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Noncovalent reversible binding-enabled facile fabrication of leak-free PDMS microfluidic devices without plasma treatment for convenient cell loading and retrieval

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Bioactive Materials 2022年第10期7卷 346-358页

作者： Bin Jiang Alisa White Wenquan ou Sarah Van Belleghem Samantha Stewart James G.Shamul shaik o.rahaman John P.Fisher Xiaoming He Fischell Department of Bioengineering University of MarylandCollege ParkMD20742USA Department of Nutrition and Food Science University of MarylandCollege ParkMD20742USA Marlene and Stewart Greenebaum Comprehensive Cancer Center University of MarylandBaltimoreMD21201USA

The conventional approach for fabricating polydimethylsiloxane(PDMS)microfluidic devices is a lengthy and inconvenient procedure and may require a clean-room microfabrication facility often not readily ***,living cells can’t survive the oxygen-plasma and high-temperature-baking treatments required for covalent bonding to assemble multiple PDMS parts into a leak-free device,and it is difficult to disassemble the devices because of the irreversible covalent *** a result,seeding/loading cells into and retrieving cells from the devices are ***,we discovered that decreasing the curing agent for crosslinking the PDMS prepolymer increases the noncovalent binding energy of the resultant PDMS surfaces without plasma or any other *** enables convenient fabrication of leak-free microfluidic devices by noncovalent binding for various biomedical applications that require high pressure/flow rates and/or long-term cell culture,by simply hand-pressing the PDMS parts without plasma or any other treatment to bind/*** this method,multiple types of cells can be conveniently loaded into specific areas of the PDMS parts before assembly and due to the reversible nature of the noncovalent bonding,the assembled device can be easily disassembled by hand peeling for retrieving *** with 3D printers that are widely available for making masters to eliminate the need of photolithography,this facile yet rigorous fabrication approach is much faster and more convenient for making PDMS microfluidic devices than the conventional oxygen plasma-baking-based irreversible covalent bonding method.

关键词： Binding energy 3D printing Soft lithography Microfluidics Polydimethylsiloxane

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Interfacial modulation of bifunctional electrolyte additive engineering for dendrite-free and robust lithium metal anode

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Journal of Energy Chemistry 2024年第10期97卷 120-127,I0003页

作者： Mahammad Rafi shaik Yongmin Park Young-Kwang Jung Won Bin Im Division of Materials Science and Engineering Hanyang University222Wangsimni-roSeongdongguSeoul 0473Republic of Kor Department of Chemical Engineering and Biotechnology University of CambridgeCambridge CB3 OASUK

Anode materials for rechargeable electric car batteries are obtained from Li-metal owing to their extremely high specific capacity and low redox ***,safety concerns related to dendrite formation on the anode surface caused by the uneven distribution of Li-ions during the discharge process interfere with the use of Li-metal in industrial *** this study,methyl vinyl sulfone(MVS),a sulfone-based functional electrolyte additive,is used in an additive engineering strategy to control Lielectrolyte interactions and address the aforementioned *** dendrite growth may be restricted,and transition metal degradation on the surface of the cathode can be reduced by the MVS-derived functional electrolyte additive interfacial *** electrochemical performance of an ethylene carbonate/dimethyl carbonate(EC/DMC)+1 wt% MVS Li-metal anode of a Li||Li symmetric cell exhibits remarkable cycle stability,maintaining a low overvoltage for over 750 h at 1 mA cm^(-2),and capacity of 1 mA h cm^(-2).Additionally,LiNi_(0.8)Co_(0.1)Mn_(0.1)o_(2)(NCM811) full cells with the MVS additive exhibit enhanced electrochemical stability for 250 cycles at a current density of 100 mA g^(-1).This study provides an innovative approach for stabilizing the metal-electrolyte interfacial layer that may be used for practical applications in metal-based rechargeable batteries.

