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Serological diagnosis of nasopharyngeal carcinoma by enzyme linked immunosorbant assay:optimization,standardization and diagnostic criteria

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chinese Medical Journal 1998年第6期 51-56页

作者： M.H. Ng, H.l. chen, R.X. luo, K.H. chan, P.c.Y. woo, J.S.T. Sham, J. Huang, W.H.Seto P. Smith and B.E. Griffin

Objectives To produce an enzyme linked immunosorbant assay (ElISA) to detect antibodies against Epstein Barr virus (EBV) specified nuclear antigen 1 (EBNA 1) and the 18kD EBV matrix protein, and to determine and optimize its sensitivity and specificity for the diagnosis of nasopharyngeal carcinoma (NPc).Methods We used a combination of highly purified glutathione transferase fusion proteins of the 40kD carboxy domain of EBNA1 and the 18kD EBV matrix protein for coating ElISA plates. In three separate studies, we tested for IgA antibodies in serum specimens from 28 EBV seronegative donors, 284 EBV seropositive donors and 160 newly diagnosed NPc patients. By comparing the sensitivity and specificity of diagnosis obtained for different cutoff values, we derived several quantitative parameters to evaluate assay performance, establish objective diagnostic criteria which optimize the intrinsic diagnostic capability of the assay and assess the significance of individual test results, respectively. Optimum cutoff optical density (OD) is defined as the cutoff OD where sensitivity of the assay equals its specificity, and resolution of the assay is indicated by the value of sensitivity (or specificity) determined at the optimum cutoff OD. Diagnosis of NPc was achieved by setting a cutoff zone at +/-20% of this *** All the EBV seronegative donors tested were not reactive, and most of the EBV seropositive donors were weakly reactive, while the majority of NPc patients were moderately or strongly reactive. While the assay was thus shown to be specific for EBV, there was an overlap in the level of these serum antibodies between few individuals of the two latter groups. It was shown that the assay performed equally well in two separate studies conducted under different testing conditions and using different collections of sera in that assay resolution determined on these occasions were 86% and 87% respectively. Diagnosis of NPc can be achieved at the same expected sensiti

关键词： Serological diagnosis of nasopharyngeal carcinoma by enzyme linked immunosorbant assay

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平行轴齿轮传动系统布局设计的膨胀方法

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机械工程学报 2001年第12期37卷 16-20页

作者：陆一平查建中 Tony c woo 北方交通大学机电学院北京100044 University of Washington

布局设计是齿轮传动系统设计中重要的核心问题之一，它的关键在于建立一种有效的问题表　达和求解模型。提出使用膨胀布局方法来解决这一问题。膨胀方法是一种通过构造布局物体　的膨胀和排斥而自适应地求解物体布局位置的计算方法，能... 详细信息

布局设计是齿轮传动系统设计中重要的核心问题之一，它的关键在于建立一种有效的问题表　达和求解模型。提出使用膨胀布局方法来解决这一问题。膨胀方法是一种通过构造布局物体　的膨胀和排斥而自适应地求解物体布局位置的计算方法，能够表达齿轮传动的多层结构和复　杂啮合约束，是较好的齿轮传动系统布局求解模型。

关键词：齿轮传动 cAD 膨胀布局设计

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Factors that contribute to the mycoparasitism stimulus in Trichoderma atroviride. strain P1

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浙江大学学报(农业与生命科学版) 2004年第4期30卷 421-421页

作者： woo S l Formisano E Fogliano V cosenza c Mauro A Turrà D Soriente I Ferraioli S Scala F lorito M Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica and Dip. Di Scienza degli Alimenti Università di Napoli "Federico II" Via Università 100 80055 Portici (NA) Italy.

