promoting effect in patients with lowerextremity chronic refractory wounds.

关键词： Concentrated growth factors Chronic refractory wounds Lower extremity Ulcer Wound healing Case report

Microstructure analysis of deep rock in Meilinmiao Mine

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Journal of Coal Science & Engineering(China) 2013年第4期19卷 468-473页

作者： Ren-liang shan li-wei SONG Yan liU Wen-Feng ZHAO Hui-Xian CHEN School of Mechanics and Civil Engineering China University of Mining and Technology (Beijing) Beijing 100083 China

SEM tests were conducted on four kinds of typical deep rocks in Meilinmiao Mine, and the micro properties were analyzed. The particles of gritstone are unbroken and particle diameter ranges from 80 lam to 400 pro, and the void between particles is wide. The macroscopic failure of gritstone is caused by breakage of the dung distributed between particles, and po- tassium feldspars contained in gritstone are not leached. The failure of medium sandstone is caused by micro cracks distributed in sandstone particles, and potassium feldspars are leached seriously, so the pores penetrate each other and there is close hy- draulic connection in medium sandstone. The particles of fine sandstone are broken, and the particle diameters range from 60 pm to 300 pm, which determines the pores have good connectivity between particles. The dolomites contained in fine sand- stone are corroded, and the micro fracture form belongs to inter-granular fracture. There are no particles and blocks in micro- structure of sandy mudstone, but there are diverse micro cracks. The failure of mudstone arises from the extension of micro cracks, and the microstructure is different from the other three kinds of sandstones obviously.

关键词： SEM test deep rock microstructure leaching crack

Relationship between Trp64Arg mutation in the β3-adrenergic receptor gene and metabolic syndrome： a seven-year follow-up study

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Chinese Medical Journal 2010年第17期123卷 2375-2378页

作者： ZHU Lue-yun HU li-ye li Xiao-ling WANG Guang-yu shan wei MA li-cheng WANG Xiu-hui Department of Endocrinology Bethune International Peace Hospital Shijiazhuang Hebei 050082 China

Background It has been shown that the β3-adrenergic receptor （β3-AR） gene Trp64Arg mutation was closely related to obesity and insulin resistance, and may be related to the prevalence of metabolic syndrome （MS）. The aim of this study was to investigate the relationship between the 33-AR gene mutation and the prevalence of MS. Methods A seven-year follow-up study was initiated in 2000, with 496 samples of simplex obese subjects （body mass index ≥25 kg/m2） and 248 normal-weight subjects. According to the β3-AR genotypes, the subjects were classified as Trp64 homozygote group and Arg64 carrier group and after 7 years the prevalence of MS was determined. Results According to the baseline profile, there were no significant differences in the adiposity, blood pressure, lipid profile, fasting plasma glucose and fasting insulin between Trp64 homozygote group and Arg64 carrier group either in obesity or normal-weight subjects. The results of follow-up study indicated that in obese men the prevalence rate of MS was much higher in Arg64 carrier group than that in Trp64 homozygote group （54.76% vs. 40.85%, P 〈0.05）, but there was no statistical difference in women of the above groups. The prevalence rate of MS in obese men of both Trp64 homozygote group and Arg64 carrier obese group were obviously higher than that in women of the above groups （40.85% vs. 18.27% and 54.76% vs 21.28%, all P 〈0.005）. Differences were not statistically significant in the prevalence of MS for normal weight Trp64 homozygote group and normal weight Arg64 carrier group, either between men, between women, or between men and women. Comparison of populations indicated that no matter with the β3-AR gene mutation or not, the prevalence of MS in obese subjects was significantly higher than normal weight subjects （X2=28.240 and x2=15.586, all P 〈0.005）. Logistic analysis showed that the mutation of β3-AR gene was associated with the prevalence of MS in men.

关键词： metabolic syndrome β3-adrenergic receptor polymorphism prospective study

Effects of AT1 receptor antagonist,Iosartan,on rat hepatic fibrosis induced by CCl_4

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World Journal of Gastroenterology 2000年第4期6卷 540-545页

作者： Hong shan wei Ding Guo li Han Ming Lu Yu Tao Zhan Zhi Rong Wang Xin Huang Jing Zhang Ji lin Cheng Qin Fang Xu Department of Gastroenterology,Xinhua Hospital,shanghai Second Medical University,shanghai 200092,China 上海第二医科大学上海

AIM To investigate effect of losartan,an AT1receptor antagonist,on hepatic fibrosis induced byCCl;and to determine whether or not AT1receptors are expressed on hepatic stellate cells,METHODS AND RESULTS Fifty male Sprague-Dawley rats,weighing（180±20）g,wererandomized into five groups（control group,modelgroup,and three losartan treated groups）,inwhich all rats were given the subcutaneousinjection of 40% CCl4（every 3 days for 6 weeks）except for rats of control *** of losartan-treated groups were treated with losartan（20 mg/kg,10 mg/kg,5 mg/kg,daily gavage）,After 6weeks liver tissue and serum samples of all ratswere *** hyaluronic acid（HA）,procollagen typeⅢ（PCⅢ）were detected byradioimmunoassays,van Giesion collagen stainingwas used to evaluate the extracellular matrix of ratswith liver *** expression of AT1receptors,transforming growth factor-beta（TGF-β）,and alpha-smooth muscle actin（a-SMA）inliver tissue were determined byimmunohistochemical *** withmodel group,serum ALT and AST of losartan-treated groups were significantly reduced（t=4.20,Pliver *** AT1 receptor antagonist,losartan,could limit the progression of the hepatic fibrosisinduced by CCl4.The mechanism may be related tothe decrease in the expression of AT1 receptorsand TGF-β,ameliorating the injury of hepatocytes;activation of local renin-angiotensin system mightrelate to hepatic fibrosis;and during progressionof fibrosis,activated hepatic stellate cells mightexpress AT1 receptors.

