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Construction,expression and binding specificity of bispecific CD3×VeGFR-2 and CD3×NCAM antibodies in the single chain and diabody format

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Advances in Bioscience and Biotechnology 2013年第5期4卷 654-664页

作者： Anke Kopacek Thomas Boldicke sarah Lergenmüller Frank Berthold Markus Jensen Peter P.Müller Ludger Grosse-Hovest Helmholtz Center for Infection Research Department of Molecular BiotechnologyBraunschweigGermany Department of Pediatric Oncology and Hematology University of CologneUniversity Hospital CologneCologneGermany Institute for Cell Biology Department of ImmunologyEberhard Karls UniversityTübingenGermany

Bispecific antibodies are recombinant proteins with novel immunological properties and therapeutic potential. Recombinant protein quality and activity of several bispecific antibodies comprising different variable domain combinations with respect to the parental monospecific single chain fragments (scFv) were evaluated after expression in bacteria or mammalian cells. The parental scFv proteins humanized anti-NCAM scFv, murine anti-VeGFR-2 scFv, murine and humanized anti-CD3 scFv, respectively, could successfully be expressed in e. coli, whereas the murine anti-NCAM scFv version could not be reliably detected. Bispecific CD3 × VeGFR-2 and CD3 × NCAM anti-bodies were expressed in the bispecific single chain and the single chain diabody format. However, the diabody derived from the murine anti-NCAM scFv could not efficiently be expressed in e. coli or in mammalian cells. Significant binding of the CD3 × NCAM single chain diabody comprising the humanized version of anti-CD3 and humanized version of anti-NCAM was efficient to both antigens. Nevertheless, binding of the bispecific single chain version to the NCAM antigen was inefficient in comparison to CD3 binding. In conclusion, the data could indicate that the result of scFv expression in bacteria may be predictive for the chances of success for functional expression of more complex bispecific derivatives.

关键词： Recombinant Antibody Single Chain Diabody Bispecific Antibody Protein expression

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好吧,但这真是文学吗?

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新东方英语（中英文版） 2012年第12期 53-55页

作者： C.Alan Joyce sarah Janssen 思雅达翻译社

也许你听说过乔伊斯的《尤利西斯》（Ulysses）很难读，但是你知道他的另一部作品里长达一百字字母的单词吗？字母e是英语中出现频率最高的字母，

关键词：文学《尤利西斯》出现频率乔伊斯字母

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灵契

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2017年

作者： (英)萨拉·沃特斯(sarah Waters)

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历史视角下的“地方转向”:来自英国和德国的两个城市案例研究

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国际行政科学评论（中文版） 2017年第4期 125-144页

作者：萨拉·e.哈克特崔玲(译) 陈叶盛(审校) 孙春晖(审校) 巴思·斯帕大学不详

本文从历史视角探讨英国泰恩河畔纽卡斯尔市和德国不莱梅市在移民与融合政策上的“地方转向”。本文广泛使用政府文献资料并对这两个城市自20世纪60年代以来的政策进行比较评估。本文讨论的是政策制定的纵向维度,尽管探索的是国家层面... 详细信息

本文从历史视角探讨英国泰恩河畔纽卡斯尔市和德国不莱梅市在移民与融合政策上的“地方转向”。本文广泛使用政府文献资料并对这两个城市自20世纪60年代以来的政策进行比较评估。本文讨论的是政策制定的纵向维度,尽管探索的是国家层面移民融合的地方治理。虽然人们通常认为英国和德国的战后移民历史和政治结构有着显著差异,但是本文却揭示出这两个城市的政府在管理各自的当地多元社会时趋于一致。这些案例研究质疑国家宏观宪法结构对于城市移民政策的长期影响。本文的研究结果要在地方治理与多层治理的讨论框架下理解。

关键词：不莱梅市市级的移民与融合政策历史多层治理泰恩河畔纽卡斯尔市政治趋同

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The Skin Microbiome of <i>Gambusia affinis</i>Is Defined and Selective

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Advances in Microbiology 2014年第7期4卷 335-343页

作者： Annie B. Leonard Jeanette M. Carlson Dayna e. Bishoff sarah I. Sendelbach Sonja B. Yung Sonya Ramzanali Ananda B. W. Manage embriette R. Hyde Joseph F. Petrosino Todd P. Primm Alkek Center for Metagenomics and Microbiome Research Department of Molecular Virology and Microbiology Baylor College of Medicine Houston USA Department of Biological Sciences Sam Houston State University Huntsville USA Department of Mathematics and Statistics Sam Houston State University Huntsville USA

Metagenomics and bacterial culture were used to determine the normal skin microbiome of the Western mosquitofish (Gambusia affinis). This is the first study of G. affinis, and the most in-depth study of any fish skin, utilizing a combination of 16S profile pyrosequencing and culture analysis. Over 1800 sequences obtained from three individuals reveal that over half of all sequences come from five invariant genera, Acinetobacter, Sphingomonas, Acidovorax, enhydrobacter, and Aquabacterium. The microbiome is diverse but has low equitability, with a total of 81 genera detected. Challenge studies suggest that non-native bacteria cannot colonize the skin. This definition of the normal skin microbiome lays the foundation for future studies with this model system.

