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Development, Validation and Application of a Spectrofluorimetric Method for the Quantification of Nevirapine in Pharmaceutical Formulations Tablets and Suspensions

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American Journal of Analytical Chemistry 2022年第6期13卷 206-227页

作者： Lucien Murhula Namegabe Aladin Ombeni Mahano serigne omar sarr Official University of Bukavu Faculty of Pharmaceutical Sciences and Public Health Bukavu Bukavu Democratic Republic of the Congo Cheikh Anta Diop University Analytical Chemistry and Food Sciences Laboratory Dakar Senegal National Medecines Control Laboratory Dakar Senegal

The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirapine by spectro-fluorimetric has been reported. The proposed method is based on the transformation of naturally non-fluorescent nevirapine into a fluorescent derivative after chemical synthesis. Maximum excitation and emission wavelengths are 290 nm and 357 nm respectively. The analytical performance of the method demonstrates linearity in the concentration range 1.5 × 10-2 and 13.5 × 10-2 μg/mL with a correlation coefficient (r) greater than 0.999. The detection (LOD) and quantification (LOQ) limits found are 1.97 × 10-3 μg/mL and 5.48 × 10-3 μg/mL respectively. Recovery is achieved with 99.9% and 100.3% trueness, intra-day precision with a coefficient of variation of repeatability (CVr) of 0.99% and inter-day precision with a coefficient of variation of precision (CVR) of 1.7%. The method has been successfully applied in the analysis of 10 batches of nevirapine tablets and suspensions.

关键词： Spectrofluorimetric Method Validation Nevirapine Sodium Hypochlorite

Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules

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American Journal of Analytical Chemistry 2018年第3期9卷 162-170页

作者： Lucien Murhula Namegabe serigne omar sarr Yérim Mbagnick Diop Department of Galenic Pharmacy Official University of Bukavu Bukavu Democratic Republic of the Congo Analytical Chemistry and Food Sciences Laboratory Cheikh Anta Diop University Dakar Senegal National Medecines Control Laboratory Dakar Senegal

The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was observed between 0.25 μg/mL and 1.5 μg/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 μg/mL and 0.0412 μg/mL respectively. The proposed method proved trueness with a recovery between 99.88% and 101.10%. The relative standard deviations of repeatability and intermediate precision found ≤2.88% reflected a good precision of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards.

关键词： Validation Spectrofluorimetry Tetracycline

Validation of a Capillary Electrophoresis Method for Analyzing Chlorphenamine Maleate-Based Drugs Using the Accuracy Profile Approach

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American Journal of Analytical Chemistry 2016年第5期7卷 452-459页

作者： serigne omar sarr Eddy Kevin Senan Ouendo Thierno Mouhamed Wane El Hadji Assane Diop Diop Amadou serigne Momar Ndiaye Rokhaya Gueye Khadidiatou Thiam Aminata sarr Ousmane Niass Djibril Fall Bara Ndiaye Serge Rudaz Yérim Mbagnick Diop Laboratory of Analytical Chemistry and Food Sciences Cheikh Anta Diop University Dakar Senegal National Medicines Control Laboratory Ministry of Health Dakar Senegal Laboratory of Pharmaceutical Analytical Chemistry School of Pharmacy Geneva Switzerland Laboratory of Organic and Therapeutic Chemistry Cheikh Anta Diop University Dakar Senegal

This study aimed at validating an analytical method, using the accuracy profile approach, for the assay of chlorphenamine maleate by capillary electrophoresis. The validation was done using concentrations ranging between 75% and 125% of the target concentration of 600 mg/ml. Validation standards were prepared separately in triplicate for each series. Studied validation criteria were selectivity, linearity, trueness, precision (repeatability and intermediate precision), accuracy and limits of detection and quantification. The method was selective, with recoveries ranging between 99.55% and 99.84%. The relative standard deviations of repeatability and intermediate precision were <5%. The accuracy profile confirmed the performance of the assay method between 75% and 100% of the target concentration of 600 mg/ml. The detection and quantification limits were 5 mg/l and 15 mg/l respectively. This ecological and economical method was applied to identify and quantify chlorphenamine maleate in 3 samples of chlorphenamine maleate-based drugs provided by the Senegalese National Medicines Control Laboratory. All analyzed samples were in accordance with official standards.

