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枯草芽孢杆菌YZ-1突变体库构建与产表面活性素优势菌株筛选

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西南农业学报 2024年

作者：万莉何月秋谢鑫欣刘迎龙何鹏搏 munir shahzad 吴毅歆何鹏飞云南农业大学农学与生物技术学院云南农业大学植物保护学院

【目的】拟通过构建高效杀虫枯草芽孢杆菌YZ-1的转座突变体库，从中筛选出高产表面活性素的突变体菌株，为后续YZ-1的表面活性素合成调控机制解析和开发利用提供前期材料。【方法】利用携带转座子TnYLB-1和温敏型复制蛋白的穿梭载体pMar... 详细信息

【目的】拟通过构建高效杀虫枯草芽孢杆菌YZ-1的转座突变体库，从中筛选出高产表面活性素的突变体菌株，为后续YZ-1的表面活性素合成调控机制解析和开发利用提供前期材料。【方法】利用携带转座子TnYLB-1和温敏型复制蛋白的穿梭载体pMarA转化YZ-1，通过50 ℃温度诱导转座子转座、抗性培养复筛以及特异性PCR扩增验证，构建YZ-1菌株的转座突变体库。组合使用血平板法初筛、溴百里香酚蓝比色法复筛及HPLC定量检测，从突变体库中筛选表面活性素合成显著增强的突变体。【结果】通过自然转化成功地将pMarA质粒导入枯草芽孢杆菌YZ-1中，获得含有3000株转座突变子的YZ-1突变体库，从中筛选出11株表面活性素合成增强的候选菌株。HPLC检测显示与YZ-1野生型相比，表面活性素合成增强和相似的突变体有5株（M1、M6、M7、M8及M9），显著下降有6株（M2、M3、M4、M5、M10及M11），其中表面活性素产量最高的M8突变体为282.4 mg/L较野生型增加了13.7%，而最低的M2菌株为47.53 mg/L，较野生型下降了80.9%。【结论】增设抗性复筛培养可提高YZ-1突变体库的正确率。基于血平板培养和溴百里香酚蓝比色的高通量筛选与HPLC定量检测的YZ-1表面活性素增强型突变体筛选结果不理想，还需进一步改进。

关键词：枯草芽孢杆菌表面活性素 TnYLB-1转座子 pMarA质粒 Himar1转座元件

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高山杜鹃褐斑病病原学研究

菌物学报 2023年第3期42卷 707-718页

作者：寸海春何鹏搏何鹏飞吴毅歆 shahzad munir 汤再祥何月秋云南农业大学植物保护学院云南昆明650201 云南海达新花景园艺种苗有限公司云南昆明605233

为明确引起高山杜鹃褐斑病的病原菌及其生物学特性,采用形态学及分子生物学方法对病原菌进行了鉴定,并采用平板培养法研究了该病菌的生物学特性,筛选了化学杀菌剂及生防细菌。形态特征及rDNA-ITS序列、β-tubulin基因和tef1基因序列分... 详细信息

为明确引起高山杜鹃褐斑病的病原菌及其生物学特性,采用形态学及分子生物学方法对病原菌进行了鉴定,并采用平板培养法研究了该病菌的生物学特性,筛选了化学杀菌剂及生防细菌。形态特征及rDNA-ITS序列、β-tubulin基因和tef1基因序列分析结果表明,引起高山杜鹃褐斑病的病原菌为棒孢新拟盘多毛孢Neopestalotiopsis clavispora;该病原菌最适生长温度为25–28℃,适宜在偏酸性培养基上生长,最适碳、氮源分别为葡萄糖和牛肉膏,最适生长培养基为PDA。毒力测定结果表明,苯醚甲环唑、氟硅唑、氟环唑、戊唑醇及异菌脲对棒孢新拟盘多毛孢的EC50分别为0.7991、2.2756、2.7425、3.7366和4.8757 mg/L;实验室前期筛选出芽胞杆菌属的4株生防细菌,在室内处理离体叶10 d后,对褐斑病的防效达57.51%–75.97%。为研究高山杜鹃褐斑病的防控提供了科学依据。

