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The spliceophilin CYP18-2 is mainly involved in the splicing of retained introns under heat stress in Arabidopsis

journal of Integrative Plant Biology 2023年第5期65卷 1113-1133页

作者： Areum Lee Hyun Ji Park seung hee jo Haemyeong Jung Hyun-Soon Kim Hyo-Jun Lee Youn-Sung Kim Choonkyun Jung Hye Sun Cho Plant Systems Engineering Research Center Korea Research Institute of Bioscience and Biotechnology(KRIBB)Daejeon 34141Korea Department of Biosystems and Bioengineering KRIBB School of BiotechnologyUniversity of Science and Technology(UST)Daejeon 34141Korea Department of Functional Genomics KRIBB School of BioscienceUSTDaejeon 34113Korea Department of Biotechnology NongWoo BioAnseong 17558Korea Department of International Agricultural Technology and Crop Biotechnology Institute/Green Bio Science and Technology Seoul National UniversityPyeongchang 25354Korea Department of Agriculture Forestryand Bioresources and Integrated Major in Global Smart FarmCollege of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Korea

Peptidyl-prolyl isomerase-like 1(PPIL1)is associated with the human spliceosome ***,its function in pre-mRNA splicing remains *** this study,we show that Arabidopsis thaliana CYCLOPHILIN 18-2(AtCYP18-2),a PPIL1 homolog,plays an essential role in heat tolerance by regulating pre-mRNA *** heat stress conditions,AtCYP18-2 expression was upregulated in mature plants and GFP-tagged AtCYP18-2 redistributed to nuclear and cytoplasmic *** determined that AtCYP18-2 interacts with several spliceosome complex B^(ACT)components in nuclear puncta and is primarily associated with the small nuclear RNAs U5 and U6 in response to heat *** AtCYP18-2 loss-of-function allele cyp18-2 engineered by CRISPR/Cas9-mediated gene editing exhibited a hypersensitive phenotype to heat stress relative to the wild ***,global transcriptome profiling showed that the cyp18-2 mutation affects alternative splicing of heat stress–responsive genes under heat stress conditions,particularly intron retention(IR).The abundance of most intron-containing transcripts of a subset of genes essential for thermotolerance decreased in cyp18-2 compared to the wild ***,the intron-containing transcripts of two heat stress-related genes,HEAT SHOCK PROTEIN 101(HSP101)and HEAT SHOCK FACTOR A2(HSFA2),produced functional ***101-IR-GFP localization was responsive to heat stress,and HSFA2-Ⅲ-IR interacted with HSF1 and HSP90.1 in plant *** findings reveal that CYP18-2 functions as a splicing factor within the B~(ACT)spliceosome complex and is crucial for ensuring the production of adequate levels of alternatively spliced transcripts to enhance thermotolerance.

关键词： alternative splicing CYP18-2 HSFA2-Ⅲ intron retention spliceophilin spliceosome complex B~(ACT) thermotolerance

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Maximizing biomass utilization: An integrated strategy for coproducing multiple chemicals

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journal of Energy Chemistry 2025年第1期100卷 180-191页

作者： Byeongchan Ahn Sua jo jonggeol Na J.Jay Liu Young-Ju Kim Wangyun Won Department of Chemical and Biological Engineering Korea University Department of Chemical Engineering (Integrated process) Kyung Hee University Department of Chemical Engineering and Materials Science Ewha Womans University Department of Chemical Engineering Pukyong National University Resources Engineering Plant Research Department Korea Institute of Geoscience and Mineral Resources

