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Evaluation of biostimulation and Tween 80 addition for the bioremediation of long-term DDT-contaminated soil

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Journal of Environmental Sciences 2015年第2期27卷 101-109页

作者： Bibiana Betancur-Corredor Nancy J.Pino santiago Cardona Gustavo A.Peuela GDCON Research Group Faculty of Engineering University Research Headquarters (SIU) University of Antioquia School of Geosciences and Environment Faculty of Mines National University of Colombia School of Microbiology University of Antioquia

The bioremediation of a long-term contaminated soil through biostimulation and surfactant addition was evaluated. The concentrations of 1,1,1-trichloro-2,2-bis（4-chlorophenyl）ethane（DDT） and its metabolites 1,1-dichloro-2,2-bis（4-chlorophenyl） ethane（DDD） and1,1-dichloro-2,2-bis（4-chlorophenyl） ethylene（DDE） were monitored during an 8-week remediation process. Physicochemical characterization of the treated soil was performed before and after the bioremediation process. The isolation and identification of predominant microorganisms during the remediation process were also carried out. The efficiency of detoxification was evaluated after each bioremediation protocol. Humidity and p H and the heterotrophic microorganism count were monitored weekly. The DDT concentration was reduced by 79% after 8 weeks via biostimulation with surfactant addition（B ＋ S） and 94.3%via biostimulation alone（B）. Likewise, the concentrations of the metabolites DDE and DDD were reduced to levels below the quantification limits. The microorganisms isolated during bioremediation were identified as Bacillus thuringiensis, Flavobacterium sp., Cuprivadius sp.,Variovorax soli, Phenylobacterium sp. and Lysobacter sp., among others. Analysis with scanning electron microscopy（SEM） allowed visualization of the colonization patterns of soil particles. The toxicity of the soil before and after bioremediation was evaluated using Vibrio fischeri as a bioluminescent sensor. A decrease in the toxic potential of the soil was verified by the increase of the concentration/effect relationship EC50 to 26.9% and 27.2% for B ＋ S and B, respectively, compared to 0.4% obtained for the soil before treatment and 2.5%by natural attenuation after 8 weeks of treatment.

关键词： DDT Soil Bioremediation Biostimulation Surfactant Toxicity

Extraction of Nuclear Matter Properties from Nuclear Masses by a Model of Equation of State

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Communications in Theoretical Physics 2001年第11期36卷 583-588页

作者： K.C.Chung C.S.Wang A.J.santiago Instituto de Fisica Universidade do Estado do Rio de JaneiroRio de Janeiro-RJ 20559-900Brazil Instituto de FisicaUniversidade do Estado do Rio de JaneiroRio de Janeiro-RJ 20559-900Brazil Instituto de FisicaUniversidade do Estado do Rio de JaneiroRio de Janeiro-RJ 20559-900Brazil

The extraction of nuclear matter properties from measured nuclear masses is investigated in the energy density functional formalism of *** is shown that the volume energy a1 and the nuclear incompressibility Ko depend essentially on μnN -+- pZ - 2EN,whereas the symmetry energy J and the density symmetry coefficient L as well as symmetry incompressibility Ks depend essentially on μn - μp,where μp ＝μp - Ec/ Z,μn and μp are the neutron and proton chemical potentials respectively,EN the nuclear energy,and Ec the Coulomb *** obtained symmetry energy is J ＝ 28.5 MeV,while other coefficients are uncertain within ranges depending on the model of nuclear equation of state.

关键词： nuclear matter properties,nuclear matter equation of state

Irrigation of Romaine Lettuce (Lactuca sativa)Using Wastewater Treated byNon-Conventional Technologies

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World Journal of Engineering and Technology 2017年第5期5卷 1-11页

作者： Manuel Enrique Lopez Sepulveda santiago gutierrez ruiz Antonio Lineiro Bullon Jose Maria Quiroga Alonso Department of Environmental Technologies(Av Republica Saharaui S/N Faculty of Marine and Environmental Sciences(CASEM)CP11510 Puerto RealCadizSpain

The aim of this study was to assess the capacity for reuse of wastewater treated in stabilisation ponds and subsequently reclaimed by means of different filtration systems at pilot scale. An analysis of filtered water showed turbidity values of below 5 NTU, a total suspended solids (TSS) content of 7 mg/l, and Escherichia coli values of up to 1.6 log CFU/100 ml. These results fall within the parameters stipulated in RD 1620/2007 Spanish Water Reuse Regulations governing the reuse of reclaimed wastewater for agricultural purposes. The water reclaimed by means of filtration systems was used to irrigate Romainelettuce (Lactuca sativa longifolia), comparing growth with that of the same variety irrigated with water from the supply network. The results showed a mean difference in lettuce growth of up to 300% in favour of the crop irrigated with reclaimed water.