关键词： Lithium rechargeable battery Dendrite-free Electrolyte additive Bifunctional electrolyte Interfacial layer

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Isolation of Microcrystalline Cellulose from Wood and Fabrication of Polylactic Acid(PLA)Based Green Biocomposites

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Journal of Renewable Materials 2024年第8期12卷 1455-1474页

作者： Selwin Maria Sekar Rajini Nagarajan Ponsuriyaprakash Selvakumar Ismail Sikiru oluwarotimi Kumar Krishnan Faruq Mohammad Mohammed Rafi shaik Nadir Ayrilmis Department of Mechanical Engineering Kalasalingam Academy of Research and EducationKrishnankoil626126India Department of Mechanical Engineering SSM Institute of Engineering and TechnologyDindigul624002India Department of Engineering Centre for Engineering ResearchSchool of PhysicsEngineering and Computer ScienceUniversity of HertfordshireHatfieldAL109ABUK INTI International University Persiaran Perdana BBNNilai71800Malaysia Department of Chemistry College of ScienceKing Saud UniversityP.O.Box 2455Riyadh11451Kingdom of Saudi Arabia Department of Wood Mechanics and Technology Faculty of ForestryIstanbul University–CerrahpasaBahcekoySariyerIstanbul34473Turkey

An innovative microcrystalline cellulose(MCC)natural fibre powder-reinforced PLA biocomposite was investigated using the hand lay-up *** polymer matrix composite(PMC)samples were prepared by varying the weight percentages(wt.%)of both PLA matrix and MCC reinforcement:pure PLA/100:0,90:10,80:20,70:30,60:40 and 50:50 wt.%,*** the results obtained,MCC powder,with its impressive aspect ratio,proved to be an ideal reinforcement for the PLA,exhibiting exceptional mechanical *** was evident that the 80:20 wt.%biocomposite sample exhibited the maximum improvement in the tensile,flexural,notched impact,compressive strength and hardness by 28.85%,20.00%,91.66%,21.53%and 35.82%,respectively compared to the pure PLA ***,during the thermogravimetric analysis(TGA),the same 80:20 wt.%biocomposite sample showed a minimum weight loss of 20%at 400℃,among *** morphological study using Field Emission Scanning Electron Microscopy(FE-SEM)revealed that the uniform distribution of cellulose reinforcement in the PLA matrix actively improved the mechanical properties of the biocomposites,especially the optimal 80:20 wt.%***,it was evident that the optimal PLA/cellulose biocomposite sample could be a suitable and alternative sustainable,environmentally friendly and biodegradable material for semi/structural applications,replacing synthetic and traditional components.

关键词： Polylactic acid micro crystalline cellulose biocomposite characterizations FESEM environmental pollution

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Improving the operational stability of perovskite solar cells with cesium-doped graphene oxide interlayer

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Journal of Energy Chemistry 2024年第9期96卷 483-490页

作者： Masaud Almalki Katerina Anagnostou Konstantinos Rogdakis Felix T.Eickemeyer Mostafa othman Minas M.Stylianakis Dimitris Tsikritzis Anwar Q.Alanazi Nikolaos Tzoganakis Lukas Pfeifer Rita Therisod Xiaoliang Mo Christian M.Wolff Aïcha Hessler-Wyser shaik M.Zakeeruddin Hong Zhang Emmanuel Kymakis Michael Grätzel Laboratory of Photonics and Interfaces École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Department of Electrical and Computer Engineering Hellenic Mediterranean UniversityHeraklionCrete 71410Greece Institute of Electrical and Microengineering(IEM)Photovoltaics and Thin-Film Electronics Laboratory(PV-Lab) École Polytechnique Fédérale de Lausanne(EPFL)Neuchâtel 2000Switzerland Department of Nursing Faculty of Health SciencesHellenic Mediterranean UniversityCrete 71410Greece Centre Interdisciplinaire de Microscopie Electronique École Polytechnique Fédérale de Lausanne(EPFL)Lausanne 1015Switzerland Future Energy Technology Institute King Abdulaziz City for Science and Technology(KACST)P.O.Box 6086Riyadh 11442Saudi Arabia State Key Laboratory of Photovoltaic Science and Technology Shanghai Frontiers Science Research Base of Intelligent Optoelectronics and PerceptionInstitute of OptoelectronicsDepartment of Materials ScienceFudan UniversityShanghai 200433China

Perovskite solar cells(PSCs)have made great advances in terms of power conversion efficiency(PCE),yet their subpar stability continues to hinder their *** interface between the perovskite layer and the charge-carrier transporting layers plays a crucial role in undermining the stability of *** this work,we propose a strategy to stabilize high-performance PSCs with PCE over 23%by introducing a cesium-doped graphene oxide(Go-Cs)as an interlayer between the perovskite and hole-transporting *** Go-Cs treated PSCs exhibit excellent operational stability with a projected T80(the time where the device PCE reduces to 80%of its initial value)of 2143 h of operation at the maximum powering point under one sun illumination.

