检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

高山杜鹃褐斑病病原学研究

菌物学报 2023年第3期42卷 707-718页

作者：寸海春何鹏搏何鹏飞吴毅歆 shahzad munir 汤再祥何月秋云南农业大学植物保护学院云南昆明650201 云南海达新花景园艺种苗有限公司云南昆明605233

为明确引起高山杜鹃褐斑病的病原菌及其生物学特性,采用形态学及分子生物学方法对病原菌进行了鉴定,并采用平板培养法研究了该病菌的生物学特性,筛选了化学杀菌剂及生防细菌。形态特征及rDNA-ITS序列、β-tubulin基因和tef1基因序列分... 详细信息

为明确引起高山杜鹃褐斑病的病原菌及其生物学特性,采用形态学及分子生物学方法对病原菌进行了鉴定,并采用平板培养法研究了该病菌的生物学特性,筛选了化学杀菌剂及生防细菌。形态特征及rDNA-ITS序列、β-tubulin基因和tef1基因序列分析结果表明,引起高山杜鹃褐斑病的病原菌为棒孢新拟盘多毛孢Neopestalotiopsis clavispora;该病原菌最适生长温度为25–28℃,适宜在偏酸性培养基上生长,最适碳、氮源分别为葡萄糖和牛肉膏,最适生长培养基为PDA。毒力测定结果表明,苯醚甲环唑、氟硅唑、氟环唑、戊唑醇及异菌脲对棒孢新拟盘多毛孢的EC50分别为0.7991、2.2756、2.7425、3.7366和4.8757 mg/L;实验室前期筛选出芽胞杆菌属的4株生防细菌,在室内处理离体叶10 d后,对褐斑病的防效达57.51%–75.97%。为研究高山杜鹃褐斑病的防控提供了科学依据。

关键词：高山杜鹃褐斑病棒孢新拟盘多毛孢杀菌剂生防菌

First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 785-788页

作者： Mubbashir Hussain shahzad munir Sultan Ayaz Bahar Ullah Khattak Taj Ali Khan Niaz Muhammad Muhammad Anees Hazir Rahman Muhammad Qasim Muhammad Ameen Jamal Irfan Ahmed Kashif Rahim Humaira Mazhar Noha Watanay Mohamed Kasbari Vector Borne Diseases Laboratory Department of MicrobiologyKohat University of Science and Technology Kohat Department of Plant Pathology Faculty of Plant ProtectionYunnan Agricultural University College of Animal Husbandry and Veterinary Sciences Abdul Wali Khan University Mardan College of Animal Science and Technology Yunnan Agricultural University Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and BiotechnologyCollege of Life SciencesBeijing Normal University US Naval Medical Research Unit-3 (NAMRU 3) ANSES Animal Health Laboratory

Objective: To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis(CL) is endemic and was thought to be caused by Leishmania tropica only. Methods: Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results: Leishmania amastigotes were detected by microscopy in 308 of 432 samples(71.3%) while 374 out of 432 samples(86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed Leishmania tropica in 351 and Leishmania major in 6 biopsy samples. Conclusions: This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of Leishmania major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

关键词： Cutaneous leishmaniasis ITS-PCR RFLP Leishmania tropica Khyber Pakhtunkhwa

Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain shahzad munir Abdullah Jalal Taj Ali Khan Niaz Muhammad Bahar Ullah Khattak Abdullah Khan Irfan Ahmed Zulqarnain Baloch Nawaz Haider Bashir Muhammad Ameen Jamal Kashif Rahim Humaira Mazhar Maira Riaz Noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

地毯草叶斑病病原菌鉴定和寄主范围测定

草业科学 2024年第11期41卷 2604-2611页

作者：李能萍何鹏搏刘迎龙何鹏飞吴毅歆唐萍王再强 munir shahzad 孔宝华何月秋云南农业大学省部共建云南生物资源保护与利用国家重点实验室云南昆明650201

2022年,在昆明市松华坝蔬菜基地发现大片地毯草(Axonopus compressus)上发生1种由未知病原菌引起的地毯草叶部病害,其病斑呈褐色近圆形或长条形。为明确其致病菌,并对该病原菌进行寄主范围测定和杀菌剂毒力测定。通过分离培养、形态学... 详细信息

