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The effect of long-term application of nitrogen-rich fertilizers on soil resistome:A study of conventional and organic cropping systems

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Soil Ecology Letters 2024年第3期6卷 135-150页

作者： Alexey S.vasilchenko Evgenii O.Burlakov Darya v.Poshvina Denis S.Gruzdev sergey v.Kravchenko Aleksandr v.Iashnikov Ning Ling Anastasia v.vasilchenko Laboratory of Antimicrobial Resistance Institute of Environmental and Agricultural Biology(X-BIO)Tyumen State UniversityTyumenRussia Research Institute of Mathematics Physics and Computer ScienceDerzhavin Tambov State UniversityTambovRussia International Integrated Research Laboratory for Climate Change Land Use and BiodiversityInstitute of Environmental and Agricultural Biology(X-BIO)Tyumen State UniversityTyumenRussia SciBear OU Tartu Mnt 67/1-13bKesklinna Linnaosa10115 TallinnEstonia Center for Grassland Microbiome State Key Laboratory of Grassland Agro-EcosystemsCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000China

Metagenomic studies of various soil environments have previously revealed the widespread distribution of antibiotic resistance genes(ARGs)around the *** this study,we applied shotgun metagenomics to investigate differences in microbial communities and resistomes in Chernozem soils that have been under long-term organic and conventional cropping *** organic cropping system was seeded with Triticum spelta without any *** conventional cropping system was seeded with Tríticum durum Desf and used mineral fertilizer(NPK),that resulted in an increased amount of total and available carbon and nitrogen in *** all samples,we identified a total of 21 ARG classes,among which the dominant were vancomycin,tetracycline and *** of soil microbial communities revealed differences between the studied fields in the relative abundances of 14 and 53 genera in topsoil and subsoil,*** analysis showed significant correlations(positive and negative)among 18 genera and 6 ARGs,as well as between these ARGs and some chemical properties of *** analysis of metagenome-assembled genomes revealed that Nitrospirota,Thermoproteota,Actinobacteriota and Binatota phyla of archaea and bacteria serve as hosts for glycopeptide and fluoroquinolone/tetracycline ***,the data obtained enrich knowledge about the consequences of human agricultural activities in terms of soil microbiome modification and highlight the role of nitrogen cycling taxa,including uncultivated genera,in the formation of soil resistome.

关键词： soil microbiome inorganic fertilizer nitrogen cycle uncultured bacteria chemolithotrophs Binatia

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Intelligent Robust Control of Redundant Smart Robotic Arm Pt I: Soft Computing KB Optimizer - Deep Machine Learning IT

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Artificial Intelligence Advances 2020年第1期2卷 31-58页

作者： Alena v.Nikolaeva sergey v.Ulyanov Dubna State University Universitetskaya Str.19DubnaMoscow Region141980Russia ISESYS LLC(EFKO GROUP) Naberezhnaya Ovchinnikovskaya Str.20 Bld.2MoscowRussia

Redundant robotic arm models as a control object *** of computational intelligence IT on soft computing optimizer of knowledge base in smart robotic manipulators *** computing optimizer is the sophisticated computational intelligence toolkit of deep machine learning SW platform with optimal fuzzy neural network *** methods for development and design technology of control systems based on soft computing introduced in this Part 1 allow one to implement the principle of design an optimal intelligent control systems with a maximum reliability and controllability level of a complex control object under conditions of uncertainty in the source data,and in the presence of stochastic noises of various physical and statistical *** knowledge bases formed with the application of soft computing optimizer produce robust control laws for the schedule of time dependent coefficient gains of conventional PID controllers for a wide range of external perturbations and are maximally insensitive to random variations of the structure of control *** robustness is achieved by application a vector fitness function for genetic algorithm,whose one component describes the physical principle of minimum production of generalized entropy both in the control object and the control system,and the other components describe conventional control objective functionals such as minimum control error,*** application of soft computing technologies(Part I)for the development a robust intelligent control system that solving the problem of precision positioning redundant(3DOF and 7 DOF)manipulators *** of quantum soft computing in robust intelligent control of smart manipulators in Part II described.