Lignocellulosic biomass is one of the viable solutions to alleviate the global warming. However, the limited utilization of biomass majorly focused on cellulose and hemicellulose restricts the economic and environmental feasibilities. To cope with this issue, we proposed an integrated process of co-producing 1,6-hexanediol（1,6-HDO） with tetrahydrofuran and adipic acid from biomass, referred to as Strategy A. To compare the impacts of lignin upgrading and feedstock, Strategy B, which co-produces tetrahydrofuran alone, and Strategy C, which is the traditional route to produce 1,6-HDO from fossil fuels, were used. Heat networks are also designed to reduce operating costs and indirect carbon emissions due to energy consumption, saving 87% and 83% of the heat and cooling requirements, respectively, in Strategy A. The market competitiveness of Strategy A was evaluated by determining the minimum selling price through techno-economic analysis, and sustainability was thoroughly investigated by quantifying the environmental impacts through both midpoint and endpoint life-cycle assessments（LCAs）.Strategy A was found to be the most favorable both economically（USRDSCHARDOLLAR3,402/ton） and environmentally（-26.9 kg CO2eq.）. This indicates that lignin valorization is not only economically but also environmentally preferred. Finally, changes in economic and environmental feasibilities depending on economic, process, and environmental parameters were investigated using sensitivity and uncertainty analyses. The results of these analyses provide valuable insight into bio-based chemical production.

关键词： Biorefinery Carbon mitigation Climate change Global warming Lignin utilization Bioeconomy

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Review on Tidal Energy Technologies and Research Subjects

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China Ocean Engineering 2020年第1期34卷 137-150页

作者： Chul hee jo Su Jin HWANG Department of Naval Architecture and Ocean Engineering Inha University

Tidal current power is one of the promising and reliable renewable energies with the advantage of continuous and predictable resource. It can make stable electricity regardless of weather conditions or seasons all year around. The required technologies for tidal current power in the ocean have been developed for years and now recognized that it could be commercialized after intensive field tests and successful demonstrations. There are several tidal farm development projects in the world, such as the MeyGen project in UK with its commercialization at hand. However, various research subjects in the tidal current energy field are seeking improvements and industrialization of tidal current power in terms of economy and technical reliability. This paper introduces the resource assessment procedure of tidal energy that has been applied in Korea coastal regions. The key research subjects for tidal current power together with the interaction effect of multi-arrangement is described. Also, this paper is to introduce the research output of each subject such as turbine design, experimental validation, turbine interaction and wake, multi-array module, FSI (fluid-structure interaction), and duct application.

关键词： tidal current power resource assessment turbine design experimental validation turbine interaction multi arrangement FSI(fluid-structure interaction)

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Tetradentate Pt(Ⅱ)complex as a singlet exciton sensitizing host for highly efficient green fluorescent organic light-emitting diodes

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Science China Materials 2024年第8期67卷 2618-2627页

作者： Unhyeok jo seung Chan Kim Chan hee Ryu Kang Mun Lee Jae-Min Kim Jun Yeob Lee School of Chemical Engineering Sungkyunkwan University2066Seobu-roJangan-guSuwon-siGyeonggi-do 16419Republic of Korea Department of Chemistry Institute for Molecular Science and Fusion TechnologyKangwon National UniversityChuncheonGangwon 24341Republic of Korea Department of Advanced Materials Engineering Chung-Ang University4726Seodong-daeroDaedeok-myeonAnseong-siGyeonggi-do 17546Republic of Korea SKKU Advanced Institute of Nano Technology Sungkyunkwan University2066Seobu-roJangan-guSuwonGyeonggi 16419Republic of Korea SKKU Institute of Energy Science and Technology Sungkyunkwan University2066Seobu-roJangan-guSuwonGyeonggi 16419Republic of Korea

Efficient green fluorescent organic light-emitting diodes were designed with a phosphorescent metal complex as a singlet exciton sensitizing host(SESH)for achieving high efficiency and low efficiency roll-off through exciton and polaron *** Pt(Ⅱ)complex(Pt-Trz)possessing a tetradentate ligand with a bulky blocking group not only suppresses the severe formation of excimer or dimer itself but also transfers radiative excitons to fluorescent dopant *** SESH assisted fluorescence(SESHF)device with Pt-Trz as the host exhibits the maximum external quantum efficiency of 13.3%and a lower efficiency roll-off compared with exciplex host device which is a general approach for harvesting triplet *** exciton dynamics modeling unravels that the SESHF device effectively utilizes triplet excitons in the host by intersystem crossing and resonance energy transfer from the triplet state of the host to the singlet state of the terminal *** quenching rates of the SESHF device are reduced by 11 times for triplet-triplet annihilation and 1.8 times for tripletpolaron annihilation compared with the exciplex device,which demonstrates that Pt-Trz could be a platform to be used as an efficient host for fluorophores.