关键词：高山杜鹃褐斑病棒孢新拟盘多毛孢杀菌剂生防菌

Metal-organic framework-based single-atom electro-/ photocatalysts: Synthesis, energy applications, and opportunities

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Carbon Energy 2024年第1期6卷 1-43页

作者： munir Ahmad Jiahui Chen Jianwen Liu Yan Zhang Zhongxin Song shahzad Afzal Waseem Raza Liaqat Zeb Andleeb Mehmood Arshad Hussain Jiujun Zhang Xian-Zhu Fu Jing-Li Luo Shenzhen Key Laboratory of Energy Electrocatalytic Materials Guangdong Research Center for Interfacial Engineering of Functional MaterialsCollege of Materials Science and EngineeringShenzhen UniversityShenzhenGuangdongChina College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhenGuangdongChina College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhenGuangdongChina Department of Environmental Engineering China Jiliang UniversityHangzhouZhejiangChina Institute for Advanced Study Shenzhen UniversityShenzhenGuangdongChina School of Bioengineering Dalian University of TechnologyDalianLiaoningChina College of Materials Science and Engineering Fuzhou UniversityFuzhouFujianChina Institute for Sustainable Energy Shanghai UniversityShanghaiChina

Single-atom catalysts(SACs)have gained substantial attention because of their exceptional catalytic ***,the high surface energy limits their synthesis,thus creating significant challenges for further *** the last few years,metal–organic frameworks(MOFs)have received significant consideration as ideal candidates for synthesizing SACs due to their tailorable chemistry,tunable morphologies,high porosity,and chemical/thermal *** this perspective,this review thoroughly summarizes the previously reported methods and possible future approaches for constructing MOF-based(MOF-derived-supported and MOF-supported)***,MOF-based SAC's identification techniques are briefly assessed to understand their coordination environments,local electronic structures,spatial distributions,and catalytic/electrochemical reaction *** review systematically highlights several photocatalytic and electrocatalytic applications of MOF-based SACs for energy conversion and storage,including hydrogen evolution reactions,oxygen evolution reactions,O_(2)/CO_(2)/N_(2) reduction reactions,fuel cells,and rechargeable *** light is also shed on the future development of this highly exciting field by highlighting the advantages and limitations of MOF-based SACs.

关键词： carbon energy generation MOF-derived-supported MOF-supported single atoms

Crop diversity and pest management in sustainable agriculture

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Journal of Integrative Agriculture 2019年第9期18卷 1945-1952页

作者： HE Han-ming LIU Li-na shahzad munir Nawaz Haider Bashir WANG Yi YANG Jing LI Cheng-yun State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan/Faculty of Plant Protection Yunnan Agricultural University

Large-scale crop monocultures facilitate the proliferation and increasing prevalence of diseases and pest *** studies highlight the impacts of plant diversification upon pathogens,and the population dynamics of insects and beneficial organism in agricultural *** studies provide evidence that habitat manipulation techniques such as intercropping,relay,and rotation can significantly improve disease and pest *** review introduces the concept of crop diversity,considers recent insights and mechanisms underlying crop diversity,and discusses its potential for improving sustainable agricultural ***,the phytobiomes resulting from increased crop diversity are increasingly recognized for their contribution to disease and pest ***,understanding the interactions between pathogens or pests with their host phytobiome may lead to novel options for the prevention of *** advances in the agricultural systems include:(i)a better understanding of the mechanisms of interactions between crop species and genotypes;(ii)ecological progress including a better understanding of the context-dependency of those interactions;and(iii)the role of microtopographic variation in agricultural systems for priming basal resistance to multiple pests and pathogens by intercropped *** also highlight recent progress in China and the potential options for habitat management and design that enhance the ecological role of biodiversity in agroecosystems.