关键词： Intelligent control system Knowledge base Soft computing technology Decomposition Redundant robotic manipulator

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Intelligent Robust Control of Redundant Smart Robotic Arm Pt II: Quantum Computing KB Optimizer Supremacy

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Artificial Intelligence Advances 2020年第2期2卷 32-67页

作者： Alena v.Nikolaeva sergey v.Ulyanov Dubna State University Russia INESYS LLC(EFKO GROUP) Russia Physical and Mathematical Sciences JINRRussia LIT JINR INESYS LLC(EFKO GROUP)Russia

This article is a continuation of the work“Intelligent robust control of redundant smart robotic arm Pt I:Soft computing KB optimizer-deep machine learning IT”.In the first part of the paper,we examined control systems with constant coefficients of the conventional PID controller(based on genetic algorithm)and intelligent control systems based on soft computing *** demonstration,MatLab/Simulink models and a test benchmark of the robot manipulator *** and limitations of intelligent control systems based on soft computing technology *** main element of the control system based on soft computing is a fuzzy controller with a knowledge base in *** the first part of the article,two ways to implement fuzzy controllers *** way applyied one controller for all links of the manipulator and showed the best ***,such an implementation is not possible in complex control objects,such as a manipulator with seven degrees of freedom(7DOF).The second way use of separated control when an independent fuzzy controller controls each *** control decomposition due to a slight decrease in the quality of management has greatly simplified the processes of creating and placing knowledge *** this Pt II,to eliminate the mismatch of the work of separate independent fuzzy controllers,methods for organizing coordination control based on quantum computing technologies to create robust intelligent control systems for robotic manipulators with 3DOF and 7DOF *** supremacy of developed end-to-end IT design of robust intelligent control systems demonstrated.

关键词： Quantum computing supremacy Quantum-classical correlation Knowledge base Fuzzy controller Quantum fuzzy inference

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Phylogeny of Brucella abortus strains isolated in the Russian Federation

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Asian Pacific Journal of Tropical Medicine 2021年第7期14卷 323-329页

作者： Dmitry A.Kovalev Dmitriy G.Ponomarenko sergey v.Pisarenko Nikolay A.Shapakov Anna A.Khachaturova Natalia S.Serdyuk Olga v.Bobrysheva Alexander N.Kulichenko Stavropol Research Anti-Plague Institute 355035StavropolRussian Federation

Objective: To study Brucella(B.) abortus strains isolated in the Russian Federation, in order to identify their detailed position in the phylogenetic structure of the species global population as well as to determine genetic relationships for isolates from different geographical ***: Based on Bayesian method, the whole genome singlenucleotide polymorphism(SNP) analysis of 258 B. abortus strains from different geographical areas of the world including 20 B. abortus strains isolated in Russia was carried out. Results: The core genome SNP analysis of the B. abortus isolates allowed describing the main genetic lineages. The Russian strains entered two separate clades, including the basal branch and the C1 branch that is widely spread in Eurasia. The data on the isolation time was used for the dating of phylogenetic tree, and also the estimated time frame for the B. abortus genotype diversification was determined. There were sets of specific SNPs identified, which defined each of the genotypes and ***: A significant genetic diversity of the brucellosis pathogen strains from Russia has been proven. The sets of unique specific SNPs described in our study may become one of the elements within a bio-informational analysis algorithm to be used for epidemiological study of brucellosis outbreaks, including those caused by new(atypical) genetic variants of B. abortus.

关键词： Brucella abortus Phylogeny Evolution Genome SNP

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Classification,diagnosis and treatment of ANCA-associated vasculitis

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World Journal of Rheumatology 2015年第1期5卷 36-44页

作者： sergey v Moiseev Pavel I Novikov Clinic of Nephrology Internal and Occupational Diseases The Sechenov First Moscow State Medical University

Diagnosis of anti-neutrophil cytoplasmic antibodies(ANCA)-associated vasculitis is usually not difficult in patient with systemic disease, including lung and kidneys involvement, and laboratory signs of inflammation. The presence of ANCA and the results of histological investigation confirm diagnosis of ANCAassociated vasculitis. Cyclophosphamide/azathioprine in combination with high dose steroids are used to induce and maintain remission of systemic vasculitis. The clinical trials also showed efficacy of rituximab that induces depletion of B-cells. Our understanding and management of ANCA-associated vasculitis improved significantly over the last decades but there is still a lot of debate over its classification, diagnostic criteria, assessment of activity and optimum treatment.

关键词： Systemic vasculitis Anti-neutrophil cytoplasmic antibodies Granulomatosis with polyangiitis Microscopic polyangiitis

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Investigation of radio pulsar emission features using power spectra

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Research in Astronomy and Astrophysics 2018年第8期18卷 63-70页

作者： valery M.Malofeev sergey A.Tyul'bashev EN.Lebedev Physical Institute of the Russian Academy of Sciences V.V.Vitkevich Pushchino Radio Astronomy ObservatoryPRAO ASC FIANPushchinoRussia

Since 2013, round-the-clock monitoring of the sky has been carried out simultaneously in 96 beams using a high-sensitivity radio telescope called the Large Phased Array （LPA） at the frequency 110.25 MHz. These observations are made under the program of interplanetary plasma investigation. The same data are used to search for pulsars by means of power spectra. To increase the sensitivity of the pulsar search, 500-600 power spectra corresponding to different days of observations are summed. In the integrated spectra of known pulsars, besides expected improvement in signal-to-noise （S/N） ratio for the frequency harmonics, some features are explored in this paper. We present the 27 strongest pulsars which are in a field with declination 21° - 42°. The observable details in the integrated power spectra are connected with the presence of pulsar periods of the second （P2） and third （P3） class, which have been identified. Empirical relations for calculating these periods are obtained. The value P2 is estimated for 26 pulsars, and for 15 sources it is made for the first time. The value P3 is estimated for 13 pulsars, among them these values are given for five sources for the first time.