关键词： singlet annihilation polaron

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Photocatalytic decomposition of mobile-source related pollutants using a continuous-flow reactor

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journal of Environmental Sciences 2010年第3期22卷 460-466页

作者： Wan-Kuen jo seung-Ho Shin Department of Environmental Engineering Kyungpook National University

This study evaluated the application of a continuous-flow photocatalytic reactor for the control of two mobile-derived pollutants, methyl-tertiary butyl ether （MTBE） and naphthalene, present at in-vehicle levels. Variables tested for this study included the hydraulic diameter （HD）, stream flow rate （SFR）, relative humidity （RH）, and feeding type （FT）. The fixed parameters included contaminant concentration, ultraviolet light source, and the weight of TiO2. In all experimental conditions the adsorption process reached equilibrium within 30 to 180 min for the target compounds, and the outlet concentrations of the photocatalytic oxidation （PCO） reactor while operating reached a steady state within 60 to 180 min. The degradation of the target compounds was dependent on RH, HD, FT, or SFR. The PCO system exhibited high degradation （up to nearly 100% for certain conditions） and mineralization efficiencies of target compounds, suggesting that this system can effiectively be employed to improve indoor air quality. Moreover, it was confirmed that trichloroethylene at urban-ambient level also could enhance the degradation efficiency of naphthalene when applying the PCO technology inside vehicles.

关键词： in-vehicle level methyl-tertiary butyl ether naphthalene trichloroethylene

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Materials and extracellular matrix rigidity highlighted in tissue damages and diseases:Implication for biomaterials design and therapeutic targets

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Bioactive Materials 2023年第2期20卷 381-403页

作者： Jae hee Park seung Bin jo Jung-Hwan Lee Hae-Hyoung Lee jonathan CKnowles Hae-Won Kim Institute of Tissue Regeneration Engineering(ITREN) Dankook UniversityCheonan31116Republic of Korea Department of Nanobiomedical Science and BK21 NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonan31116Republic of Korea Department of Biomaterials Science College of DentistryDankook UniversityCheonan31116Republic of Korea Cell&Matter Institute Dankook UniversityCheonan31116Republic of Korea Mechanobiology Dental Medicine Research Center Dankook UniversityCheonan31116Republic of Korea UCL Eastman-Korea Dental Medicine Innovation Centre Dankook UniversityCheonan31116Republic of Korea Division of Biomaterials and Tissue Engineering UCL Eastman Dental InstituteUniversity College LondonRoyal Free HospitalRowland Hill StreetLondonNW32PFUnited Kingdom

Rigidity(or stiffness)of materials and extracellular matrix has proven to be one of the most significant extracellular physicochemical cues that can control diverse cell behaviors,such as contractility,motility,and spreading,and the resultant pathophysiological *** 2D materials engineered with tunable rigidity have enabled researchers to elucidate the roles of matrix biophysical cues in diverse cellular events,including migration,lineage specification,and mechanical ***,the recent findings accumulated under 3D environments with viscoelastic and remodeling properties pointed to the importance of dynamically changing rigidity in cell fate control,tissue repair,and disease ***,here we aim to highlight the works related with material/matrix-rigidity-mediated cell and tissue behaviors,with a brief outlook into the studies on the effects of material/matrix rigidity on cell behaviors in 2D systems,further discussion of the events and considerations in tissue-mimicking 3D conditions,and then examination of the in vivo findings that concern material/matrix *** current discussion will help understand the material/matrix-rigidity-mediated biological phenomena and further leverage the concepts to find therapeutic targets and to design implantable materials for the treatment of damaged and diseased tissues.

