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Insulin-like growth factor binding protein-5 influences pancreatic cancer cell growth

World Journal of Gastroenterology 2009年第27期15卷 3355-3366页

作者： sarah k johnson Randy S Haun Department of Pathology Winthrop P. Rockefeller Cancer Institute University of Arkansas for Medical SciencesLittle Rock Arkansas AR 72205 United States

AIM： To investigate the functional significance of insulin-like growth factor binding protein-5 （IGFBP-5） overexpression in pancreatic cancer （PaC）.METHODS： The effects of IGFBP-5 on cell growth were assessed by stable transfection of BxPC-3 and PANC-1 cell lines and measuring cell number and DNA synthesis. Alterations in the cell cycle were assessed by flow cytometry and immunoblot analyses. Changes in cell survival and signal transduction were evaluated after mitogen and phosphatidylinositol activated protein kinase 3-kinase （PI3k） inhibitor treatment.RESULTS： After serum deprivation, IGFBP-5 expression increased both cell number and DNA synthesis in BxPC-3 cells, but reduced cell number in PANC-1 cells. Consistent with this observation, cell cycle analysis of IGFBP-5-expressing cells revealed accelerated cell cycle progression in BxPC-3 and G2/M arrest of PANC-1 cells. Signal transduction analysis revealed that Akt activation was increased in BxPC-3, but reduced in PANC-1 cells that express IGFBP-5. Inhibition of PI3k with LY294002 suppressed extracellular signal-regulated kinase-1 and -2 （ERk1/2） activation in BxPC-3, but enhanced ERk1/2 activation in PANC-1 cells that express IGFBP-5. When MEk1/2 was blocked, Akt activation remained elevated in IGFBP-5 expressing PaC cells; however, inhibition of PI3k or MEk1/2 abrogated IGFBP-5-mediated cell survival.CONCLUSION： These results indicate that IGFBP-5 expression affects the cell cycle and survival signal pathways and thus it may be an important mediator of PaC cell growth.

关键词： Insulin-like growth factor-binding protein 5 Extracellular signal-regulated mitogen activated protein kinases Cyclin-dependent kinase inhibitor p27 Pancreatic neoplasms

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How University Athletics Can Impact Mental Health among Student Athletes at the University of Evansville

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Health 2024年第3期16卷 218-233页

作者： Claudia Hollis Blake johnson Salma Gonzalez sarah Harness College of Education and Health Sciences University of Evansville Evansville USA

In recent years, the NCAA student athlete population in the United States has surpassed 500,000, and is continuing to rise each year [1]. These student athletes work their entire lives academically and athletically to reach the ultimate goal: competing in university athletics. However, when these athletes reach university, they are met with non-stop training, homework, exams, and evolving social lives. We have conducted a study at the University of Evansville evaluating how participation in university athletics may impact mental health status among these student athletes, as well as measuring players’ awareness of accessible mental health resources. Over fifty percent of participants reported experiencing at least one mental health condition while competing in their sport;eighty percent reported having knowledge of the mental health resources available to them on campus, however, nearly thirty percent of those knowledgeable reported not knowing how to access these resources. This has indicated a gap in awareness and utilization of mental health resources among student athletes at the University of Evansville.

关键词： Student Athlete Mental Health Mental Health Resources

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Influence of Soil Properties on Zinc Solubility Dynamics Under Different Redox Conditions in Non-Calcareous Soils

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Pedosphere 2017年第1期27卷 96-105页

作者： Michelle Anne Belen BUNQUIN Susan TANDY sarah johnson BEEBOUT Rainer SCHULIN Crop and Environmental Sciences Division International Rice Research InstituteCollegeLos Banos Soil Protection Institute of Terrestrial EcosystemsETH Zurich

Zinc(Zn) deficiency in paddy soils is often a problem for rice *** can decrease metal availability in some noncalcareous soils through different mechanisms associated with soil redox *** experiments were performed in order to better understand the processes that governed the dynamics of Zn in non-calcareous paddy soils at varying redox potentials(Eh).Airdried non-calcareous soil samples collected from four different paddy field sites in the Philippines were submerged and incubated in a reaction cell with continuous stirring and nitrogen purging for 4 weeks,and then purged with compressed air for another week to reoxidize the *** Eh of the four soils started at 120 to 300 mV,decreased to —220 to —300 mV after 100 to 250 h of reduction,and was maintained at this low plateau for about 2 weeks before increasing again upon *** solubility showed contrasting patterns in the four soils,with two of the soils showing a decrease in soluble Zn as the Eh became low,probably due to zinc sulfide(ZnS) *** contrast,the other two soils showed that Zn solubility was maintained during the reduced phase which could be due to the competition with iron(Fe) for precipitation with *** in the relative amounts of S,Fe,and manganese(Mn) oxides in the four soils apparently influenced the pattern of Zn solubility after flooding.