Trichoderma atroviride strain P1 has been used extensively to study the mycoparasitic mechanisms in the interaction between plant pathogenic host and beneficial antagonistic fungi. Mutants of P1 containing the green fluorescent protein (gfp) or glucose oxidase (gox) reporter systems and different inducible promoters (from the exochitinase nag1 gene, or the endochitinase ech42 gene of P1) were used to determine the factors that activate the biocontrol gene expression cascade in the antagonist. The following compounds were tested singly and in various combinations: purified Trichoderma P1 enzymes (endochitinase, exochitinase, chitobiosidase, glucanase); antagonist culture filtrates (T. atroviride P1 wild-type and relative knock-out mutants, T. harzianum, T. reesei); pathogen culture filtrates (Botrytis, Pythium, Rhizoctonia); purified fungal cell walls (cWs) from Trichoderma, Botrytis, Pythium, Rhizoctonia; colloidal crab shell chitin; and plant extracts from cucumber leaves, stems or roots. Strong induction of mycoparasitism was found with the various digestion products produced by treating fungal cWs and colloidal chitin with purified enzymes or fungal culture filtrates. Filtrates from chitinase knock-out mutants, as well as cWs from Oomycetes fungi, were less active in producing the stimulus for mycoparasitism. The host cW digestion products were separated by molecular weight (MW) to determine which compounds were able to activate Trichoderma. Micromolecules of MW less than 3 kDa were found to trigger mycoparasitism gene expression before physical contact with the host pathogen. These compounds stimulated mycelial growth and spore germination of the antagonist. Purification of these host-derived compounds was conducted by HPlc and in vivo assay. The obtained inducers were able to stimulate both the production of endochitinase and exochitinase enzymes, even under repressing conditions in the presence of glucose. Inducers stimulated the biocontrol effect of P1 in the

关键词： fungal cell walls hydrolytic enzymes mycoparasitism inducers

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Utilizing hybrid faradaic mechanism via catalytic and surface interactions for high-performance flexible energy storage system

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能源化学:英文版 2023年第8期83卷 541-548,I0013页

作者： Dong-Gyu lee Hyeonggeun choi Yeonsu Park Min-cheol Kim Jong Bae Park Suok lee Younghyun cho wook Ahn A-Rang Jang Jung Inn Sohn John Hong Young-woo lee Department of Energy Engineering Soonchunhyang UniversityAsan 31538Republic of Korea Division of Physics and Semiconductor Science Dongguk University-SeoulSeoul 04620Republic of Korea Jeonju centre Korea Basic Science InstituteJeonju 54907Republic of Korea Division of Electrical Electronic and Control EngineeringKongju National UniversityCheonan 31080Republic of Korea School of Materials Science and Engineering Kookmin UniversitySeoul 02707Republic of Korea

Improving the capacitance and energy density is a significant challenge while developing practical and flexible energy storage system(ESS).Redox mediators(RMs),as redox-active electrolyte additives,can provide additional energy storing capability via electrochemical faradaic contribution on electrodes for high-performance flexible ***,determining effective material combinations between electrodes and RMs is essential for maximizing surface faradaic redox reactions for energy-storage *** this study,an electrode-RM system comprising heterostructured hybrid(carbon fiber(cF)/MnO_(2)) faradaic electrodes and iodine RMs(I-RMs) in a redox-active electrolyte is *** cF/MnO_(2)with the 1-RMs(cF/MnO_(2)-I) induces dominant catalytic faradaic interaction with the I-RMs,significantly enhancing the surface faradaic kinetics and increasing the overall energy-storage *** cF/MnO_(2)-I ESSs show a 12.6-fold(or higher) increased volumetric energy density of 793.81 mWh l^(-1)at a current of 10 μA relative to ESSs using cF/MnO_(2)without I-RMs(cF/MnO_(2)).Moreover,the cF/MnO_(2)-I retains 93.1% of its initial capacitance after 10,000 cycles,validating the excellent ***,the flexibility of the ESSs is tested at different bending angles(180° to 0°),demonstrating its feasibility for flexible and high-wear ***,cF/MnO_(2)electrodes present a practical material combination for high-performance flexible energy-storage devices owing to the catalytic faradaic interaction with I-RMs.

关键词： Energy storage system Redox mediators Faradaic electrodes catalytic interactions Mechanical stability

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Engineering Geometric Electrodes for Electric Field-Enhanced High-Performance Flexible In-Plane Micro-Supercapacitors

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Energy & Environmental Materials 2023年第4期6卷 114-120页

作者： Jihong Kim Sung Min Wi Jong-Guk Ahn Sangjun Son Hee Young lim Yeonsu Park Hye Ji Eun Jong Bae Park Hyunseob lim Sangyeon Pak A-Rang Jang Young-woo lee Department of Energy Engineering Soonchunhyang UniversityAsan 31538Korea Department of chemistry Gwangju Institute of Science and TechnologyGwangju 61005Korea Jeonju centre Korea Basic Science InstituteJeonju 54907Korea Prof.S.Pak School of Electronic and Electrical Engineering Hongik UniversitySeoul 04066Korea Division of Electrical Electronic and Control EngineeringKongju National UniversityCheonan 31080Korea

