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Discovery of the inhibitor of DNA binding 1 as a novel marker for radioresistance in pancreatic cancer using genome-wide RNA-seq

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Cancer Drug Resistance 2022年第4期5卷 926-938页

作者： scar zuniga Stephanie Byrum Adam R.Wolfe Department of Radiation Oncology University of Arkansas for Medical SciencesLittle RockAR 72205USA Department of Biochemistry and Molecular Biology University of Arkansas for Medical SciencesLittle RockAR 72205USA

Purpose/Objective(s): Discovery of genetic drivers of radioresistance is critical for developing novel therapeuticstrategies to combine with radiotherapy of radioresistant PDAC. In this study, we used genome-wide RNA-seq toidentify genes upregulated in generated radioresistant PDAC cell lines and discovered the Inhibitor of DNA Binding1 (ID1) gene as a potential regulator of radioresistance in ***/Methods: Radioresistant clones of the PDAC cell lines MIA PaCa-2 and PANC-1 were generated bydelivering daily ionizing irradiation (IR) (2 Gy/day) in vitro over two weeks (total 20 Gy) followed by standardclonogenic assays following one week from the end of IR. The generated RR and parental cell lines were submittedfor RNA-seq analysis to identify differentially expressed genes. The Limma R package was used to calculatedifferential expression among genes. Log2 fold change values were calculated for each sample compared to thecontrol. Genes with an absolute fold change > 1 were considered significant. RNA sequencing expression data fromthe Cancer Genome Atlas (TCGA) database was analyzed through the online databases GEPIA, cBioPortal, and theHuman Protein ***: Following exposure to two weeks of 2 Gy daily IR in vitro, the two PDAC cell lines showed significantlygreater clonogenic cell survival than their parental cell lines, indicating enhanced RR in these cells. RNA-seqanalysis comparing parental and RR cell lines found upregulated seven genes (TNS4, ZDHHC8P1, APLNR, AQP3,SPP1, ID1, ID2) and seven genes downregulated (PTX3, ITGB2, EPS8L1, ALDH1L2, KCNT2, ARHGAP9, IFI16) in bothRR cell lines. Western blotting confirmed increased expression of the ID1 protein in the RR cell lines compared totheir parental cell lines. We found that ID1 mRNA was significantly higher in PDAC tumors compared to matchednormal and high ID1 expression correlated with significantly worse disease-free survival (DFS) in PDAC patients(HR = 2.2, log rank P = 0.009). ID1 mRNA express

关键词： Pancreatic cancer radiation resistance ID1 RNA-seq

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The Effects of Laser Therapy in Treating Hypertrophic scars and Keloids after Median Sternotomy:A Scoping Review

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Congenital Heart Disease 2024年第4期19卷 363-374页

作者： Laura Schianchi Fabrizio Vaira Massimo Chessa Serena Francesca Flocco Arianna Magon Gianluca Conte Karina Geraldina zuniga Olaya Giacomo Bortolussi Erika Cioffi Matteo Riccardo Di Nicola Santo Raffaele Mercuri Rosario Caruso Adult Congenital Heart Disease Unit IRCCS Policlinico San DonatoSan Donato MilaneseMilan20097Italy Dermatology and Cosmetology Unit IRCCS Ospedale San RaffaeleMilan20132Italy Faculty of Medicine and Surgery Vita-Salute San Raffaele UniversityMilan20132Italy Health Professions Research and Development Unit IRCCS Policlinico San DonatoSan Donato MilaneseMilan20097Italy Department of Congenital Cardiac Surgery IRCCS Policlinico San DonatoSan Donato MilaneseMilan20097Italy Cardiac Surgery IRCCS Policlinico San DonatoSan Donato MilaneseMilan20097Italy Department of Biomedical Sciences for Health University of MilanMilan20133Italy

Background:Hypertrophic scars and keloids,common complications following median sternotomy for cardiac surgery,signiﬁcantly impact patient quality of life due to their aesthetic and symptomatic *** advancements in laser therapy have made it a prominent option for managing these complex scars,yet a com-prehensive understanding of its efﬁcacy is *** aim of this scoping review is to explore the effects of laser therapy in managing hypertrophic scars and keloids after median ***:This scoping review ana-lyzed studies up to February 2024 from databases including PubMed,EMBASE,CINAHL,Scopus,Web of Science,and the Cochrane *** included any study that assessed laser therapy’s effects on hypertrophic scars and keloids following median *** were selected based on predeﬁned inclusion criteria with-out publication year,design,or origin ***:Six studies met the inclusion criteria,involving a total ofﬁve RCTs and one *** studies primarily tested 585 and 595-nm pulsed dye laser(PDL)treatments,focusing on scar appearance,patient symptoms,and treatment *** studies reported signiﬁcant improvements in scar height reduction and patient symptom relief after treatment,with mixed results for scar erythema and *** events were generally mild and ***:Laser therapy offers a beneﬁcial approach for improving the appearance and symptoms of hypertrophic scars and keloids post-median ***,further research is necessary to optimize treatment parameters and explore the long-term psychosocial impacts of this *** review highlights the need for more comprehensive studies to establish standardized treatment protocols and evaluate their effectiveness.

关键词： Laser therapy hypertrophic scars keloids median sternotomy cardiac surgery pulsed dye laser scar management

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