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Application of Green Fluorescent Protein in Immunoassays

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Advances in Bioscience and Biotechnology 2014年第6期5卷 557-563页

作者： Seiichi Sakamoto Yukihiro Shoyama Hiroyuki Tanaka satoshi morimoto Department of Pharmacognosy Faculty of Pharmaceutical Sciences Nagasaki International University Nagasaki Japan Department of Pharmacognosy Graduate School of Pharmaceutical Sciences Kyushu University Fukuoka Japan

Green fluorescent protein (GFP) is a protein that emits green fluorescence when exposed to a radiation of ultraviolet wavelength range, even without the addition of substrate and cofactor. Because of such characteristics, the usage of GFP is widespread in both in vivo and in vitro applications. In addition, recent advances in biotechnology have enabled GFP to be expressed in various hosts, including bacteria, yeast, plants, animals, and even living-cells, for multiple purposes. Currently, GFP is a subject of great interest in the analytical sciences, especially in immunoassays for qualitative and quantitative analyses, when it is fused with an antibody because of the high sensitivity of GFP and antigen-binding specificity of antibodies. Recently the fluobody, which is a fusion protein of GFP with single-chain variable fragment antibody (scFv), has become a useful tool in various fields. We review here the applications of GFP as fluobodies in immunoassays.

关键词： GFP Immunoassay Fluobody

Investigation of <i>β</i>-Cryptoxanthin Fatty Acid Ester Compositions in Citrus Fruits Cultivated in Japan

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Food and Nutrition Sciences 2013年第9期4卷 98-104页

作者： Yusuke Wada Atsuki Matsubara Takato Uchikata Yugo Iwasaki satoshi morimoto Katsuta Kan Tetsuya Ookura Eiichiro Fukusaki Takeshi Bamba Department of Biotechnology Graduate School of Engineering Osaka University Osaka Japan Food Function Division National Food Research Institute National Agricultural and Food Research Organization Tsukuba Japan. Food Research Center Ehime Institute of Industrial Technology Matsuyama Japan Graduate School of Bioagricultural Sciences Nagoya University Nagoya Japan Omishima Fruits Processing Co. Ltd. Imabari Japan

In this study, a catalog of β-cryptoxanthin (βCX) and its fatty acid esters (βCXFAs) in citrus fruits were constructed. The peel and pulp of citrus fruits from nine Japanese cultivars and one foreign cultivar were surveyed by supercritical fluid chromatography/triple quadrupole mass spectrometry (SFC/QqQMS). In each citrus sample, the major components were βCX and its laurate ester, myristate ester, palmitate ester, and oleate ester. However, the composition ratio of βCXFA varied with the citrus breed and between the pulp and the peel of the fruit in most cultivars. The SFC/QqQMS system could be used to obtain new information about βCXs and carry out further discussion on the feature and tendency of each citrus variety.

关键词： β-Cryptoxanthin Fatty Acid Ester Supercritical Fluid Chromatography

Electron scattering by Friedel oscillations in carbon nanotubes

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Nano Research 2022年第2期15卷 889-897页

作者： Takumi Inaba Takahiro morimoto satoshi Yamazaki Toshiya Okazaki CNT-Application Research Center National Institute of Advanced Industrial Science and TechnologyTsukuba 305-8565Japan Research Association of High-Throughput Design and Development for Advanced Functional Materials Tsukuba 305-8565Japan

Multi-walled carbon nanotube networks were confirmed to exhibit a linear decrease in resistivity with increasing temperature from 100 to above 400 *** linearity was explained using a defect scattering model that involved Friedel oscillations(that is,electron-electron interactions).The applicability of this model,which was originally proposed for graphene,to carbon nanotubes was assessed based on a comparison of various experimental *** in the slopes of the resistivity-temperature plots following the introduction of defects,as well as an effect of charge concentration on the slope were key predictions of this *** results obtained from few-walled carbon nanotube networks are also *** the literature,linear resistivity-temperature plots were obtained from other graphene derivatives,indicating that the linearity originates from the hexagonal symmetry of these *** present work also indicated a relationship between the appearance of linearity and negative magnetoresistance above 100 *** on a mechanism incorporating scattering in association with Friedel oscillations and conventional electron conduction models,the universality of resistivity-temperature plots obtained from carbon nanotube networks is introduced.