2022年,在昆明市松华坝蔬菜基地发现大片地毯草(Axonopus compressus)上发生1种由未知病原菌引起的地毯草叶部病害,其病斑呈褐色近圆形或长条形。为明确其致病菌,并对该病原菌进行寄主范围测定和杀菌剂毒力测定。通过分离培养、形态学和多基因联合测序分析,结果表明,引起地毯草叶斑病的病原菌为玉米平脐蠕孢(Bipolaris zeae)。孢子液离体叶片接种21种经济作物及常见杂草,发现***还可侵染水稻(Oryza sativa)、玉米(Zea mays)、高粱(Sorghum bicolor)、小麦(Triticum aestivum)、薏苡(Coix lacryma-jobi)、棕叶狗尾草(Setaria palmifolia)、狗尾草(Setaria viridis)、野燕麦(Avena fatua)、黑麦草(Lolium perenne)、稗草(Echinochloa crusgalli)。7种杀菌剂对***毒力表现为苯醚甲环唑EC50最小(0.381 mg∙L^(-1)),其他毒力(EC50)由大到小依次为氟唑菌酰胺(0.988 mg∙L^(-1))、异菌脲(1.864 mg∙L^(-1))、代森锰锌(5.401 mg∙L^(-1))、甲基硫菌灵(930.572 mg∙L^(-1))、嘧菌酯(989.571 mg∙L^(-1))、百菌清(1547.315 mg∙L^(-1))。本研究明确了引起地毯草叶斑病的病原菌及其寄主范围,并筛选出了抑菌效果较好的杀菌剂,为该病害的诊断与防控提供了理论依据。

关键词：地毯草叶斑病病原鉴定玉米平脐蠕孢致病力苯醚甲环唑毒力测定

表面活性素促进枯草芽胞杆菌XF-1在大白菜叶际定殖能力研究

在线全文

学校读者我要写书评

暂无评论

植物保护 2021年第5期47卷 28-34页

作者：何朋杰崔文艳何鹏飞李兴玉吴毅歆 shahzad munir 何月秋省部共建云南生物资源保护与利用国家重点实验室云南农业大学昆明650201 贵州中医药大学基础医学院贵阳550025

枯草芽胞杆菌XF-1是一株大白菜内生生防菌,通过叶面喷施的方式可有效防控大白菜根肿病,但其叶际定殖的分子机制尚不明确。为了明确表面活性素对XF-1在大白菜叶际定殖的影响,进一步提高其在田间应用效果,本研究通过测定生长曲线、泳动运... 详细信息

枯草芽胞杆菌XF-1是一株大白菜内生生防菌,通过叶面喷施的方式可有效防控大白菜根肿病,但其叶际定殖的分子机制尚不明确。为了明确表面活性素对XF-1在大白菜叶际定殖的影响,进一步提高其在田间应用效果,本研究通过测定生长曲线、泳动运动、群集运动和24孔细胞培养板静置培养试验,分析表面活性素对XF-1菌株生长速率、运动能力和生物膜形成水平的影响;利用叶片-微生物互作分析和定殖试验,测定XF-1及突变体XF-1-ΔsrfA在大白菜叶表黏附和植株内的定殖能力。结果表明,XF-1-ΔsrfA与XF-1相比生长曲线没有明显差异,泳动能力和群集运动能力分别显著下降了36.8%和43.9%,静置培养24 h后生物膜形成能力显著下降53.9%,48 h后无显著差异。叶片-微生物互作试验中,突变体标记菌XF-1-ΔsrfA-gfp黏附效率较XF-1-gfp约下降80%。喷施接种3~7 d后,XF-1-ΔsrfA-gfp相较于XF-1-gfp在大白菜叶片内和根系中的定殖数量均显著下降。试验结果表明表面活性素对枯草芽胞杆菌XF-1在大白菜叶际定殖具有较为明显的促进作用,srfA基因的缺失显著抑制XF-1的叶际定殖能力。

关键词：表面活性素枯草芽胞杆菌定殖生长曲线群集运动生物膜

Different strategies to properly manage the citrus huanglongbing in China

在线全文

学校读者我要写书评

暂无评论

Different strategies to properly manage the citrus huanglong...