关键词： pulsars general - pulsars

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Observation of nonlinear disclination states

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Light(Science & Applications) 2023年第9期12卷 1818-1829页

作者： Boquan Ren Antonina A.Arkhipova Yiqi Zhang Yaroslav v.Kartashov Hongguang Wang Sergei A.Zhuravitskii Nikolay N.Skryabin Ivan v.Dyakonov Alexander A.Kalinkin Sergei P.Kulik victor O.Kompanets sergey v.Chekalin victor N.Zadkov Key Laboratory for Physical Electronics and Devices Ministry of EducationSchool of Electronic Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China Institute of Spectroscopy Russian Academy of SciencesTroitskMoscow 108840Russia Faculty of Physics Higher School of EconomicsMoscow 105066Russia Quantum Technology Centre Faculty of PhysicsM.V.Lomonosov Moscow State UniversityMoscow 119991Russia

Introduction of controllable deformations into periodic materials that lead to disclinations in their structure opens novel routes for construction of higher-order topological insulators hosting topological states at *** of these topological states is consistent with the bulk-disclination correspondence principle,and is due to the filling anomaly that results in fractional charges to the boundary unit *** far,topological disclination states were observed only in the linear regime,while the interplay between nonlinearity and topology in the systems with disclinations has been never studied *** report here on the experimental observation of the nonlinear photonic disclination states in waveguide arrays with pentagonal or heptagonal disclination cores inscribed in transparent optical medium using the fs-laser writing *** transition between nontopological and topological phases in such structures is controlled by the Kekulédistortion coefficient r with topological phase hosting simultaneously disclination states at the inner disclination core and spatially separated from them corner-I,corner-II,and extended edge states at the outer edge of the *** show that the robust nonlinear disclination states bifurcate from their linear counterparts and that location of their propagation constants in the gap and,hence,their spatial localization can be controlled by their *** disclination states can be efficiently excited by Gaussian input beams,but only if they are focused into the waveguides belonging to the disclination core,where such topological states *** results open new prospects for investigation of nonlinear effects in topological systems with disclinations and are relevant for different areas of science,including Bose-Einstein and polariton condensates,where potentials with the disclinations can be created.

关键词： structure topological nonlinear

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Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconversion nanoparticles

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Nano Research 2019年第12期12卷 2933-2940页

作者： Alexey B.Kostyuk Artem D.vorotnov Andrey v.Ivanov Arthur B.volovetskiy Aleksandr v.Kruglov Lyudmila M.Sencha Liuen Liang Evgenii L.Guryev vladimir A.vodeneev sergey M.Deyev Yiqing Lu Andrei v.Zvyagin Lobachevsky State University of Nizhny Novgorod Nizhny Novgorod 603950Russia Center of Biomedical Engineering Institute of Molecular MedicineSechenov UniversityMoscow 119991Russia ARC Centre of Excellence"Nanoscale BioPhotonics" Department of Physics and AstronomyMacquarie UniversitySydney 2109Australia Institute ofBioorganic Chemistry Russian Academy of SciencesMoscow 117997Russia

High-contrast optical imagi ng is achievable using phosphoresce nt labels to suppress the short-lived background due to the optical backscatterand autofluoresce ***,the long-lived phosphorescence is generally incompatible with high-speed laser-scan ning imaging ***,we show that upc on versi on nan oparticles of structure NaYF4:Yb co-doped with 8%Tm(8T-UCNP)in combi nation with a commerciallaser-scanning multiphoton microscopy are uniquely suited for labeling biological systems to acquire high-resolution images with the enhancedcon *** comparison with many phosphoresce nt labels,the 8T-UCNP emission lifetime of-15μs affords rapid image ***-order optical nonlinearity of the 8T-UCNP(n=4,as confirmed experimentally and theoretically)afforded pushing the resolution limitattain able with UCNPs to the *** contrast enha nceme nt was achieved by suppressing the backgro und using(i)ban dpassspectral filtering of the narrow emission peak of 8T-UCNP at 455-nm,and(ii)time-gating implemented with a time-correlated single-photon counting system that demonstrated the contrast enhancement of>2.5-fold of polyethyle neimine-coated 8T-UCNPs take n up by huma nbreast adeno carcinoma cells *** a result,discrete 8T-UCNP nan oparticles became clearly observable in the freshly excised splee ntissue of laboratory mice 15-min post in trave nous injectio n of an 8T-UCNP *** dem on strated approach paves the way forhigh-contrast,high-resoluti on,and high-speed multiphot on microscopy in challe nging envir onments of i ntense autofluorescence,exogenous staining,and turbidity,as typically occur in intravital imaging.