关键词： crop diversity pattern pest and disease management priming resistance phytobiome holobiont sustainability

(Sm/Eu/Tm)^(3+) doped tantalum semiconductor system for photovoltaic and electrochemical functionality amplification

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Journal of Rare Earths 2024年第8期42卷 1586-1594,I0007页

作者： Shaan Bibi Jaffri Khuram shahzad Ahmad Isaac Abrahams Ibrahim A.A Materials and Environmental Chemistry Lab Lab-E21Department of Environmental SciencesFatima Jinnah Women UniversityRawalpindiPakistan School of Physical and Chemical Sciences Queen Mary University of LondonLondonUnited Kingdom Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box 2455Riyadh11451Saudi Arabia

This work reports the synthesis,characterization,and energy focused applications of the novel lanthanides co-doped tantalum pentoxide hetero-system(Sm^(3+)-Eu^(3+)-Tm^(3+):Ta_(2)O_(5)).Ln^(3+)-doped Ta_(2)O_(5) express excellent opto-electronic features reflected by the narrow band gap energy of 3.87 *** vibrations confirm the presence of Ta-O-Ta and Ta-O *** synthesized system possesses orthorhombic geometry with 59.46 nm particle *** the smoother and compact morphology,the synthesized material succeeds in augmenting the performance of different systems aimed at energy *** ambient perovskite solar cell device fabricated with the Ln^(3+)-doped Ta_(2)O_(5) as an electron transport layer excels in achieving an efficiency and fill factor of 14.17% and 76% under artificial *** device was marked by the negligible hysteresis behavior showing profound photovoltaic *** electrochemical activity of the Ln^(3+)-doped Ta_(2)O_(5) decorated electrode was evaluated for electrical charge storage potential with pseudocapacitive behavior,With the highest specific capacitance of 355.39 F/g and quicker ionic diffusion rate,the designed electrode excels conventionally used ***-catalysis of water with Ln^(3+)-doped Ta_(2)O_(5) material indicates its capacity for H_(2) production with the lowest overpotential and Tafel slope values of 148 and 121.2 mV/dec,while the O_(2) generation is comparatively *** the stable electrochemical output,this rare earth modified material has the potential to replace conventionally used environmentally perilous and costly materials.

关键词： Lanthanide doping Rare earth materials Perovskite solar cells Supercapacitors Water splitting

Improved Proximal Policy Optimization Algorithm for Sequential Security-constrained Optimal Power Flow Based on Expert Knowledge and Safety Layer

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 742-753页

作者： Yanbo Chen Qintao Du Honghai Liu Liangcheng Cheng Muhammad shahzad Younis State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources and School of Electrical&Electronic Engineering North China Electric Power UniversityBeijing 102206China School of Engineering Xining UniversityXining 810008Chinaand Key Laboratory of Smart Operation of New Energy Power SystemMinistry of EducationQinghai UniversityXining 810016China China Electric Power Research Institute Nanjing 210003China National University of Sciences and Technology Islamabad44000Pakistan

In recent years,reinforcement learning(RL)has emerged as a solution for model-free dynamic programming problem that cannot be effectively solved by traditional optimization *** has gradually been applied in the fields such as economic dispatch of power systems due to its strong selflearning and self-optimizing ***,existing economic scheduling methods based on RL ignore security risks that the agent may bring during exploration,which poses a risk of issuing instructions that threaten the safe operation of power ***,we propose an improved proximal policy optimization algorithm for sequential security-constrained optimal power flow(SCOPF)based on expert knowledge and safety layer to determine active power dispatch strategy,voltage optimization scheme of the units,and charging/discharging dispatch of energy storage *** expert experience is introduced to improve the ability to enforce constraints such as power balance in training process while guiding agent to effectively improve the utilization rate of renewable ***,to avoid line overload,we add a safety layer at the end of the policy network by introducing transmission constraints to avoid dangerous actions and tackle sequential SCOPF *** results on an improved IEEE 118-bus system verify the effectiveness of the proposed algorithm.