关键词： microcosm paddy soil precipitation rice soil sulfide speciation Zn availability

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Multiple Access Technologies for Cellular M2M Communications

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ZTE Communications 2016年第4期14卷 42-49页

作者： Mahyar Shirvanimoghaddam sarah J.johnson School of Electrical Engineering and Computer Science The University of Newcastle

This paper reviews the multiple access techniques for machine-to-machine(M2M) communications in future wireless cellular networks. M2M communications aims at providing the communication infrastructure for the emerging Internet of Things(IoT), which will revolutionize the way we interact with our surrounding physical environment. We provide an overview of the multiple access strategies and explain their limitations when used for M2M communications. We show the throughput efficiency of different multiple access techniques when used in coordinated and uncoordinated scenarios. Non-orthogonal multiple access(NOMA) is also shown to support a larger number of devices compared to orthogonal multiple access techniques, especially in uncoordinated scenarios. We also detail the issues and challenges of different multiple access techniques to be used for M2M applications in cellular networks.

关键词： Internet of Things(IoT) massive access machine-to-machine(M2M) communications multiple access

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淹水土壤有机酸积累与秸秆碳氮比及氮供应的关系

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土壤学报 2006年第6期43卷 941-947页

作者：单玉华蔡祖聪韩勇 sarah E johnson Roland J Buresh 扬州大学环境科学与工程学院江苏扬州225009 中国科学院南京土壤研究所南京210008 International Rice Research Institute

有机酸积累和毒害是稻田秸秆还田中受到广泛关注的问题。本文以水稻与小麦秸秆为材料,采用淹水培养研究了甲酸、乙酸、丙酸及丁酸在土壤中的积累及其与秸秆碳氮比、氮肥添加量的关系。结果表明,在不施用氮肥的情况下,随秸秆用量的增加,... 详细信息

有机酸积累和毒害是稻田秸秆还田中受到广泛关注的问题。本文以水稻与小麦秸秆为材料,采用淹水培养研究了甲酸、乙酸、丙酸及丁酸在土壤中的积累及其与秸秆碳氮比、氮肥添加量的关系。结果表明,在不施用氮肥的情况下,随秸秆用量的增加,秸秆处理的有机酸积累均显著增多。与稻秸处理相比,麦秸处理的有机酸(尤其是丙酸)积累量显著较高,土壤溶液中NH4+浓度显著较低。加入尿素明显减少有机酸积累,促进CH4排放,但对CO2的排放无显著影响;氮素的影响在麦秸处理中表现的尤为明显。上述结果说明麦秸的高碳氮比增加了无机氮的生物固定,抑制有机酸向CH4转化,从而导致麦秸处理有机酸积累量高于稻秸处理。施用氮肥是减少麦秸还田后有机酸积累的有效措施之一,但此措施将可能促进CH4的排放。

关键词：淹水土壤有机酸秸秆碳氮比气体排放氮肥

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International validation of the Chinese University Prognostic Index for staging of hepatocellular carcinoma: a joint United kingdom and Hong kong study

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Chinese Journal of Cancer 2014年第10期33卷 481-491页

作者： Stephen L.Chan Philip J.johnson Frankie Mo sarah Berhane Mabel Teng Anthony W.H.Chan Ming C.Poon Paul B.S.Lai Simon Yu Anthony T.C.Chan Winnie Yeo State key Laboratory in Oncology in South China Sir YK Pao Center for CancerDepartment of Clinical OncologyHong Kong Cancer Institute and Prince of Wales HospitalThe Chinese University of Hong Kong School of Cancer Sciences University of Birmingham the Department of Molecular and Clinical Cancer Medicine Institute of Translational MedicineUniversity of Liverpool Department of Anatomical Cellular and PathologyThe Chinese University of Hong Kong Department of Surgery The Chinese University of Hong Kong Department of Radiology and Organ Imaging The Chinese University of Hong Kong

