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Functional Interaction of the SNARE Protein NtSyp121 in Ca^2＋ Channel Gating, Ca^2＋ Transientsand ABA Signalling of Stomatal Guard Cells

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Molecular Plant 2008年第2期1卷 347-358页

作者： sergei sokolovski Adrian Hills Robert A. Gay Michael R. Blatt Laboratory of Plant Physiology and Biophysics IBLS--Plant Sciences University of Glasgow Glasgow G12 8QQ UK Current address: Blackfriars 64 St Giles Oxford OX1 3LY UK

There is now growing evidence that membrane vesicle trafficking proteins, especially of the superfamily of SNAREs, are critical for cellular signalling in plants. Work from this laboratory first demonstrated that a soluble, inhibitory （dominant-negative） fragment of the SNARE NtSyp121 blocked K^＋ and CI^- channel responses to the stress-related hormone abscisic acid （ABA）, but left open a question about functional impacts on signal intermediates, especially on Ca^2＋-mediated signalling events. Here, we report one mode of action for the SNARE mediated directly through alterations in Ca^2＋ channel gating and its consequent effects on cytosolic-free [Ca^2＋] （[Ca^2＋]i） elevation. We find that expressing the same inhibitory fragment of NtSyp121 blocks ABA-evoked stomatal closure, but only partially suppresses stomatal closure in the presence of the NO donor, SNAP, which promotes [Ca^2＋]i elevation independently of the plasma membrane Ca^2＋ channels. Consistent with these observations, Ca^2＋ channel gating at the plasma membrane is altered by the SNARE fragment in a manner effective in reducing the potential for triggering a rise in [Ca^2＋]i, and we show directly that its expression in vivo leads to a pronounced suppression of evoked [Ca^2＋]i transients. These observations offer primary evidence for the functional coupling of the SNARE with Ca^2＋ channels at the plant cell plasma membrane and, because [Ca^2＋]i plays a key role in the control of K^＋ and CI^- channel currents in guard cells, they underscore an important mechanism for SNARE integration with ion channel regulation during stomatal closure.

关键词： Ca^2+ channel, hyperpolarization-activated abscisic acid membrane vesicle traffic cytosolic-free [Ca^2+]elevation Nicotiana plant pathogen defense.

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Active and passive modulation of solar light transmittance in a uniquely multifunctional dual-band single molecule for smart window applications

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Journal of Energy Chemistry 2024年第1期88卷 293-305,I0007页

作者： Pooja V.Chavan Pramod V.Rathod Joohyung Lee sergei V.Kostjuk Hern Kim Department of Energy Science and Technology Environmental Waste Recycle InstituteMyongji UniversityYonginGyeonggi-do 17058Republic of Korea Department of Chemical Engineering Myongji UniversityYonginGyeonggi-do 17058Republic of Korea Research Institute for Physical Chemical Problems of the Belarusian State University MinskBelarus

Functional materials may change color by heat and electricity separately or simultaneously in smart *** materials have not only demonstrated remarkable potential in the modulation of solar radiation but are also leading to the development of indoor environments that are more comfortable and conducive to improving individuals'quality of ***,dual-responsive materials have not received ample research attention due to economic and technological *** a consequence,the broader utilization of smart windows faces *** address this new generational multistimulus responsive chromic materials,our group has adopted a developmental strategy to create a poly(NIPAM)n-HV as a switchable material by anchoring active viologen(HV)onto a phase-changing poly(NIPAM)n-based smart material for better utility and *** constructed smart windows facilitate individualistic reversible switching,from a highly transparent state to an opaque state(thermochromic)and a red state(electrochromic),as well as facilitate a simultaneous dual-stimuli response reversible switching from a clear transparent state to a fully opaque(thermochromic)and orange(electrochromic)*** privacy can be attained in smart windows designed for exclusive settings by achieving zero *** unique chromic mode operates independently and modulates visible and near-infrared(NIR)light in a distinct ***,these smart windows with thermal and electric dual-stimuli responsiveness demonstrate remarkable heat regulation capabilities,rendering them highly attractive for applications in building facades,energy harvesting,privacy protection,and color display.