关键词： carbon nanotube electron transport electron-electron interaction Friedel oscillations magnetoresistance

Differentiation of focal liver lesions using three-dimensional ultrasonography: Retrospective and prospective studies

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World Journal of Gastroenterology 2010年第17期16卷 2109-2119页

作者： Kazushi Numata Manabu morimoto Akito Nozaki Michio Ueda Masaaki Kondo satoshi Morita Katsuaki Tanaka Gastroenterological Center Yokohama City University Medical Center4-57 Urafune-choMinami-kuYokohamaKanagawa 232-0024Japan Department of Biostatistics and Epidemiology Yokohama City University Medical Center4-57 Urafune-choMinami-kuYokohamaKanagawa 232-0024Japan

AIM: To differentiate focal liver lesions based on enhancement patterns using three-dimensional ultrasonography (3D US) with perflubutane-based contrast ***: Two hundred and eighty two patients with focal liver lesions,including 168 hepatocellular carcinomas (HCCs),63 metastases,40 hemangiomas and 11 focal nodular hyperplasias (FNHs),were examined by 3D US with perflubutane-based contrast *** ultrasound images and sonographic angiograms were *** 282 lesions,enhancement patterns of 163 lesions between January 2007 and October 2007 were analyzed *** from November 2007 to May 2008,compared with contrast-enhanced (CE) 2D US,CE 3D US was performed on 119 lesions for prospective differential ***,specificity,area under receiver operating characteristic curve (Az) and inter-reader agreement were ***: With the tridimensional view,dominant enhancement patterns were revealed as diffuse enhancement or peripheral ring-like enhancement,followed with washout change for HCCs or metastases,respectively,and peripheral nodular enhancement or diffuse enhancement with spoke-wheel arteries,followed by persistent enhancement for hemangiomas or FNHs,*** CE 3D US,the prospective differentiation of lesions showed sensitivity 92% (mean for two readers),specificity 91% and Az value 0.95 for HCCs,84%,97%,and 0.95 for metastases,91%,98%,and 0.98 for hemangiomas and 80%,99%,and 0.99 for FNHs,respectively,while good to excellent inter-reader agreement was *** significant difference exists between prospective diagnosis accuracy at CE 3D US and that at CE 2D ***: CE 3D US provides a spatial perspective for liver tumor enhancement,and could help in differentiating focal liver lesions.

关键词： Three-dimensional Ultrasonography Contrast agent Liver Neoplasms

Long-term survival after resection of pancreatic cancer:A single-center retrospective analysis

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World Journal of Gastroenterology 2015年第1期21卷 262-268页

作者： Takehito Yamamoto Shintaro Yagi Hiromitsu Kinoshita Yusuke Sakamoto Kazuyuki Okada Kenji Uryuhara Takeshi morimoto satoshi Kaihara Ryo Hosotani Department of Surgery Kobe City Medical Center General HospitalHyogo 650-0047Japan Clinical Research Center Kobe City Medical Center General HospitalHyogo 650-0047JapanChief Statistician

AIM: To retrospectively analyze factors affecting the long-term survival of patients with pancreatic cancer who underwent pancreatic ***: From January 2000 to December 2011,195 patients underwent pancreatic resection in our *** prognostic factors after pancreatic resection were analyzed in all 195 *** excluding the censored cases within an observational period,the clinicopathological characteristics of 20 patients who survived ≥ 5(n = 20) and < 5(n = 76) years were *** this comparison,we analyzed the patients who underwent surgery before June 2008 and were observed for more than 5 *** statistical analyses,the log-rank test was used to compare the cumulative survival rates,and the χ2 and Mann-Whitney tests were used to compare the two *** CoxHazard model was used for a multivariate analysis,and P values less than 0.05 were considered significant.A multivariate analysis was conducted on the factors that were significant in the univariate ***: The median survival for all patients was 27.1 months,and the 5-year actuarial survival rate was 34.5%.The median observational period was 595 *** the univariate analysis,the UICC stage was significantly associated with survival time,and the CA19-9 ≤ 200 U/m L,DUPAN-2 ≤ 180 U/m L,t u m o r s i ze ≤ 2 0 m m,R 0 re s e c t i o n,a b s e n c e o f lymph node metastasis,absence of extrapancreatic neural invasion,and absence of portal invasion were favorable prognostic *** multivariate analysis showed that tumor size ≤ 20 mm(HR = 0.40; 95%CI: 0.17-0.83,P = 0.012) and negative surgical margins(R0 resection)(HR = 0.48; 95%CI: 0.30-0.77,P = 0.003) were independent favorable prognostic *** the 96 patients,20 patients survived for 5 years or more,and 76 patients died within 5 years after *** of the 20 5-year survivors with the 76 non-survivors showed that lower concentrations of DUPAN-2(79.5 vs 312.5 U/mL,P = 0.032),tumor size ≤ 20 mm(

