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Open Journal of Biophysics 2012年第4期2卷 109-116页

作者： shadi abu-baker Gary A. Lorigan Miami University Department of Chemistry and Biochemistry Oxford USA

Solid-state NMR spectroscopy is routinely used to determine the structural and dynamic properties of both membrane proteins and peptides in phospholipid bilayers [1-26]. From the perspective of the perpetuated lipids, 2H solid-state NMR spectroscopy can be used to probe the effect of embedded proteins on the order and dynamics of the acyl chains of phospholipid bilayers [8-13]. Moreover, 31P solid-state NMR spectroscopy can be used to investigate the interaction of peptides, proteins and drugs with phospholipid head groups [11-14]. The secondary structure of 13C = O site-specific isotopically labeled peptides or proteins inserted into lipid bilayers can be probed utilizing 13C CPMAS solid-state NMR spectroscopy [15-18]. Also, solid-state NMR spectroscopic studies can be utilized to ascertain pertinent informa- tion on the backbone and side-chain dynamics of 2H- and 15N-labeled proteins, respectively, in phospholipid bilayers [19-26]. Finally, specific 15N-labeled amide sites on a protein embedded inside oriented bilayers can be used to probe the alignment of the helices with respect to the bilayer normal [2]. A brief summary of all these solid-state NMR ap- proaches are provided in this minireview.

关键词： Solid-State NMR Structure and Dynamics Membrane Proteins

Improved Solid-Phase Peptide Synthesis of Wild-Type and Phosphorylated Phospholamban Using a Pseudoproline Dipeptide

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Open Journal of Synthesis Theory and Applications 2012年第2期1卷 9-12页

作者： shadi abu-baker Gary A. Lorigan Department of Chemistry and Biochemistry Miami University Oxford USA

In this study, we report that the insertion of a pseudoproline dipeptide for the solid-phase peptide synthesis of wild-type Phospholamban protein (WT-PLB) has two important advantages. First, it disrupts the formation of different secondary structures, which is responsible for poor couplings during the preparation of highly aggregated sequences. Second, it enhances the purities and solubility of crude products leading to easier HPLC purification.

关键词： Solid-State Peptide Synthesis Pseudoproline Dipeptide Phospholamban

Fly Ash for Removal of Contaminates from Coal Mine Drainage

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Natural Science 2016年第9期8卷 397-402页

作者： shadi abu-baker Shahrokh Ghaffari Lucy Egdell Kristin Haser College of Arts and Sciences Ohio University Zanesville Zanesville USA

In this study, fly ash obtained from a coal burning power plant was used to remove iron from coal mine drainage as well as raising the pH to acceptable limits for natural water using column chromatography. The results of this study indicate that fly ash has the ability to completely remove iron and reduce acidity of coal mine water drainage. This approach can be extended to include other contaminants such as magnesium.

关键词： Fly Ash Coal Mine Water Contamination Reduction Iron Contamination

Engaging Freshman Undergraduate Students in Faculty Environmental Science Research: Testing the Local Surface Waters for Nitrate, Phosphate, and Ammonium Ions Using Two Affordable Methods as an Example

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Green and Sustainable Chemistry 2016年第3期6卷 143-149页

作者： shadi abu-baker Christian Frazier Nathaniel Frazier Shahrokh Ghaffari College of Arts and Sciences Ohio University Zanesville Zanesville OH USA

Clean water is a crucial factor that contributes to the health of eco-systems and their inhabitants. Testing local surface water is an exciting way to engage freshman undergraduates in research which shows them the importance of protecting their communities and environment. Two affordable methods: a Colorimeter and Ion-Selective Probe were used to analyze nitrate, phosphate and ammonia levels in local surface water samples. The samples were collected from the Muskingum River which runs through Southeastern Ohio (Muskingum County) and were collected at 2 mile increments. We believe that this set of experiments would be beneficial if implemented into a college freshman level chemistry lab, thereby, promoting self-awareness of water safety, eco-system safety and community involvement. In addition, the students will learn how our environment is effected by our actions when we neglect to properly monitor the type of pollutants we allow into our water ways.