关键词： Smart windows Thermochromism Electrochromism Energy saving Dual-responsive material

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Central Asia revealed as a key area in evolution of Eremurus (Asphodelaceae)

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Plant Diversity 2024年第3期46卷 333-343页

作者： Dilmurod Makhmudjanov sergei Volis Ziyoviddin Yusupov Inom Juramurodov Komiljon Tojibaev Tao Deng Hang Sun CAS Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Yunnan International Joint Laboratory for Biodiversity of Central Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina Institute of Botany Academy Sciences of UzbekistanTashkent 100125Uzbekistan University of Chinese Academy of Sciences Beijing 100864China

Eremurus was described at the beginning of the 19th ***,due to limited sampling and the small number of gene markers to date,its phylogeny and evolution are largely *** this study,we analyzed plastomes from 27 species belonging to 2 subgenera and 3 sections of Eremurus,which are found in Central Asia(its center of diversity)and *** also analyzed nuclear DNA ITS of 33 species,encompassing all subgenera and sections of the genus in Central Asia,southwest Asia and *** findings revealed that the genus was monophyletic,although both subgenera Eremurus and Henningia were found to be *** plastome and nrDNA-based phylogenetic trees had three clades that did not reflect the current taxonomy of the *** biogeographical and time-calibrated trees suggest that Eremurus originated in the ancient Tethyan area in the second half of the *** of Eremurus occurred from the early Oligocene to the late *** Sea retreat and several orogenetic events,such as the progressive uplift of the Qinghai-Tibet Plateau and surrounding mountain belts(Altai,Pamir,Tian Shan),caused serious topographic and climate(aridification)changes in Central Asia that may have triggered a split of clades and *** this transformed Central Asia,speciation proceeded rapidly driven mainly by vicariance caused by numerous mountain chains and specialization to a variety of climatic,topographic and soil conditions that exist in this region.

关键词： Asphodelaceae Asphodeloideae Plastome Central Asia Qinghai-Tibet Plateau

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Impact of doping profiles on the formation of laser-active centers in bismuth-doped GeO_(2)–SiO_(2)glass fibers

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Photonics Research 2024年第2期12卷 260-270页

作者： SERGEY ALYSHEV ALEXANDER VAKHRUSHEV ALEKSANDR KHEGAI ELENA FIRSTOVA KONSTANTIN RIUMKIN MIKHAIL MELKUMOV LYUDMILA ISKHAKOVA ANDREY UMNIKOV sergei FIRSTOV Prokhorov General Physics Institute of the Russian Academy of Sciences Dianov Fiber Optics Research Center119333 MoscowRussia Devyatykh Institute of Chemistry of High-Purity Substances of the Russian Academy of Sciences 603951 Nizhny NovgorodRussia

Multi-wavelength-band transmission technology based on the exploitation of the extended spectral region is considered as a potential approach to increase the transmission capacity in the deployed fiber-optic communication *** development of optical amplifiers operating in the O-,E-,S-,and U-telecom bands is an extremely important challenge for the successful implementation of this ***-doped fibers are of increasing interest as gain materials,which can be used to provide broadband amplification in the mentioned telecom *** is due to the ability of Bi ions incorporated into glass network to form bismuth active centers(BACs)with specific optical properties,which are primarily determined by the glass *** this work,the impact of the doping profiles of both Ge atoms as glass modifiers and Bi ions on the BACs formation is studied using a series of bismuth-doped fibers fabricated by the modified chemical vapor deposition(MCVD)*** Bi-to-BACs conversion efficiency in various spatial regions of the studied samples is *** is turned out that for high-Bi concentration regions,the conversion efficiency is very low(less than 10%).In addition,the relationship of the conversion efficiency to the distribution of Bi ions and/or Ge atoms is ***,a continuous-wave laser at 1.46μm with a record slope efficiency of 80%is demonstrated using a Bi-doped fiber with confined doping profile,where the Bi-to-BACs conversion efficiency is 35%.This paper provides new information which might help to facilitate understanding of the features of Bi-doped fibers and their potentially achievable characteristics.

关键词： fiber fibers bismuth

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Theory and Properties of Atomic Spacetime

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Journal of Applied Mathematics and Physics 2024年第6期12卷 1967-2006页

作者： sergei Yu. Eremenko Department of Mathematical Modeling and Artificial Intelligence National Aerospace University Kharkiv Aviation Institute Kharkiv Ukraine Soliton Scientific Sydney Australia

Following A. Einsteins aspirations for an atomic theory, a novel theory of spacetime quantization/atomization based on finite Atomic AString Functions evolving since the 1970s is offered. Atomization Theorems allow representing polynomials, analytic functions, and solutions of General Relativity via the superposition of solitonic atoms which can be associated with flexible spacetime quanta, metriants, or elementary distortions. With multiple interpretations discussed, discrete-continuous spacetime is conceptualized as a lattice network of flexible solitonic atoms adjusting locations to reproduce different metrics. The theory may offer some variants of unified field theory under research based on Atomic AString Function where, like in string theory, fields become interconnected having a common mathematical ancestor.