关键词： Fish Microbiome Acinetobacter Sphingomonas Metagenomics Mucosal

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雀巢在革自己的命

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商业周刊（中文版） 2016年第10期 68-73页

作者： Matthew Campbell Corinne Gretler sarah Anne Ward 杨飞

雀巢（Nesu e）是目前全世界最大的食品公司。该公司在全球85个国家和地区有436家工厂、33．5万名员工，旗下品牌2000个。它是欧洲最值钱的公司，价值2400亿美元，将石油巨头荷兰皇家壳牌石油公司（Royal Dutch Shell）远远甩在后面。

关键词：壳牌石油公司食品公司 Dutch 品牌美元

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Microgeographic Variation and Inter-Riffle Migration of <i>Rhinichthys atratulus</i>(Pisces: Cyprinidae) in a Small Connecticut Stream, United States

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Open Journal of ecology 2020年第7期10卷 460-481页

作者： Sage J. Loomis Kayla Anatone Laura Bither sarah J. Kang Nola Neri David Machado Michelle L. Kraczkowski Barry Chernoff College of the environment Wesleyan University Middletown USA Department of Biology Wesleyan University Middletown USA Department of Molecular Biology and Biochemistry Wesleyan University Middletown USA Department of Biology University of Saint Joseph West Hartford USA Department of earth & environmental Sciences Wesleyan University Middletown USA

We examined the genetic diversity on a microgeographic scale of Rhinichthys atratulus (eastern Blacknose Dace) in Allyn Brook, a small tributary in the upper Coginchaug River drainage in Connecticut. By looking at gen... 详细信息

We examined the genetic diversity on a microgeographic scale of em>Rhinichthys atratulusem> (eastern Blacknose Dace) in Allyn Brook, a small tributary in the upper Coginchaug River drainage in Connecticut. By looking at gene flow on a microgeographic scale among populations that had no physical barriers to migration, we tested the null hypothesis that the populations should be homogeneous. We resolved seven polymorphic microsatellite loci and one mitochondrial gene, em>ndem>2, in three adjacent populations (ed these populations to the two closest populations (>5 km) in the Coginchaug River. A dam from the 1920’s in lower Allyn Brook has isolated Allyn-Brook populations from Coginchaug-River populations. Allyn Brook was selected because there are only three riffle habitats in the brook and, therefore, there can be no immigration from upstream populations. each population has private (em>i.eem>., unique) alleles and haplotypes, and there are significant genetic differences between all sites. The Allyn Brook populations are almost as different from one another as they are from the distant populations in the Coginchaug River from which they have been isolated for more than 80 years. These results point to em>in situem> evolution and little migration or gene flow among populations on a microgeographic scale. This raises interesting questions for conservation of genetic diversity of stream fishes.

关键词： Migration Microgeographic Differentiation Population ecology Gene Flow Rhinichthys atratulus

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东、西方蜜蜂混合饲养蜂群的温度调节

东、西方蜜蜂混合饲养蜂群的温度调节

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中国养蜂学会中蜂协作委员会“2009年学术交流会”

作者：杨明显汪正威李华张祖芸谭垦 sarah e. Radloff H. Randall Hepburn Department of Zoology and entomology Rhodes University 云南农业大学东方蜜蜂研究所 Department of Statistics Rhodes University

东、西方蜜蜂有着不同的降低巢温的策略。本文的目的是研究在混合饲养蜂群内两种蜜蜂的行为表现,看它们能否使群内温度达到稳定。结果显示在混合群育虫区温度更接近纯东方蜜蜂蜂群的温度。在加温条件下,东方蜜蜂工蜂更敏感而比西方蜜蜂... 详细信息