枯草芽孢杆菌L1-21在柑橘上的定殖能力及其防治绿霉病效果

中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

作者： shahzad munir Li Yongmei He Pengfei Wu Yixin He Pengjie Cui Wenyan 何月秋 Faculty of Plant Protection Yunnan Agricultural University

Huanglongbing(HLB) also known as citrus greening is one of the serious diseases spread all over citrus growing regions of the *** causal agent is Candidatus Liberibacter asiaticus,a phytopathogenic bacterium that can infect all Citrus *** causal agents of HLB have been putatively identified,and their transmission pathways and worldwide population structure have been extensively ***,very little isknown about the epidemiologic relationships of ***,which has limited the scope of HLB research and especially the development of control ***-affected plants produce damaged fruits and die within several *** candidate compounds show promise in eliminating or suppressing disease symptoms;however,others need to be evaluated in the form of antibiotics with less ***,thermotherapy can be useful for combating *** application of epibrassinolide reduces the causal agent of HLB and might provide a useful tool within an integrated management *** in oil(W/O) nanoemulsion formulation may provide a useful model for the effective delivery of chemical compounds into citrus phloem via a foliar spray for controlling HLB citrus *** production of transgenic plants with modified volatile profiles that are more tolerant to citrus greening disease can be helpful in reducing the disease ***,the potential impact on HLB should be conducted for discovering novel antibacterial *** research is needed to provide an eco-friendly control strategy for HLB in the form of ***,unfortunately,no study has reported the successful control of the disease by microbial control agents *** HLBresistant citrus varieties are being developed,it will presumably take many years before they are ***,alternative measures are being used to reduce the incidence of this disease or HLB pathogens in the *** far,there are no curative methods to control th

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

华中农业大学学报 2021年第5期40卷 31-36页

作者：李健何鹏飞李咏梅吴毅歆何鹏搏孔宝华李兴玉 munir shahzad 何月秋云南农业大学植物保护学院昆明650201 云南农业大学农学与生物技术学院昆明650201

为明确枯草芽孢杆菌L1-21在柑橘叶片、果实内的定殖能力及其防治果实绿霉病的效果,通过向柑橘叶片喷施10^(6)cfu/mL的L1-21菌株的绿色荧光标记菌(L1-21-GFP)发酵液,测定其在叶片内的定殖数量及传导能力;采用10^(8)cfu/mL的菌液喷施和浸... 详细信息

为明确枯草芽孢杆菌L1-21在柑橘叶片、果实内的定殖能力及其防治果实绿霉病的效果,通过向柑橘叶片喷施10^(6)cfu/mL的L1-21菌株的绿色荧光标记菌(L1-21-GFP)发酵液,测定其在叶片内的定殖数量及传导能力;采用10^(8)cfu/mL的菌液喷施和浸泡处理柑橘果实,检测其在果实内的定殖情况,用注射法接种Penicillium digitatum孢子并计算发病率,测定其对绿霉病的防控效果。结果显示,处理后10 min,L1-21-GFP在叶表及叶肉中的定殖量分别达到1.36×10^(3)和8.08×10^(2)cfu/cm^(2),并呈现持续增长的趋势。处理后1 h,L1-21-GFP向下传导到叶表的菌含量为1.51×10^(2)cfu/cm^(2),并稳定存在于叶表中;处理后2 h,传导到叶肉的菌含量为5.67 cfu/cm^(2),逐渐增加到30 h时的1.15×10^(3)cfu/cm^(2)。L1-21-GFP能在果实内定殖,浸泡1 h果肉和果皮中的定殖量分别为9.51×10^(3)和3.51×10^(4)cfu/g,但在不同柑橘品种果实间定殖能力有显著性差异,椪柑中定殖量最高。浸泡30 min后,L1-21对果实绿霉病防效第3天为100%,第5天为80.36%。以上结果表明,枯草芽孢杆菌L1-21能高效定殖于柑橘叶片及果实内,可以利用该菌株防治柑橘病害。

关键词：枯草芽孢杆菌内生菌定殖防效绿霉病柑橘

10种农业化学品对柑橘内生菌枯草芽孢杆菌L1-21的影响

在线全文

学校读者我要写书评

暂无评论

生物安全学报 2022年第1期31卷 34-40页

作者：李咏梅蒋佳容 shahzad munir 何鹏飞吴毅歆谢金思潘祖贤何鹏搏何月秋云南农业大学植物保护学院云南昆明650201 云南农业大学农学与生物技术学院云南昆明650201

【目的】明确农业化学品对柑橘内生菌的影响,指导柑橘合理用药。【方法】采用平板菌落计数法,测定了6种杀虫剂、3种杀菌剂和微肥EDTA-Cu对生防菌株枯草芽孢杆菌L1-21的相容性及对柑橘植株内生菌的抑制作用。【结果】56.00 mg·L^(-1)吡... 详细信息