关键词： Spacetime Quantum Atomic Function AString Soliton Metriant Unified Theory

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Large-scale error-tolerant programmable interferometer fabricated by femtosecond laser writing

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Photonics Research 2024年第3期12卷 I0028-I0040页

作者： ILYA KONDRATYEV VERONIKA IVANOVA SUREN FLDZHYAN ARTEM ARGENCHIEV NIKITA KOSTYUCHENKO SERGEY ZHURAVITSKII NIKOLAY SKRYABIN IVAN DYAKONOV MIKHAIL SAYGIN STANISLAV STRAUPE ALEXANDER KORNEEV sergei KULIK Quantum Technology Centre and Faculty of Physics M.V.Lomonosov Moscow State UniversityMoscow 119991Russia Russian Quantum Center Moscow 121205Russia Laboratory of Quantum Engineering of Light South Ural State UniversityChelyabinsk 454080Russia

We introduce a programmable eight-port interferometer with the recently proposed error-tolerant architecture capable of performing a broad class of *** interferometer has been fabricated with femtosecond laser writing,and it is the largest programmable interferometer of this kind to *** have demonstrated its advantageous error tolerance by showing an operation in a broad wavelength range from 920 to 980 nm,which is particularly relevant for quantum photonics due to efficient photon sources existing in this wavelength *** work highlights the importance of developing novel architectures of programmable photonics for information processing.

关键词： interferometer laser femtosecond

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Prognostic and predictive role of immune microenvironment in colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 643-652页

作者： Olesya Kuznetsova Mikhail Fedyanin Larisa Zavalishina Larisa Moskvina Olga Kuznetsova Alexandra Lebedeva Alexey Tryakin Galina Kireeva Gleb Borshchev sergei Tjulandin Ekaterina Ignatova Department of Chemotherapy Federal State Budgetary Institution(N.N.Blokhin National Medical Research Center of Oncology)of the Ministry of Health of the Russian FederationMoscow 115478Russia Department of Pathology Russian Medical Academy of Continuous Professional EducationMoscow 123242Russia OncoAtlas LCC Moscow 115478Russia Federal State Budgetary Institution“National Medical and Surgical Center named after N.I.Pirogov”of the Ministry of Health of the Russian Federation Moscow 105203Russia STOONCO:Science and Technology in Oncology Moscow 115478Russia

Colorectal cancer(CRC)represents a molecularly heterogeneous disease and one of the most frequent causes of cancer-related death *** traditional classification of CRC is based on pathomorphological and molecular character-istics of tumor cells(mucinous,ring-cell carcinomas,etc.),analysis of mechanisms of carcinogenesis involved(chromosomal instability,microsatellite instability,CpG island methylator phenotype)and mutational statuses of commonly altered genes(KRAS,NRAS,BRAF,APC,etc.),as well as expression signatures(CMS 1-4).It is also suggested that the tumor microenvironment is a key player in tumor progression and metastasis in *** to the latest data,the immune microenvironment can also be predictive of the response to immune checkpoint *** this review,we highlight how the immune environment influences CRC prognosis and sensitivity to systemic therapy.

关键词： Immunoscore Immune microenvironment Colorectal cancer Gastrointestinal cancers Predictive biomarkers Digital pathology

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Precise mode control of laser-written waveguides for broadband,low-dispersion 3D integrated optics

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Light(Science & Applications) 2024年第6期13卷 1186-1195页

作者： Yuying Wang Lijing Zhong Kuen Yao Lau Xuhu Han Yi Yang Jiacheng Hu sergei Firstov Zhi Chen Zhijun Ma Limin Tong Kin Seng Chiang Dezhi Tan Jianrong Qiu College of Optical Science and Engineering Zhejiang University310027HangzhouChina Institute of Light+X Science and Technology College of Information Science and EngineeringNingbo University315211NingboChina School of Optoelectronic Science and Engineering&Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University215006SuzhouChina Prokhorov General Physics Institute of the Russian Academy of Sciences Dianov Fiber Optics Research Center38 Vavilov str.Moscow119333Russia Zhejiang Lab 311121HangzhouChina College of Materials Science and Engineering Key Laboratory of Advanced Materials of Yunnan ProvinceKunming University of Science and Technology650093KunmingYunnanChina Department of Electrical Engineering City University of Hong Kong83 Tat Chee AvenueKowloonHong Kong SARChina School of Materials Science and Engineering Zhejiang University310027HangzhouChina