In plane micro-supercapacitors that are miniaturized energy storage components have attracted significant attention due to their high power densities for various ubiquitous and sustainable device systems as well as their facile integration on various flexible/wearable *** implement the micro-supercapacitors in various practical applications that can accompany solid state or gel electrolyte and flexible substrates,ions must be readily transported to electrodes for achieving high power ***,we show large enhancement in electrochemical properties of flexible,inplane micro-supercapacitor using sharp-edged interdigitated electrode design,which was simply fabricated through direct laser scribing *** sharp-edged electrodes allowed strong electric field to be induced at the corners of the electrode fingers which led to the greater accumulation of ions near the surface of electrode,significantly enhancing the energy storage performance of *** electric field-enhanced in-plane micro-supercapacitor showed the volumetric energy density of 1.52 Wh l^(−1)and the excellent cyclability with capacitive retention of 95.4%after 20000 *** further showed various practicability of our sharp-edged design in micro-supercapacitors by showing circuit applicability,mechanical stability,and air *** results present an important pathway for designing electrodes in various energy storage devices.

关键词： electric field enhancement flexible energy storage device microsupercapacitors sharp electrodes

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Use of Ultrasound Bath in the Extraction and Quantification of Ester-linked Phenolic Acids in Tropical Forages

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American Journal of Analytical chemistry 2011年第3期2卷 344-351页

作者： Mellina Damasceno Rachid Santos Aline de Paula Vitor Jailton da costa carneiro Domingos savio campos Paciullo Renato camargo Matos Maria Auxiliadora costa Matos NUPIS(Nucleo de Pesquisa em Instrumentacao e Separacao Analiticas) Departamento de QuimicaInstituto de Ciencias ExatasUniversidade Federal de Juiz de ForaJuiz de ForaBrazil EMBRAPA—centro Nacional de Pesquisa de Gado de leite(cNPGl) Juiz de ForaBrazil

A method was developed for the analysis of ester-linked phenolic acids in forage samples using extraction by an ultrasound-assisted treatment and quantification by HPlc with a UV-VIS detector. A reversed-phase c18 column was used for developing the method and the optimal condition was established with isocratic elution using acetonitrile/methanol/H3PO4 pH 2.08 (13:12.5:74.5) as the mobile phase. To reduce the time of sample processing, the extraction of ester-linked phenolic acids was studied using ultrasound bath and the results were then compared with those from an extraction usual using alkaline hydrolysis (20°c for 24 h). The method was valued through external and internal calibration. Internal calibration using o-coumaric acid as internal standard and m-coumaric acid as surrogate internal standard showed better results. The detection limits were of 0.09 and 0.04 mg●l-1 for p-coumaric and ferulic acids, respectively. The proposed method showed a good linear dynamic range (3.00 - 30.00 mg●l-1) for the analytes. The usefulness of the methodology was demonstrated by addition-recovery experiments using forage samples and values were in the 83 to 99% range. The extraction of ester-linked phenolic acids by 120 minutes of ultrasound bath was faster and more reproducible than alkaline hydrolysis (20°c for 24 h).

关键词： Ultrasound Bath Phenolic Acids Forage Internal calibration

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Saeng-Kankunbi-Tang(生肝健脾汤) Protects liver against Oxidative Damage through Activation of ERK/Nrf2 Pathway

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chinese Journal of Integrative Medicine 2016年第8期22卷 619-628页

作者： In woo lee Hee Yoon choi Ju-Hee lee Sun-Dong Park Seung Mo Kim Sae Kwang Ku 赵容杰 Sang chan Kim Young woo Kim Hong Sik choi Department of Internal Oriental Medicine of Hepatology College of Oriental Medicine Daegu Haany University Daegu 38610 Korea Medical Research center for Globalization of Herbal Formulation College of Oriental Medicine Daegu Haany University Daegu 38610 Korea college of Oriental Medicine Dongguk University Gyeongju 38066 Korea School of Mental Health Jining Medical University JiningShandong Province 272067 China