The outcome of hepatocellular carcinoma(HCC) patients significantly differs between western and eastern population centers. Our group previously developed and validated the Chinese University Prognostic Index(CUPI) for the prognostication of HCC among the Asian HCC patient population. In the current study, we aimed to validate the CUPI using an international cohort of patients with HCC and to compare the CUPI to two widely used staging systems, the Barcelona Clinic Liver Cancer(BCLC) classification and the Cancer of the Liver Italian Program(CLIP). To accomplish this goal, two cohorts of patients were enrolled in the United kingdom(Uk; n = 567; 2006-2011) and Hong kong(Hk; n = 517; 2007-2012). The baseline clinical data were recorded. The performances of the CUPI, BCLC, and CLIP were compared in terms of a concordance index(C-index) and were evaluated in subgroups of patients according to treatment intent. The results revealed that the median follow-up durations of the Uk and Hk cohorts were 27.9 and 29.8 months, respectively. The median overall survival of the Uk and Hk cohorts were 22.9 and 8.6 months, respectively. The CUPI stratified the patients in both cohorts into three risk subgroups corresponding to distinct outcomes. The median overall survival of the CUPI low-, intermediate-, and high-risk subgroups were 3.15, 1.24, and 0.29 years, respectively, in the Uk cohort and were 2.07, 0.32, and 0.10 years, respectively, in the Hk cohort. For the patients who underwent curative treatment, the prognostic performance did not differ between the three staging systems, and all were suboptimal. For those who underwent palliative treatment, the CUPI displayed the highest C-index, indicating that this staging system was the most informative for both cohorts. In conclusion, the CUPI is applicable to both western and eastern HCC patient populations. The performances of the three staging systems differed according to treatment intent, and the CUPI was demonstrated to be opti

关键词：香港英国预后肝癌验证国际大学中国

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Characterization of a novel murine Sost ERT2 Cre model targeting osteocytes

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Bone Research 2019年第1期7卷 41-53页

作者： Delphine B.Maurel Tsutomu Matsumoto Julian A.Vallejo Mark L.johnson sarah L.Dallas Yukiko kitase Marco Brotto Michael J.Wacker Marie A.Harris Stephen E.Harris Lynda F.Bonewald Department of Oral and Craniofacial Sciences University of Missouri-Kansas City School of DentistryKansas CityMOUSA Department of Anatomy and Cell Biology Indiana University School of MedicineIndianapolisINUSA Bone.Muscle Collaborative Sciences College of Nursing and Health InnovationUniversity of Texas at ArlingtonArlingtonUSA Department of Biomedical Sciences University of Missouri-Kansas City School of MedicineKansas CityMOUSA University of Texas Health Science Center San AntonioTXUSA Department of Orthopaedic Surgery Indiana University School of MedicineIndianapolisINUSA Pharmaceutical Sciences Department Universite de BordeauxBio-TisINSERM Unité1026 BioTis146 Rue Léo Saignat33076 BordeauxFrance

Transgenic mice are widely used to delete or overexpress genes in a cell specific manner to advance knowledge of bone biology,function and *** numerous Cre models exist to target gene recombination in osteoblasts and osteoclasts,few target osteocytes specifically,particularly mature *** goal was to create a spatial and temporal conditional Cre model using tamoxifen to induce Cre activity in mature osteocytes using a Bac construct containing the 5’and 3’regions of the Sost gene(Sost ERT2 Cre).Four founder lines were crossed with the Ai9 Cre reporter *** founder line showed high and specific activity in mature *** and organs were imaged and fluorescent signal *** no activity was observed in 2 day old pups,by 2 months of age some osteocytes were positive as osteocyte Cre activity became spontaneous or‘leaky’with *** percentage of positive osteocytes increased following tamoxifen injection,especially in males,with 43%to 95%positive cells compared to 19%to 32%in *** signal was observed in any bone surface cell,bone marrow,nor in muscle with or without tamoxifen *** spontaneous signal was observed in any other ***,with tamoxifen injection,a few positive cells were observed in kidney,eye,lung,heart and *** other organs,28 in total,were negative with tamoxifen ***,with age,a muscle phenotype was apparent in the Sost-ERT2 Cre ***,although this mouse model may be useful for targeting gene deletion or expression to mature osteocytes,the muscle phenotype may restrict the use of this model to specific applications and should be considered when interpreting data.

