检索结果-南通市图书馆

Effect of PDS and lycopene on learning and memory of senile mice cumulating with lactic acid in atlanto-axial joint

在线全文

学校读者我要写书评

暂无评论

Effect of PDS and lycopene on learning and memory of senile ...

肾上腺素对脂多糖诱导的小鼠巨噬细胞炎症介质表达的影响

2008 International Conference on Ginseng

作者： FAN Wen-jing ZHU Tong-tong GUO Ya-xiong LIN Gui-miao ZHAO Li-juan Department of Pathophysiology school of basic medicine sciences Jilin University Changchun 130021

＜正＞Objective To compare PDS with lycopene in the function of the improvement of learning and memory abilities by cumulating lactic acid for long time between atlas dentata vertebrae in senile *** 30% lactic acid 30μL one time every week was injected into the place between the first and the second cervical vertebrae,three times was *** changes of learning and memory in mice were observed with water-maze *** activities of acetylcholine esterase（ChE）, and the content of acetylcholine,（ACh）in encephalon were tested,Immunohistochemical staining withβ-amyloid protein in mouse brain,including the cortex and hippocampus was *** The model mice had longer swimming full time and more error times（p＜0.05）in water maze test compared with control *** could significantly shortened the latency period and wrong times in water-maze test（P＜0.01）,but lycopene’mice showing was not *** with model group,the activities of ChE increased（P＜0.01）,and the content of ACh decreased in the PDS and lycopene group（P＜0.01）,and in the two therapy groups,β-amyloid protein in mouse brain of cortex and hippocampus was less than the model ***:Our findings indicate that PDS and lycopene has improved the learning and memory abilities,as well as its ability to ameliorate the metabolism in senile mice encephalon, which may be the mechanism of its cerebral protective effect.

关键词： PDS lycopene learning and memory atlanto-axial joint β-amyloid

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

华中科技大学学报（医学版） 2004年第5期33卷 538-540页

作者：张力叶笃筠吴萍万敬员张代娟吴涛周小燕华中科技大学同济医学院基础医学院病理生理学系武汉430030

目的　研究肾上腺素对脂多糖 (LPS)诱导的小鼠单核巨噬细胞株RAW 2 6 4 7中肿瘤坏死因子 (TNF α)、一氧化氮 (NO)、环加氧酶 2 (COX 2 )等表达的影响。方法　以 10ng/ml的LPS刺激体外培养的RAW 2 6 4 7细胞作为炎症模型 ,加入不同浓... 详细信息

目的　研究肾上腺素对脂多糖 (LPS)诱导的小鼠单核巨噬细胞株RAW 2 6 4 7中肿瘤坏死因子 (TNF α)、一氧化氮 (NO)、环加氧酶 2 (COX 2 )等表达的影响。方法　以 10ng/ml的LPS刺激体外培养的RAW 2 6 4 7细胞作为炎症模型 ,加入不同浓度的肾上腺素 (1× 10 -6,5× 10 -6,1× 10 -5,5× 10 -5mol/L)孵育 2 4h后 ,收集培养上清并提取细胞总蛋白 ,酶联免疫法测定上清中TNF α浓度 ,Griess法检测上清NO含量 ,免疫印迹法检测细胞总蛋白中COX 2的表达量。结果　 10ng/ml的LPS明显诱导TNF α、NO、COX 2的产生 ,这一效应可被肾上腺素抑制 ;而单独加入肾上腺素时 ,对上述 3种炎症介质的表达无明显影响。结论　肾上腺素下调LPS诱导的巨噬细胞中炎症因子的表达 ,可能对炎症的发生发展起到一定的调节作用。

关键词：肾上腺素表达量炎症介质 COX-2 TNF-α 诱导巨噬细胞细胞总蛋白一氧化氮(NO) 体外培养

Acute pancreatitis as a complication of acute COVID-19 in kidney transplant recipients

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第6期12卷 1104-1110页

作者： Nikolina basic-Jukic Ivana Juric Lea Katalinic Vesna Furic-Cunko Vibor Sesa Anna Mrzljak Department of Nephrology Arterial HypertensionDialysis and TransplantationUniversity Hospital Centre ZagrebZagreb 10000Croatia Department of medicine School of MedicineZagreb 10000Croatia Department of Gastroenterology and Hepatology University Hospital Centre ZagrebZagreb 10000Croatia

