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Association of colorectal cancer with pathogenic Escherichia coli: Focus on mechanisms using optical imaging

World Journal of Clinical Oncology 2016年第3期7卷 293-301页

作者： Julie Veziant Johan Gagnière Elodie Jouberton Virginie Bonnin Pierre sauvanet Denis Pezet Nicolas Barnich Elisabeth Miot-Noirault Mathilde Bonnet UMR1071 Inserm/Université d’Auvergne and INRA USC2018 Clermont Université Service de Chirurgie Digestive Centre Hospitalier Universitaire UMR990 Inserm/Université d’Auvergne Clermont Université Service de Médecine Nucléaire Centre Jean Perrin

AIM: To investigate the molecular or cellular mechanisms related to the infection of epithelial colonic mucosa by pks-positive Escherichia coli(E. coli) using optical ***: We choose to evaluate the tumor metabolic activity using a fluorodeoxyglucose analogue as 2-deoxyglucosone fluorescent probes and to correlate it with tumoral volume(mm^3). Inflammation measuring myeloperoxidase(MPO) activity and reactive oxygen species production was monitored by a bioluminescent(BLI) inflammation probe and related to histological examination and MPO levels by enzyme-linked immunosorbent assay(ELISA) on tumor specimens. The detection and quantitation of these two signals were validated on a xenograft model of human colon adenocarcinoma epithelial cells(HCT116) in nude mice infected with a pks-positive E. coli. The inflammatory BLI signal was validated intra-digestively in the colitisCEABAC10 DSS models, which mimicked Crohn's disease. RESULTS: Using a 2-deoxyglucosone fluorescent probe, we observed a high and specific HCT116 tumor uptake in correlation with tumoral volume(P = 0.0036). Using the inflammation probe targeting MPO, we detected a rapid systemic elimination and a significant increase of the BLI signal in the pks-positive E. coli-infected HCT116 xenograft group(P < 0.005). ELISA confirmed that MPO levels were significantly higher(1556 ± 313.6 vs 234.6 ± 121.6 ng/m L P = 0.001) in xenografts infected with the pathogenic E. coli strain. Moreover, histological examination of tumor samples confirmed massive infiltration of pks-positive E. coli-infected HCT116 tumors by inflammatory cells compared to the uninfected group. These data showed that infection with the pathogenic E. coli strain enhanced inflammation and ROS production in tumors before tumor growth. Moreover, we demonstrated that the intra-digestive monitoring of inflammation is feasible in a reference colitis murine model(CEABAC10/DSS).CONCLUSION: Using BLI and fluorescence optical imaging, we provided

关键词： Colorectal carcinoma Escherichia coli Colibactin Myeloperoxidase In vivo optical imaging

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Laparoscopic fenestration of pancreatic serous cystadenoma:Minimally invasive approach for symptomatic benign disease

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World Journal of Gastroenterology 2015年第22期21卷 7047-7051页

作者： Safi Dokmak Béatrice Aussilhou Fanjandrainy Rasoaherinomenjanahary Alain sauvanet Marie-Pierre Vullierme Vinciane Rebours Philippe Lévy Department of HPB Surgery and Liver Transplantation Beaujon Hospital Department of Radiology Beaujon Hospital Department of Gastroenterology Beaujon Hospital

Serous cystadenoma(SC) is a benign pancreatic cystic tumor. Surgical resection is recommended for symptomatic forms, but laparoscopic fenestration of large symptomatic macrocystic SC was not yet described in the literature. In this study, 3 female patients underwent laparoscopic fenestration for macrocystic SC(12-14 cm). Diagnosis was established via magnetic resonance imaging and endoscopic ultrasound, with intra-cystic dosage of tumors markers(ACE and CA19-9) in 2 patients. All patients were symptomatic and operated on 15-60 mo after *** evaluation showed constant cyst *** were informed about this new surgical modality that can avoid pancreatic resection. The mean operative time was 103 min(70-150 min) with one *** post-operative course was marked by a grade A pancreatic fistula in one patient and was uneventful in the other two. The hospital stay was 3, 10, and 18 d, respectively. The diagnosis of macrocystic SC was histologically-confirmed in all cases. At the last followup(13-26 mo), all patients were symptom-free, and radiological evaluation showed complete disappearance of the cyst. Laparoscopic fenestration, as opposed to resection, should be considered for large symptomatic macrocystic SC, thereby avoiding pancreatic resection morbidity and mortality.

