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Microsurgical tunica albuginea transplantation in an animal model

Asian Journal of Andrology 2017年第6期19卷 694-699页

作者： Salvatore Sansalone Carla Loreto Rosario Leonardi Giuseppe Vespasiani Giuseppe Musumeci Claudia Lombardo sergio castorina Venera Cardile Rosario Caltabiano Department of Experimental Medicine and Surgery Tor Vergata University Rome Italy Department of Biomedical and Technological Sciences Section of Human Anatomy and Histology University of Catania Catania Italy Musumeci CLinic GECS Catania Italy Department of Bio-Medical Sciences Section of Physiology University of Catania Catania Italy Department of Medical and Surgical Sciences and Advanced Technology "G.E Ingrassia" Section of Anatomic Pathology University of Catania Catania Italy.

Several andrological diseases require surgical repair or reconstruction of tunica albuginea, which envelops the corpora cavernosa penis. Despite intense research efforts involving a variety of biological materials, such as skin, muscle aponeurosis, human dura mater, tunica vaginalis, and pericardium, engineered tunica albuginea suitable for graft use is yet to be obtained. The study investigates microsurgical tunica albuginea allotransplantation in an animal model with the purpose of creation of an organ-specific tissue bank to store penile tissue, from cadaveric donors and male-to-female trans-sexual surgery, for allogeneic transplantation. Materials were tunica albuginea tissue explanted from 15 donor rats, cryopreserved at -80~C, gamma-irradiated, and implanted in 15 recipient rats, of which three rats were used as controls. Penile grafts were explanted at different time intervals; after macroscopic evaluation of the organ, the grafts were processed to morphological, histochemical, and immunohistochemical examinations by light microscopy. Detection of pro-inflammatory cytokines was also performed. Examination of the tunica albuginea allografts collected 1, 3, or 6 months after surgery and of control tunica albuginea fragments showed that tunica albuginea implants achieved biointegration with adjacent tissue at all-time points. The integration of cryopreserved rat tunica albuginea allografts, documented by our study, encourages the exploration of tunica albuginea allotransplantation in humans. In conclusion, the effectiveness and reliability of the tunica albuginea conditioning protocol described here suggest the feasibility of setting up a tunica albuginea bank as a further tissue bank.

关键词： penile tunica albuginea rat model transplant

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TRAIL Immunolocalisation in the Rat Periodontal Ligament during Experimental Tooth Movement. A Preliminary Study

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Open Journal of Apoptosis 2013年第3期2卷 31-36页

作者： Carla Loreto Giuseppe Musumeci sergio castorina Jessica Valentino Salvatore Giunta Rosalia Leonardi Department of Bio-Medical Sciences Anatomy and Histology Section University of Catania Catania Italy Department of Orthodontics University of Catania Catania Italy

The aim of this study was to evaluate immunohistochemically TRAIL (TNF-Related Apoptosis Inducing Ligand) expression in the periodontal ligament of rats’ molars considering its distribution pattern in the tension and compressive regions of ligament during orthodontic movement. Sixteen Sprague-Dawley rats, weighing between 120 and 200 gwere used in the present study. Tooth movement was induced by placing elastic bands between the maxillary first and second molars. The elastic bands were left in place for 12 (time T1) and 24 (time T2) hours. After rats’ euthanasia, maxillae were dissected free and the sections were exposed to a primary rabbit polyclonal anti-TRAIL anti-body. The staining status was identified as either negative or positive by using 3,3’-diaminobenzidine (DAB) to develop the immunoreaction. Data were analyzed using ANOVA test and a post-hoc Dunn’s Multiple Comparison test. P-values of less than 0.05 were considered statistically significant. The staining for TRAIL in the tension side appeared darker (intensity of staining = 2) than in the compressive side (intensity of staining = 1) 12 h after the tooth movement. The stainings of both sides of PDL were observed to be up-regulated 24 h after the application of the force (tension side = 3, compression side = 2;P 0.05). TRAIL is expressed in the periodontal ligament of rats’ molars during experimental tooth movement both in the compression and in the tension sides. The latter showed the highest amount of TRAIL immunolabeling.

关键词： Immunohistochemistry Pperiodontal Ligament Tooth Movement Trail

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New perspectives for articular cartilage repair treatment through tissue engineering: A contemporary review

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World Journal of Orthopedics 2014年第2期5卷 80-88页

作者： Giuseppe Musumeci Paola Castrogiovanni Rosalia Leonardi Francesca Maria Trovato Marta Anna Szychlinska Angelo Di Giunta Carla Loreto sergio castorina Department of Bio-Medical Sciences Human Anatomy and Histology Section School of Medicine University of Catania 95123 Catania Italy Department of Medical and Surgical Sciences Ⅱ Dental Unity University of Catania 95123 Catania Italy Department of Medical and Pediatric Sciences Internal Medicine Division University of Catania 95123 Catania Italy Fondazione Mediterranea ‘‘G.B. Morgagni’’ 95125 Catania Italy

In this paper review we describe benefits and disadvantages of the established methods of cartilage regeneration that seem to have a better long-term *** illustrated the anatomical aspect of the knee joint cartilage, the current state of cartilage tissue engineering, through mesenchymal stem cells and biomaterials,and in conclusion we provide a short overview on the rehabilitation after articular cartilage repair *** articular cartilage has low capacity to repair itself,and thus even minor injuries may lead to progressive damage and osteoarthritic joint degeneration, result-ing in significant pain and disability. Numerous efforts have been made to develop tissue-engineered grafts or patches to repair focal chondral and osteochondral defects, and to date several researchers aim to implement clinical application of cell-based therapies for cartilage repair. A literature review was conducted on PubM ed, Scopus and Google Scholar using appropriate keywords, examining the current literature on the wellknown tissue engineering methods for the treatment of knee osteoarthritis.

关键词： Cartilage Repair Mesenchymal stem cells Scaffolds Tissue engineering Osteoarthritis

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