关键词： Nitrate South Eastern Ohio Affordable Muskingum Phosphate Ammonia Environmental Monitoring River Water Toxicity Vernier Electrode PASCO Colorimeter

Effective Method to Resolve the Chromosome Numbers in Pistacia Species (Anacardiaceae)

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American Journal of Plant Sciences 2014年第20期5卷 2913-2916页

作者： Mohannad Al-Saghir shadi abu baker Robert Pusok Department of Environmental and Plant Biology Ohio University Zanesville Zanesville OH USA Department of Chemistry Ohio University Zanesville Zanesville OH USA

The genus Pistacia L. belongs to the Anacardiaceae family and includes at least eleven species. Cytogenetic studies addressing the genus Pistacia are rather few. Chromosome numbers of the different Pistacia species, revealed by these studies, are questionable due to the fact that poor chromosome counting protocols were used, and these protocols are hampered by the extremely small-sized chromosomes of Pistacia species. The aim of this study was to develop a more effective method to resolve the chromosome numbers in Pistacia species using a fluorescent microscope. The method described here is modified from the Sigma Plant Protoplast Digest/Wash Solution protocol. The method used here is highly effective for karyotyping analysis and studying population genetics of Pistacia species. Moreover, it is easy and can be reproduced for other species that have smaller chromosomes. This method can be used for plant herbarium specimens or field plants. This study provides valuable chromosomal data for cytogeneticists and plant breeders who are working on this genus. It provides additional insight into understanding the taxonomic and phylogenetic relationships among Pistacia species. The chromosomes described here are also suitable for gene and genome mapping.

关键词： Pistacia Cytogenetics Chromosome Numbers Fluorescent Microscopy

Cytotoxicity and Selectivity in Skin Cancer by SapC-DOPS Nanovesicles

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Journal of Cancer Therapy 2012年第4期3卷 321-326页

作者： shadi abu-baker Zhengtao Chu Ashley M. Stevens Jie Li Xiaoyang Qi Department of Dermatology University of Miami Miami FL Division of Hematology/Oncology Department of Internal Medicine University of Cincinnati College of Medicine Division of Human Genetics Department of Pediatrics Cincinnati Children’s Hospital Medical Center Cincinnati OH

Squamous cell carcinoma (SCC) and melanoma are malignant human cancers of the skin with an annual mortality that exceeds 10,000 cases every year in the USA alone. In this study, the lysosomal protein saposin C (SapC) and the phospholipid dioloylphosphatidylserine (DOPS) were assembled into cancer-selective nanovesicles (SapC-DOPS) and successfully tested using several in vitro and in vivo skin cancer models. Using MTT assay that measures the percentage of cell death, SapC-DOPS cytotoxic effect on three skin tumor cell lines (squamous cell carcinoma, SK-MEL-28, and MeWo) was compared to two normal nontumorigenic skin cells lines, normal immortalized keratinocyte (NIK) and human fibroblast cell (HFC). We observed that the nanovesicles selectively killed the skin cancer cells by inducing apoptotic cell death whereas untransformed skin cancer cells remained unaffected. Using subcutaneous skin tumor xenografts, animals treated with SapC-DOPS by subcutaneous injection showed a 79.4% by volume tumor reduced compared to the control after 4 days of treatment. We observed that the nanovesicles killed skin cancer cells by inducing apoptotic cell death compared to the control as revealed by TUNEL staining of xenograft tumor sections.

关键词： Squamous Cell Carcinoma Melanoma SapC-DOPS Nanovesicles Cytotoxic Effect Cancer Selectivity

Review of general, organic, and biological chemistry, second edition

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Natural Science 2014年第1期6卷 14-16页

作者： shadi abu-baker Shahrokh Ghaffari Mohannad Al-Saghir Parinbam K. Thamburaj College of Arts and Sciences Ohio University Zanesville USA

Overall, General, Organic, and Biological Chemistry is a very useful text to support a twosemester undergraduate course series in chemistry for health sciences students. Its strengths are the use of the P.A.V.E. way to student learning applications. It is very obvious that the author has the background and the preparation of the student taking the course(s) in mind when she was writing the textbook.