关键词： cell knowledge model

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Diagnosis and Treatment of Tolosa-Hunt Syndrome in the Emergency Department

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Open Journal of Emergency Medicine 2017年第1期5卷 1-7页

作者： kevin Olsen Andrew johnson sarah Castillo-Jorge Jeffrey Bennett Matthew F. Ryan Department of Anaesthesiology University of Florida Gainesville FL USA Department of Emergency Medicine University of Florida Gainesville FL USA Department of Radiology University of Florida Gainesville FL USA

We describe herein a patient who presented with painful ophthalmoplegia and was ultimately diagnosed via magnetic imaging resonance studies and successfully treated for Tolosa-Hunt syndrome. Tolosa-Hunt syndrome is a rare, reversible and painful ophthalmoplegia characterized by recurrent unilateral orbital pain, ipsilateral oculomotor paralysis and prompt response to steroids. Specific criteria for its diagnosis exist and are discussed herein. Individuals affected may display signs of select cranial nerve palsies, ptosis, facial numbness, diplopia, midrosis, and proptosis. Appropriate recognition of the disease can allow for immediate intervention and thus decrease the length and severity of symptoms especially as symptoms may not spontaneously resolve without treatment which leads to unnecessary suffering through pain, anxiety, and decreased vision. We describe the case presentation and keys for diagnosis emergency medicine that physicians should know for this potentially devastating condition.

关键词： Tolosa-Hunt Syndrome Ophthalmoplegia Vision Loss MRI MRA Cranial Nerve Deficits

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The keele curriculum model: A contemporary framework for designing an inter-professional technology enhanced nursing curriculum

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Open Journal of Nursing 2013年第4期3卷 358-362页

作者： Melanie Humphreys Ian Wood Carol D. johnson Pauline N. Walsh Nicola Witton Julie Green sarah Corkhill University Hospital of North Staffordshire NHS Trust Clinical Education Centre School of Nursing and Midwifery Keele University Stoke-on-Trent UK

This paper outlines a curriculum model for contemporary programme design for the purpose of embedding educational innovation and technology within an inter-professional nursing curriculum. It has been developed from work within the School of Nursing and Midwifery at keele University during the re-write of both the nursing and midwifery curriculum. The Nursing and Midwifery Council (NMC) require approval of all recordable programmes every five years and as such the school took this review as an opportunity to explore the curriculum model currently in use and develop an approach that would facilitate the professional requirements of the programme alongside the embedding of innovative learning and teaching methodologies. The model springs from extensive application of contemporary pedagogy underpinning adult learning, and forces consideration of cognitive alignment within a multi-modal delivery framework [1]. The model builds upon the early work of Fowler and Mayes [2] and later work of Bird [3] who explored the antecedents and underpinning theory for success within online learning experiences. This model has greater reach;having strategic fit for acontemporary “technology enhanced learning” application within further and higher education [4], whilst ensuring the achievement of given professional standards [5].

关键词： Nursing Curriculum Inter-Professional Technology-Enhanced Model keele

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The exploitation of epichloae endophytes for agricultural benefit

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Fungal Diversity 2013年第3期60卷 171-188页

作者： Linda J.johnson Anouck C.M.de Bonth Lyn R.Briggs John R.Caradus sarah C.Finch Damien J.Fleetwood Lester R.Fletcher David E.Hume Richard D.johnson Alison J.Popay Brian A.Tapper Wayne R.Simpson Christine R.Voisey Stuart D.Card AgResearch Ltd Grasslands Research CentreTennent DrivePalmerston North 4442New Zealand AgResearch Ltd Ruakura Research CentreEast StreetHamilton 3214New Zealand AgResearch Ltd Lincoln Research CentreCorner Springs Road and Gerald StreetLincoln 8140New Zealand AgResearch Laboratory of Structural Biology School of Biological SciencesUniversity of Auckland3a Symonds StreetAuckland 1142New Zealand Grasslanz Technology Ltd Tennent DrivePalmerston North 4474New Zealand

Epichloae endophytes of family Clavicipitaceae(comprising genera Epichloëand Neotyphodium)are fungal symbionts of Pooideae *** associations formed,range from mutually beneficial to antagonistic and the nature of this relationship is dependent upon the importance of vertical(via host seeds)versus horizontal(ascospore mediated)transmission of the *** endophytes can enhance their hosts’survival through protection from abiotic and biotic stresses and can thus be utilized in an agricultural ***-safe grass-endophyte associations that confer bio-protective properties for increased pasture persistence and productivity have been developed and *** of the crucial drivers underpinning the selection of epichloae strains for commercial development is endophyte derived *** potential of next generation endophytes is determined by testing a number of attributes such as agronomic fitness,animal and food safety as well as compatibility with host plants of *** research supports these activities by focusing on elucidating mechanisms of compatibility between host and fungal symbiont,as well as investigating other molecular drivers of symbiosis such as siderophore mediated iron-uptake,fungal signalling,fungal growth in host plants and fungal secondary *** review weaves together the different strands of multidisciplinary research aimed at ultimately exploiting epichloae endophytes for increased pasture performance.

关键词： Epichloë Neotyphodium Pooideae Symbiosis Secondary metabolite Toxicology

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