Objective： To investigate the cytoprotective effects of Saeng-kankunbi-tang（生肝健脾汤, SKT）, a herbal prescription consisting of Artemisia capillaris and Alisma canaliculatum, and its underlying mechanism involved. Methods： In mice, blood biochemistry and histopathology were assessed in carbon tetrachloride（ccl4）-induced oxidative hepatic injury in vivo. The animal groups included vehicle-treated control, ccl4, SKT 500 mg/（kg·day） ccl4＋SKT 200 or 500 mg/（kg·day）. In Hep G2 cell, tert-butyl hydroperoxide（t BHP） induced severe oxidative stress and mitochondrial dysfunction in vitro. The cyto-protective effects of SKT were determined by 3-（4,5-dimethylthiazol）-2,5-diphenyltetrazolium bromide（MTT） assay, fluorescence activated cell sorting analysis and western blotting. Results： The administration of SKT prevented liver damage induced by ccl4 in mice, by inhibition of hepatocyte degeneration and inflammatory cell infiltration as well as plasma parameters such as alanine aminotransferase（P〈0.01）. Moreover, treatment with t BHP induced hepatocyte death and cellular reactive oxygen species production in hepatocyte cell line. However, SKT pretreatment（30–300 μg/m l） reduced this cell death and oxidative stress（P〈0.01）. More importantly, SKT inhibited the ability of t BHP to induce changes in mitochondrial membrane transition in cell stained with rhodamine 123（P〈0.01）. Furthermore, treatment with SKT induced extracellular signal-regulated kinases-mediated nuclear factor erythroid-2-related factor 2（Nrf2） activation as well as the expressions of heme oxygenase 1 and glutamate-cystein ligase catalytic, Nrf2 target genes. conclusion： SKT has the ability to protect hepatocyte against oxidative stress and mitochondrial damage mediated by Nrf2 activation.

关键词： Saeng-kankunbi-tang mitochondria nuclear factor erythroid-2-related factor 2 oxidative stress liver mouse

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Proteomic study of three component interactions: plant, pathogens and antagonistic fungi

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浙江大学学报(农业与生命科学版) 2004年第4期30卷 449-449页

作者： Marra R Ambrosino P Scala V Romano c Vinale F Ferraioli S Ruocco M carbone V woo S l Turrà D Scala F lorito M Dip. Arboricoltura Botanica e Patologia Vegetale-Sez. Patologia Vegetale Lab. di Lotta Biologica Università di Napoli "Federico II" Via Università 100 80055 Portici (NA)Italy ISA-cNR Avellino.

The molecular factors involved in the three-way interaction between plant, pathogenic fungi and antagonistic/biocontrol fungi, such as Trichoderma, are still poorly understood, even if they represent a matter of interest for improving crop management and developing new strategies for plant diseases control. The aim of this work is to investigate the components involved in this interaction and, for this purpose, a proteomic approach was used. 2-D maps of the protein extracts from the single components in various interactions between plants (potato, bean, tobacco or tomato), pathogens (Botrytis cinerea, Rhizoctonia solani or Pythium ultimum) and biocontrol fungi (Trichoderma atroviride strain P1 or Trichoderma harzianum strain T22) were obtained. The proteome of each partner was collected separately and extracted by acetone precipitation in presence of trichloroacetic acid and a reducing agent (DTT). The extracted proteins were separated by isoelectrofocusing (IEF), using IPG (Immobilized pH gradient) strips, followed by SDS-PAGE. In order to improve resolution the separations were performed both on wide than narrow pH range and on different gel lengths. Differential spots were noted in the proteome of the three-way interaction when compared to each single component. These were further characterized by mass spectrometry and in silico analysis with the aim of identifying and cloning the relative genes. During the in vitro interaction of T. harzianum strain T22 with tomato and the culture filtrate or cell walls of pathogens, the spot number was higher than in the presence of pathogen biomass. In terms of Trichoderma differential proteins displayed on 2D gels, the most important changes were obtained in the presence of P. ultimum . During the in vivo interaction with tomato, the antagonist proteome changed much more in presence of soilborne fungi R. solani and P. ultimum than with the foliar fungus B. cinerea, both in terms of total and increased or novel spots. In silic

关键词： differential proteins in vivo interactions induced disease resistance

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Recent advances and applications of deep learning methods in materials science

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npj computational Materials 2022年第1期8卷 548-573页