关键词： Sequential security-constrained optimal power flow(SCOPF) expert experience safety layer renewable energy safe reinforcement learning

10种农业化学品对柑橘内生菌枯草芽孢杆菌L1-21的影响

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生物安全学报 2022年第1期31卷 34-40页

作者：李咏梅蒋佳容 shahzad munir 何鹏飞吴毅歆谢金思潘祖贤何鹏搏何月秋云南农业大学植物保护学院云南昆明650201 云南农业大学农学与生物技术学院云南昆明650201

【目的】明确农业化学品对柑橘内生菌的影响,指导柑橘合理用药。【方法】采用平板菌落计数法,测定了6种杀虫剂、3种杀菌剂和微肥EDTA-Cu对生防菌株枯草芽孢杆菌L1-21的相容性及对柑橘植株内生菌的抑制作用。【结果】56.00 mg·L^(-1)吡... 详细信息

【目的】明确农业化学品对柑橘内生菌的影响,指导柑橘合理用药。【方法】采用平板菌落计数法,测定了6种杀虫剂、3种杀菌剂和微肥EDTA-Cu对生防菌株枯草芽孢杆菌L1-21的相容性及对柑橘植株内生菌的抑制作用。【结果】56.00 mg·L^(-1)吡虫啉、121.80 mg·L^(-1)虫螨腈、4.00 mg·L^(-1)甲氨基阿维菌素、50.00 mg·L^(-1)噻虫嗪、45.60 mg·L^(-1)氟氯氰菊酯、800.00 mg·L^(-1)辛硫磷、402.00 mg·L^(-1)喹啉铜、3728.00 mg·L^(-1)硫磺、620.60 mg·L^(-1)氧化亚铜和321.00 mg·L^(-1)EDTA-Cu在平板上对L1-21的抑制率均达100.00%。2倍剂量处理离体叶片24 h后,除8.00 mg·L^(-1)甲氨基阿维菌素的抑制率(16.84%)显著低于其他5种杀虫剂外,其他5种杀虫剂对L1-21的抑制率均无显著差异。2倍剂量处理柑橘活体叶片24 h后,8.00 mg·L^(-1)甲氨基阿维菌素对柑橘叶片内的L1-21抑制作用(4.23%)不明显,显著低于其他5种杀虫剂和3种杀菌剂与微肥EDTACu;对内生菌总量的抑制率以8.00 mg·L^(-1)甲氨基阿维菌素(6.78%)最低,其他制品达46.90%~70.06%,且差异未达显著水平。【结论】10种农业化学品在平板上均可抑制L1-21,抑制率的大小与其浓度有关。2倍田间登记使用量处理时,10种农业化学品均可影响L1-21在柑橘叶片上的定殖及降低柑橘内生细菌总量。因此,田间施用化学品对柑橘内生菌的负面影响不可忽视。

关键词：吡虫啉喹啉铜柑橘内生菌硫磺枯草芽孢杆菌L1-21

Clinical applications of stem cell-derived exosomes

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Signal Transduction and Targeted Therapy 2024年第2期9卷 621-651页

作者： Fei Tan Xuran Li Zhao Wang Jiaojiao Li Khawar shahzad Jialin Zheng Department of ORL-HNS Shanghai Fourth People’s Hospitaland School of MedicineTongji UniversityShanghaiChina Plasma Medicine and Surgical Implants Center Tongji UniversityShanghaiChina The Royal College of Surgeons in Ireland DublinIreland The Royal College of Surgeons of England LondonUK Center for Translational Neurodegeneration and Regenerative Therapy Tongji Hospital affiliated to Tongji University School of MedicineShanghaiChina Shanghai Frontiers Science Center of Nanocatalytic Medicine Tongji UniversityShanghaiChina

