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The Basic Helix-Loop-Helix Transcription Factor MYC1 Is Involved in the Regulation of the Flavonoid Biosynthesis Pathway in Grapevine

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Molecular Plant 2010年第3期3卷 509-523页

作者： Imene Hichri Simon C. Heppel Jeremy Pillet Celine Leon Stefan Czemmel serge delrot Virginie Lauvergeat Jochen Bogs Institut des Sciences de la Vigne et du Vin UMR 1287 Ecophysiologie et G~nomique Fonctionnelle de la Vigne~lNRA Universit~ de Bordeaux 210 Chernin deLeysotte 33882 Villenave d'Ornon France Heideiberger Institut for Pflanzenwissenschaften im Neuenheimer Feld 360 69120 Heidelberg Germany

Previous results indicated that in grapevine （Vitis vinifera）, regulation of the flavonoid pathway genes by MYB transcription factors depends on their interaction with basic helix-loop-helix proteins （bHLHs）. The present study describes the first functional characterization of a bHLH factor from grapevine named VvMYC1. This transcription factor is phylogenetically related to Arabidopsis bHLH proteins, which participate in the control of flavonoid biosynthesis and epidermal cell fate. Transient promoter and yeast two-hybrid assays demonstrated that VvMYC1 physically interacts with MYB5a, MYB5b, MYBA1/A2, and MYBPA1 to induce promoters of flavonoid pathway genes involved in anthocyanin and/ or proanthocyanidin （PA） synthesis. Additionally, transient promoter assays revealed that VvMYC1 is involved in feedback regulation of its own expression. Transcript levels of VvMYC1 during berry development correlate with the synthesis of anthocyanins and PAs in skins and seeds of berries, suggesting that VvMYC1 is involved in the regulation of anthocyanins and PA synthesis in these organs. Likewise, transient expression of VvMYC1 and VvMYBA1 induces anthocyanin synthesis in grapevine suspension cells. These results suggest that VvMYCl is part of the transcriptional cascade controlling antho- cyanin and PA biosynthesis in grapevine.

关键词： Fruit ripening molecular physiology secondary metabolismmphenylpropanoids and phenolics gene regu-lation transcriptional control and transcription factors grapevine.

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Long-distant transport of glutathione by OsGT1

Long-distant transport of glutathione by OsGT1

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第十三届全国植物基因组学大会

作者： serge delrot Univ.Bordeaux IsvvEcophysiologie Et Genomique Fonctionnelle De La VigneUmr 1287F33140Villenave D’ornon 0France

Glutathione(GSH) is an essential metabolite in protecting cells against oxidative damages under normal and stress *** could be intercellular transported and long-distant transported in the ***1 is a GSH transporter,bu...

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Proteomic and metabolomic profiling underlines the stage- and time-dependent effects of high temperature on grape berry metabolism

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Journal of Integrative Plant Biology 2020年第8期62卷 1132-1158页

作者： David Lecourieux Christian Kappel Stéphane Claverol Philippe Pieri Regina Feil John E.Lunn Marc Bonneu Lijun Wang Eric Gomès serge delrot Fatma Lecourieux UMR1287 EGFV INRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance Institut of Biochemistry and Biology Potsdam UniversityD-14476 PotsdamGermany Proteome Platform Bordeaux Functional Genomic CenterBordeaux University33076 BordeauxFrance Max Planck Institute of Molecular Plant Physiology 14476 PotsdamGolmGermany Institute of Botany the Chinese Academy of SciencesBeijing 100093China UMR1287 EGFV CNRSINRAEBordeaux Sciences AgroBordeaux UniversityISVV33140 Villenave d'OrnonFrance