关键词： Reclamation, Reutilisation, Small Communities, Intermittent Sand Filters,Infiltration-Percolation, Stabilisation Ponds, Artificial Wetlands

Impact of the terrestrial reference frame on the determination of the celestial reference frame

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Geodesy and Geodynamics 2019年第1期10卷 58-71页

作者： Maria Karbon santiago Belda Tobias Nilsson Institute of Geodesy and Geoinformation University of Bonn Image Processing Laboratory (IPL)-Laboratory of Earth Observation (LEO) University of Valencia Lantmateriet

Currently three up-to-date Terrestrial Reference Frames(TRF) are available, the ITRF2014 from IGN, the DTRF2014 from DGFI-TUM, and JTRF2014 from JPL. All use the identical input data of space-geodetic station positions and Earth orientation parameters, but the concept of combining these data is fundamentally different. The IGN approach is based on the combination of technique solutions, while the DGFI is combining the normal equation systems. Both yield in reference epoch coordinates and velocities for a global set of stations. JPL uses a Kalman filter approach, realizing a TRF through weekly time series of geocentric coordinates. As the determination of the CRF is not independent of the TRF and vice versa, the choice of the TRF might impact on the CRF, Within this work we assess this *** find that the estimated Earth orientation parameter(EOP) from DTRF2014 agree best with those from ITRF2014, the EOP resulting from JTRF2014 show besides clear yearly signals also some artifacts linked to certain stations. The estimated source position time series however, agree with each other better than ±1 μas. When fixing EOP and station positions we can see the maximal effect of the TRF on the CRF. Here large systematics in position as well as proper motion arise. In case of ITRF2008 they can be linked to the missing data after 2008. By allowing the EOP and stations to participate in the adjustment,the agreement increases, however, systematics remain.

关键词： Reference frames CRF TRF DTRF2014 JTRF2014 ITRF2014

The change of paradigm in the treatment of HER2-positive breast cancer with the development of newgeneration antibody-drug conjugates

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Cancer Drug Resistance 2023年第1期6卷 45-58页

作者： santiago Escriva-de-Romani Cristina Saura Medical Oncology Service Breast Cancer UnitVall d’Hebron Institute of Oncology(VHIO)Passeig de la Vall d’Hebron119Barcelona 08035Spain

HER2-positive breast cancer is an aggressive *** a result of the development of specific HER2-targeted therapies,such as trastuzumab,more than 20 years ago,the prognosis of these patients has *** HER2-positive breast cancer patients are achieving better survival rates upon treatment with anti-HER2 therapies than patients with HER2-negative *** HER2 blockade with trastuzumab and pertuzumab combined with a taxane achieved an unprecedented survival of over 57 months in first-line *** emtansine,the first antibody-drug conjugate approved for patients in second-line treatment was a potent cytotoxic agent bound to trastuzumab and is currently a standard therapeutic *** the progress in treatment development,most patients develop resistance and eventually *** in the design of antibody-drug conjugates have led to the development of new generation drugs with enhanced properties,such as trastuzumab deruxtecan and trastuzumab duocarmazine,which are significantly changing the paradigm in the treatment of HER2-positive metastatic breast cancer.

关键词： Breast cancer HER2-positive ADCs New drugs Mechanisms of resistance

Traits uncover quasi-neutral community assembly in a coastal heath vegetation

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Journal of Plant Ecology 2019年第4期12卷 703-712页

作者： JoséLuiz Alves Silva Alexandre Fadigas Souza Louis Stephen santiago Programa de Pós-Graduação em Ecologia Departamento de EcologiaUniversidade Federal do Rio Grande do NorteNatal 59078-970Brazil Departamento de Ecologia Universidade Federal do Rio Grande do NorteNatal 59078-970Brazil Department of Botany and Plant Sciences University of California2150 Batchelor HallRiversideCA 92521USA Smithsonian Tropical Research Institute BalboaAncónPanamáRepublic of Panamá

Aims Trait-based approaches are increasingly being used in ecology due to their potential to explain and predict community structure and dynamic regardless of species ***,we asked whether coastal heath vegetation established in stressful habitats could be assembled by quasi-neutral mechanisms based on functional trait *** are organized through quasi-neutral dynamics if abiotic gradients and/or species interactions impose sorting on functional traits and species performances,but not on species phylogenetic relatedness and *** The study was conducted in a coastal area containing tall dunes near the sea,and relatively flat areas punctuated by short palaeodunes further inland,Northeastern South *** evaluated 10 traits of the 21 most abundant species,as well as eight abiotic variables in 70 plots(25 m^(2))established in dune tops,valleys,flat and steep *** effect of environmental filter was tested by linear mixed models(LMM)between each community-weighted mean(CWM)trait and the abiotic variables,and by two indices of environmental filtering based on intra-and interspecific-trait vari-ability relative to null *** evaluate the effect of species inter-actions,we used an index of niche overlap relative to null models,and LMMs between this index and the species richness of *** Findings The environmental filtering index did not deviate from null expect-ations for any *** that included individual variability per-formed similarly to models that used species mean ***,the explicit consideration of environmental gradients by CWM ana-lyses revealed functional patterns that would remain undetected if trait distribution was analyzed *** addition,intraspecific vari-ation in the stem length was an important driver of species diversity as suggested by the relationship between species richness and the niche overlap *** on the occurrence and phylogenetic re-latedness o