东、西方蜜蜂有着不同的降低巢温的策略。本文的目的是研究在混合饲养蜂群内两种蜜蜂的行为表现,看它们能否使群内温度达到稳定。结果显示在混合群育虫区温度更接近纯东方蜜蜂蜂群的温度。在加温条件下,东方蜜蜂工蜂更敏感而比西方蜜蜂先开始扇风降温。在混合群里,东方蜜蜂是温度调节的主要力量。当两种工蜂都在巢门口扇风,它们都有各自独特的姿态,但由于只有一小部分的西方蜜蜂工蜂参与扇风,混合群的降温效率更接近东方蜜蜂群。

关键词：温度调节混合群 Apis cerana Apis mellifera

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Risk factors for ribavirin treatment failure in Asian organ transplant recipients with chronic hepatitis e infection

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World Journal of Hepatology 2019年第6期11卷 553-561页

作者： en Xian sarah Low edhel Tripon Kieron Lim Poh Seng Tan How Cheng Low Yock Young Dan Yin Mei Lee Mark Muthiah Wai Mun Loo Calvin Jianyi Koh Wah Wah Phyo JunXiong Pang Seng Gee Lim Guan-Huei Lee Department of Medicine Ng Teng Fong General HospitalNational University Health SystemSingapore 609606Singapore Centre for Liver Disease Management and Transplant of Medical City Manila 1605Philippines Mount elizabeth Medical Centre Singapore 228510Singapore Division of Gastroenterology and Hepatology National University Health SystemSingapore 119228Singapore Department of Medicine National University of SingaporeSingapore 119077Singapore Centre for Infectious Disease epidemiology and Research National University of SingaporeSingapore 117549Singapore Department of Medicine Yong Loo Lin School of MedicineNational University of SingaporeSingapore 119228Singapore

BACKGROUND Hepatitis e virus(HeV)infection is a cause of chronic hepatitis in immunosuppressed *** virologic response rates to a 12-wk course of ribavirin therapy were reported to be>70%in the *** study describes the outcome of HeV treatment in a transplant center in *** To study the outcome of ribavirin treatment in a series of chronic HeV patients,and the cause of treatment *** We studied all of the transplant recipients who were diagnosed with HeV infection between 2012 to *** outcome of therapy and virologic relapse are monitored for three years after the end of *** Ten transplant recipients(4 liver,5 kidney,and 1 bone marrow transplantation)with positive HeV RNA were *** patients received at least 12 wk of ribavirin therapy,and the remaining patient resolved after reducing immunosuppression *** subjects had prolonged viremia that lasted more than one year,despite continuous ribavirin *** ribavirin-treated patients(44.4%)had HeV RNA relapse after achieving a virologic response by the end of *** overall failure rate is 66.7%.Being a kidney transplant recipient is the strongest risk factor for not achieving an initial sustained virologic response(0/5 treated,Chi-Square test,Pe most common side effect of ribavirin is anemia(100%)(haemoglobin reduction of 3-6.2 g/dL).Seven patients required either a blood transfusion or erythropoietin *** The sustained virologic response rate of 12-wk ribavirin therapy for HeV infection in this Asian series was lower than *** transplant recipients had a higher rate of treatment failure due to higher immunosuppression requirements and adverse effects.

关键词： Toxicity Antiviral agents Hepatitis e virus Virus classification Systemic immunity Immune responses Persistent infection

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《L’élégance du hérisson》

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明日风尚 2009年第5期 195-195页

作者： sarah Khider

作者:Muriel Barbery出版:GAL LMARD"我刚读完一本非常美的法语小说《L’élégance du hérisson》(中译:《刺猬的优雅》),作者的写作风格很优美,文章充满了人性、智慧和敏锐,我希望所有人都可以读读它。故事围绕着两位‘刺猬女性’的... 详细信息

作者:Muriel Barbery出版:GAL LMARD"我刚读完一本非常美的法语小说《L’élégance du hérisson》(中译:《刺猬的优雅》),作者的写作风格很优美,文章充满了人性、智慧和敏锐,我希望所有人都可以读读它。故事围绕着两位‘刺猬女性’的故事展开。一位名叫Renée,是位巴黎豪宅的看门老太太,终日看些无聊的泡沫剧,外人看来她就是一个没有文化的穷寡妇。但事实与表面相差甚远,她通晓哲学、古典音乐和抽象画,可她相信地位卑微的人永远会被拒于艺术殿堂的门外,所以宁愿掩藏自己。而另一位‘刺猬’是住在这栋房子里的12岁富家千金Paloma,她是个天才少女却不得不整天在大人面前装笨,生活得很不开心,她从周围成年人身上看透了生命的平庸和荒谬,害怕自己也会重蹈覆辙,甚至想过自杀了结生命。这两个主人公之间发展的故事非常意味深长,读了你就会爱上它。"

关键词：写作风格 gance du h risson 一本艺术殿堂

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