Although stem cell-based therapy has demonstrated considerable potential to manage certain diseases more successfully than conventional surgery,it nevertheless comes with inescapable drawbacks that might limit its clinical *** to stem cells,stem cell-derived exosomes possess numerous advantages,such as non-immunogenicity,non-infusion toxicity,easy access,effortless preservation,and freedom from tumorigenic potential and ethical *** can inherit similar therapeutic effects from their parental cells such as embryonic stem cells and adult stem cells through vertical delivery of their pluripotency or *** a thorough search and meticulous dissection of relevant literature from the last five years,we present this comprehensive,up-to-date,specialty-specific and disease-oriented review to highlight the surgical application and potential of stem cell-derived *** derived from stem cells(e.g.,embryonic,induced pluripotent,hematopoietic,mesenchymal,neural,and endothelial stem cells)are capable of treating numerous diseases encountered in orthopedic surgery,neurosurgery,plastic surgery,general surgery,cardiothoracic surgery,urology,head and neck surgery,ophthalmology,and obstetrics and *** diverse therapeutic effects of stem cells-derived exosomes are a hierarchical translation through tissue-specific responses,and cell-specific molecular signaling *** this review,we highlight stem cell-derived exosomes as a viable and potent alternative to stem cell-based therapy in managing various surgical *** recommend that future research combines wisdoms from surgeons,nanomedicine practitioners,and stem cell researchers in this relevant and intriguing research area.

关键词： viable stem capable

Multi-Elemental Chemostratigraphy,Sequence Development,Depositional History,and Environmental Importance of Early Eocene Red Beds(Kuldana Formation)in NW Himalayas,Pakistan

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Journal of Earth Science 2024年第2期35卷 349-375页

作者： Amir shahzad George Kontakiotis Thierry Adatte Khawaja Shoaib Ahmed Muhammad Tayyib Riaz Hammad Tariq Janjuhah Evangelia Besiou Institute of Geology University of Azad Jammu and KashmirMuzaffarabad13100Pakistan Department of Historical Geology-Paleontology Faculty of Geology and GeoenvironmentSchool of Earth SciencesNational and Kapodistrian University of AthensPanepistimiopolisZografou15784Greece Institute of Earth Sciences(ISTE) University of LausanneLausanneCH-1015Switzerland Department of Geology Shaheed Benazir Bhutto UniversitySheringalPeshawar18050Pakistan

The Eocene Kuldana Formation(KF)in the Yadgar area of Pakistan,comprises a diverse range of sedimentary facies,including variegated red beds of shales,mudstones,and sandstones,as well as interbedded limestone and *** this study,we conducted an integrated micropaleontological,sedimentological,mineralogical,and geochemical investigation to determine the depositional setting,biochronology,provenance,and paleoclimate of the *** study identified six lithofacies and six microfacies,which indicate a variety of environments ranging from floodplains and channels to the margins and shallow marine *** nannofossil biostratigraphy places the KF in the Early Eocene,more precisely the NP10 zone(Ypresian),and the fossil zone of benthic foraminifera classifies the study section as the Shallow Benthic Zone SBZ-8(Middle Ilerdian 2).In terms of petrography,the KF sandstone was classified as litharenite and feldspathic litharenite,while the QtFL diagram suggests a recycled *** proxies indicate an oxidizing environment,a high-to-low regular sedimentation rate,moderate-to-intense chemical weathering in the source region,and a warm-humid to dry climate during the deposition of ***,the findings suggest that the deposition of KF marks the end of Neo-Tethys due to the Early Eocene Indian–Kohistan collision and that the uplifting of the Himalayas provided the source for the deposition of KF in the foreland *** study provides new insights into the depositional environment,biochronology,provenance,and paleoclimate of KF,and highlights the potential for red beds as reliable indicators of oxygenation levels in proximity to mineral deposits.

关键词： sedimentary microfacies Paleocene–Eocene thermal maximum stratigraphic correlations paleoenvironmental reconstruction nannofossils paleontology