关键词： Perovskite solar cells Doped graphene oxide Graphene related material Long-term operational stability

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In situ deposited cobalt-magnesium selenates as an advanced electrode for electrochemical energy storage

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Journal of Magnesium and Alloys 2022年第12期10卷 3565-3575页

作者： Manchi Nagaraju S.Chandra Sekhar Bhimanaboina Ramulu shaik Junied Arbaz Jae Su Yu Department of Electronics and Information Convergence Engineering Kyung Hee University1732 Deogyeong-aeroGihung-guYongin-siGyeonggi-do 17104Republic of Korea

Currently,bimetallic selenates have attracted much attention as a prominent electrode composite material for supercapacitors owing to their higher redox chemistry and superior electrical ***,we synthesized cobalt-magnesium selenates (CoSeo3-Mg Se o4,CMS) via a facile hydrothermal process,followed by *** first,cobalt-magnesium oxide (Co2.32Mg0.68o4,CMo) was in situ prepared by a one-pot hydrothermal *** investigation on the morphological change was performed by synthesizing the same CMo samples at different growth times by keeping the temperature *** CMo electrode designed for 8 h of growth time (CMo-8 h)with an attractive morphology showed a higher areal capacity of 101.7μAh cm-2(at 3 m A cm-2) than the other CMo electrodes prepared for 6 and 10 *** exalted performance was achieved by the selenization of the CMo-8 h sample to form the CMS *** 3 m A cm-2,the resulted CMS exhibited nearly three times higher capacity,i.e.,385.4μAh cm-2,than the CMo-8 h ***,an asymmetric cell fabricated with CMS as a positive electrode also revealed good energy storage *** the applied voltage between 0 and 1.5 V,the asymmetric cell demonstrated maximum energy density of 0.159 m Wh cm-2(18.6 Wh kg-1) and maximum power density of 18.47 m W cm-2(1938 W kg-1),***,novel magnesium-based metal selenates can act as an efficient electrode for energy storage.

关键词： Co2.32Mg0.68o4 CoSeo3–MgSeo4 In situ formation Energy storage Asymmetric cell

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A novel polyherbal formulation containing thymoquinone attenuates carbon tetrachloride-induced hepatorenal injury in a rat model

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Asian Pacific Journal of Tropical Biomedicine 2020年第4期10卷 147-155页

作者： Aftab Ahmad Mohammed F.Abuzinadah Huda M.Alkreathy Hussam I.Kutbi Noor Ahmad shaik Varish Ahmad Shakir Saleem Asif Husain Health Information Technology Department Faculty of Applied StudiesKing Abdulaziz UniversityJeddah-21589Kingdom of Saudi Arabia Department of Medical Laboratory Technology Faculty of Applied Medical SciencesKing Abdulaziz UniversityP.O.Box 80402 Jeddah-21589JeddahKingdom of Saudi Arabia Department of Pharmacology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Pharmacy Practice Faculty of PharmacyKing Abdulaziz UniversityJeddahSaudi Arabia Department of Genetic Medicine Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Public Health College of Health SciencesSaudi Electronic UniversityRiyadhKingdom of Saudi Arabia Department of Pharmaceutical Chemistry School of Pharmaceutical Education and ResearchJamia Hamdard(Hamdard University)New Delhi110062India