关键词： Pancreatic serous cystadenoma Laparoscopicfenestration Symptomatic

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Role of frozen section assessment for intraductal papillary and mucinous tumor of the pancreas

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World Journal of Gastrointestinal Surgery 2010年第10期2卷 352-358页

作者： Alain sauvanet Anne Couvelard Jacques Belghiti Service de Chirurgie Hépatique et Pancréatique Hpital BeaujonAssistance Publique-Hopitaux de Paris Université Paris Service d'Anatomie Pathologique Hpital BeaujonAssistance Publique-Hopitaux de Paris Université Paris VII

Intraductal papillary mucinous neoplasms(IPMN) of the pancreas include a spectrum of dysplasia ranging from minimal mucinous hyperplasia to invasive carcinoma and are extensive tumors that often spread along the ductal *** studies have demonstrated that preoperative imaging is not accurate enough to adapt the extent of pancreatectomy and have suggested routinely using frozen sectioning(FS) to evaluate the completeness of resection and also to check if ductal dilatation is active or passive,in order to avoid an excessive pancreatic *** main duct and branch duct analysis is needed due to the difference in the natural history of the *** accuracy averages 95%.Eroded epithelium on the main duct,severe ductal inflammation mimicking dysplasia and reactive epithelial changes secondary to obstruction can lead to inappropriate FS *** results change the planned extent of resection in up to 30% of *** optimal cut-off leading to extend pancreatectomy is not consensual and our standard option is to extend pancreatec-tomy if FS reveals:(1) at least IPMN adenoma on the main duct;or(2) at least borderline IPMN on branch ducts;or(3) invasive ***,the decision to extend resection must be taken after a multidisciplinary discussion since it does not exclusively depend on the FS result but also on age,general condition and expected prognosis after *** main limitation of using FS is the existence of discontinuous("skip") lesions which account for approximately 10% of IPMN in surgical series and can lead to reoperation in up to 8% of cases.

关键词： Intraductal papillary and mucinous tumor Pancreas Frozen section Branch duct Dysplasia Main duct

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Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation

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World Journal of Gastroenterology 2014年第21期20卷 6560-6572页

作者： Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud Clermont Université UMR1071 Inserm/Ud’Auvergne and INRA USC201863000 Clermont-FerrandFrance Centre Hospitalier Universitaire 63000 Clermont-FerrandFrance

AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(***)isolated from the colonic mucosa of cancer ***:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated *** from colon cancer and diverticulosis specimens weredetermined by *** and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection *** antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western *** colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic *** proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of ***:Analysis of mucosa-associated *** from colon cancer and diverticulosis specimens showed that whatever the origin of the *** strains,86%of cyclomodulin-positive *** belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)*** vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 *** strains were poorly adherent and ***,mucosa-associated B2 *** similarly to Crohn’s disease-associated *** are able to induce CEACAM6expression in T84 intestinal epithelial *** addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 *** strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell ***:In conclusion,these data bring new insights into the ability of *** isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis.

关键词： B2 Escherichia coli Carcinoembryonic antigen-relat

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A rare cause of obstructive jaundice and gastric outlet obstruction

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World Journal of Gastrointestinal Surgery 2013年第6期5卷 192-194页

作者： Florence Jeune Gaspard d'Assignies Alain sauvanet Sébastien Gaujoux Department of Hepato-Pancreato-Biliary Surgery Ple des Maladies de l'Appareil Digestif(PMAD)AP-HPBeaujon Hospital92110 ClichyFrance Department of Radiology Ple des Maladies de l'Appareil Digestif(PMAD)AP-HPBeaujon Hospital92110 ClichyFrance University Paris 7 Denis Diderot 75013 ParisFrance

Superior mesenteric artery syndrome is a rare cause of upper gastrointestinal obstruction,and is characterized by 3 rd duodenal obstruction between the abdominal aorta and the superior mesenteric *** symptoms are postprandial epigastric pain,nausea,vomiting,and weight loss,or acute upper gastrointestinal *** herein describe an unusual presentation,with jaundice due to compression of the common bile duct by the gastric obstruction and dilated duodenum.