Climate change scenarios predict an increase in mean air temperatures and in the frequency,intensity,and length of extreme temperature events in many wine-growing regions *** elevated temperature has detrimental effects on berry growth and composition,it threatens the economic and environmental sustainability of wine *** Cabernet Sauvignon fruit-bearing cuttings,we investigated the effects of high temperature(HT)on grapevine berries through a label-free shotgun proteomic analysis coupled to a complementary metabolomic *** the 2,279 proteins identified,592 differentially abundant proteins were found in berries exposed to *** gene ontology categories“stress,”“protein,”“secondary metabolism,”and“cell wall”were predominantly altered under *** temperatures strongly impaired carbohydrate and energy metabolism,and the effects depended on the stage of development and duration of *** amounts correlated poorly with protein expression levels in HT berries,highlighting the value of proteomic studies in the context of heat ***,this work reveals that HT alters key proteins driving berry development and ***,we provide a list of differentially abundant proteins that can be considered as potential markers for developing or selecting grape varieties that are better adapted to warmer climates or extreme heat waves.

关键词： metabolism extreme high

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水稻谷胱苷肽转动蛋白基因(OsGT1)的分离和鉴定

水稻谷胱苷肽转动蛋白基因(OsGT1)的分离和鉴定

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中国植物学会七十周年年会

作者：张明永麦维军王颖 serge delrot 中国科学院华南植物研究所法国普瓦捷大学

谷胱苷肽(GSH)是植物及其他生物体内主要的还原态硫之一(Leusteketal,2000)。GSH是一个三肽,并仅在叶绿体及胞浆中合成,它具有多方面的功能:如还原态硫的贮存及转运形式、调控含硫化合物的合成、胞内氧化还原态的控制、在生物与环境胁...

谷胱苷肽(GSH)是植物及其他生物体内主要的还原态硫之一(Leusteketal,2000)。GSH是一个三肽,并仅在叶绿体及胞浆中合成,它具有多方面的功能:如还原态硫的贮存及转运形式、调控含硫化合物的合成、胞内氧化还原态的控制、在生物与环境胁迫中起着保护作用、调节蛋白折叠、细胞分裂循环等(May等1998;Foyer等2001)。叶盘及原生质体实验表明,植物中存在GSH转运系统,而且是H调节的质子共转运(Jamai et al.,1996)。这里报道第一个植物GSH转运蛋白基因的分离与功能鉴定。

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水稻谷胱苷肽转运蛋白(OsGI1)启动子的分离和鉴定

水稻谷胱苷肽转运蛋白(OsGI1)启动子的分离和鉴定

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中国遗传学会第七次代表大会暨学术讨论会

作者：王颖麦维军张明永梁承邺 serge delrot 中国科学院华南植物研究所法国普瓦捷大学

谷胱苷肽(GSH)是在γ-谷氨酰半胱氨酸合成酶及谷胱苷肽合成酶的催化作用下,通过两个依赖ATP提供能量的反应,由谷氨酸、半胱氨酸和甘氨酸合成的。它具有多方面的功能,如:贮藏和转运还原态硫,调控含硫化合物的合成,调节细胞内的氧化还原态...

谷胱苷肽(GSH)是在γ-谷氨酰半胱氨酸合成酶及谷胱苷肽合成酶的催化作用下,通过两个依赖ATP提供能量的反应,由谷氨酸、半胱氨酸和甘氨酸合成的。它具有多方面的功能,如:贮藏和转运还原态硫,调控含硫化合物的合成,调节细胞内的氧化还原态,防御生物和非生物的胁迫,以及参与控制蛋白的折叠和细胞分裂循环等(May等1998;Foyer等2001)。最近,水稻谷胱苷肽转运蛋白(OsGT1)首次成功得到分离,将OsGT1构建到酵母表达载体

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The role of auxin during early berry development in grapevine as revealed by transcript profiling from pollination to fruit set

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Horticulture Research 2021年第1期8卷 1924-1938页