关键词： Analytical Validation Chlorphenamine Maleate Quality Capillary Electrophoresis CEB Senegal

Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods

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Food and Nutrition Sciences 2022年第11期13卷 950-961页

作者： Rokhaya Gueye Viviane Chatchueng Sandefo Babacar Beye Elhadji Ousmane Faye Amadou Diop serigne omar sarr Bara Ndiaye Yérim Mbagnick Diop Laboratoire de Chimie Analytique et Bromatologie Faculté de Médecine de Pharmacie et d’Odontologie-Stomatologie Université Cheikh Anta DIOP Dakar Sénégal Laboratoire de Mycotoxines Institut de Technologie Alimentaire Route des Pères Maristes Dakar Sénégal

Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.

关键词： Poultry Feed Aflatoxin High Performance Liquid Chromatography Thin-Layer Chromatography Dakar

Iodine 131 Treatment in Graves’ Disease in a West African Country: Preliminary Study about 25 Cases in Senegal

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Open Journal of Biophysics 2024年第1期14卷 56-72页

作者： El Hadji Amadou Lamine Bathily serigne Moussa Badiane Mamoudou Salif Djigo Gora Thiaw Kalidou Gueye Ousseynou Diop Boucar Ndong Kokou Fofo Toussaint Adambounou Alphonse Rodrigue Djiboune Papa Mady Sy Mamadou Soumbounou Mohamed Chekhma Louis Augustin Diaga Diouf Gora Mbaye omar Ndoye Mounibé Diarra Mamadou Mbodj Nuclear Medicine Department General Hospital of Idrissa Pouye (HOGIP) Dakar Senegal Biophysical Laboratory Cheikh Anta Diop University (UCAD) Dakar Senegal Biophysical Laboratory Gaston Berger University (UGB) Saint-Louis Nuclear Medicine Department Hospital Dalal Jamm Dakar Senegal Biophysical Laboratory University of Lome Lome Togo Laboratory of Physical and Pharmaceutical Biophysics Cheikh Anta Diop University Dakar Senegal

Introduction: Graves’ disease is the most common cause of hyperthyroidism. Its treatment uses synthetic antithyroid drugs but the use of aggressive radical therapy such as surgery or non-aggressive therapy such as iodine-131 is not uncommon. Treatment of Graves’ disease with radioactive iodine or iratherapy is a simple, inexpensive, well-tolerated treatment. It was introduced in Senegal in 2016. We report through this work the preliminary assessment of the only nuclear medicine service in Senegal in the management of Graves’ disease by iodine-131. Patients and Methods: Retrospective study of the first cases of Graves’ disease treated with iratherapy in Senegal. Socio-demographic, clinical, paraclinical, therapeutic and evolutionary aspects were studied. Radiation protection rules have been implemented and contraception has been effective for six months in women of childbearing age. Results: 25 patients were collected with a mean age of 45 years, twenty women (80%), a family goiter in 24% and a psycho-affective context in 64% of cases. Thyrotoxicosis syndrome was associated with goiter in 68% of patients and exophthalmos in 64%. Thyroid ultrasound performed in 20 patients showed vascular goiter in 80% and thyroid scintigraphy in 3 patients, homogeneous and diffuse hyperfixation. TRAK dosed in 8 patients was still positive. All patients had received first-line medical treatment. The average duration of this treatment was more than 18 months in 92%. The empirically used iodine-131 activity averaged 15.35 mCi. Oral corticosteroid therapy was prescribed in 7 patients for the prevention of malignant orbitopathy. No early side effects were noted. The remission rate at 3 months was 52% and at 6 months was 88% to 92%. Conclusion: The effectiveness of radioactive iodine, in particular ablative doses in the treatment of hyperthyroidism, is no longer to be demonstrated. Taking into account our socioeconomic context, iratherapy should be a treatment of choice for hyperthyroidism

