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A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells

Microsystems & Nanoengineering 2019年第1期5卷 593-605页

作者： Yue Yu Richard P.Sde Campos seolim hong Dimitar L.Krastev Siddharth Sadanand Yen Leung Aaron R.Wheeler Institute for Biomaterials and Biomedical Engineering University of Toronto164 College StTorontoON M5s 3G9Canada Donnelly Centre for Cellular and Biomolecular Research 160 College St.TorontoON M5S 3E1Canada Department of Chemistry University of Toronto80 St George St.TorontoON M5S 3H6Canada Department of Human Biology University of Toronto300 Huron StreetTorontoON M5S 3J6Canada

Homeostasis of dopamine,a classical neurotransmitter,is a key indicator of neuronal *** in the regulation of dopamine is implicated in a long list of neurological disorders,including addiction,depression,and *** existing methods used to evaluate dopamine homeostasis in vitro are inconvenient and do not allow for continuous non-destructive *** response to this challenge,we introduce an integrated microfluidic system that combines dopaminergic cell culture and differentiation with electroanalytical measurements of extracellular dopamine in real-time at any point during an *** used the system to examine the behavior of differentiated SH-SY5Y cells upon exposure to four dopamine transporter ant/agonists(cocaine,ketamine,epigallocatechin gallate,and amphetamine)and study their *** IC_(50)values of cocaine,ketamine,and epigallocatechin gallate were determined to be(average±standard deviation)3.7±1.1μM,51.4±17.9μM,and 2.6±0.8μM,***,we used the new system to study amphetamine-mediated dopamine release to probe the related phenomena of dopamine transporter-mediated reverse-transport and dopamine release from *** propose that this platform,which is the first platform to simultaneously evaluate uptake and release,could be useful to screen for drugs and other agents that target dopaminergic neurons and the function of the dopamine *** broadly,this platform should be adaptable for any application that could benefit from high-temporal resolution electroanalysis combined with multi-day cell culture using small numbers of cells.

关键词： dopamine homeostasis release

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Correction to:A microfluidic platform for continuous monitoring of dopamine homeostasis in dopaminergic cells

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Microsystems & Nanoengineering 2019年第1期5卷 514-514页

Correction to:Microsystems&Nanoengineering(2019)5:10 https://***/10.1038/s41378-019-0049-2 published online:11 March 2019 The legend in Fig.4a in the previously published version of this article contained erroneous *** correct units are given in the caption–that is,1μM(black),500 nM(red),100 nM(blue),50 nM(cyan),10 nM(pink),and 0 nM(1×PBS solution,brown).

关键词： homeostasis brown monitoring

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