BACKGROUND Acute pancreatitis is a rare extrapulmonary manifestation of coronavirus disease 2019(COVID-19)but its full correlation with COVID-19 infection remains *** To identify acute pancreatitis’occurrence,clinical presentation and outcomes in a cohort of kidney transplant recipients with acute *** A retrospective observational single-centre cohort study from a transplant centre in Croatia for all adult renal transplant recipients with a functioning kidney allograft between March 2020 and August 2022 to record cases of acute pancreatitis during acute *** were obtained from hospital electronic medical *** acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection was proven by a positive SARS-CoV-2 real-time reverse transcriptase-polymerase chain reaction on the nasopharyngeal *** Four hundred and eight out of 1432(28.49%)patients who received a renal allograft developed COVID-19 *** analyzed cohort included 321 patients(57%males).One hundred and fifty patients(46.7%)received at least one dose of the anti-SARS-CoV-2 vaccine before the *** hundred twenty-five(39.1%)patients required hospitalization,141(44.1%)developed pneumonia and four patients(1.3%)required mechanical *** included immunosuppression modification in 233 patients(77.1%)and remdesivir in 53 patients(16.6%),besides the other supportive *** the study cohort,only one transplant recipient(0.3%)developed acute pancreatitis during acute COVID-19,presenting with abdominal pain and significantly elevated pancreatic *** survived without complications with a stable kidney allograft *** Although rare,acute pancreatitis may complicate the course of acute COVID-19 in kidney transplant *** mechanism of injury to the pancreas and its correlation with the severity of the COVID-19 infection in kidney transplant recipients warrants further research.

关键词： Acute pancreatitis COVID-19 Kidney transplant Angiotensin-converting enzyme-2 receptor Immunosuppressive agents

Microstructure and Wear Characteristics of Al-4.5Cu-5Pb Alloy

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 1998年第2期14卷 125-131页

作者： Om Prakas Pandey(school of basic and Applied sciences, Thapar Institute of Engineering & Technology, Patiala-147001, India) school of basic and Applied sciences Thapar Institute of Engineering and Technology Patiala-147001 India

Al-4. 5Cu-5Pb alloy was prepared by sand and chill casting. The same alloy was also spray deposited at a gas pressure of 1.6 MPa. The microstructural features exhibit a coarse to fine dendritic morphology for sand and chill cast alloys. Equiaxed grains were observed for spray formed alloys. Wear testing employing a pin-on-disc type set-up, reveals considerably lower wear of spray deposited alloy compared to that of chill and sand cast alloys. The morphological features of wear track on specimen and debris indicated a mixed oxidative-cum-adhesive wear mechanisms for these alloys tested in the present investigation. (Edited author abstract) 36 Refs.

关键词： Al Cu Microstructure and Wear Characteristics of Al-4.5Cu-5Pb Alloy Pb

Altered subcellular distribution of nucleolar protein fibrillarin by actinomycin D in HEp-2 cells

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第7期25卷 56-60页

作者： Min CHEN2, Ping JIANG Research Center of basic medicine, school of medicine, Jiangsu University, Zhenjiang 212001, China Research Center of basic medicine School of Medicine Jiangsu University Zhenjiang 212001 Chin

AIM: To study the effects of actinomycin D on subcellular distribution of nucleolar protein fibrillarin in HEp-2 (human esophageal epithelial type 2) cells, and molecular mechanisms for maintenance of fibrillarin in nucleolus. METHODS: Indirect immunofluorescence assay was employed to investigate subcellular distribution of nucleolar protein fibrillarin and immunoblotting analysis was used to detect the total cellular amount of fibrillarin. RESULTS: Control cells with no drug treatment showed bright clumpy nucleolar staining, which indicated that fibrillarin decorated the nucleolus only. Treatment with actinomycin D caused dislocation of fibrillarin from nucleoli to nucleoplasm with numerous stained small nucleoplasmic entities. Immunoblotting showed that neither total cellular amount of fibrillarin nor the integrity of fibrillarin was changed upon the treatment. The dislocation of fibrillarin in cells treated at a lower concentration (0.05 mg/L) of actinomycin D, was totally reversible after removal of the drug from the medium. However, this reversion was not observed at a high drug concentration (1 mg/L). CONCLUSION: Actinomycin D induced dislocation of fibrillarin from nucleoli to nucleoplasm in HEp-2 cells. The retention of fibrillarin within the nucleolus was related to active RNA synthesis.

关键词： dactinomycin nuclear proteins fluorescence antibody technique immunoblotting

β2 Adrenergic receptor on T lymphocytes and its clinical implications

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2009年第1期19卷 17-23页

作者： Xuelai Fan a,Yuedan Wang b,a Peking University,school of Pharmaceutical sciences,Beijing 100083,China b Peking University,school of basic Medical sciences,Beijing 100083,China [a]Peking University School of Pharmaceutical Sciences Beijing 100083 China [b]Peking University School of Basic Medical Sciences Beijing 100083 China

Sustained complex cross-talk between the immune system and the nervous system plays a vital role in retaining homeostasis in a healthy *** of the central regulatory mechanisms involved is the existence and functions of β2-adrenergic receptors(β2AR) on T *** article reviews research progress made recently,including the expression of adrenergic receptors on T lymphocytes,the structure and intracellular pathways of β2AR,the activation of β2AR by either endogenous or exogenous agonists,and the effect of β2AR stimulation on T cells which alters T cell proliferation,differentiation,cytokine production and T-helper-mediated antibody ***,we discuss the roles of β2AR played in the pathogenesis and treatment of autoimmune diseases.

