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Extraction of DNA suitable for PCR applications from mature leaves of Mangifera indica L.

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第4期13卷 239-243页

作者： Muhammad Abubakkar AZMAT Iqrar Ahmad KHAN Hafiza Masooma Naseer CHEEMA Ishtiaq Ahmad RAJWANA Ahmad sattar KHAN Asif Ali KHAN Centre of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabadPakistan Department of Plant Breeding and Genetics University of Agricul-tureFaisalabadPakistan University College of Agriculture Bahauddin Zakariya UniversityMultanPakistan Institute of Horticultural Sciences University of AgricultureFais-alabadPakistan

Good quality deoxyribonucleic acid （DNA） is the pre-requisite for its downstream applications. The presence of high concentrations of polysaccharides, polyphenols, proteins, and other secondary metabolites in mango leaves poses problem in getting good quality DNA fit for polymerase chain reaction （PCR） applications. The problem is exacerbated when DNA is extracted from mature mango leaves. A reliable and modified protocol based on the cetyl trimethylammonium bromide （CTAB） method for DNA extraction from mature mango leaves is described here. High concentrations of inert salt were used to remove polysaccharides; Polyvinylpyrrolidone （PVP） and 13-mercaptoethanol were employed to man＇age phenolic compounds. Extended chloroform-isoamyl alcohol treatment followed by RNase treatment yielded 950-1050 pg of good quality DNA, free of protein and RNA. The problems of DNA degradation,contamination, and low yield due to irreversible binding of phenolic compounds and coprecipitation of polysaccharides with DNA were avoided by this method, The DNA isolated by the modified method showed good PCR amplification using simple sequence repeat （SSR） primers, This modified protocol can also be used to extract DNA from other woody plants having similar problems.

关键词： Extraction buffer Mango Polyphenols DNA isolation Simple sequence repeat (SSR) Secondary metabotites

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非酒精性脂肪肝

英国医学杂志中文版 2015年第1期18卷 35-41页

作者： Naveed sattar Ewan Forrest 王强 BHF Glasgow Cardiovascular Research CeriSe University of Glasgow Glasgow G12 8TA UK Department ofGastroenterology Glasgow Royal Infirmary GlasgowUK 湖北省武汉市黄陂区中医医院病理科

由于肥胖发生率的快速上升。目前相对于酒精性肝病来说，非酒精性脂肪肝（non—alcoholic fatty liver disease，NAFLD）更常见，且非酒精性脂肪肝是肝功能检查异常的最常见原因。其世界范围内的发生率据估计在一般人群约为20％、在2型... 详细信息

由于肥胖发生率的快速上升。目前相对于酒精性肝病来说，非酒精性脂肪肝（non—alcoholic fatty liver disease，NAFLD）更常见，且非酒精性脂肪肝是肝功能检查异常的最常见原因。其世界范围内的发生率据估计在一般人群约为20％、在2型糖尿病患者中高达70％。

关键词：非酒精性脂肪肝肥胖保健知识预防措施

Gender Politics in the Projection of ＂Disney＂ Villains

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Journal of Literature and Art Studies 2018年第1期8卷 53-57页

作者： Tania Sharmin Sanyat sattar Jahangimagar University Dhaka Bangladesh

Disney being one of the most influential media giants in the world, attracting both young and adults equally and creating memorable characters for almost 80 years, has introduced some memorable villain characters. But interestingly, the projection of male and female villains is rather different and often questionable. This paper aims to investigate how the evil characters have an unequal projection in terms of their gender roles.

关键词： villain gender animation masculinity gender-bending

Some Exact Solutions of Two Fifth Order KdV-Type Nonlinear Partial Differential Equations

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Journal of Partial Differential Equations 2012年第4期25卷 356-365页

作者： MIA Abdus sattar AKTER Tania Department of Mathematics Brock University St. Catharines Ontario Canada. Department of Mathematics World University of Bangladesh Dhaka Bangladesh.

We consider the generalized integrable fifth order nonlinear Korteweg-de Vries （fKdV） equation. The extended Tanh method has been used rigorously, by com- putational program MAPLE, for solving this fifth order nonlinear partial differential equation. The general solutions of the fKdV equation are formed considering an ansatz of the solution in terms of tanh. Then, in particular, some exact solutions are found for the two fifth order KdV-type equations given by the Caudrey-Dodd-Gibbon equation and the another fifth order equation. The obtained solutions include solitary wave solution for both the two equations.

关键词： 5th order KdV-type equation extended Tanh method CDG equation exact solutions solitary wave solution.

A Low Power and High Bandwidth Anti Aliasing Filter with Transconductance Structure for DAB Receivers

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通讯和计算机（中英文版） 2011年第5期8卷 373-380页

作者： Mohammad Mehdi Farhad sattar Mirzakuchaki Engineering-Department of Electronic Islamic Azad University Saveh branch Saveh 39197-15179 lran Electrical Engineering Department Electronic Research Center Iran University of Science ＆ Technology Tehran 16846 Iran

Exploring Metal Based Nanoparticles for Boosting Plant Tolerance to Heavy Metals and Trace Element Contamination

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Phyton-International Journal of Experimental Botany 2024年第11期93卷 2683-2705页