关键词： Text Book Review Education Chemistry Biochemistry Organic

Microwave Assisted Peptide Synthesis as a New Gold Standard in Solid Phase Peptide Synthesis: Phospholamban as an Example

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Open Journal of Synthesis Theory and Applications 2014年第1期3卷 1-4页

作者： shadi abu-baker Philip Garber Bryce Hina Trevor Reed Ghaffari Shahrokh Mohannad Al-Saghir Gary Lorigan College of Arts and Sciences Ohio University Zanesville USA College of Arts and Sciences Ohio University Zanesville USA Genesis Hospital Ohio University Zanesville USA Department of Chemistry and Biochemistry Miami University Oxford USA

In this study, we report the microwave assisted synthesis of Phospholamban protein (WT-PLB) as a new gold standard in solid phase peptide synthesis. Microwave energy offers benefits for both the coupling and deprotection reactions during peptide synthesis. The use of microwave energy for both the coupling and deprotection steps makes the microwave peptide synthesizers the most versatile and powerful systems available. It produces high yield and fast synthesis when compared to conventional peptide synthesizers.

关键词： Solid-State Peptide Synthesis Phospholamban Microwave Assisted Synthesis

Enhancing the Decomposition of Paper Cups Using Galleria Mellonella and Eisenia Fetida

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Journal of Renewable Materials 2024年第2期12卷 349-367页

作者： shadi Moqbel Habib Al-Ghoul Abd Al-Majeed Al-Ghzawi Rami Mukbel Civil Engineering Department The University of JordanAmman11942Jordan Department of Chemistry Technical University of MunichMunichGermany Plant Production Jordan University of Science and TechnologyIrbid22110Jordan Basic Medical Veterinary Sciences Jordan University of Science and TechnologyIrbid22110Jordan

The composition of paper cups creates a challenge for the recycling industry,as the paperboard–plastic film composite is hard to ***,paper cups are sent to landfills or waste *** study explores the combined use of red worms(Eisenia fetida)and Greater wax moth(Galleria mellonella)in the biodegradation of paper *** study investigates the conditions and combinations that promote using Eisenia fetida and Galleria mellonella for degrading paper *** study considered the influence of environmental temperature,the presence of food waste,varying the number of Eisenia fetida worms,and the presence of a Galleria mellonella growth-slowing agent on the degradation *** achieve the study objectives,the study followed a quantitative *** study monitored the degradation of paper cup cuts that were placed in jars containing different combinations of Eisenia fetida worms,Galleria mellonella larvae,food waste,bedding material,and Galleria mellonella growth-slowing *** study found that the best operating temperature is *** study found that using food waste improves the performance of Eisenia fetida worms and Galleria mellonella larvae *** study found that adding a Galleria mellonella growth-slowing agent slightly enhances the degradation of the paper ***,a numerical model was obtained to simulate the paper cup degradation efficiency.

关键词： Paper cups resource recovery recycling circular economy Galleria mellonella Eisenia fetida

Determinants of Occipital Involvement in Males and Females with Pattern Hair Loss: A Retrospective Study

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Journal of Cosmetics, Dermatological Sciences and Applications 2024年第2期14卷 128-140页

作者： shadi Zari Department of Dermatology College of Medicine University of Jeddah Jeddah Saudi Arabia

Background: Pattern hair loss (PHL) is the most common form of hair loss affecting both men and women. The occipital region, located at the back of the scalp, has been considered resistant to hair loss in PHL. It is often used as a donor site for hair transplantation procedures. However, evidence suggests that the occipital region may also be affected by PHL, although to a lesser extent compared to the frontal and vertex regions. Objective: To assess the levels and determinants of occipital involvement in PHL, focusing on gender and disease severity. Such data would enhance the comprehensive understanding of PHL progression and inform development of improved treatment strategies. Methods: This retrospective study involved 1993 adults (1000 males, 993 females) following for PHL at a specialized hair clinic in Jeddah, Saudi Arabia. Any patient who had hair transplantation in the past and any male who had balding areas (Hamilton-Norwood scales 4 to 7) at the initial consultation was excluded. The primary outcome was occipital involvement, defined as >10% thin hairs in the trichometry assessments of the occipital region. Additional data included patients age, treatment status, disease severity, and other trichometry parameters. Results: The prevalence of occipital involvement was found to be 23.8% overall, significantly more frequent in females (35.6%) than males with early PHL (Hamilton-Norwood scales 2 and 3) (12.1%) (p Conclusion: This study underscored the relevance of gender and disease severity in occipital involvement in PHL. Females had significantly higher rates of occipital involvement, suggesting that gender differences are critical in understanding PHL progression. Future research should aim to validate these findings, with an aim to refine therapeutic and surgical approaches to improve patient outcomes.

关键词： Pattern Hair Loss Occipital Involvement Gender Trichometry Donor Zone