关键词： Upper gastrointestinal obstruction Superior mesenteric artery syndrome Obstructive jaundice

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胰腺术后患者死亡原因分析多中心队列研究(CARE研究)

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肝胆外科杂志 2021年第6期29卷 479-480页

作者： Beugniez C sauvanet a Sulpice L Gaujoux S Turrini O Truant S Schwarz L Piessen G Regimbeau JM Muscari F Adham M Pattou F Schneider L Clement G Delpero JR Pruvot FR Mehdi EA 崔笑(摘译) 薛鸿(摘译) 耿小平(审校) French-ACHBT Working Group 不详

虽然近年来胰腺手术技术取得了长足进步,但胰腺术后发生死亡的事件依然仍有发生.本研究分析了法国多个高流量胰腺外科中心2015年1月至2018年12月期间接受胰腺切除术90天内死亡的原因.共9个中心纳入研究患者3195例,术后90天内死亡140例,3... 详细信息

虽然近年来胰腺手术技术取得了长足进步,但胰腺术后发生死亡的事件依然仍有发生.本研究分析了法国多个高流量胰腺外科中心2015年1月至2018年12月期间接受胰腺切除术90天内死亡的原因.共9个中心纳入研究患者3195例,术后90天内死亡140例,39例患者因故排除,有效分析101例.30%的死亡是术前评估或术后管理不善导致,约10%是不恰当的术中决策所致.高龄、联合动脉切除,尤其是计划外手术切除,是死亡相关危险因素.

关键词：胰腺手术胰腺外科胰腺切除术术前评估死亡原因分析计划外相关危险因素术后管理

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胰腺破骨巨细胞瘤

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世界核心医学期刊文摘（胃肠病学分册） 2005年第8期1卷 19-19页

作者： Beaufour A Cazals-Hatem D Regimbeau J.-M A. sauvanet 雷小英 Service de Chirurgie Digestive Hpital BeaujonAP-HP 100 bd du Gal Leclerc 92118 Clich y CedexFrance

Osteoclast giant cell tumours are bone tumours that occur in adults, and that are considered benign by WHO but locally aggressive. Strictly identical tumours are described in the pancreas,without simultaneous bone localization. We report the case of a 62-year woman with an osteoclast giant cell tumour of the distal pancreas, without any epithelial component, which was diagnosed after pancreatic resection and with no signs of recurrence after a 24-month follow-up. These p ancreatic tumours are rare, with a very poor prognosis, an unclear histogenesis; they are often confused with pleomorphic or undifferentiated pancreatic carcinom as including a component of osteoclast giant cell. These osteoclast giant cell t umours of the pancreas usually present as large cystic tumours. In certain cases , completeresection can result in long-term survival.

关键词：骨巨细胞瘤骨肿瘤侵蚀性骨组织上皮细胞组织发生

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联合肠系膜门静脉切除的胰十二指肠切除术治疗胰头腺癌

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中华肝胆外科杂志 2007年第5期13卷 360-360页

作者： Carrere N sauvanet a Goere D 李为民（摘）邹一平（校）不详解放军总医院第二附属医院北京市100091

胰腺癌侵犯门静脉被视为切除手术的禁忌证，一般认为门静脉系统孤立的肿瘤侵犯是根治性切除的最大障碍。而胰十二指肠切除术中联合肠系膜门静脉切除的价值一直存有争议。本文的主要目的是比较胰头癌病人接受单纯或联合胰十二指肠切除术... 详细信息

胰腺癌侵犯门静脉被视为切除手术的禁忌证，一般认为门静脉系统孤立的肿瘤侵犯是根治性切除的最大障碍。而胰十二指肠切除术中联合肠系膜门静脉切除的价值一直存有争议。本文的主要目的是比较胰头癌病人接受单纯或联合胰十二指肠切除术两组的死亡率、发病率、长期生存率以及术后预后参数。自1989年至2003年，共45例相连续的胰头癌病人接受胰十二指肠切除术，术中发现肠系膜上静脉或（和）门静脉被肿瘤侵犯而联合施行肠系膜门静脉切除（VR＋组）。同期88例胰头部腺癌病人接受了胰十二指肠切除而未联合肠系膜门静脉联合切除术（VR-组）。

关键词：胰十二指肠切除术肠系膜上静脉门静脉切除胰头腺癌治疗肿瘤侵犯侵犯门静脉根治性切除

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