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Identification of necrophagous fly species from 12 different cities in China using ISSR and SCAR markers

Asian Pacific Journal of Tropical Medicine 2010年第7期3卷 510-514页

作者： Xueli Zheng Jialin Hu santhosh puthiya kunnon Chen Xiaoguang Department of Pathogenic Biology School of Public Health and Tropical Medicine Southern Medical University

Objective:To identify necrophagous fly speies from different regions in China using inter simple sequenc repeat(ISSR) and sequence-characterized amplified region(SCAR) melocular markers and to analyze their gene difference and genetic ***:Five carrion fly species were collected from 12 cities and regions in China,including Musca domestica(***), Lucilia sericata(***),Chrysomya megacephala(***),Helicophagella melanura(***),Boethcherisca peregrina,and they were studied using ISSR and SCAR ***:Eight ISSR primers were used for amplification of 121 samples.679 clear and stable bands were identified,of which 516 bands were *** species-specific ISSR fragment were cloned and sequenced as an initial effort to derive the SCAR *** *** SCAR specific primers,SCAR-PCR amplification was performed for 8 *** population sample DNA from different regions in China as well as L sericata,***, *** and Lucillia *** result showed only *** produced specificalty 600 bp fragment,but L sericata,***,*** and Lucillia cupirina did not produce the same specific *** analysis showed clustering of most flies of M. domestica,*** and L *** samples from different regions in China yielded different banding ***:Application of ISSR-PCR and SCAR markers to identify necrophagous fly species from 12 cities and regions in China is first ***-PCR and SCAR markers provide a quick reliable molecular marker technique for the identification of different species of necrophagous fly.

关键词： Forensic insects Necrophagous flies Different population Molecular markers Inter-simple sequence repeat Sequence-characterized amplified region

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