关键词： Matrix rigidity Biophysical cue Cell and tissue engineering 3D biomaterials conditions Therapeutic targets

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joint member angle limits of composite beams and CFT square columns

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journal of Central South University 2013年第1期20卷 199-206页

作者： LEE seung-jo LEE Kang-Guk Department of Architecture Chosun College of Science & Technology Research Center for Urban Affair Kyungil University

This work aims to understand the relationship between the member angle limit and the energy ratio for 24 test samples of composite beams and CFT square steel tube columns. This work also compares the formula applicability for member angle limit with the previous test result to provide the basic data for the design of composite beam-CFT column. The evaluation of the member angle limit was performed with concrete compressive strength (fC =22.16 MPa, 30.49 MPa), breadth-to-thickness ratio (B/t=25.0, 33.3, 43.5), and axial capacity ratio (N/N0=0, 0.3, 0.4, 0.5) as the main variables of the test samples. For the relationship between the member angle limit (Ru) and the energy ratio (ES/EC ), the test result shows that the energy ratio becomes lower as the axial capacity ratio and the breadth-to-thickness ratio increase. The energy ratio is lower for the Type B test sample compared with that of Types A and C. For the formula suggested by SATO, the test samples are distributed evenly for comparison between test values and the member angle limit; however, other formulas indicate a deviation. Specifically, for the comparison between R u,cal and R u (Test), Maeda's formula shows severe deviation.

关键词： square steel tube axial capacity ratio member angle limit breadth-to-thickness ratio

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A study on the interference effects for tidal current power rotors

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Science China(Technological Sciences) 2010年第11期53卷 3094-3101页

作者： Chul-hee jo Kang-hee LEE Jin-Young YIM Department of Naval Architecture & Ocean Engineering Inha University

Due to global warming, the need to secure an alternative resource has become more important nationally. With the high tidal range of up to 9.7 m on the west coast of Korea, numerous tidal current projects are being planned and constructed. To extract a significant quantity of power, a tidal current farm with a multi-arrangement is necessary in the ocean. The rotor, which initially converts the energy, is a very important component because it affects the efficiency of the entire system, and its performance is determined by various design variables. The power generation is strongly dependent on the size of the rotor and the incoming flow velocity. However, the interactions between devices also contribute significantly to the total power capacity. Therefore, rotor performance considering the interaction problems needs to be investigated for generating maximum power in a specific field. This paper documents a performance study of devices considering the interference between rotating rotors with axial, transverse and diagonal arrangements.

关键词： TCP (tidal current power) renewable energy interference effect rotor CWC (circulation water channel) CFD (computational fluid dynamics)

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A new damage quantification approach for shear-wall buildings using ambient vibration data

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Frontiers of Structural and Civil Engineering 2015年第1期9卷 17-25页

作者： seung-Hun SUNG Hyung-jo JUNG Department of Civil and Environmental Engineering Korea Advanced Institute of Science and Technology Daejeon 305- 701 Korea

This paper presents a new approach to estimate damage seventy tor slaear-wall DUlltllngs using alagona~ terms of a modal flexibility matrix estimated from dynamic properties. This study aims to provide a fundamental concept for quantifying the damage of realistic buildings by investigating an idealized shear-wall building. Numerical studies were performed on a 5-story shear-wall building model to validate the applicability of the presented approach, using two damage patterns. With the numerical simulations, the proposed approach accurately determined the damage ratio of the specimens. Experiments were also conducted on a 5-story shear-wall building model for which the system parameters were almost the same as those in numerical simulations. The estimated damage-quantification results from the experimental validations demonstrated that the performance of the presented method for shear-wall buildings was both suitable and accurate.

关键词： damage identification modal flexibility damage quantification shear-wall buildings

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Segregation of voiced and unvoiced components from residual of speech signal

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journal of Central South University 2012年第2期19卷 496-503页

作者： jo Cheol-woo KIM Jae-hee School of Mechatronics Changwon National University

In conventional source-filter models, voiced and unvoiced components were considered independently. However, in practice it was difficult to separate the source into two parts. An actual source consists of a mixture of two sources and the ratio varies according to the content or the intention of speaker. It had been investigated to separate the voiced and unvoiced components for different source models. Source signals were modeled based on the residual signal measured from inverse filtering. Three different source models were assumed. The parameters of each model were optimized for the original speech signal using a genetic algorithm. The resulting parameters were compared in terms of the mel-cepstral distance to the original signal, the spectrogram and the spectral envelope from the synthesized signal. The optimization method achieves an improvement of 15% for the Klatt model, but there is little improvement in the modified residual case.

关键词： voice source model synthesis optimization genetic algorithm

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