作者： Francisca Godoy Nathalie Kuhn Mindy Muñoz German Marchandon Satyanarayana Gouthu Laurent Deluc serge delrot Virginie Lauvergeat Patricio Arce-Johnson Departamento de Genetica Molecular y Microbiologia Facultad de Ciencias BiologicasPontificia Universidad Catolica de ChileAlameda 340SantiagoChile Facultad de Ciencias Agronomicas y de los Alimentos Pontificia Universidad Catolica de Valparaiso2340025 ValparaisoChile Department of Horticulture Oregon State UniversityCorvallisOR 97331USA UMR Ecophysiologie et Genomique Fonctionnelle de la Vigne ISVVUniversitéde BordeauxVillenave d´OrnonFrance

Auxin is a key phytohormone that modulates fruit formation in many fleshy fruits through the regulation of cell division and *** content rapidly increases after pollination and the manipulation in its levels may lead to the parthenocarpic *** Vitis vinifera L.,little is known about the early fruit development that encompasses from pollination to fruit ***/fertilization events trigger fruit formation,and auxin treatment mimics their effect in grape berry ***,the role of auxin in this process at the molecular level is not well *** elucidate the participation of auxin in grapevine fruit formation,morphological,reproductive,and molecular events from anthesis to fruit set were described in sequential days after *** RNA-seq analysis at four time points from anthesis to fruit set revealed that the highest percentage of genes induced/repressed within the hormone-related gene category were auxin-related *** profiling showed significant transcript variations in auxin signaling and homeostasis-related genes during the early fruit *** acetic acid and several auxin metabolites were present during this ***,application of an inhibitor of auxin action reduced cell number and the mesocarp diameter,similarly to unpollinated berries,further confirming the key role of auxin during early berry *** work sheds light into the molecular features of the initial fruit development and highlights the auxin participation during this stage in grapevine.

关键词： development. auxin fir

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Water limitation and rootstock genotype interact to alter grape berry metabolism through transcriptome reprogramming

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Horticulture Research 2015年第1期2卷 242-254页

作者： Mariam Berdeja Philippe Nicolas Christian Kappel Zhan Wu Dai Ghislaine Hilbert Anthony Peccoux Magali Lafontaine Nathalie Ollat Eric Gomès serge delrot Ecophysiologie et Génomique Fonctionnelle de la Vigne University BordeauxISVVUMR 1287Villenave d′Ornon F-33140France Department of General and Organic Viticulture Hochschule Geisenheim UniversityGeisenheimGermany Boyce Thompson Institute for Plant Research IthacaNY 14850USA Institut für Biochemie und Biologie Universität PotsdamKarl-Liebknecht-Str.24–25Potsdam 14476Germany

Grapevine is a perennial crop often cultivated by grafting a scion cultivar on a suitable *** influence scions,particularly with regard to water uptake and ***,one of the possibilities to adapt viticulture to the extended drought stress periods is to select rootstocks conferring increased tolerance to ***,the molecular mechanisms associated with the ability of rootstock/scion combination to influence grape berry metabolism under drought stress are still poorly *** transcriptomic changes induced by drought stress in grape berries(*** noir)from vines grafted on either 110R(drought-tolerant)or 125AA(drought-sensitive)rootstock were *** experiments were conducted in the vineyard for two years and two grape berry developmental stages(50%and 100%veraison).The genome-wide microarray approach showed that water stress strongly impacts gene expression in the berries,through ontology categories that cover cell wall metabolism,primary and secondary metabolism,signaling,stress,and hormones,and that some of these effects strongly depend on the rootstock ***,under drought stress,berries from vines grafted on 110R displayed a different transcriptional response compared to 125AA-concerning genes related to jasmonate(JA),phenylpropanoid metabolism,and pathogenesis-related *** data also suggest a link between JA and secondary metabolism in water-stressed ***,genes related to secondary metabolism and JA are more induced and/or less repressed by drought stress in the berries grafted on the drought-sensitive rootstock *** rootstock-dependent gene expression changes are relevant for berry composition and sensory properties.

关键词： metabolism drought programming

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