【目的】明确农业化学品对柑橘内生菌的影响,指导柑橘合理用药。【方法】采用平板菌落计数法,测定了6种杀虫剂、3种杀菌剂和微肥EDTA-Cu对生防菌株枯草芽孢杆菌L1-21的相容性及对柑橘植株内生菌的抑制作用。【结果】56.00 mg·L^(-1)吡虫啉、121.80 mg·L^(-1)虫螨腈、4.00 mg·L^(-1)甲氨基阿维菌素、50.00 mg·L^(-1)噻虫嗪、45.60 mg·L^(-1)氟氯氰菊酯、800.00 mg·L^(-1)辛硫磷、402.00 mg·L^(-1)喹啉铜、3728.00 mg·L^(-1)硫磺、620.60 mg·L^(-1)氧化亚铜和321.00 mg·L^(-1)EDTA-Cu在平板上对L1-21的抑制率均达100.00%。2倍剂量处理离体叶片24 h后,除8.00 mg·L^(-1)甲氨基阿维菌素的抑制率(16.84%)显著低于其他5种杀虫剂外,其他5种杀虫剂对L1-21的抑制率均无显著差异。2倍剂量处理柑橘活体叶片24 h后,8.00 mg·L^(-1)甲氨基阿维菌素对柑橘叶片内的L1-21抑制作用(4.23%)不明显,显著低于其他5种杀虫剂和3种杀菌剂与微肥EDTACu;对内生菌总量的抑制率以8.00 mg·L^(-1)甲氨基阿维菌素(6.78%)最低,其他制品达46.90%~70.06%,且差异未达显著水平。【结论】10种农业化学品在平板上均可抑制L1-21,抑制率的大小与其浓度有关。2倍田间登记使用量处理时,10种农业化学品均可影响L1-21在柑橘叶片上的定殖及降低柑橘内生细菌总量。因此,田间施用化学品对柑橘内生菌的负面影响不可忽视。

关键词：吡虫啉喹啉铜柑橘内生菌硫磺枯草芽孢杆菌L1-21

枯草芽孢杆菌YZ-1突变体库构建与产表面活性素优势菌株筛选

在线全文

学校读者我要写书评

暂无评论

西南农业学报 2024年

作者：万莉何月秋谢鑫欣刘迎龙何鹏搏 munir shahzad 吴毅歆何鹏飞云南农业大学农学与生物技术学院云南农业大学植物保护学院

【目的】拟通过构建高效杀虫枯草芽孢杆菌YZ-1的转座突变体库，从中筛选出高产表面活性素的突变体菌株，为后续YZ-1的表面活性素合成调控机制解析和开发利用提供前期材料。【方法】利用携带转座子TnYLB-1和温敏型复制蛋白的穿梭载体pMar... 详细信息

【目的】拟通过构建高效杀虫枯草芽孢杆菌YZ-1的转座突变体库，从中筛选出高产表面活性素的突变体菌株，为后续YZ-1的表面活性素合成调控机制解析和开发利用提供前期材料。【方法】利用携带转座子TnYLB-1和温敏型复制蛋白的穿梭载体pMarA转化YZ-1，通过50 ℃温度诱导转座子转座、抗性培养复筛以及特异性PCR扩增验证，构建YZ-1菌株的转座突变体库。组合使用血平板法初筛、溴百里香酚蓝比色法复筛及HPLC定量检测，从突变体库中筛选表面活性素合成显著增强的突变体。【结果】通过自然转化成功地将pMarA质粒导入枯草芽孢杆菌YZ-1中，获得含有3000株转座突变子的YZ-1突变体库，从中筛选出11株表面活性素合成增强的候选菌株。HPLC检测显示与YZ-1野生型相比，表面活性素合成增强和相似的突变体有5株（M1、M6、M7、M8及M9），显著下降有6株（M2、M3、M4、M5、M10及M11），其中表面活性素产量最高的M8突变体为282.4 mg/L较野生型增加了13.7%，而最低的M2菌株为47.53 mg/L，较野生型下降了80.9%。【结论】增设抗性复筛培养可提高YZ-1突变体库的正确率。基于血平板培养和溴百里香酚蓝比色的高通量筛选与HPLC定量检测的YZ-1表面活性素增强型突变体筛选结果不理想，还需进一步改进。

关键词：枯草芽孢杆菌表面活性素 TnYLB-1转座子 pMarA质粒 Himar1转座元件