objective:To evaluate a novel polyherbal formulation(BSVT)containing the standardized extracts from the leaves of Boerhavia diffusa,Solidago virgaurea,Vitex negundo,and thymoquinone in CCl4 induced hepatorenal toxicity in ***:A total of 36 rats were divided into six groups including normal control,CCl4(2 mL/kg,i.p.),CCl4(2 mL/kg,i.p.)+Cystone?(750 mg/kg p.o.),CCl4(2 mL/kg,i.p.)+BSVT(25 mg/kg,p.o.),CCl4(2 mL/kg,i.p.)+BSVT(50 mg/kg,p.o.),and CCl4(2 mL/kg,i.p.)+BSVT(100 mg/kg,p.o.).All treatments were given for four *** levels of aspartate transaminase,alanine transaminase,alkaline phosphatase,cholesterol,total protein,serum urea,blood urea nitrogen and creatinine were *** dismutase,malondialdehyde,and glutathione peroxidase were evaluated in tissue *** histopathological study of liver and kidney tissues was also ***:Aspartate transaminase,alanine transaminase,alkaline phosphatase,cholesterol,serum urea,blood urea nitrogen and creatinine were significantly elevated(Potal protein was considerably reduced in the CCl4 group as compared to the normal control(Porenal *** addition,superoxide dismutase and glutathione peroxidase activities were significantly decreased(Pondialdehyde levels were increased markedly(Pormulation recovered these parameters towards a normal level in a dose-dependent ***:BSVT formulation ameliorates the hepatorenal toxicity in a dose-dependent ***,clinical studies are required to confirm its efficacy.

关键词： Boerhavia diffusa Solidago virgaurea Vitex negundo Thymoquinone Cystone^(█) Carbon tetrachloride Hepatorenal

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Multiple Low-Lying States for Compound I of P450cam and Chloroperoxidase Revealed from Multireference Ab Initio QM/MM Calculations

Multiple Low-Lying States for Compound I of P450cam and Chlo...

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中国化学会第27届学术年会

作者：宋金帅 Sason shaik The Institute of Chemistry and the Lise Meitner-Minerva Center for Computational Quantum Chemistry Hebrew University of Jerusalem Givat Ram Campus State Key Laboratory of Physical Chemistry and Chemical Engineering Xiamen University

In this work we employed the multireference ab initio CASPT2/MM approach to systematically study the ground and low-lying excited states of important oxoiron（IV）-porphyrin cation-radical active species Por·+FeIV=o（Cys） in P450cam and CPo, termed Compound I （Cpd I）. our calculations suggest that except the ferryl triradicaloid state revealed by previous DFT/MM calculations, the ferryl pentaradicaloid quartet state and perferryl FeV doublet and quartet states may be accessible in energy. This result then raises an intriguing question whether those states contribute to the reactivity of Cpd I. In previous work employing DFT/MM approach, it has been found that hydrogen abstraction barrier with ferryl pentaradicaloid quartet state is lower than that with the ferryl triradicaloid state （Altun, A.;shaik, S.;Thiel, W. J. Am. Chem. Soc. 2007, 129, 8978-8987）. Hence definitely further study is required to explore the reactivity of these states at the multireference ab initio QM/MM level. The experiences and insight gained in this study for high valent transition metal state like FeV could be helpful for future successful application of this type of method to other related systems.

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Association between preterm birth risk and polymorphism and expression of the DNA repair genes oGG1 and APE1 in Saudi women

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BIoCELL 2018年第1期42卷 1-6页

作者： ARWA oSAMA NEMER MoHAMMAD SAUD AL ANAZI RAMESA SHAFI BHAT ARJUMAND S.WARSY ZENEB A BABAY MoHAMMAD HADDAR JILANI shaik SooAD AL-DAIHAN Department of Biochemistry College of Science Genome Research Chair Department of BiochemistryCollege of Science Central Laboratory Female Center for Medical Studies and Scientific Section Department of obs/Gyn College of MedicineKing Saud UniversityP.O.Box 22452Zip code 11495RiyadhSaudi Arabia