关键词： Pancreatic cancer Long-term survival Longterm surv

Advances in quantum dots for classical and non-classical light sources Invited Paper

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Chinese Optics Letters 2008年第10期6卷 718-723页

作者： Yasuhiko Arakawa satoshi Iwamoto satoshi Kako Masahiro Nomura Denis Guimard Research Center for Advanced Science and Technology Institute for Nano Quantum Information ElectronicsThe University of Tokyo4-6-1 Meguro-ku153-8505 TokyoJapan

Recent advances in quantum dots （QDs） for classical and non-classical light sources are presented. We have established metal organic chemical vapor deposition （MOCVD） technology for InAs-based QD lasers at 1.3 μm and achieved ultralow threshold in QD lasers with photonic crystal （PhC） nanocavity. In addition, single photon emitters at 1.55 μm, GaN-based single photon sources operating at 200 K, and high-Q PhC nanocavity have been demonstrated.

关键词： QDs Advances in quantum dots for classical and non-classical light sources Invited Paper AIN InAs

Application of 3D Scanned Big Data of Large-scale Cultural Heritage Objects Based on Noise-robust Transparent Visualization

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系统仿真学报 2023年第8期35卷 1635-1650页

作者： Tanaka satoshi College of Information Science and Engineering Ritsumeikan UniversityShiga 525-8577Japan

Three-dimensional(3D) scanning technology has undergone remarkable developments in recent *** acquired by 3D scanning have the form of 3D point *** 3D scanned point clouds have data sizes that can be considered big *** also contain measurement noise inherent in measurement *** properties of 3D scanned point clouds make many traditional CG/visualization techniques *** paper reviewed our recent achievements in developing varieties of high-quality visualizations suitable for the visual analysis of 3D scanned point *** demonstrated the effectiveness of the method by applying the visualizations to various cultural heritage *** main visualization targets used in this paper are the floats in the Gion Festival in Kyoto(the float parade is on the UNESCO Intangible Cultural Heritage List) and Borobudur Temple in Indonesia(a UNESCO World Heritage Site).

关键词： 3D scanning point cloud transparent visualization noise transparentization cultural heritage object

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Posture Maintenance Control of 2-Link Object By Nonprehensile Two-Cooperative-Arm Robot Without Compensating Friction

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IEEE/CAA Journal of Automatica Sinica 2019年第6期6卷 1397-1403页

作者： Changan Jiang satoshi Ueno Department of Mechanical Engineering Ritsumeikan University1-1-1 Noji-higashiKusatsuShiga 525-8577Japan

In this paper, a method to posture maintenance control of 2-link object by nonprehensile two-cooperative-arm robot without compensating friction is proposed. In details, a mathematical model of the 2-link object is firstly built. Based on the model, stable regions for holding motion of nonprehensile two-cooperative-arm robot are obtained while the 2-link object is kept stable on the robot arms with static friction. Among the obtained stable regions, the robust pairs of orientation angles of the 2-link object are found. Under the robust orientation angles, a feedback control system is designed to control the arms to maintain the 2-link object's posture while it is being held or lifted up. Finally, experimental results are shown to verify the effectiveness of the proposed method.

关键词： Nursing care nonprehensile robot posture maintenance static friction two-cooperative-arm robot

A pH-dependent molecular switch for virion uncoating

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Protein & Cell 2014年第9期5卷 653-654页

作者： satoshi Koike Neurovirology Project Tokyo Metropolitan Institute of Medical Science 2-1-6 Kamikitazawa Setagaya-ku Tokyo 156-8506Japan

Enterovirus 71, belonging to human enterovirus speCies A in the genus Enterovirus of the family Picornaviridae, is one of the main causative agents of hand-foot-mouth disease. It can also cause severe neurological diseases such as acute encephalitis, polio-like paralysis, and neurogenic pulmonary edema, and it has become a serious public health concern especially in the Asia-Pacific region.

关键词： Enterovirus Picornaviridae Asia-Pacific