关键词： Restinga heath vegetation harsh environment stress-tolerant species response traits

Mainstreaming Disaster Risk Reduction and Climate Change Adaptation in Comprehensive Development Planning of the Cities in Nueva Ecija in the Philippines

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International Journal of Disaster Risk Science 2021年第3期12卷 367-380页

作者： Arneil G.Gabriel Patrick Neil M.santiago Rosemarie R.Casimiro Department of Public Administration Nueva Ecija University of Science and Technology3100 Cabanatuan CityPhilippines

Recent scientific consensus suggests that climate-related disasters are becoming more frequent and ***,increasing importance is given to disaster risk reduction(DRR)and climate change adaptation(CCA)in global *** projected global warming at 1.5℃and the climate variability that the Philippine archipelago experiences make DRR-CCA the key priorities of both the national and local government *** this study,we assessed and measured the degree of mainstreaming of DRR-CCA in the comprehensive development plans(CDPs)of the five component cities in the province of Nueva Ecija in the *** are among the areas in Central Luzon that are susceptible to hydrometeorological and geologic *** distributed survey questionnaires to 25 employees of the local government units in the five component cities in Nueva Ecija who are directly involved in planning *** triangulated their responses using archival data(review of written policies and plans).The main findings of the study indicate that in spite the presence of laws and policies on DRR-CCA,their implementation is only in the preliminary *** results also provide insights to policymakers and future researchers on the challenges and opportunities influencing the systemic mainstreaming of DRR-CCA in the province.

关键词： Climate-related disasters Global warming Local development planning Philippines Southeast Asia

Virtual globes for UAV-based data integration:Sputnik GIS and Google Earth^(TM) applications

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International Journal of Digital Earth 2019年第5期12卷 583-593页

作者： Marcos Arza-Garci Mariluz Gil-Docampo Juan Ortiz-Sanz santiago Martinez-Rodriguez Agroforestry Engineering Department University of Santiago de CompostelaEscuela Politecnica SuperiorLugoSpain

The integration of local measurements and monitoring via global-scale Earth observations has become a new challenge in digital Earth *** increasing accessibility and ease of use of virtual globes(VGs)represent primary advantages of this integration,and the digital Earth scientific community has adopted this technology as one of the main methods for disseminating the results of scientific *** this study,the best VG software for the dissemination and analysis of highresolution UAV(Unmanned Aerial Vehicle)data is identified for global and continuous geographic scope *** VGs Google Earth and Sputnik Geographic Information System(GIS)are selected and compared for this *** Earth is a free platform and one of the most widely used VGs,and one of its best features its ability to provide users with quality visual *** proprietary software Sputnik GIS more closely approximates the analytical capacity of a traditional GIS and provides outstanding advantages,such as DEM overlapping and visualization for its dissemination.

关键词： Virtual reality geospatial data geographic visualization multiscale DEM

Molecular epidemiology of Giardia duodenalis infection in humans in Southern Ethiopia:a triosephosphate isomerase gene-targeted analysis

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Infectious Diseases of Poverty 2018年第1期7卷 179-188页

作者： Mengistu Damitie Zeleke Mekonnen Tadesse Getahun Dante santiago Luc Leyns Department of Environmental Health Sciences and Technology Jimma UniversityJimmaEthiopia School of Medical Laboratory Sciences Jimma UniversityJimmaEthiopia Department of Biology Faculty of Science and Bioengineering SciencesVrije Universiteit BrusselBrusselsBelgium

Background:Giardia duodenalis is a species complex consisting of multiple genetically distinct *** species imposes a major public health crisis on developing ***,the molecular diversity,transmission dynamics and risk factors of the species in these countries are *** study was conducted to determine the molecular epidemiology of *** infection in asymptomatic individuals in Southern ***:From March to June 2014,fresh stool samples were collected from 590 randomly selected ***-demographic data were gathered using a pre-tested structured *** genotyping was done using triosephosphate isomerase gene-based nested polymerase chain reaction and DNA *** genetic identity and relatedness of isolates were determined using the basic local alignment search tool and phylogenetic *** factors associated with *** infection were analysed using binary and multinomial logistic regression ***:The results showed that 18.1%(92/509)of the study subjects were infected by *** the isolates,35.9%(33/92)and 21.7%(20/92)were sub-typed into assemblages A and B,respectively,whereas 42.4%(39/92)showed mixed infections of A and *** of the assemblage A isolates(94%,31/33)were 100%identical to sequences registered in GenBank,of which the majority belonged to sub-assemblage ***,the high genetic variability and frequency of double peaks made sub-genotyping of assemblage B more problematic and only 20%(4/20)of the isolates matched 100%with the *** risk factors of age(P=0.032)and type of drinking water source(P=0.003)both showed a significant association with the occurrence *** ***:This study established the endemicity of *** in Southern *** with assemblage A was more frequent than with assemblage B,and the rate of infection was higher in children and in municipal/tap and open spring water con

关键词： Giardia duodenalis Assemblage Molecular epidemiology Risk factors Triosephosphate isomerase gene Ethiopia