作者： Kamal choudhary Brian Decost chi chen Anubhav Jain Francesca Tavazza Ryan cohn cheol woo Park Alok choudhary Ankit Agrawal Simon J.l.Billinge Elizabeth Holm Shyue Ping Ong chris Wolverton Materials Science and Engineering Division National Institute of Standards and TechnologyGaithersburgMD20899USA Theiss Research La JollaCA92037USA DeepMaterials llc Silver SpringMD20906USA Material Measurement Science Division National Institute of Standards and TechnologyGaithersburgMD20899USA Department of NanoEngineering University of California San DiegoSan DiegoCA92093USA Energy Technologies Area Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Materials Science and Engineering Carnegie Mellon UniversityPittsburghPA15213USA Department of Materials Science and Engineering Northwestern UniversityEvanstonIL60208USA Department of Electrical and computer Engineering Northwestern UniversityEvanstonIL60208USA Department of Applied Physics and Applied Mathematics and the Data Science Institute Fu Foundation School of Engineering and Applied SciencesColumbia UniversityNew YorkNY10027USA

Deep learning(Dl)is one of the fastest-growing topics in materials data science,with rapidly emerging applications spanning atomistic,image-based,spectral,and textual data *** allows analysis of unstructured data and automated identification of *** recent development of large materials databases has fueled the application of Dl methods in atomistic prediction in *** contrast,advances in image and spectral data have largely leveraged synthetic data enabled by high-quality forward models as well as by generative unsupervised Dl *** this article,we present a high-level overview of deep learning methods followed by a detailed discussion of recent developments of deep learning in atomistic simulation,materials imaging,spectral analysis,and natural language *** each modality we discuss applications involving both theoretical and experimental data,typical modeling approaches with their strengths and limitations,and relevant publicly available software and *** conclude the review with a discussion of recent cross-cutting work related to uncertainty quantification in this field and a brief perspective on limitations,challenges,and potential growth areas for Dl methods in materials science.

关键词： learning limitations textual

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Thick free-standing electrode based on carbon-carbon nitride microspheres with large mesopores for high-energy-density lithium-sulfur batteries

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carbon Energy 2021年第3期3卷 410-423页

作者： Hui-Ju Kang Tae-Gyu lee Heejin Kim Jae-woo Park Hyun Jin Hwang Hyeonseok Hwang Kwang-Suk Jang Hae Jin Kim Yun Suk Huh Won Bin Im Young-Si Jun Department of Advanced chemicals&Engineering Chonnam National UniversityBuk-guGwangjuRepublic of Korea Division of Analytical Science Korea Basic Science Institute(KBSI)Yuseong-guDaejeonRepublic of Korea School of chemical Engineering Chonnam National UniversityBuk-guGwangjuRepublic of Korea Department of Applied chemistry Hanyang UniversityAnsanGyeonggi-doRepublic of Korea Department of Biological Engineering Inha UniversityNam-guIncheonRepublic of Korea Division of Materials Science and Engineering Hanyang UniversitySeongdong-guSeoulRepublic of Korea

The development of sulfur cathodes with high areal capacity and high energy density is crucial for the practical application of lithium-sulfur batteries(lSBs).lSBs can be built by employing(ultra)high-loading sulfur cathodes,which have rarely been realized due to massive passivation and ***,microspheres of a carbon-carbon nitride composite(c@cN)with large mesopores are fabricated via molecular cooperative *** the c@cN-based electrodes,the effects of the large mesopores and N-functional groups on the electrochemical behavior of sulfur in lSB cells are thoroughly investigated under ultrahigh sulfur-loading conditions(>15 mgS cm^(-2)).Furthermore,for high-energy-density lSBs,the c@cN powders are pelletized into a thick free-standing electrode(thickness:500^m;diameter:11 mm)via a simple briquette process;here,the total amount of energy stored by the lSB cells is 39 mWh,corresponding to a volumetric energy density of 440 Wh l-1 with an areal capacity of 24.9 and 17.5 mAh cm^(-2) at 0.47 and 4.7 mA cm^(-2),respectively(at 24mgS cm^(-2)).These results have significantly surpassed most recent records due to the synergy among the large mesopores,(poly)sulfide-philic surfaces,and thick *** developed strategy with its potential for scale-up successfully fills the gap between laboratory-scale cells and practical cells without sacrificing the high areal capacity and high energy density,providing a solid foundation for the development of practical lSBs.

关键词： briquette process carbon nitride free-standing electrode high energy density lithium-sulfur batteries mesopores

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