关键词： Graves’ Disease Iratherapy Iodine-131 Senegal

Contribution of Bone Scintigraphy in the Diagnosis of a Case of SAPHO in the Nuclear Medicine Department of Idrissa Pouye General Hospital (Dakar, Senegal)

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Open Journal of Medical Imaging 2024年第1期14卷 1-9页

作者： El Hadji Amadou Lamine Bathily Kalidou Gueye serigne Moussa Badiane Mamoudou Salif Djigo Gora Thiaw Ousseynou Diop Olatounde Herbert Fachinan Papa Mady Sy Alfonse Rodrigue Djiboune Kokou Fofo Toussaint Adambounou Louis Augustin Diaga Diouf Boucar Ndong Gora Mbaye omar Ndoye Mounibé Diarra Mamadou Mbodj Nuclear Medicine Department Idrissa Pouye General Hospital (HOGIP) Dakar Senegal Biophysical Laboratory FMPO Cheikh Anta Diop University (UCAD) Dakar Senegal Biophysical Laboratory UFR2S Gaston Berger University (UGB) Saint-Louis Senegal Nuclear Medicine Department Hospital Dalal Jamm Dakar Senegal Laboratory of Physical and Pharmaceutical Biophysics FMPO Cheikh Anta Diop University Dakar Senegal Biophysical Laboratory University of Lome Lome Togo

Introduction: The acronym SAPHO (Synovitis, Acne, Pustulosis, Hyperostosis and Osteitis) is a syndrome combining osteoarticular and cutaneous manifestations. It occurs mainly between the ages of 30 and 50. Sternocostoclavicular hyperostosis is one of the main distinguishing features. We report a case of SAPHO in Dakar diagnosed by bone scintigraphy. Observation: 28-year-old Senegalese women presented with left shoulder pain and relative functional impotence for over 2 years. Examination revealed right sternoclavicular hyperostosis and left shoulder pain on palpation. Questioning revealed a history of acne and hyperostosis of the right first toe. Bone scintigraphy, performed after injection of 630 MBq of 99mTc-HMDP, revealed: hyperfixation of the bilateral (right++) manubrio-sternal and sternoclavicular junction, producing the classic bull’s horn image;hyperfixation of the left shoulder with an inflammatory appearance;hyperfixation of the sacroiliac joints suggestive of bilateral sacroiliitis;hyperfixation of the right first toe;two mandibular hyper fixations probably related to dental damage. This scintigraphic appearance in one was strongly suggestive of SAPHO syndrome. Conclusion: SAPHO syndrome, related to spondyloarthropathy, associates cutaneous and osteoarticular signs. It is characterized by frequent delays in diagnosis due to poor recognition. Soy is an invaluable diagnostic tool, enabling us to assess the extent of the disease and its evolution.

关键词： SAPHO Bone Scintigraphy Young Woman Senegalese

Audit of the Physico-Chemical Quality of Amoxicillin Based Drugs Marketed in Senegal

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American Journal of Analytical Chemistry 2023年第9期14卷 351-361页

作者： Coulibaly Dalané Bernadette Sow Fatoumata Tata Naco Mohamed El Béchir N’Diaye Mahamar Toure Hamadoun Abba Mariko Madani Dackouo Blaise Diouf Diéry Koumare Benoit Yaranga sarr serigne omar Faculty of Medicine Pharmacy and Odonto-Stomatology University Cheik Anta DIOP Dakar Dakar Sénégal Faculty of Pharmacy of Bamako University of Technical and Technological Sciences of Bamako (FAPH/USTTB) Bamako Mali National Health Laboratory of Mali Bamako Mali National Drug Control Laboratory of Senegal (LNCM) Dakar Sénégal

Non-compliant antibiotics pose problems of therapeutic efficacy at the level of the individual, but also a serious public health problem. The objective of our study was to evaluate the quality of generic amoxicillin (42 samples) by analytical methods in Dakar/Senegal. At 100% visual inspection of compliance;100% compliance uniformity test;at the dissolution test all > 80% (86.8% to 108.8%). The presence of amoxicillin with the following contents: Tablets (95.2% to 104.1%);Capsules (91.5% to 113.9%) and Suspensions drinkable (96.9% - 118.7%). All the samples in our study are 100% compliant according to the European and American pharmacopoeias (90% - 120%). Other studies should therefore be oriented in the direction of stability as well as the dosage of degradation products.