关键词： liver cirrhosis/drug therapy renin-angiotensin system angiotensin Ⅱ type 1 receptor antagonist losartan

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家族企业实际控制人的任期如何影响企业绩效

当代财经 2016年第3期 65-76页

作者：杨超山立威西南财经大学经济与管理研究院四川成都611130

从隧道行为和企业投资出发,基于我国上市家族企业的面板数据,深入探讨实际控制人的任期对企业绩效的影响机制。实证研究发现,家族企业实际控制人的任期与隧道行为、企业投资都呈倒U型关系,即随着实际控制人任期的增加,上市家族企业的隧... 详细信息

从隧道行为和企业投资出发,基于我国上市家族企业的面板数据,深入探讨实际控制人的任期对企业绩效的影响机制。实证研究发现,家族企业实际控制人的任期与隧道行为、企业投资都呈倒U型关系,即随着实际控制人任期的增加,上市家族企业的隧道行为减少,投资水平增加,投资更趋稳定,投资不足减少。不过,实际控制人任期过长,导致家族企业的隧道行为增加,投资水平降低。实证结果还发现,实际控制人的任期对隧道行为、企业投资的影响反映在企业绩效上,使得实际控制人的任期与企业绩效也呈倒U型关系,并在15年左右达到峰值。

关键词：实际控制人任期隧道行为企业投资企业绩效

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中国与欧美国家农药管理制度比较分析

农药 2015年第6期54卷 464-468页

作者：张薇单炜力农业部农药检定所北京100125

全面介绍了美国、欧盟在农药管理方面的制度、评价体系、技术标准、知识产权保护等,梳理我国农药管理中的突出问题,强调围绕新《农药管理条例》配套规章制修订,应考虑到国家的经济发展阶段和历史背景。针对我国农药产业发展与管理现状,... 详细信息

全面介绍了美国、欧盟在农药管理方面的制度、评价体系、技术标准、知识产权保护等,梳理我国农药管理中的突出问题,强调围绕新《农药管理条例》配套规章制修订,应考虑到国家的经济发展阶段和历史背景。针对我国农药产业发展与管理现状,提出4点建议:一是构建具有中国特色的新的登记管理法规体系;二是系统、全面引入风险评估机制;三是加强知识产权保护力度;四是引导社会广泛参与和监督。

关键词：农药登记管理比较

Acute and chronic excitotoxicity in ischemic stroke and late-onset Alzheimer's disease

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Neural Regeneration Research 2025年第7期20卷 1981-1988页

作者： shan Ping Yu Emily Choi Michael QJiang ling wei Department of Anesthesiology Emory University School of MedicineAtlantaGAUSA Center for Visual and Neurocognitive Rehabilitation Atlanta Veterans Affairs Medical CenterDecaturGAUSA

Stroke and Alzheimer's disease are common neurological disorders and often occur in the same *** comorbidity of the two neurological disorders represents a grave health threat to older *** review presents a brief background of the development of novel concepts and their clinical *** activity of glutamatergic N-methyl-D-aspartate receptors and N-methyl-D-aspartate receptor-mediated Ca^(2+)influx is critical for neuronal *** ischemic insult induces prompt and excessive glutamate release and drastic increases of intracellular Ca^(2+)mainly via N-methyl-D-aspartate receptors,particularly of those at the extrasynaptic *** Ca^(2+)-evoked neuronal cell death in the ischemic core is dominated by necrosis within a few hours and days known as acute ***,mild but sustained Ca^(2+)increases under neurodegenerative conditions such as in the distant penumbra of the ischemic brain and early stages of Alzheimer's disease are not immediately toxic,but gradually set off deteriorating Ca^(2+)-dependent signals and neuronal cell loss mostly because of activation of programmed cell death *** on the Ca^(2+)hypothesis of Alzheimer's disease and recent advances,this Ca^(2+)-activated“silent”degenerative excitotoxicity evolves from years to decades and is recognized as a unique slow and chronic *** N-methyl-D-aspartate receptor subunit GluN3A,primarily at the extrasynaptic site,serves as a gatekeeper for the N-methyl-D-aspartate receptor activity and is neuroprotective against both acute and chronic *** stroke and Alzheimer's disease,therefore,share an N-methyl-D-aspartate receptor-and Ca^(2+)-mediated mechanism,although with much different time *** is thus proposed that early interventions to control Ca^(2+)homeostasis at the preclinical stage are pivotal for individuals who are susceptible to sporadic late-onset Alzheimer's disease and Alzheimer's disease-related

关键词： Ca^(2+)hypothesis cognitive deficits hyperactivity late-onset Alzheimer's disease neurodegeneration N-methyl-D-aspartate receptors N-methyl-D-aspartate receptor subunits pathogenesis preventive treatment