Three-dimensional(3D)glass chips are promising waveguide platforms for building hybrid 3D photonic circuits due to their 3D topological capabilities,large transparent windows,and low coupling *** present,the key challenge in scaling down a benchtop optical system to a glass chip is the lack of precise methods for controlling the mode field and optical coupling of 3D waveguide ***,we propose an overlap-controlled multi-scan(OCMS)method based on laser-direct lithography that allows customizing the refractive index profile of 3D waveguides with high spatial precision in a variety of *** the basis of this method,we achieve variable mode-field distribution,robust and broadband coupling,and thereby demonstrate dispersionless LP21-mode conversion of supercontinuum pulses with the largest deviation of<0.1 dB in coupling ratios on 210 nm *** approach provides a route to achieve ultra-broadband and low-dispersion coupling in 3D photonic circuits,with overwhelming advantages over conventional planar waveguide-optic platforms for on-chip transmission and manipulation of ultrashort laser pulses and broadband supercontinuum.

关键词： waveguide mode dispersion

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Optimizing ASReview Simulations:A generic Multiprocessing Solution for“Light-data'and“Heavy-data'Users

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Data Intelligence 2024年第2期6卷 320-343页

作者： sergei Romanov Abel Soares Siqueira Jonathan de Bruin Jelle Teijema Laura Hofstee Rens van de Schoot Applied Data Science Department of Information and Computing ScienceFaculty of ScienceUtrecht University Netherlands eScienceCenter AmsterdamNL Department of Research and Data Management Services Information Technology ServicesUtrecht UniversityUtrechtthe Netherlands Department of Methodology and Statistics Faculty of Social and Behavioral SciencesUtrecht University

Active learning can be used for optimizing and speeding up the screening phase of systematic *** simulation studies mimicking the screening process can be used to test the performance of different machine-learning models or to study the impact of different training *** paper presents an architecture design withamultiprocessing computational strategyforrunningmanysuch simulation studiesinparallel,using the ASReview Makita workflow generator and Kubernetes software for deployment with cloud *** provide a technical explanation of the proposed cloud architecture and its *** addition to that,we conducted 1140 simulations investigating the computational time using various numbers of CPUs and RAM *** analysis demonstrates the degree to which simulations can be accelerated with multiprocessing computing *** parallel computation strategy and the architecture design that was developed in the present paper can contribute to future research with more optimal simulation time and,at the same time,ensure the safe completion of the needed processes.

关键词： Active learning Systematic review Simulation study Multiprocessing Cloud architecture

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Spatial analysis of vegetation cover response to climate trends in Khakassia(South Siberia)

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Journal of Mountain Science 2023年第10期20卷 2869-2884页

作者： IM sergei Institute of Natural Sciences and Mathematics Khakassian State University named after N.F.KatanovAbakan 655000Russia V.N.Sukachev Institute of Forest Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of ScienceKrasnoyarsk 660036Russia Institute of Ecology and Geography Siberian Federal UniversityKrasnoyarsk 660041Russia Institute of Space Research and High Technologies Reshetnev Siberian State University of Science and TechnologyKrasnoyarsk660014Russia

An analysis of the changes in vegetation cover on the territory of the Republic of Khakassia in 2000–2021 due to climatic trends was carried out based on the MODIS *** changes in vegetation cover were estimated based on trends in Normalized Difference Vegetation Index(NDVI)and Enhanced Vegetation Index(EVI).In general,in the 21st century,an increase in the biomass of vegetation cover is *** trends were observed in 16%–22%of the territory,and negative only in 1%–3%.For about 20%of the analyzed territory,a significant influence of climate on the changes in vegetation cover was *** most pronounced negative impact on vegetation cover was caused by summer air and soil temperatures,spring temperature,and summer winds,and the positive impact was caused by summer precipitation and soil *** response of the vegetation cover to climate was non-uniform concerning the ***,a significant correlation with the amount of precipitation was observed for~20%–35%of vegetation growing below 600 m above sea level and for less than 5%above this *** negative effect of summer temperatures on plants prevailed mainly at an elevation below~1400 m above sea *** climate change is likely to lead to significant degradation of vegetation in the steppe and foreststeppe in Khakassia in the coming decades.

关键词： Khakassia South Siberia Vegetation cover Climate trends Remote sensing MODIS NDVI EVI Relief

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