关键词： Quality Control Amoxicillin Analytical Methods

Natural Enemies and Pest Control in Field-Grown Crop in Southern Senegal

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Advances in Entomology 2022年第4期10卷 287-299页

作者： Etienne Tendeng Babacar Labou El Hadji serigne Sylla Amadou Baldé Mamadou Diatte Oumar Seydi Issa Alé Ndiaye Pape Diop serigne omar Sène Saliou Djiba Karamoko Diarra UCAD Integrated Production and Protection Laboratory in Agroecosystems (L2PIA) Faculty of Science and Technology Cheikh Anta Diop University Dakar Senegal ISRA Entomology Laboratory of Djibélor Agricultural Research Centre Senegalese Institute for Agricultural Research Ziguinchor Senegal

Crop pests are a permanent threat to horticultural production. Several control methods are recommended for their control, including biological control. This method based on the use of natural enemies is not well established. The objective of this study was to assess the diversity and abundance of natural enemies of crop pests. A survey of crops associated with pests was carried out on 144 fields in southern Senegal to measure the diversity and abundance of natural enemies of various crop pests, determine the native parasitoid complex and assess parasitism rates. Ecological indices were calculated to assess the diversity of natural enemies. A total of 25 natural enemy species were identified, including 15 parasitoids and 10 predators. Predatory Hemiptera and Parasitoid Hymenoptera were high biodiversity. The parasitism rates vary between 0 and 50% depending on the abundance of pests and parasitoid species. Knowledge of the diversity of natural enemies and the understanding of trophic interactions with pests are important in the development of biological crop protection in order to preserve resilience in agroecosystems.

关键词： Biodiversity Agroecosystem Crop Protection Pests Market Gardening Biological Control

First Record of Pristomerus pallidus (Hymenoptera: Ichneumonidae: Cremastinae) (Kriechbaumer) Parasitizing Hellula undalis (F.) (Lepidoptera: Pyralidae) in Crucifer Crop in Senegal

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Advances in Entomology 2023年第4期11卷 256-263页

作者： Etienne Tendeng Etienne Tendeng Babacar Labou El Hadji serigne Sylla Amadou Baldé Mamadou Diatte Oumar Seydi Issa Alé Ndiaye Pape Diop serigne omar Sène Saliou Djiba Karamoko Diarra Integrated Production and Protection Laboratory in Agroecosystems (L2PIA) Faculty of Science and Technology Cheikh Anta Diop University (UCAD) Dakar Senegal Entomology Laboratory of Djibé lor Agricultural Research Centre Senegalese Institute for Agricultural Research (ISRA) Ziguinchor Senegal

Cabbage is today an economically important crop grown in more than 90 countries around the world. Production is constantly threatened by pests including the cabbage webworm, Hellula undalis (F.) (Lepidoptera: Pyralidae) which just one or two larvae can cause severe damage by infesting the growing points or shoots of young plants. Hellula undalis is difficult to control by the use of chemical insecticides. The establishment of effective biological control is needed. The parasitoid species Pristomerus pallidus (Hymenoptera: Ichneumonidae: Cremastinae) was recorded for the first time in Senegal on larvae of Hellula undalis from cabbage fields infested with H. undalis. Laboratory observed parasitism was 7.4%. This is the first record of Pristomerus pallidus parasitizing Hellula undalis from Senegal. Furthermore, studies on the potential regulation of Hellula undalis under controlled and field conditions need to be evaluated for utilizing this parasitoid as a biocontrol agent against the cabbage webworm. .

关键词： Biological Control Parasitoid Pest Market Gardening Ziguinchor Casamance