作者： Abdul Ghafoor Maria Latif Shafaqat Ali Muhammad Munir Muhammad Naeem sattar Mohammed Ali Alshehri Water and Environmental Study Centre King Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Environmental Sciences Government College University FaisalabadFaisalabad38000Pakistan Department of Biological Sciences and Technology China Medical UniversityTaichung40402Taiwan Date Palm Research Center of Excellence King Faisal UniversityAl-Ahsa31982Saudi Arabia Central Laboratories King Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Biology Faculty of ScienceUniversity of TabukTabuk71491Saudi Arabia

Heavy metal pollution in agricultural soils is a signiﬁcant challenge for global food production and human health with the increasing industrialization and *** is a concern about introducing innovative techni-ques that are eco-friendly,cost-effective,and have the potential to alleviate metals,enhance crop growth,and pro-tect plants against various environmental *** this,nanotechnology is one of the promising solutions having various applications in almost everyﬁeld of *** review explores various nano-based strategies that use nanoparticles(NPs)to lessen the harmful effects that heavy metals have on *** literature including published research and review papers from the year 2015 to *** review paper gives a thorough review of the current situation regarding heavy metal contamination in agricultural soils and how it affects plant *** necessity ofﬁnding practical and eco-friendly ways to address these issues is emphasized,paving the way for the introduction of ***,it highlighted the mechanistic route of heavy metal toxicity alleviation in plants by their application as well as their long-term efﬁciency and *** review also elaborated on var-ious synthesis methods(physical,chemical,and green),but the emphasis on the green synthesis of NPs by utiliz-ing plant extract offers dependable and sustainable beneﬁts over traditional physicochemical *** trace element stress,NPs application enhances plant antioxidant defense system,ameliorating structural changes,immobilizing trace elements in growth media,and improving the physio-chemical properties of soil as ***-ever,there are still numerous limitations present on how these materials are synthesized,applied,and appropri-ately absorbed by plant *** is recommended to promote and fund long-term research to assess the long-term effects of using NPs on plant development,soil health,and possible environmental repercussions.

关键词： Nanotechnology heavy metals toxicity agriculture physio-chemical attributes crop yield

A qualitative exploration of Pakistan’s street children, as a consequence of the poverty-disease cycle

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Infectious Diseases of Poverty 2014年第1期3卷 92-99页

作者： Muhammad Ahmed Abdullah Zeeshan Basharat Omairulhaq Lodhi Muhammad Hisham Khan Wazir Hameeda Tayyab Khan Nargis Yousaf sattar Adnan Zahid Department of Community and Family Medicine Shifa College of MedicinePitrus Bukhari RoadSector H-8/4IslamabadPakistan Department of Medicine Shifa International HospitalPitrus Bukhari RoadSector H-8/4IslamabadPakistan Department of Medicine District Headquarter Teaching HospitalRawalpindiPakistan

Background:Street children are a global phenomenon,with an estimated population of around 150 million across the *** children include those who work on the streets but retain their family contacts,and also those who practically live on the streets and have no or limited family *** Pakistan,many children are forced to work on the streets due to health-related events occurring at home which require children to play a financially productive role from an early *** explanatory framework adapted from the poverty-disease cycle has been used to elaborate these ***:This study is a qualitative study,and involved 19 in-depth interviews and two key informant interviews,conducted in Rawalpindi,Pakistan,from February to May *** data was audio taped and *** themes were identified and built *** respondents were contacted through a gatekeeper ex-street child who was a member of the street children ***:We asked the children to describe their life *** stories led us to the finding that street children are always forced to attain altered social roles because health-related problems,poverty,and large family sizes leave them no choice but to enter the workforce and earn their *** also gathered information regarding high-risk practices and increased risks of sexual and substance abuse,based on the street children’s increased *** children face the issue of social exclusion because diseases and poverty push them into a life full of risks and hazards;a life which also confines their social role in the ***:The street child community in Pakistan is on the *** children are excluded from mainstream society,and the absence of access to education and vocational skills reduces their future *** in mind the implications of health-related events on these children,robust inter-sectoral interventions are required.

关键词： Street children Poverty-disease cycle Pakistan

Development of Six-sigma Capability Indices in Modern Quality Management Practices

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Journal of Systems Science and Information 2006年第1期4卷 127-133页

作者： Abdul sattar Jamali Jinlin Li School of Management and Economics Beijing Institute of Technology Beijing 100081 China

This research work based on the development of 6σ capability indices in the Modern Quality Management Practices in the world level companies is briefly introduced. The process performance index （when a process indicated by control charts is under control） and the percentage of defective products are calculated and compared with kσ. Finally, compared 6σ with 3σ, when process is on nominal （nominal - mean）, the process has 1.5σ shift from nominal.

关键词： six-sigma capability indices parts per million

Antioxidant Activity of Pomegranate (<i>Punica granatum</i>L.) Fruit Peels

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Food and Nutrition Sciences 2012年第7期3卷 991-996页

作者： Mutahar S. Shiban mutlag M. Al-Otaibi Najeeb S. Al-zoreky Department of Food & Nutrition Sciences College of Agricultural and Food Sciences King Faisal University Al-Ahsa 31982 Saudi Arabia. Department of Food Science & Technology Faculty of Agriculture Sana’a University Sana’a Yemen

The antioxidant activity of pomegranate fruit peels was evaluated using in vitro tests. 80% methanolic extracts (ME) of peels had higher yield (45.4%) and total phenolics (27.4%) than water (WE) or ether extracts (EE). The reducing power of ME was more potent (P α-catechin. Pomegranate peels contained phenolics, exhibited DPPH scavenging activity and reducing power.

关键词： Punica Granatum Phenolic Compounds DPPH Scavenging Reducing Power