Genomic instability and mutations caused by increases in oxidative stress during pregnancy can damage the fetoplacental unit and can upshot preterm *** damage to DNA may possibly be involved in etiology of preterm birth(PTB)which can be repaired by DNA repair *** the present study,we assessed the association of base excision repair gene family by analyzing the association of single nucleotide polymorphisms and genes expression in 8-oxoguanine glycosylase-1(oGG1)and apurinic-apyrimidinic endonuclease 1(APE1)genes with risk of preterm birth in Saudi *** analyzed genotypes of four single nucleotide polymorphisms(SNPs)(rs1052133,rs293795,rs2072668 and rs2075747)in oGG1 gene and three SNPs(rs1130409,rs3136814,and rs3136817)in APE1 gene using TaqMan Genotyping assay kits in 50 pairs of preterm cases and individually matched ***,gene expression level was explored by RT-PCR in 10 pairs of preterm placental tissues and individually matched normal placental *** oGG1 SNP,rs1052133(oR=0.497;c2=1.11;p=0.292)and rs2072668(oR=0.408;c2=1.90;p=0.167)and one APE1 SNP rs3136817(oR=0.458;c2=0.40;p=0.527)showed nonsignificant protective effect against PTB *** expression of both genes under study was found lower in the PTB *** and allele frequencies of both gene SNPs did not show any association with the risk of preterm delivery in Saudi women(P˃0.05).However,synthesis and release of oGG1 and APE1 proteins decreased in preterm placental tissues compared to term delivery reflects the probability of being one of the mechanisms leading to preterm birth.

关键词： Preterm birth oGG1 and APE1 genes oxidative stress SNPs

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Processing and Mechanical Properties of Al₂o₃ and Red Mud Particle Reinforced AA6061 Hybrid Composites

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Journal of Minerals and Materials Characterization and Engineering 2016年第2期4卷 135-142页

作者： shaik Mujeeb Quader B. Suryanarayana Murthy Pinninti Ravinder Reddy Department of Production Engineering DCET Hyderabad India Department of Mechanical Engineering GIT GITAM University Visakhapatnam India Department of Mechanical Engineering CBIT Hyderabad India

Aluminum 6061 alloy metal matrix composites (MMCs) reinforced with four different weight fractions of (Al2o3 + red mud) particles up to 10 wt% were fabricated by a vortex method. The effects of reinforcement content on the mechanical properties of the composites such as hardness and tensile strength were investigated. The density measurements showed that the samples contained little porosity, and the amount of porosity in the composites increased with increasing weight fraction of particles. Scanning electron microscopic observations of the microstructures revealed that the dispersion of the particles was uniform with small clusters at some places and porosity. The results showed that the hardness and the tensile strength of the composites increased with increasing weight fraction of particles.

关键词： MMCs Al2o3 Red Mud AA6061 Compocasting

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Perovskite Solar Cells Yielding Reproducible Photovoltage of 1.20 V

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Research 2019年第1期2019卷 1106-1114页

作者： Essa A.Alharbi M.Ibrahim Dar Neha Arora Mohammad Hayal Alotaibi Yahya A.Alzhrani Pankaj Yadav Wolfgang Tress Ahmed Alyamani Abdulrahman Albadri shaik M.Zakeeruddin Michael Grätzel Laboratory of Photonics and Interfaces Institute of Chemical Sciences and EngineeringEcole Polytechnique Federale de LausanneLausanne CH-1015Switzerland National Nanotechnology Research Centre King Abdulaziz City for Science and Technology(KACST)P.O.Box 6086Riyadh 11442Saudi Arabia National Center for Petrochemicals Technology King Abdulaziz City for Science and Technology(KACST)P.O.Box 608611442 RiyadhSaudi Arabia

High photovoltages and power conversion efciencies of perovskite solar cells(PSCs)can be realized by controlling the undesired nonradiative charge carrier ***,we introduce a judicious amount of guanidinium iodide into mixed-cation and mixed-halide perovskite flms to suppress the parasitic charge carrier recombination,which enabled the fabrication of>20%efcient and operationally stable PSCs yielding reproducible photovoltageas high as 1.20 *** introducing guanidinium iodide into the perovskite precursor solution,the bandgap of the resulting absorber material changed minimally;however,the nonradiative recombination diminished considerably as revealed by time-resolved photoluminescence and electroluminescence ***,using capacitance-frequency measurements,we were able to correlate the hysteresis features exhibited by the PSCs with interfacial charge *** study opens up a path to realize new record efciencies for PSCs based on guanidinium iodide doped perovskite flms.

关键词： perovskite iodide absorber

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