关键词： upconversion nanoparticles autofluorescence time-gated imaging scanning microscopy time-correlated single photon counting

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THE EXPERIMENTAL STUDY OF STRESS-RELATED PATHOLOGICAL CHANGES IN CEREBRAL vENOUS BLOOD FLOW IN NEWBORN RATS ASSESSED BY DOCT

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Journal of Innovative Optical Health Sciences 2013年第3期6卷 76-85页

作者： OXANA v.SEMYACHKINA-GLUSHKOvSKAYA vLADISLAv v.LYCHAGOv OLGA A.BIBIKOvA IGOR A.SEMYACHKIN-GLUSHKOvSKIY sergey S.SINDEEv EKATERINAM.ZINCHENKO MOHHANAD M.KASSIM AL-FATLE FATEMA ALI AL HASSANI LEITH MARIA v.ULANOvA vALERY v.TUCHIN Department of Biology Saratov State UniversityAstrachanskaya Str.83410012 SaratovRussian Federation Department of Biology Al-Mustansiriya UniversityBaghdadIraq Institute of Precise Mechanics and Control of Russia Academy of Science Rabochaya 28 410028 SaratovRussian Federation Optoelectronics and Measurement Techniques Laboratory University of Oulu P.O.BOX 450090014 University of OuluFinland

In experiments on newbom rats with stress related intracrani al hemorhage(ICH)using Doppler optical coberence tomognaphy(DOCT)we have shown that latent stage of ICH(4 h after stress)is characterized by decrease of venous blood outflow and the loss of sensitivity of sagittal vein to vasoconstrictor ffect of *** incidence of ICH(24 h after stress)was accompanied by progressi on of early pathological changes in cerebral venous blood flow(CvBF)and development of venous *** into consideration of this fact,we suggest that the suppression of CvBF related to the severity to the deleterious effect of stress on the brain hemodynamics in newborn *** facts allow us to conclude that the venous insufficiency with the loss of vasoconstrictor response to adrenaline is an informative and sensitive component of pattern of CvBF that can be important diagnostic criteria of risk of ICH development in newborns.

关键词： Doppler optical coherence tomography stress related intracnanial hemorthage cerebral venous blood flow adrenaline

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Enigmatic Glass-Like Carbon from the Alpine Foreland, Southeast Germany: A Natural Carbonization Process

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Acta Geologica Sinica(English Edition) 2018年第6期92卷 2179-2200页

作者： Tatyana G. SHUMILOvA sergey I. ISAENKO vasily v. ULYASHEv Boris A. MAKEEv Michael A. RAPPENGLüCK Aleksey A. vELIGZHANIN Kord ERNSTSON Institute of Geology of Komi Scientific Center of Ural Branch of Russian Academy of Sciences Pervomayskaya st.54Syktyvkar167982Russia Hawaii Institute of Geophysics and Planetology University of Hawaii at Manoa1680 East-West RoadHonoluluHI96822USA Institute for Interdisciplinary Studies GilchingGermany National Research Center 《Kurchatov Institute》 Akademika Kurchatova pl.1Moscow123182Russia Faculty of Philosophy I University of WürzburgGermany

Unusual carbonaceous matter, termed here chiemite, composed of more than 90% C from the Alpine Foreland at Lake Chiemsee in Bavaria, southeastern Germany has been investigated using optical and atomic force microscopy, X-ray fluorescence spectroscopy, scanning and transmission electron microscopy, high-resolution Raman spectroscopy, X-ray diffraction and differential thermal analysis, as well as by δ13 C and 14 C radiocarbon isotopic data analysis. In the pumice-like fragments, poorly ordered carbon matter co-exists with high-ordering monocrystalline α-carbyne, and contains submicrometersized inclusions of complex composition. Diamond and carbyne add to the peculiar mix of matter. The required very high temperatures and pressures for carbyne formation point to a shock event probably from the recently proposed Holocene Chiemgau meteorite impact. The carbon material is suggested to have largely formed from heavily shocked coal, vegetation like wood, and peat from the impact target area. The carbonization/coalification high PT process may be attributed to a strong shock that instantaneously caused the complete evaporation and loss of volatile matter and water, which nevertheless preserved the original cellular structure seen fossilized in many fragments. Relatively fresh wood encapsulated in the purported strongly shocked matter point to quenched carbon melt components possibly important for the discussion of survival of organic matter in meteorite impacts, implying an astrobiological relationship.

关键词： astrobiology diamond carbyne coalification carbonization meteorite impact chiemite

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