关键词： Neuroimmunology β2-adrenergic receptor T cell Autoimmune

Distinct Pattern of Human Vδ1 γδ T Cells Recognizing MICA

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2005年第4期2卷 253-258页

作者： Jianqiang Li~1 Lianxian Cui~1 Wei He~(1,2) ~1Department of Immunology,Institute of basic Medical sciences,Chinese Academy of Medical sciences and school of basic medicine,Peking Union Medical College,5 Dong Dan San Tiao,Beijing 100005,China Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and School of Basic Medicine Peking Union Medical College Beijing 100005 China.

γδT cells represent one unique recognition pattern,the limited recognition,which distinguishes from the specificrecognition for αβT cells and pattern recognition for macrophages.Vδ1 γδT cell is the major subset of human γδT cells,which predominates in mucosal tissue including the intestinal ***,a few antigens thathuman Vδ1TCR can recognize have been *** them,MHC class I chain-related molecules A (MICA)have been studied most *** Vδ1TCR,MICA is also the ligand of NKG2D,a C-type lectin-likeactivating *** human,only Vδ1 cells can simultaneously express both types of receptors of MICAwhile NK cells,αβT cells and other subsets of γδT cells likewise express *** the precisemechanisms are still enigmatic,this distinct pattern of Vδ1 cells recognizing MICA predicts unique biologicalsignificance of Vδ1 cells in immune *** years,some progresses have been made in this *** thisreview we summarize the related reports and put forward some novel views based on our group’s ***& Molecular Immunology.2005;2(4):253-258.

关键词： Vδ1TCR MICA NKG2D γδ T cell

A novel erythroid differentiation related gene EDRF2 inhibited α-globin gene expression in K562 cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2002年第5期47卷 398-402页

作者： WANG Duncheng, YANG Xiaoming & SHEN BeifenInstitute of basic Medical sciences, Institute of Radiation medicine sciences, Academy of Military sciences, Beijing 100850, China Institute of basic Medical sciences Institute of Radiation Medicine Sciences Academy of Military Sciences Beijing China

In previous studies, we found that EDRF2 was an erythroid differentiation related factor, whose expression was markedly upregulated during erythroid differentiation. It suggested that this factor played a role in erythropoiesis. By using rapid amplification of cDNA ends technology, we cloned EDRF2 gene 5’-and 3’-cDNA ends successfully. Transfection and Northern blot analysis demonstrated that EDRF2 inhibited mRNA expression of a-globin gene, while did not regulate γ-globin gene expression. Gel shift assay confirmed that DNA-binding activity of erythroid specific transcription factors GATA-1, NF-E2 and AP1 was not affected by either forced overexpression or artificial down-regulation of EDRF2 gene in K562 cells. However, we detected that the negative regulator of expression of GATA-1 transcription factor was increased in EDRF2 overexpressed K562 cells. These results strongly suggested that EDRF2 was involved in a-globin gene expression and erythroid differentiation and served as a negative regulator of

关键词： globin gene erythroid specific transcription factors leucine zipper RACE EMSA.

Genitourinary tumors and liver transplantation: A comprehensive review

在线全文

学校读者我要写书评

暂无评论

World Journal of Transplantation 2024年第3期14卷 52-58页

作者： Vibor Sesa Hrvoje Silovski Nikolina basic-Jukic Iva Kosuta Maja Sremac Anna Mrzljak Department of Gastroenterology and Hepatology University Hospital Center ZagrebZagreb 10000Croatia Department of Hepatobiliary Surgery and Transplantation University Hospital Center ZagrebZagreb 10000Croatia Department of medicine School of MedicineZagreb 10000Croatia Department of Nephrology Arterial hypertensionDialysis and TransplantationUniversity Hospital Center ZagrebZagreb 10000Croatia Department of Intensive Care medicine University Hospital Center ZagrebZagreb 10000Croatia

Liver transplantation is as a crucial therapeutic option for patients with end-stage liver disease,but the persistent organ shortage emphasizes a need to explore unconventional donor sources,including individuals with a history of *** review investigates the viability of liver donation from individuals with current or past genitourinary malignancies,focusing on renal,prostate and urinary bladder *** rising incidence of urogenital malignancies among potential donors is thought to result from increasing donor *** of transmission risks reveals low rates of donor-derived cancer transmission,partic-ularly for early-stage renal and prostate *** with a history of genitourinary malignancy pose complex challenges regarding post-transplant immunosuppression and cancer ***,the evidence suggests acceptable outcomes can be achieved with careful patient selection and tailored management *** for pre-transplant evaluation and post-transplant surveillance are discussed,highlighting the need for individualized approaches in this patient *** prospective studies are warranted to refine guidelines and optimize outcomes in liver transplantation for patients with genitourinary malignancies.

关键词： Liver transplantation Genitourinary malignancies Donor-transmitted cancer Malignancy recurrence Immunosuppression